Skip to main content
Elsevier

Books in Enzymology

  • Cold-Adapted Enzymes

    Biochemistry and Biotechnology
    • 1st Edition
    • Diego Gomez-Casati + 1 more
    • English
    Cold-Adapted Enzymes: Biochemistry and Biotechnology provides a cutting-edge investigation into the molecular mechanisms, structural dynamics, and regulatory pathways of enzymes adapted to cold environments.Beyond classical microbiology, the book explores cold adaptation from psychrophilic bacteria and Antarctic fungi to microalgae, plants, and mammalian cell physiology. It provides biochemistry insights into carbohydrate (amylases, glucoamylases) and lipid metabolism, proteases, and redox enzymes, alongside the roles of molecular chaperones and prokaryotic proteomics. A key highlight is its focus on next-generation biotechnology, featuring AI-driven protein design, advanced computational modeling, and structural bioinformatics. Bridging foundational science with market reality, the volume explores applications from organic waste valorization to industrial biocatalysis, analyzing the global patent landscape and commercial players.As the latest volume in the Foundations and Frontiers of Enzymology series, this book is an indispensable reference for researchers across biochemistry, biotechnology, structural biology, and microbiology.

  • Protein-Based Hydrogels

    Exploring the Roles of Protein Hydrogels in Membrane-less Organelles, Disease Mechanisms, and Therapeutic Applications
    • 1st Edition
    • Vladimir N. Uversky + 1 more
    • English
    Protein-Based Hydrogels: Exploring the Roles of Protein Hydrogels in Membrane-less Organelles, Disease Mechanisms, and Therapeutic Applications presents a comprehensive overview of natural and engineered protein hydrogels, examining their critical roles in cellular organization, disease pathology, and therapeutic innovation. This book contributes unique value by integrating fundamental biology, cutting-edge biotechnology, and translational biomedical science to address emerging questions about protein phase separation and gelation. Structured into three sections, the book begins by exploring the physiological functions of protein hydrogels in phase-separated organelles, cellular stress sensing, memory formation, and compartmentalization... It then investigates the pathological consequences of hydrogel dysregulation, including their roles in neurodegenerative diseases, cancer, and viral infections. Finally, it examines biomedical applications such as controlled drug delivery, regenerative medicine, biosensing, and the design of synthetic organelles. Each chapter is contributed by a leading expert, offering multidisciplinary perspectives from biochemistry, molecular biology, bioengineering, and medical research. This book is a valuable resource for academic researchers, graduate students, and professionals working in protein engineering, structural biology, molecular medicine, and pharmaceutical sciences. It supports readers in understanding the full spectrum of protein hydrogel biology—from cell physiology to disease mechanisms and therapeutic design—making it especially relevant for those interested in neurodegeneration, cancer biology, and translational bioengineering.

  • Enzymology for Biotechnologists

    • 1st Edition
    • Timir Tripathi + 1 more
    • English
    Enzymology for Biotechnologists provides a comprehensive introduction to enzymology with a strong focus on its applications in biotechnology. Chapters cover the fundamental principles of enzyme structure, function, kinetics, and assays, and extends into advanced topics such as enzyme production, immobilization, reactor design, process intensification, and the use of enzymes in low-water and green solvent systems. Special attention is given to industrial applications, grey and white biotechnology, and the integration of processes that highlight sustainability and innovation. The book presents enzymology from a perspective of biotechnology, and focuses on scale-up, process design, and industrial applications. This targeted approach makes sure that BTech, MTech, MSc, and PhD students, as well as researchers and professionals, gain not only a solid understanding of enzyme science but also its translation into real-world biotechnological processes.

