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Elsevier

Books in Biochemistry biophysics

This portfolio spans the molecular mechanisms of life, encompassing protein structure, enzyme function, and biophysical techniques. It supports biochemists, biophysicists, and molecular biologists in understanding complex biological processes. Featuring advanced research, experimental methods, and real-world applications, these resources drive innovations in drug development, disease research, and biomolecular engineering, fostering breakthroughs at the intersection of chemistry, physics, and biology.

  • Molecular Mechanisms of Cytokine and Growth Factor Receptors

    • 1st Edition
    • Volume 751
    • English

  • Molecular Chaperones in Neurodegenerative Diseases

    • 1st Edition
    • Volume 156
    • English
    Molecular Chaperones in Neurodegenerative Diseases explores the roles of molecular chaperones in maintaining protein homeostasis and their involvement in the onset and progression of neurodegenerative disorders. The book highlights current research on chaperone-mediated mechanisms, disease pathology, and emerging therapeutic approaches targeting protein misfolding and aggregation.

  • RNA-Binding Proteins Part D

    • 1st Edition
    • Volume 746
    • English
    A central theme of this volume is the dynamic relationship between RNA structure, RNA-binding proteins, and cellular organization. The chapters integrate experimental, computational, biochemical, biophysical, imaging, and systems-level approaches to investigate how RNA–protein interactions regulate RNA processing, translation, localization, stability, phase separation, and cellular stress responses. As Volume 746 of the Methods in Enzymology series, the volume emphasizes the integration of methodological innovation with biological applications, providing researchers with practical approaches to dissect how RNA–protein interactions govern RNA fate and function, biomolecular condensate formation, and stress granule assembly.Overall, this collection highlights the transition of RNA biology from a primarily sequence-centered discipline toward a more dynamic view in which RNA structure, molecular interactions, phase separation, cellular organization, and signaling are intimately connected. It will therefore be a valuable resource for researchers and students seeking both practical methodologies and mechanistic insights into the rapidly expanding field of RNA biology.

  • Enzymology for Biotechnologists

    • 1st Edition
    • Timir Tripathi + 1 more
    • English
    Enzymology for Biotechnologists provides a comprehensive introduction to enzymology with a strong focus on its applications in biotechnology. Chapters cover the fundamental principles of enzyme structure, function, kinetics, and assays, and extends into advanced topics such as enzyme production, immobilization, reactor design, process intensification, and the use of enzymes in low-water and green solvent systems. Special attention is given to industrial applications, grey and white biotechnology, and the integration of processes that highlight sustainability and innovation. The book presents enzymology from a perspective of biotechnology, and focuses on scale-up, process design, and industrial applications. This targeted approach makes sure that BTech, MTech, MSc, and PhD students, as well as researchers and professionals, gain not only a solid understanding of enzyme science but also its translation into real-world biotechnological processes.

  • Hereditary Syndromes Associated with Endocrine Tumors

    • 1st Edition
    • Volume 134
    • English
    Volume 134, Hereditary Syndromes Associated with Endocrine Tumors, provides a comprehensive and up-to-date overview of the genetic, molecular, clinical, diagnostic, and therapeutic aspects of inherited endocrine neoplasia syndromes. Over the past two decades, remarkable advances in genomics, molecular diagnostics, and precision medicine have transformed the understanding and management of hereditary endocrine tumor syndromes, enabling earlier diagnosis, individualized surveillance strategies, targeted therapies, and improved patient outcomes. This volume brings together contributions from internationally recognized experts to review the current state of knowledge on both well-established and emerging hereditary syndromes affecting the endocrine system. The chapters cover the genetic basis, genotype–phenotype correlations, clinical manifestations, screening recommendations, multidisciplinary management, and future therapeutic perspectives for syndromes predisposing to tumors of the adrenal glands, pituitary, thyroid, parathyroid, pancreas, and neuroendocrine tissues. Special emphasis is placed on recent discoveries in cancer predisposition genes, advances in molecular pathology, the integration of next-generation sequencing into clinical practice, and the implications of hereditary cancer syndromes for patients and their families.

  • Brain-Computer Interfaces (BCI)

    • 1st Edition
    • Volume 229
    • English
    Vitamins and Hormones series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • RNA-binding Proteins Part C

    • 1st Edition
    • Volume 745
    • English

  • Plant Peptides

    Enzymatic Production and Health-Promoting Effects
    • 1st Edition
    • Tolulope Joshua Ashaolu + 2 more
    • English
    Plant Peptides: Enzymatic Production and Health-Promoting Effects provides up-to-date insights on the enzymatic production, bioactivity, and applications of plant-derived peptides. This comprehensive volume explores the role of plant-based proteins (PBPs) as sustainable alternatives to traditional animal proteins, highlighting their environmental and climatic benefits. The book examines the enzymology of proteolytic enzymes used to generate bioactive peptides from PBPs, detailing their bioaccessibility, stability, and bioavailability. It offers an in-depth analysis of the multifarious biological activities of these peptides, including their antioxidant, antimicrobial, and metal-binding properties. The book also addresses the technological and biological properties, limitations, and challenges of plant-based bioactive peptides for industrial uses.With a strong focus on practical implementation, the book discusses emerging strategies and advanced technologies for producing and analyzing bioactive peptides, such as in silico analysis, fermentation, and peptidomics. It provides insights into future research directions and potential applications, emphasizing the health-promoting effects of plant-based peptides in combating obesity, diabetes, hypertension, cancer, and neurodegenerative diseases. This book is an essential resource for food science researchers, nutritionists, and students of food science and nutrition.