  • Plant Peptides

    Enzymatic Production and Health-Promoting Effects
    • 1st Edition
    • Tolulope Joshua Ashaolu + 2 more
    • English
    Plant Peptides: Enzymatic Production and Health-Promoting Effects provides up-to-date insights on the enzymatic production, bioactivity, and applications of plant-derived peptides. This comprehensive volume explores the role of plant-based proteins (PBPs) as sustainable alternatives to traditional animal proteins, highlighting their environmental and climatic benefits. The book examines the enzymology of proteolytic enzymes used to generate bioactive peptides from PBPs, detailing their bioaccessibility, stability, and bioavailability. It offers an in-depth analysis of the multifarious biological activities of these peptides, including their antioxidant, antimicrobial, and metal-binding properties. The book also addresses the technological and biological properties, limitations, and challenges of plant-based bioactive peptides for industrial uses.With a strong focus on practical implementation, the book discusses emerging strategies and advanced technologies for producing and analyzing bioactive peptides, such as in silico analysis, fermentation, and peptidomics. It provides insights into future research directions and potential applications, emphasizing the health-promoting effects of plant-based peptides in combating obesity, diabetes, hypertension, cancer, and neurodegenerative diseases. This book is an essential resource for food science researchers, nutritionists, and students of food science and nutrition.

  • Mannose Binding Lectin Associated Serine Proteases

    Structure, Function and Therapeutic Implications
    • 1st Edition
    • Krishnan Hajela
    • English
    Mannose Binding Lectin Associated Serine Proteases: Structure, Function and Therapeutic Implications provides a comprehensive overview of MASP-1, MASP-2, and MASP-3, highlighting their roles in immune defense, disease mechanisms, and therapeutic intervention. This book covers the molecular structure and catalytic mechanisms of these key serine proteases, emphasizing their involvement in the lectin pathway of complement activation as well as their emerging functions in coagulation and bradykinin pathways. Chapters detail the biology of animal lectins and collections, classical and alternative complement pathways, and specific biochemical activities of MASP-1, MASP-2, and MASP-3.Readers will find in-depth discussions on the clinical significance of MASP gene polymorphisms and serum levels, and more. Special focus is given to the application of MASP-2 inhibitors in the management of complications such as hematopoietic stem-cell transplantation-asso... thrombotic microangiopathy (HSCT-TMA). This book is an essential resource for researchers in immunology, biochemistry, and molecular medicine, as well as clinicians seeking to advance their understanding of complement biology and the therapeutic potential of targeting the lectin pathway.

  • Protein Misfolding

    • 1st Edition
    • Timir Tripathi
    • English
    Protein Misfolding explores the structural and biochemical foundations of protein misfolding, highlighting its wide-reaching implications for cellular function, disease progression, and biomedical innovation. This book provides a comprehensive understanding of how proteins deviate from their native conformations and the resulting biological consequences. Focusing on both physiological and pathological misfolding, the volume covers essential topics such as folding quality control, molecular chaperones, degradation pathways, and proteostasis. It presents in-depth analyses of protein aggregation mechanisms, amyloid formation, and misfolding-associate... diseases including Alzheimer's, Parkinson's, and prion-related disorders. Additional chapters explore structural approaches to understanding misfolded states, computational modeling of aggregation kinetics, and current efforts to design therapeutics that restore folding homeostasis or prevent toxic accumulation. The book also addresses the cellular response to misfolding stress and the integration of folding dynamics with cellular signaling pathways.This book is a valuable resource for researchers, educators, and professionals in biochemistry, structural biology, molecular medicine, biotechnology, and related disciplines. It supports efforts to understand the fundamental principles of protein structure and function, while offering insight into emerging therapeutic strategies and future research directions in disease-focused protein science.

  • Applied Enzymology and Biocatalysis

    Mechanistic Principles for Industrial and Clinical Applications
    • 1st Edition
    • Muhammad Sajid Hamid Akash + 2 more
    • English
    Applied Enzymology and Biocatalysis: Mechanistic Principles for Industrial and Clinical Applications examines the molecular mechanisms, kinetics, and engineering of enzymes that underpin their growing use across biotechnology, medicine, and industry. The book provides a comprehensive exploration of how enzymatic processes can be optimized and adapted to meet the evolving needs of biomanufacturing, sustainable chemistry, and therapeutic innovation. With a clear emphasis on mechanistic insight and translational relevance, the book covers enzyme structure–function relationships, catalytic mechanisms, protein engineering, directed evolution, and computational design. It further integrates applications in biocatalysis, nanobiotechnology, biosensor development, green chemistry, and synthetic biology. Special attention is given to the clinical and diagnostic uses of enzymes, including their role in drug discovery, metabolic engineering, and precision healthcare. This book serves as a detailed reference for understanding the principles and applied aspects of enzyme science in modern biotechnology. It is a valuable resource for biochemists, molecular biologists, biotechnologists, pharmaceutical researchers, process engineers, and graduate students seeking to expand their expertise in enzymology and biocatalytic innovation.

  • Super-Resolution Nanoscopy for Protein Imaging

    • 1st Edition
    • Varun Vyas
    • English
    Super-Resolution Nanoscopy for Protein Imaging decodes the intricate world of protein visualization using cutting-edge nanoscopic techniques. This comprehensive guide is crafted to equip master students, PhD candidates, and early-stage researchers with essential knowledge and practical insights into advanced protein imaging technologies. The book covers foundational principles, innovative methodologies, and real-world applications, providing a complete resource for mastering the complexities of protein imaging. This book offers a unique compilation of diverse nanoscopic methods, overcoming the limitation of technique-specific texts currently available.By collecting scattered information into a single volume, it delivers an in-depth exploration of imaging techniques, from Atomic Force Microscopy (AFM) to Electron Microscopy (EM), and Single Molecule Fluorescence Imaging. Each section delves into instrumentation, sample preparation, data analysis, and the latest developments, making it an indispensable reference for understanding how to visualize proteins in their natural environments.

  • Enzymes for Seaweed Polysaccharide Degradation and Industrial Applications

    Unlocking Biocatalytic Potential for Sustainable Innovation
    • 1st Edition
    • Yasmin Khambhaty
    • English
    Enzymes for Seaweed Polysaccharide Degradation and Industrial Applications: Unlocking Biocatalytic Potential for Sustainable Innovation provides an in-depth exploration of the biochemistry and enzymology of marine polysaccharides and the enzymes responsible for their degradation. Focusing on compounds such as agar, carrageenan, alginate, fucoidan, and laminarin, the book investigates their structural properties, biological functions, and the enzymatic mechanisms involved in their breakdown. Chapters cover microbial sources of these enzymes, biochemical pathways, and the molecular basis of enzyme-substrate specificity. Particular attention is given to the potential biomedical and nutraceutical relevance of the resulting oligosaccharides, including their immunomodulatory, antiviral, and anti-inflammatory properties. Advances in enzyme engineering and synthetic biology approaches are discussed to illustrate how enzymatic systems can be optimized for targeted applications. This book is an essential reference for biochemists, enzymologists, and molecular bioscientists interested in marine biochemistry, carbohydrate-active enzymes, and bioactive compounds with therapeutic potential. By integrating fundamental enzymology with emerging applications relevant to human health, it supports the development of novel bio-based solutions in medicine and nutrition.

  • Bio-Click Chemistry

    Expanding Horizons Beyond the Click
    • 1st Edition
    • Flavia Zacconi + 2 more
    • English
    Bio-Click Chemistry: Expanding Horizons Beyond the Click delves into the dynamic intersection of biocatalysis and click chemistry, providing a comprehensive exploration of these rapidly evolving fields. This book offers in-depth insights into both historical perspectives and the latest advancements, ensuring readers gain a thorough understanding of fundamental principles, practical applications, and future directions. Readers will discover the versatile applications of biocatalysis and click chemistry in pharmaceuticals, biotechnology, biology, biomedicine, nanomedicine, and materials science, equipping researchers, educators, and industry professionals with the tools and techniques needed to foster innovation and real-world impact. Furthermore, the book investigates the emerging trends, challenges, and opportunities in biocatalysis and click chemistry, empowering readers to stay ahead of the curve and drive transformative developments in chemical synthesis and enzymatic engineering. This indispensable guide is designed for researchers, educators, and industry professionals dedicated to advancing their work and exploring new frontiers in chemical synthesis and biocatalytic processes.