  • Translational Insights in Regenerative Medicine

    • 1st Edition
    • Volume 228
    • English
    Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • Mannose Binding Lectin Associated Serine Proteases

    Structure, Function and Therapeutic Implications
    • 1st Edition
    • Krishnan Hajela
    • English
    Mannose Binding Lectin Associated Serine Proteases: Structure, Function and Therapeutic Implications provides a comprehensive overview of MASP-1, MASP-2, and MASP-3, highlighting their roles in immune defense, disease mechanisms, and therapeutic intervention. This book covers the molecular structure and catalytic mechanisms of these key serine proteases, emphasizing their involvement in the lectin pathway of complement activation as well as their emerging functions in coagulation and bradykinin pathways. Chapters detail the biology of animal lectins and collections, classical and alternative complement pathways, and specific biochemical activities of MASP-1, MASP-2, and MASP-3.Readers will find in-depth discussions on the clinical significance of MASP gene polymorphisms and serum levels, and more. Special focus is given to the application of MASP-2 inhibitors in the management of complications such as hematopoietic stem-cell transplantation-asso... thrombotic microangiopathy (HSCT-TMA). This book is an essential resource for researchers in immunology, biochemistry, and molecular medicine, as well as clinicians seeking to advance their understanding of complement biology and the therapeutic potential of targeting the lectin pathway.

  • RNA-Binding Proteins Part B

    • 1st Edition
    • Volume 744
    • English
    Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • Advances in Clinical Chemistry

    • 1st Edition
    • Volume 137
    • English
    Advances in Clinical Chemistry series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • Introduction to Practical EPR Spectroscopy

    • 1st Edition
    • Wilfred R. Hagen
    • English
    Introduction to Practical Electron Paramagnetic Resonance (EPR) Spectroscopy of randomly oriented molecules finds wide application in chemistry, biology, and many other related disciplines. This graduate-level text guides the reader along the winding road of the undocumented pitfalls and unwritten rules-of-thumb that come with undertaking practical EPR spectroscopy. The aim is to provide a truly practical and complete introduction to this widely used method. With the required quantum mechanics limited to an indispensable minimum, the book comprehensively treats the basic theoretical and practical aspects of the methodology to educate the reader to the level of an independent EPR researcher in all aspects, including problem posing, sample preparation, machine operation, data collection and interpretation, numerical analysis, and scientific reporting.Many EPR books have been written as expositions of the quantum-mechanical background of magnetic resonance, but without addressing critical practical questions in the lab including but not limited to:what knobs to turnhow to make sampleswhat programs to use (or not use)how to present experimental resultsIntroduction to Practical EPR Spectroscopy offers a complete preparation (both theoretical and practical) to be an independent EPR spectroscopist in what may be a non-specialist laboratory. It is written primarily for PhD, master’s and postdoctoral students and their tutors across biochemistry, organic, inorganic, bioinorganic and physical chemistry, molecular biology, microbiology, molecular physics, materials science, and geology.

  • Omics Approaches in Rhizosphere Microbiology: Part B

    • 1st Edition
    • Volume 61
    • English
    Omics Approaches in Rhizosphere Microbiology: Part A offers a definitive, systems-level exploration of the complex microenvironment where plant roots and soil microorganisms interact. As global agriculture faces the dual challenges of climate change and food insecurity, understanding the rhizosphere has shifted from traditional culture-dependent isolation to advanced high-throughput molecular auditing. This volume serves as a comprehensive, technically rigorous manual and structural roadmap, bridging foundational rhizosphere biology with the cutting-edge structural analytical tools of modern polyphasic science.

  • RNA-Binding Proteins Part A

    • 1st Edition
    • Volume 743
    • English
    Overall, this volume serves as a comprehensive methodological resource that integrates RNA-binding proteins, RNA metabolism, phase separation, and neurodegenerative disease mechanisms. As Volume 743 of the Methods in Enzymology series, RNA-Binding Proteins highlights recent advances in the field through chapters covering topics such as RNA-Binding Proteins: Structure, Function, and RNA Interactions; RNA Granules, Phase Separation, and Biomolecular Condensates; RNA Regulation in Cellular Physiology; RNA-Binding Proteins in Neurobiology and Neurodegeneration; RNA Processing, Editing, Splicing, and Decay; Non-Coding RNAs, Small RNAs, and RNA Therapeutics; and Experimental and Computational Methods for RNA–Protein Interaction Analysis. Collectively, these contributions provide valuable methodological and conceptual insights, making the volume highly relevant to researchers in molecular biology, neuroscience, RNA biology, and translational medicine.

  • Applications of Microfluidics Device Technology

    • 1st Edition
    • Volume 227
    • English
    Microfluidics is a rapidly expanding field that is recognized as a powerful platform for accelerating biological science research and innovation. It has the potential to reduce the cost and reagent consumption by enabling the precise manipulation of a small amount of fluids through microchannel ranging from ten to hundreds micrometres in size. It is a multidisciplinary domain, which integrates molecular analysis, molecular biology, and microelectronics. It has demonstrated practical applications in the design and fabrication of systems capable of processing low volumes of fluids while enabling multiplexing, automation, and high-throughput screening. Since its emergence in early 1980s, microfluidics has significantly contributed in the development of inkjet printheads, DNA chips, lab-on-a-chip technology, micro-propulsion, and micro-thermal technologies. Microfluidic chips comprise intricate networks of microchannels and chambers designed to manipulate fluids at the microscale. This volume highlights the applications of microfluidics in the detection and analysis of infectious diseases caused by bacteria, viruses, fungi, and protozoa. It also explores the use of microfluidic technologies in the diagnosis and study of non-infectious diseases, including cardiovascular disorders, cancer, metabolic diseases, and neurological conditions. It also covered single-cell analysis, high-throughput drug screening, cell sorting, and both preclinical and clinical applications. It also used in role of microfluidics in organ culture, regenerative medicine, and personalized healthcare. It also addresses ethical and regulatory issues in the development and use of microfluidic devices. This volume is designed to serve as valuable resource for students, researchers, scientists, clinicians, stakeholders, policymakers, and practitioners to understand microfluidics and apply microfluidics technology/devices in various fields. It provides a strong foundation to help the extension of microfluidics knowledge and accelerate research, innovation, technology and practical applications.