  • Protein Kinase Inhibitors

    The Promise of Natural Products in Cancer Therapeutics
    • 1st Edition
    • Md. Imtaiyaz Hassan + 1 more
    • English
    Protein Kinase Inhibitors: The Promise of Natural Products in Cancer Therapeutics explores a novel therapeutic avenue for cancer treatment through nutraceutical-based kinase inhibitors. The book covers their historical background, outlook, mechanistic pathways, and clinical aspects, highlighting their relevance in cancer therapy. It also examines the potential of polyphenolic derivatives and other natural compounds in inhibiting key kinases involved in major cancer signaling pathways, focusing on their application in clinical trials and marketed drugs. Sections explore the implications of polyphenols in cancer management, novel sources of breast cancer inhibitors, the role of natural products in lymphoma therapy, and the multitarget potential of phytochemicals. Each chapter is designed to provide a thorough understanding of how natural products can be harnessed to develop innovative cancer therapeutics. It will serve as a valuable resource for academics, scientists, young researchers, students, and pharmacists working or interested in kinase biology and cancer therapeutics with its unique perspective on using nature's own arsenal to combat cancer.

  • Cyclodextrin Glucanotransferase

    A Comprehensive Guide from Gene to Applications
    • 1st Edition
    • Kiransinh Rajput
    • English
    Cyclodextrin Glucanotransferase: A Comprehensive Guide from Gene to Applications explores the essential role of cyclodextrin glucanotransferase (CGTase) in starch degradation and its diverse industrial applications. This book serves as a valuable resource for researchers and industry professionals, offering comprehensive insights from the genetic basis of CGTase to its practical applications. The book covers the production of CGTase by different bacterial genera, including Bacillus spp., and their purification strategies. It delves into the structural and functional characteristics of CGTase, highlighting its catalytic mechanisms, substrate specificity, and factors affecting enzyme activity and stability. Additionally, the book explores recombinant CGTases, their properties, and advancements in protein engineering and enzyme immobilization. With a strong focus on practical applications, the book discusses the remarkable properties of cyclodextrins, such as inclusion complex formation, and their wide range of uses in various industries, including pharmaceuticals, textiles, bioremediation, and food. Detailed case studies and real-world examples illustrate how CGTase and cyclodextrins can address specific challenges and drive innovation in these fields.This book is an essential resource for applied science researchers working in the field of starch processing enzymes, providing them with the knowledge to advance their research and development. It also serves as a valuable reference for researchers in the starch processing industries, offering insights into the latest advancements and practical applications of CGTase and cyclodextrins.

  • Microbial Enzymes

    Classification, Biochemistry, Production and Applications
    • 1st Edition
    • Abdel Moneim Elhadi Sulieman
    • English
    Microbial Enzymes: Classification, Biochemistry, Production and Applications explores the multifaceted roles of microbial enzymes, emphasizing their stability, ease of handling, and manipulation, all of which make them invaluable in various industrial processes. With 25 in-depth chapters, the book delves into the biochemistry of enzymes, their various sources, and their importance in biotechnology. It covers modern techniques for microbial enzyme detection and determination, methods of enzyme immobilization, and the application of microbial enzymes in industries such as food, medicine, pharmaceuticals, and textiles. Special sections discuss the biosynthesis of metal nanoparticles by microbial enzymes and the role of microbial toxins in different sectors. The book's chapters are organized in a way that provides detailed insights into the classification of enzymes, the mechanisms of microbial enzymes, and their industrial importance. Topics include bacterial, fungal, and algal enzymes, their applications, and the latest advancements in enzyme technology. Other sections address the challenge of antibiotic resistance and explores innovative methods for enzyme production and molecular engineering.