  • Pheochromocytoma, Paraganglioma and Adrenocortical Carcinoma: Physiopathology and Diagnosis

    • 1st Edition
    • Volume 133
    • English
    Volume 133 provides a comprehensive and up-to-date overview of three of the most challenging endocrine neoplasms—pheochromo... paraganglioma, and adrenocortical carcinoma. Bringing together contributions from internationally recognized experts, this volume explores the latest advances in tumor biology, genetics, molecular mechanisms, and endocrine pathophysiology, while offering a practical approach to modern diagnostic strategies. Readers will find detailed discussions on hereditary syndromes, genotype–phenotype correlations, biochemical testing, imaging modalities, histopathological assessment, molecular profiling, and emerging biomarkers that are reshaping the diagnosis and risk stratification of adrenal tumors. Integrating cutting-edge scientific discoveries with clinical practice, this volume serves as an essential resource for endocrinologists, oncologists, endocrine surgeons, pathologists, radiologists, nuclear medicine specialists, geneticists, researchers, and trainees seeking to improve the diagnosis and understanding of these rare but clinically significant endocrine malignancies. With its multidisciplinary perspective and strong translational focus, Volume 133 is an indispensable reference for clinicians and scientists dedicated to advancing adrenal tumor care and research.

  • Protein Misfolding

    • 1st Edition
    • Timir Tripathi
    • English
    Protein Misfolding explores the structural and biochemical foundations of protein misfolding, highlighting its wide-reaching implications for cellular function, disease progression, and biomedical innovation. This book provides a comprehensive understanding of how proteins deviate from their native conformations and the resulting biological consequences. Focusing on both physiological and pathological misfolding, the volume covers essential topics such as folding quality control, molecular chaperones, degradation pathways, and proteostasis. It presents in-depth analyses of protein aggregation mechanisms, amyloid formation, and misfolding-associate... diseases including Alzheimer's, Parkinson's, and prion-related disorders. Additional chapters explore structural approaches to understanding misfolded states, computational modeling of aggregation kinetics, and current efforts to design therapeutics that restore folding homeostasis or prevent toxic accumulation. The book also addresses the cellular response to misfolding stress and the integration of folding dynamics with cellular signaling pathways.This book is a valuable resource for researchers, educators, and professionals in biochemistry, structural biology, molecular medicine, biotechnology, and related disciplines. It supports efforts to understand the fundamental principles of protein structure and function, while offering insight into emerging therapeutic strategies and future research directions in disease-focused protein science.

  • Machine Learning in Enzymology

    • 1st Edition
    • Volume 742
    • English
    This volume surveys cutting-edge machine learning–based approaches for the study of enzyme catalysis, dynamics, and design. It brings together computational and experimental approaches that integrate molecular simulation, machine learning, and high-throughput data to interrogate enzymatic catalysis at multiple scales. The chapters in the volume highlight how data-driven frameworks complement physics-based models to advance mechanistic understanding and enable rational enzyme engineering.

  • Hyperspectral Imaging

    • 2nd Edition
    • Jose Manuel Amigo + 1 more
    • English
    Hyperspectral Imaging, Second Edition builds on the foundational insights of the first edition, covering core analytical frameworks and introducing new algorithms, hardware technologies, and cutting-edge applications. By incorporating the latest research findings and case studies, sections provide readers with enhanced tools and techniques for effective hyperspectral and multispectral image analysis across diverse scientific, industrial, and field domains. The book also addresses evolving challenges and fresh opportunities in the field, ensuring that users are equipped with the most current knowledge and practices. It underscores the continued relevance and transformative potential of hyperspectral and multispectral imaging in contemporary research and industry.Graduate students, academics, early researchers, and industry scientists across various disciplines working with hyperspectral and multispectral images, including analytical chemistry, remote sensing, vegetation and crops, food and feed production, forensic sciences, biochemistry, medical imaging, pharmaceutical production, art studies, cultural heritage, and more will find this update extremely useful.

  • Advances in Clinical Chemistry

    • 1st Edition
    • Volume 136
    • English
    Advances in Clinical Chemistry series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • Proteomics and Metabolomics in Drug Discovery

    • 1st Edition
    • Volume 155
    • English
    Proteomics and Metabolomics in Drug Discovery explores how large-scale protein and metabolite analysis is transforming the identification, validation, and development of new therapeutic targets. The book highlights emerging technologies, analytical strategies, and real-world applications that support biomarker discovery, mechanism-of-action studies, and precision medicine approaches in modern drug development.

  • Foundation of Microfluidics Device Technology

    • 1st Edition
    • Volume 226
    • English
    Foundation of Microfluidics Device Technology, Volume 225 presents the fundamentals of microfluidics and microfluidics devices, including computational design and fabrication, artificial intelligence and internet of things for microfluidics devices, materials, surface modifications and functionalization of microfluidics, fabrication of paper based microfluidics. Additional topics include microfluidics for generation of biomaterials, microfluidics for multi-omics analysis, lab-on-the-chip, microfluidics for 2D and 3D cell culture systems, microfluidics for drug and gene delivery, biomimetic models, and the economic considerations associated with microfluidics devices development.Specific chapters in this new release include An introduction to microfluidics and devices, Computational design and modeling of microfluidics devices, Artificial intelligence and IoT for microfluidics devices, Fabrication techniques for design of microfluidics devices, Materials, surface modification and functionalization of microfluidic devices, Fabrication of paper based microfluidics, Microfluidics in biomaterials generation and formulation, Microfluidics for chemical and biological analysis, and more.