  • Recent Trends in Enzyme Immobilization

    • 1st Edition
    • Shailendra Kumar Arya + 3 more
    • English
    Recent Trends in Enzyme Immobilization explores the forefront of enzyme technology, providing a comprehensive guide on the latest methodologies and advancements. This book begins by setting the stage with foundational insights into enzyme immobilization, then progresses to discuss innovative techniques and their applications across various industries, including biopharmaceuticals, environmental science, and food processing. Structured to cater to both newcomers and seasoned professionals in biotechnology and biochemical engineering, each chapter delivers in-depth analysis and up-to-date research findings. Readers will find valuable resources in the practical guidance offered for optimizing enzyme stabilization, activity, and reusability in industrial applications. Moreover, the text addresses the theoretical underpinnings that frame current practices and illuminates future directions for research and technological advancements. Designed as an indispensable resource, this book aims to bridge the gap between academic research and practical application. It equips researchers, practitioners, and advanced students with the necessary tools and knowledge to drive innovation in enzyme immobilization, fostering the development of sustainable solutions and novel approaches in their work. With contributions from leading global experts, Recent Trends in Enzyme Immobilization serves as a critical reference that promises to inspire and guide readers through the complexities and potentials of this dynamic field.

  • Enzymatic Production of Oligosaccharides

    From Basic Research to Industrial Production
    • 1st Edition
    • Carlos Vera + 2 more
    • English
    Enzymatic Production of Oligosaccharides: From Basic Research to Industrial Production, a volume in the Foundations and Frontiers of Enzymology series, covers the enzymes used for the extraction, synthesis, or modification of oligosaccharides, the biochemical properties of those enzymes, and potential technological applications. The book is divided into three parts, the first of which shares the fundamentals on the enzymatic synthesis of oligosaccharides. The book then explores the enzymatic production of oligosaccharides, considering a range of different oligosaccharides and production processes. Final sections cover the technological properties of oligosaccharides and potential applications across the chemical, biochemical, biotechnology, and food industries.This is a useful reference for researchers working with oligosaccharides across biochemistry, enzymology, biotechnology, food chemistry and related fields.

  • Handbook of Proteolytic Enzymes

    Metallopeptidases
    • 4th Edition
    • Neil D. Rawlings + 1 more
    • English
    Handbook of Proteolytic Enzymes, Metallopeptidases has stood as most comprehensive work in the field of applied enzymology and biocatalysis since the first edition published in 1998. Extensively revised and updated, the new fourth edition is an essential reference for biochemists, biotechnologists, and molecular biologists across academia and industry. Edited by world-renowned experts in the field, and with five volumes available for individual sale, this work provides detailed information on all known proteolytic enzymes researched to-date, with expanded coverage of metallopeptidases, cysteine peptidases, serine and threonine peptidases, aspartic and glutamic peptidases, and inhibitors of proteolytic enzymes.This volume includes over 300 chapters on known metallopeptidases enzymes, including their name, history, activity and specificity, structural chemistry, preparation, biological aspects, and distinguishing features, with 2D structures of peptidases in color, extensive references, and links to PubMed and MEROPS databases.

  • Cryptic Enzymes and Moonlighting Proteins

    • 1st Edition
    • Helen Irving + 2 more
    • English
    Cryptic Enzymes and Moonlighting Proteins, a new volume in the Foundations and Frontiers in Enzymology series, offers a thorough overview of cryptic enzymes and moonlighting proteins in signaling cascades. In early chapters, leading international contributors discuss evolutionary considerations for moonlighting proteins, moonlighting interactions in the extracellular matrix, eukaryotic moonlighting proteins, modulating, moonlighting kinases, moonlighting proteins in neurobiology signaling, metabolic enzymes moonlighting as RNA binding and regulatory proteins. Later, methods-driven chapters discuss practical aspects of identifying hidden moonlighting domains in proteins, computational approaches and bioinformatic tools for the identification of cryptic enzymes, establishing cryptic enzyme interactomes, and assessing contributions of moonlighting proteins to signal cascades.The book also explores recent advances in research and brings together an array of information across different fields to enable better targeting of these exciting proteins and their interactomes. With a clear focus on the role of moonlighting and cryptic enzymes in signal transduction, users will find examples of cryptic enzymes across species, as well as those in human healthy biology and pathogenesis.

  • Whole Cell Biocatalysis

    Fundamentals and Applications
    • 1st Edition
    • Sergio Huerta-Ochoa + 2 more
    • English
    Whole Cell Biocatalysis, a volume in the Foundations and Frontiers of Enzymology series, offers a detailed overview of the process of biocatalysis using whole cells as an alternative to enzyme biocatalysis. The book examines the potential applications and advantages of whole cell biocatalysis, including its use in the production of fine chemicals, renewable energy, and drug discovery and development. Whole cell biocatalysis for large scale production and non-conventional media are also covered. In addition, the latest methods and techniques are investigated, including cell immobilization, permeabilization, synthetic biology, computational metabolic engineering, and molecular genetics.This book provides a comprehensive summary on whole cell biocatalysis and the latest developments in this emerging field. It is an invaluable reference for researchers working across biochemistry, enzymology, biotechnology, and related fields.

  • Mitochondrial Transplantation and Transfer

    Biology, Methods, Applications, and Disease
    • 1st Edition
    • Gokhan Burcin Kubat + 2 more
    • English
    Mitochondrial Transplantation and Transfer: Biology, Methods, Applications, and Disease, a volume in the Translational and Applied Bioenergetics series, offers a detailed overview of mitochondrial transplantation across fundamental biology, disease and early therapeutics research. Here, more than twenty experts in the field discuss transplantation and transfer of healthy mitochondria and define future steps to improve new therapeutic applications across a variety of diseases. Emphasis is placed on the physiology of mitochondrial transfer, isolation processes and their applications, transfer methods, and diagnostic and treatment use across cardiovascular, metabolic, and neurological diseases, among other disease types. Step-by-step instruction is provided around research protocols and methods discussed, along with careful attention to the ethical implications of mitochondrial transplantation research and application.

  • Translational Glycobiology in Human Health and Disease

    • 1st Edition
    • Michelle Kilcoyne + 1 more
    • English
    Along with nucleic acids, proteins, and lipids, carbohydrates stand as one of four main components of cellular architecture. However, glycobiology (or carbohydrate bioscience) is little understood by non-experts, partly because carbohydrates are a complex, diverse class of molecules structurally and functionally. In recent years, advances in computational analytics (glycomics) have allowed us to better interpret and realize the importance of glycobiology in human health and disease, and glycans and their associated processes have been shown to play a significant role across a variety of disease types. As the biomedical sciences continue to adopt multi-omic and precision medicine approaches, a greater understanding of glycobiology is essential for maintaining healthy physiology and advancing disease treatment.Translatio... Glycobiology in Human Health and Disease offers a deep examination of glycobiology for experts and non-experts alike in areas ranging from the role of glycobiology in chronic and infectious diseases to advances in technologies for higher throughput analysis and diagnosis. While keeping human health in the forefront, this book integrates a thorough discussion of glycobiology fundamentals with its growing areas of application and societal impact. With emphasis throughout on the interdisciplinary nature of glycosciences, this book also features perspectives from the health, computational (glycoanalytics), materials, biopharmaceutical, and diagnostic sciences.Disease and speciality areas addressed include gycoimmunology, neuroglycobiology, commensal glycobiology, gut health, regenerative medicine and glycobiology, glycobiology and cancer, congenital disorders of glycosylation, infectious disease glycobiology, and parasite glycobiology. Computational approaches discussed, supporting the advance of new research, include advanced glycoanalytics, glycomics microarrays, glycoengineering, and glycol systems biology. Additionally, authors consider impact areas for society and public health, such as glycobiology and entrepreneurship, policy and regulatory requirements for glycosylation, future research, and translation to new diagnostics and drug discovery.