  • Methods in Systems Bioinorganic Chemistry

    • 1st Edition
    • Volume 741
    • English
    Methods in Systems Bioinorganic Chemistry, Volume 741 provides understanding of high-level properties involving metals in biology that are founded on molecular or mechanistic level interactions. Currently, there is no established “tool kit” for studying bioinorganic systems, so the methods included in the book span a diverse range of topics and approaches, from imaging to proteomics, from metals-in-mice to mathematical modeling. Specific chapters cover Inductively Coupled Plasma Mass Spectrometry (ICP-MS)-based methods to quantify transition metals in cells and vertebrate models, ICP-MS elemental analysis in tissue samples, and much more.Additional chapters delve into An inductively-coupled-... triple quadrupole method with oxygen for the determination of essential elements and protein concentration, Calculating metal speciation of proteins inside cells, Probing biological copper in the subfemtomolar regime, Using LC-ICP-MS chromatography to detect and characterize Labile Metal Pools, Linking dynamic bioinorganic chemistry processes of toxic metals in the bloodstream to establish their exposure-response relationship in humans, Contextualizing X-ray fluorescent microscopy – high resolution X-rays meet subcellular morphology, Chemoproteomic profiling of transition metal binding sites in bacterial proteomes, Heme Labeling by Proximity (HeLP) to Identify Hemoproteins, and much more.

  • Arginase: Structure, Function, and Therapeutic Targeting

    • 1st Edition
    • Volume 740
    • English
    Arginase: Structure, Function, and Therapeutic Targeting, Volume 740 in the Methods in Enzymology series, provides a comprehensive, methodology-driven perspective on arginase research spanning molecular, biochemical, and translational domains. Chapters in this new release include Expression, Purification, and Structural Characterization of Human and Leishmania Arginase with Comparative Analysis of Structures from the Protein Data Bank, Synthesis of First-Generation Arginase Inhibitors, Computational and Structure-Guided Design of Arginase Inhibitors, Metal Substitution in Arginase: Experimental Approaches to Study Metal-Dependent Catalysis, Therapeutic Arginase Formulations: PEGylation, Stability, and Advanced Delivery Systems, and much more.Additional chapters cover Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization, Synthesis of Second-Generation Arginase Inhibitors, Cryo-EM Workflow and Structural Characterization of Arginase–Antibody Complexes, Development and Validation of an Electrochemical Assay for Arginase Activity, Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion, Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives, Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB, Beyond Arginase: Structural Biology of the Extended Guanidinase Family, Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism, and much more.

  • The Metabolon

    • 1st Edition
    • Volume 739
    • English
    Methods in Enzymology series: The Metabolon, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • Advances in Biomembranes and Lipid Self-Assembly

    • 1st Edition
    • Volume 44
    • English
    Advances in Biomembranes and Lipid Self-Assembly, Volume 43 highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Content in this new release covers Engineered probiotics for producing phosphoinositol-sn-l... as biosurfactant modulators: Their hydropathy, in potentiating surface tension and scaffold properties into model emulsions.

  • Structural Biology of Ubiquitination

    From Mechanisms to Interventions
    • 1st Edition
    • Navaneet Chaturvedi + 1 more
    • English
    Structural Biology of Ubiquitination: From Mechanisms to Interventions provides an in-depth exploration of the structural and biophysical principles governing ubiquitination and their implications in diseases such as cancer and neurodegeneration. The book seamlessly integrates experimental insights with advanced computational methods, including AI-driven drug discovery, molecular modeling, and biophysical simulations, offering a comprehensive toolkit for researchers and clinicians. By bridging fundamental science with translational applications, the book empowers readers to design targeted therapeutics, understand disease mechanisms at the molecular level, and accelerate progress toward personalized medicine.

  • Flavoproteins and Flavoenzymes

    • 1st Edition
    • Volume 738
    • English
    Flavoproteins and Flavoenzymes, Volume 738 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Kirisu2 and KiPaD: global analysis of rapid flavoprotein redox kinetics from stopped-flow multi, ilagros Mendina, A Generic First Approach to Dissecting Flavoenzyme Catalytic Cycles, Removing Covalent Constraints of Protein Loops and Lids by a Split-Gene Strategy, Designing Oxygen Reaction Sites in Flavoprotein Dehydrogenases, Converting Noncovalent Flavoproteins into Covalently Flavinylated Proteins, Complexities of Riboflavin Biosynthesis, Reactions of Riboflavin Catabolism, and much more.Additional sections cover Ferredoxin-dependent thioredoxin reductase, Thermophilic Methylenetetrahydrof... Reductases, Ion-Pumping NADH:Quinone Oxidoreductase, Loading E. coli Dihydroorotate Dehydrogenase with Coenzyme Q, and Single-Component Halogenases.

  • Applied Enzymology and Biocatalysis

    Mechanistic Principles for Industrial and Clinical Applications
    • 1st Edition
    • Muhammad Sajid Hamid Akash + 2 more
    • English
    Applied Enzymology and Biocatalysis: Mechanistic Principles for Industrial and Clinical Applications examines the molecular mechanisms, kinetics, and engineering of enzymes that underpin their growing use across biotechnology, medicine, and industry. The book provides a comprehensive exploration of how enzymatic processes can be optimized and adapted to meet the evolving needs of biomanufacturing, sustainable chemistry, and therapeutic innovation. With a clear emphasis on mechanistic insight and translational relevance, the book covers enzyme structure–function relationships, catalytic mechanisms, protein engineering, directed evolution, and computational design. It further integrates applications in biocatalysis, nanobiotechnology, biosensor development, green chemistry, and synthetic biology. Special attention is given to the clinical and diagnostic uses of enzymes, including their role in drug discovery, metabolic engineering, and precision healthcare. This book serves as a detailed reference for understanding the principles and applied aspects of enzyme science in modern biotechnology. It is a valuable resource for biochemists, molecular biologists, biotechnologists, pharmaceutical researchers, process engineers, and graduate students seeking to expand their expertise in enzymology and biocatalytic innovation.

  • Super-Resolution Nanoscopy for Protein Imaging

    • 1st Edition
    • Varun Vyas
    • English
    Super-Resolution Nanoscopy for Protein Imaging decodes the intricate world of protein visualization using cutting-edge nanoscopic techniques. This comprehensive guide is crafted to equip master students, PhD candidates, and early-stage researchers with essential knowledge and practical insights into advanced protein imaging technologies. The book covers foundational principles, innovative methodologies, and real-world applications, providing a complete resource for mastering the complexities of protein imaging. This book offers a unique compilation of diverse nanoscopic methods, overcoming the limitation of technique-specific texts currently available.By collecting scattered information into a single volume, it delivers an in-depth exploration of imaging techniques, from Atomic Force Microscopy (AFM) to Electron Microscopy (EM), and Single Molecule Fluorescence Imaging. Each section delves into instrumentation, sample preparation, data analysis, and the latest developments, making it an indispensable reference for understanding how to visualize proteins in their natural environments.

  • Enzymes for Seaweed Polysaccharide Degradation and Industrial Applications

    Unlocking Biocatalytic Potential for Sustainable Innovation
    • 1st Edition
    • Yasmin Khambhaty
    • English
    Enzymes for Seaweed Polysaccharide Degradation and Industrial Applications: Unlocking Biocatalytic Potential for Sustainable Innovation provides an in-depth exploration of the biochemistry and enzymology of marine polysaccharides and the enzymes responsible for their degradation. Focusing on compounds such as agar, carrageenan, alginate, fucoidan, and laminarin, the book investigates their structural properties, biological functions, and the enzymatic mechanisms involved in their breakdown. Chapters cover microbial sources of these enzymes, biochemical pathways, and the molecular basis of enzyme-substrate specificity. Particular attention is given to the potential biomedical and nutraceutical relevance of the resulting oligosaccharides, including their immunomodulatory, antiviral, and anti-inflammatory properties. Advances in enzyme engineering and synthetic biology approaches are discussed to illustrate how enzymatic systems can be optimized for targeted applications. This book is an essential reference for biochemists, enzymologists, and molecular bioscientists interested in marine biochemistry, carbohydrate-active enzymes, and bioactive compounds with therapeutic potential. By integrating fundamental enzymology with emerging applications relevant to human health, it supports the development of novel bio-based solutions in medicine and nutrition.

  • Enzyme Microfluidics

    • 1st Edition
    • Volume 737
    • English
    Enzyme Microfluidics, Volume 737 in the Methods in Enzymology series, presents detailed methodological descriptions and protocols that cover a wide range of microfluidics-based approaches for mechanistic enzymology, high-throughput screening, and enzyme engineering campaigns. The volume includes both established methods as well as advances in the field. Sections in this new release cover four unique sections, including Chips and Reagents, Enzyme Characterization, Enzyme Engineering, and Moving Beyond Microfluidics (from micro to nanoscale). Specific chapters delve into Joint Methods for Fabricating Microfluidic Chips, A liquid handling robotic workflow for quantifying the activity of antibiotic resistance genes (ARGs) in cell-free protein synthesis, and much more.Additional chapters cover Preparation of uniform microcrystals by droplet microfluidics for time-resolved serial crystallography, Droplet microfluidic HDX for investigating highly dynamic proteins, An Accessible, Low-Cost Platform for High-Throughput Microfluidic Enzyme Kinetics, High-Throughput Data Processing, DA-MEK, Mass Spectrometry of Droplets, Digital Bioassay Using a Femtoliter Reactor Array Device, Confocal absorbance droplet sorting, Sequence-Function Data Analysis from Microfluidics Screening, and much more.

  • Bio-Click Chemistry

    Expanding Horizons Beyond the Click
    • 1st Edition
    • Flavia Zacconi + 2 more
    • English
    Bio-Click Chemistry: Expanding Horizons Beyond the Click delves into the dynamic intersection of biocatalysis and click chemistry, providing a comprehensive exploration of these rapidly evolving fields. This book offers in-depth insights into both historical perspectives and the latest advancements, ensuring readers gain a thorough understanding of fundamental principles, practical applications, and future directions. Readers will discover the versatile applications of biocatalysis and click chemistry in pharmaceuticals, biotechnology, biology, biomedicine, nanomedicine, and materials science, equipping researchers, educators, and industry professionals with the tools and techniques needed to foster innovation and real-world impact. Furthermore, the book investigates the emerging trends, challenges, and opportunities in biocatalysis and click chemistry, empowering readers to stay ahead of the curve and drive transformative developments in chemical synthesis and enzymatic engineering. This indispensable guide is designed for researchers, educators, and industry professionals dedicated to advancing their work and exploring new frontiers in chemical synthesis and biocatalytic processes.

  • Ecology of Marine Rocky Shores

    • 1st Edition
    • Volume 105
    • English
    Ecology of Marine Rocky Shores, Volume 105 in the Advances in Marine Biology series, highlights new advances in the field, presenting comprehensive chapters written by an international board of authors. This volume explores key ecological processes shaping rocky shore environments, including the impacts of plastic pollution, the dynamics of warm-temperate and subtropical systems, and the roles of foundation species and community interactions.Additio... chapters examine conservation planning, kelp ecology and restoration, fisheries and indigenous knowledge, and the influence of ocean rafting on species distribution. The volume also addresses spatial and network approaches to ecology, coastal marine microbiology, and the ecological impacts of invasive species. Together, these contributions provide an integrated perspective on the structure, function, and resilience of rocky shore ecosystems, offering valuable insights for researchers, marine ecologists, and environmental scientists.