  • Cyanobacteria

    Metabolisms to Molecules
    • 1st Edition
    • Arun Kumar Mishra + 1 more
    • English
    Cyanobacteria: Metabolisms to Molecules covers diversity, fundamental metabolisms, crucial metabolities, their synthesis, and bioinformatics topics surrounding cyanobacteria, an organism regarded as the most important organism to sustain life on the planet during ancient times. Cyanobacteria are among the first pioneering communities on various harsh habitat, hydrarch or xerarch, which finally facilitate the emergence of vast communities including higher plants. Being the progenitor of chloroplast, the cyanobacterial metabolisms has always fascinated microbiologists. Additionally, the ability of these prokaryotes to produce valuable and prolific sources of natural products signified their role in an array of industrial sectors. Therefore, in this book, the book's authors accumulate the current knowledge of cyanobacterial metabolisms and molecules as a valuable asset for students, researchers, and biotechnologists.

  • Metalloenzymes

    From Bench to Bedside
    • 1st Edition
    • Claudiu T. Supuran + 1 more
    • English
    Metalloenzymes: From Bench to Bedside offers a thorough overview of metalloenzymes, spanning biochemical and structural features, pharmacology, and biotechnological applications. After a brief overview, international experts in the field discuss a wide range of magnesium, calcium, zinc, manganese, nickel, iron, copper, cadmium, molybdenum, and tungsten enzymes, along with catalytic roles within their active sites. With a uniform approach throughout, each chapter includes the structure and function of the enzyme, physiologic and pathologic roles, inhibitors and activators of the enzyme (and their design), and clinical agents or compounds applied in medicine and drug discovery. This book enables scientists across academia and industry to adopt ongoing metalloenzyme research, and continuous discovery of novel metalloenzymes, in new life science studies and clinical applications.

  • Polysaccharide Degrading Biocatalysts

    • 1st Edition
    • Rosana Goldbeck + 1 more
    • English
    The transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis. Polysaccharide-Degra... Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrad... enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.

  • Biocatalyst Immobilization

    Foundations and Applications
    • 1st Edition
    • Maria Lujan Ferreira
    • English
    Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

  • Biocatalysis in Green Solvents

    • 1st Edition
    • Pedro Lozano
    • English
    Biocatalysis in Green Solvents offers a pragmatic overview and instruction in biocatalysis and enzymology of green solvents for sustainable industries and medicine, running from concept to application. Here, international experts in the field discuss structure-function relationships of enzymes in ionic liquids (ILs) and examine how enzymes act as selective catalysts for fine biochemical synthesis in non-aqueous environments. Several integral green biochemical processes of biocatalytic transformation and pure product separation are described in detail. Application focused chapters discuss the role of biocatalysis in creating and implementing deep eutectic solvents, biomass derived solvents, sub and supercritical fluids, carbon dioxide biphasic systems, and enzymatic membrane reactors, as well as applying these biocatalytic processes in drug discovery and production.

  • Microbial Xylanolytic Enzymes

    • 1st Edition
    • Pratima Bajpai
    • English
    Microbial Xylanolytic Enzymes describes the enzyme structure and its interaction with plant cell walls, the properties and production of different enzymes and their applications, and the knowledge gathered on the hydrolysis mechanism of hemicellulose. The knowledge gathered about the hydrolysis mechanism of the hemicelluloses, especially xylans, has greatly promoted the rapid application of these enzymes in new areas. In recent years, there has been a spurt of interest in xylan degrading enzymes due to their applications in several industrial processes, including paper and pulp industries, food and feed industries, biofuel industry, textile industry, chemical and pharmaceutical industry, brewing industry, and more. Xylan is the principal type of hemicellulose. An enzymatic complex is responsible for the hydrolysis of xylan, but the main enzymes involved are enzymes produced by fungi, bacteria, yeast, algae, protozoans, and more.