  • Advances in Clinical Chemistry

    • 1st Edition
    • Volume 135
    • English
    Advances in Clinical Chemistry series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

  • Microbial Biofilms and Cancer - Part B

    • 1st Edition
    • Volume 225
    • English
    Biofilms in Cancer: Molecular Mechanisms, Host Reprogramming and Tumor Progression presents a focused and multidisciplinary examination of the emerging role of microbial biofilms in cancer biology. The volume challenges the conventional view of biofilms as merely persistent microbial communities and highlights their potential contribution to chronic inflammation, host-cell reprogramming, tumor development, cancer progression, and invasion.The book begins by establishing biofilms as an underestimated threat in cancer, providing a foundation for understanding the complex interactions between microbial communities and malignant tissues. It subsequently explores the molecular mechanisms through which quorum sensing and biofilm-associated signals can influence host-cell behavior, together with the mechanisms underlying biofilm formation and microbial interaction with host cells.A major focus of the volume is the biofilm-mediated modulation of the tumor microenvironment. The chapters examine how biofilm components can dysregulate cytokines and chemokines, sustain chronic inflammatory responses, and create conditions that may favor tumorigenesis. Particular attention is given to the transition from persistent microbial colonization and inflammation to malignant transformation and tumor progression.The book also provides organism- and cancer-specific perspectives, with detailed discussions of the contribution of Porphyromonas gingivalis and Candida biofilms to oral squamous cell carcinoma, as well as the involvement of Helicobacter pylori, Fusobacterium nucleatum, P. gingivalis, Escherichia coli, and Candida across specific cancer contexts. These chapters highlight the diversity of microbial strategies and host responses associated with biofilm-linked malignancies.The concluding perspective addresses the emerging role of biofilms in cancer invasion, bringing together microbial persistence, host-cell interaction, inflammatory signaling, and tumor progression into an integrated framework. Collectively, the volume provides a comprehensive overview of the biofilm–host–inflamm... axis, emphasizing how microbial communities may influence different stages of cancer biology.This book will be valuable to microbiologists, cancer biologists, oncologists, molecular biologists, immunologists, oral health researchers, infectious-disease specialists, biotechnologists, clinicians, and postgraduate researchers seeking to understand the increasingly important intersection of microbial biofilms and cancer. It also provides a foundation for identifying new research questions, biomarkers, preventive strategies, and potential therapeutic targets at the interface of infection and malignancy.

  • The Matrix Metalloproteinases

    • 1st Edition
    • Volume 60
    • English
    The Matrix Metalloproteinases, Volume 60 in The Enzymes series, explores recent advances in the study of matrix metalloproteinases (MMPs), a critical family of enzymes involved in extracellular matrix remodeling and numerous physiological and pathological processes. This volume presents a comprehensive collection of chapters covering key aspects of MMP biology, including their structure, function, regulation, and detection through activity-based probes.Chapters in this release examine classical and selective MMP inhibitors, including specific targeting strategies for MMP-13, as well as the role of endogenous tissue inhibitors of metalloproteinases. Additional contributions explore the involvement of MMPs in various biological and disease contexts, including oral diseases, neurodegenerative disorders, neutrophil biology, and cancer progression.The volume also addresses current challenges and opportunities in the design of MMP-targeted therapeutics, highlighting advances in drug development and pharmacological applications. Together, these chapters provide valuable insights into the biochemical, physiological, and clinical significance of MMPs, making this volume an essential reference for researchers in enzymology, biochemistry, molecular biology, and biomedical sciences.

  • Protein Kinase Inhibitors

    The Promise of Natural Products in Cancer Therapeutics
    • 1st Edition
    • Md. Imtaiyaz Hassan + 1 more
    • English
    Protein Kinase Inhibitors: The Promise of Natural Products in Cancer Therapeutics explores a novel therapeutic avenue for cancer treatment through nutraceutical-based kinase inhibitors. The book covers their historical background, outlook, mechanistic pathways, and clinical aspects, highlighting their relevance in cancer therapy. It also examines the potential of polyphenolic derivatives and other natural compounds in inhibiting key kinases involved in major cancer signaling pathways, focusing on their application in clinical trials and marketed drugs. Sections explore the implications of polyphenols in cancer management, novel sources of breast cancer inhibitors, the role of natural products in lymphoma therapy, and the multitarget potential of phytochemicals. Each chapter is designed to provide a thorough understanding of how natural products can be harnessed to develop innovative cancer therapeutics. It will serve as a valuable resource for academics, scientists, young researchers, students, and pharmacists working or interested in kinase biology and cancer therapeutics with its unique perspective on using nature's own arsenal to combat cancer.

  • Enzyme Technology for Environmental Sustainability, Waste to Wealth, and Circular Economy

    • 1st Edition
    • Shrikaant Kulkarni
    • English
    Enzyme Technology for Environmental Sustainability, Waste to Wealth, and Circular Economy offers a comprehensive look at how enzyme technology can drive environmental progress within the framework of biochemistry. Beginning with foundational concepts, the book introduces readers to the science behind enzymes and their practical applications. Each chapter builds upon these basics, illustrating how enzymes catalyze vital processes that contribute to sustainability and the transformation of waste into valuable resources. Through focused case studies and clear explanations, the book demonstrates the potential of enzyme-driven solutions to address urgent environmental challenges and promote a circular economy.Beyond its central focus, the book stands out by delivering pragmatic examples that remain within biochemical boundaries, avoiding broader discussions in public health or environmental science. It is designed as a valuable reference for biochemists, researchers, and industry professionals, emphasizing collaboration and innovation rooted in biochemical expertise. Readers will gain an enriched understanding of enzyme technology’s role in sustainable industrial practices and the future of environmentally responsible solutions.

  • Carrier-Free Enzyme Immobilization

    Fundamentals and Innovations in Biocatalysis
    • 1st Edition
    • Mehdi Mohammadi + 1 more
    • English
    Carrier-Free Enzyme Immobilization: Fundamentals and Innovations in Biocatalysis explores innovative techniques for immobilizing enzymes without the use of inert carriers, highlighting their advantages, applications, and integration into modern biocatalytic processes. The book begins by introducing the concept of enzyme immobilization and the transition from traditional carrier-bound methods to innovative carrier-free techniques. It discusses the fundamental principles behind carrier-free immobilization, including the types of cross-linkers used and the advantages in enhancing enzyme stability and activity. The book also covers various methods for the structural characterization of carrier-free immobilized enzymes.With a strong focus on practical implementation, it examines the applications of carrier-free immobilized enzymes across different industries, highlighting the pros and cons of the method. It explores novel approaches for producing carrier-free immobilized biocatalysts and provides a comparative analysis of carrier-free versus traditional carrier-bound immobilization techniques. The book also addresses the challenges associated with implementing carrier-free immobilization in industrial processes and proposes potential solutions to overcome these obstacles. It is an essential resource for biochemists, chemical engineers, and researchers in biotechnology who are involved in enzyme research and development.

  • Thiamine, thiamine pyrophosphate and thiamine pyrophosphate-dependent enzymes Part B

    • 1st Edition
    • Volume 736
    • English
    Thiamine Pyrophosphate (TPP)-Dependent Enzymes, Volume 736 in the Methods in Enzymology series covers this diverse group of ubiquitous enzymes with timely chapters on Methods for Studying ThDP-dependent Mycobacterial MenDs (SEPHCHC Synthases), Mammalian 2-hydroxyacyl-CoA lyases, discovery and assays, Anti-infective target 1-deoxy-d-xylulose-5... synthase (DXS) and drug design for enzyme inhibition, Expression, Purification, and Mechanistic Studies of DXP synthases from Deinococcus radiodurans, Plasmodium falciparum, and Plasmodium vivax, Comparison of the catalysis of ThPP-dependent enzymes AHAS, ALS, and DXS, and much more.Additional sections cover Plant and fungal acetohydroxyacid synthases (AHAS): Methods for heterologous expression, kinetic characterization, and structural analysis, Biochemical and Structural characterization of novel ‘split-gene’ archeal transketolases, 4-Hydroxybenzoylform... decarboxylase enzymes from Rhodococcus jostii RHA1 and Pseudomonas fluorescens Pf-5: characterization and involvement in bacterial lignin degradation, Engineering of phosphoketolase from Bifidobacterium adolescentis for increased activity on non-phosphorylated substrates and application in a cell-free reaction system for ATP regeneration, Repurposing TPP-dependent enzymes with anodic oxidation for the conversion of aldehydes to enantioenriched carboxylic acids, and much more.

  • Fundamentals of Absorption Spectroscopy

    • 1st Edition
    • Imtaiyaz Hassan
    • English
    Fundamentals of Absorption Spectroscopy offers a clear and comprehensive introduction to the principles, instrumentation, and applications of absorption spectroscopy across UV-Visible, IR, and other spectral regions. Designed for students, researchers, and industry professionals, this book bridges theory and practice with practical illustrations and real-world case studies, providing a solid foundation in both fundamental concepts and contemporary applications in chemistry, biology, medicine, environmental monitoring, and materials science. It fills the gap between overly advanced and overly simplified texts by combining fundamental theory with practical insights, particularly from biological perspectives. This book addresses the need for an updated, student-friendly, and application-oriented resource in absorption spectroscopy. Readers will benefit from structured guidance that connects theoretical principles with experimental data, explains instrumentation and its limitations, and demonstrates applications across diverse fields such as pharmaceuticals, environmental analysis, and biomolecular sciences. The inclusion of case studies and recent advances in spectroscopic technology ensures relevance for both academic study and professional practice.

  • Microbial Biofilms and Cancer - Part A

    • 1st Edition
    • Volume 224
    • English
    This book provides a comprehensive and multidisciplinary exploration of the emerging biological connection between microbial biofilms, chronic infection, genetic instability, and cancer development and progression. Moving beyond the traditional perception of biofilms as merely microbial communities associated with antimicrobial resistance, the book examines biofilms as dynamic biological ecosystems capable of influencing host signaling, immune responses, genome stability, epigenetic regulation, cellular metabolism, and oncogenic processes.The opening chapters establish the molecular and genetic foundations of biofilm-associated carcinogenesis. The role of horizontal gene transfer within biofilms and its potential oncogenic consequences is examined alongside genetic profiling of biofilm-associated cancers. Particular emphasis is placed on the signaling pathways that may be hijacked by biofilms and the epigenetic mechanisms governing biofilm biology and host–microbe interactions. The book further explores how biofilms can modulate antitumor immunity and contribute to cancer progression, while genetic instability and biofilm-driven mutations are considered as potential mechanisms linking persistent microbial colonization with malignant transformation.A further dimension of the book focuses on the therapeutic and translational potential of biofilm biology. Bacterial metabolites are examined as emerging sources of anticancer molecules and as potential tools for developing innovative cancer chemotherapeutics. At the same time, the book critically addresses the dual nature of biofilms, considering their potential applications alongside associated biological risks, environmental consequences, biosafety concerns, and legal challenges.The latter part of the book introduces an emerging AI- and nanotechnology-enabl... paradigm for infection–cancer surveillance and diagnostics. Advanced nanostructured biosensors, AI-assisted multiplexed platforms, metaheuristic optimization, and integrated surveillance technologies are presented as approaches for simultaneous detection and monitoring of infectious and cancer-associated biomarkers. The application of AI to wastewater surveillance further expands the discussion toward a One Health framework, connecting environmental pathogen monitoring with potential cancer-risk assessment.The final contribution integrates genomics, metagenomics, and artificial intelligence to investigate infection-associated genomic signatures in Bacteroides fragilis, demonstrating how multi-omics and computational approaches can facilitate biomarker discovery and improve understanding of infection–cancer relationships.Collec... the book brings together microbiology, molecular oncology, genomics, epigenetics, immunology, metabolomics, nanotechnology, biosensing, artificial intelligence, environmental surveillance, and One Health. It provides an integrated perspective on the emerging biofilm–infection–ca... axis, highlighting both fundamental mechanisms and opportunities for diagnosis, prevention and therapeutic intervention.The book is intended for researchers, academicians, clinicians, biotechnologists, microbiologists, cancer biologists, bioinformaticians, nanotechnologists, environmental scientists, and advanced postgraduate students interested in the rapidly evolving interface between microbial biology and cancer.

  • Advances in Marine Biology

    • 1st Edition
    • Volume 104
    • English
    Advances in Marine Biology, Volume 104, the latest release in this serial that highlights new advances in the field, includes comprehensive chapters written by an international board of authors. Chapters in this new release explore Integrative Fish Biomechanics and Advancing marine invertebrate biodiversity in South Africa: Addressing gaps in taxonomy and genetic knowledge.

  • Thiamine and Thiamine-Pyrophosphate (TPP) Riboswitches

    • 1st Edition
    • Volume 735
    • English
    This volume of "Methods in Enzymology" series is devoted to the cofactor thiamine, also known as vitamin B1, and its phosphorylated derivatives. The biosynthesis of thiamine is discussed in terms of key enzymes and their regulation, the evolution of metabolic pathways, and its importance in plant and yeast metabolism. The importance of quantifying and distinguishing between phosphorylated and unphosphorylated thiamine in animal and human tissues using chromatographic, enzymatic, and nanopore methods is also discussed. In addition to these methods, thiamine riboswitches are a major focus of this book, covering everything from their identification in plants and the elucidation of their structure to their application in detection.This book presents state-of-the-art methods and detailed protocols intended for all researchers interested in thiamine metabolism or its detection.

  • Alternative Proteins

    • 1st Edition
    • Volume 154
    • English
    Alternative Proteins, Volume 154 provides a comprehensive overview of alternative proteins, exploring their sources, production technologies, nutritional profiles, and potential to transform the global food system. Covering plant-based, microbial, insect, and cultured (cell-based) proteins, the book examines the scientific advances, market trends, regulatory challenges, and sustainability implications of these innovative protein sources. Chapters in this new release include Introduction to Alternative Proteins, Molecular Properties of Plant-Based Proteins for Alternative Proteins, The Science of Alternative Proteins: Comparing Animal and Plant-Based Sources, Blue Proteins: Marine-Derived Alternatives for Sustainable Nutrition and Climate-Resilient Food Systems, and much more. Additional sections cover Insect Proteins for Alternative Proteins: Sustainable Solutions with Molecular Efficiency, Plant-Based Proteins: Key Sources and Production Methods, Fungal proteins as Eco-friendly Alternate Proteins, Algae and Microbial Proteins for Alternative Proteins: Untapped Molecular Resources, Integrating Siddha Dietary Principles with Alternative Protein Systems: A Pathway Toward Sustainable and Functional Nutrition, Alternative Proteins: Structure and Functional Properties, and a variety of extensive and timely topics.

  • Cyclodextrin Glucanotransferase

    A Comprehensive Guide from Gene to Applications
    • 1st Edition
    • Kiransinh Rajput
    • English
    Cyclodextrin Glucanotransferase: A Comprehensive Guide from Gene to Applications explores the essential role of cyclodextrin glucanotransferase (CGTase) in starch degradation and its diverse industrial applications. This book serves as a valuable resource for researchers and industry professionals, offering comprehensive insights from the genetic basis of CGTase to its practical applications. The book covers the production of CGTase by different bacterial genera, including Bacillus spp., and their purification strategies. It delves into the structural and functional characteristics of CGTase, highlighting its catalytic mechanisms, substrate specificity, and factors affecting enzyme activity and stability. Additionally, the book explores recombinant CGTases, their properties, and advancements in protein engineering and enzyme immobilization. With a strong focus on practical applications, the book discusses the remarkable properties of cyclodextrins, such as inclusion complex formation, and their wide range of uses in various industries, including pharmaceuticals, textiles, bioremediation, and food. Detailed case studies and real-world examples illustrate how CGTase and cyclodextrins can address specific challenges and drive innovation in these fields.This book is an essential resource for applied science researchers working in the field of starch processing enzymes, providing them with the knowledge to advance their research and development. It also serves as a valuable reference for researchers in the starch processing industries, offering insights into the latest advancements and practical applications of CGTase and cyclodextrins.

  • Recent Advances in Targeting Inflammation

    Ion Channels, GPCR Signaling Tools, Therapies, and Beyond
    • 1st Edition
    • Senthilkumar Rajagopal + 3 more
    • English
    Recent Advances in Targeting Inflammation: Ion Channels, GPCR Signaling Tools, Therapies, and Beyond delves into the multifaceted occurrences of inflammation across different systems, such as neuroinflammation and gastric inflammation. The book's main goal is to present the latest highlights, covering advancements in inflammation, investigational therapies, animal models, and biomarkers. These insights are crucial for better diagnosis, monitoring, and potential therapeutic options for patients suffering from both acute and chronic inflammation. This comprehensive work explores various topics, including ion channel regulation in inflammation, GPCR regulation, signaling pathways, and therapeutic approaches.It emphasizes the importance of understanding the role of ion channels, GPCR, and nutrition in managing inflammation effectively. Additionally, the book discusses the global impact of chronic inflammation on health outcomes, stressing the need for improved healthcare planning and strategies.

  • Histone Deacetylases in Neurodegenerative Disease Part B

    • 1st Edition
    • Volume 734
    • English
    Histone Deacetylases in Neurodegenerative Disease, Part B, Volume 734 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on timely topics surrounding the Interaction of HDAC6 ZnF UBP with Tau affects its stability by NMR and Simulation studies, Protocols for In Vitro Analysis of Liquid-Liquid Phase Separation of Proteins, Histone deacetylases in NS, Histone Deacetylase 6 ZnF UBP domain enhances podosome formation in neuronal cells studied by high-resolution confocal microscopy, Methodological approach to Investigate Peroxiredoxin 1 Deacetylation by Histone Deacetylase 6, Critical structural features of HDAC11 inhibitors, and much more.Additional chapters in this release include Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration, Elucidation of Site-Specific Ubiquitination on Chaperones in Response to Mutant Huntingtin, Protein acetylation dynamics (histone and non-histone) and role protein deacetylases in disease, Epigenetic Clues: Predicting Maternal Depression Through DNA Methylation, A short guide on in vivo transfection of flag-HDAC4 in dentate gyrus for study of adult neurogenesis in brain trauma, Decoding the Acetylation Journey from Neural Crest to Melanocyte, and more.

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