  • Redox Chemistry and Biology of Thiols

    • 1st Edition
    • Beatriz Alvarez + 3 more
    • English
    Redox Chemistry and Biology of Thiols offers an applied, comprehensive overview of redox chemistry and biology of thiol-dependent processes. Running from basic biology and chemistry of redox phenomena to research methods and highlighting recently identified roles of thiols across cellular and bodily systems, this book draws upon a range of disciplines to illuminate new research directions, new applications of thiol studies, and clinical translation. Sections cover thiol oxidizing species, thiol reactivity and modifications, thioredoxin, glutaredoxin, glutathione, peroxidases, thiol repair enzymes, thiol oxidative signaling, disulfide bond formation, thiol-based redox biosensors, cysteine and hydrogen sulfide metabolism, iron-sulfur cluster biogenesis, thiols in chloroplasts, blood thiols, sugar and polyamine thiols in pathogenic organisms, redox medicine (therapeutic applications of thiols and drug development), as well as methods and bioinformatics tools.

  • Cholesterol

    From Chemistry and Biophysics to the Clinic
    • 1st Edition
    • Anna N. Bukiya + 1 more
    • English
    With Cholesterol, Drs. Anna Bukiya and Alex Dopico have compiled a comprehensive resource on biological and clinical aspects of cholesterol, spanning biophysics and biochemistry, as well as the latest pharmacological discoveries employed to tackle disorders associated with abnormal cholesterol levels. Early chapters on basic biology offer guidance in cholesterol lab chemistry, cholesterol metabolism and synthesis, molecular evolution of cholesterol and sterols, cholesterol peptides, and cholesterol modulation. Chapters on cellular and organismal development discuss cholesterol transport in blood, lipoproteins, and cholesterol metabolism; cholesterol detection in the blood; cellular cholesterol levels; hypercholesterolemia... and the role of cholesterol in early human development. Pathophysical specialists consider familial hypobetalipoproteine... critical illness and cholesterol levels, coronary artery disease, CESD, cholesterol and viral pathology, cholesterol and neurodegenerative disorders, and cholesterol and substance use disorders. A final section examines pharmacology of drug delivery systems targeting cholesterol related disorders, cholesterol receptors, cholesterol reduction, statins, citrate lyase, cyclodextrins, and clinical management. Cholesterol: From Biophysics and Biochemistry to Pathology and Pharmacology empowers researchers, students, and clinicians across various disciplines to advance new cholesterol-based studies, improve clinical management, and drive drug discovery.

  • RNA Therapeutics

    The Evolving Landscape of RNA Therapeutics
    • 1st Edition
    • Paloma H. Giangrande + 2 more
    • English
    RNA Therapeutics: The Evolving Landscape of RNA Therapeutics provides a comprehensive overview of RNA therapeutic modalities, from bench-to-bedside, with an emphasis on the increasingly impactful areas of gene therapy, oligonucleotide therapeutics, gene editing and delivery. International leaders in the field examine RNA-based therapeutics tools that have been developed to-date to modulate cellular processes such as transcription, translation and protein function. Approved RNA-based therapies and lessons learned from failed therapies are discussed in-depth, as are evolving advances in RNA biochemical analysis, and similar advances that are enabling clinical application of RNA-based therapies. Later sections discuss delivery technologies, remaining hurdles in research and translation, the therapy development process from the lab to the clinic, and novel RNA-based therapies currently in development.

  • Metabolomics Perspectives

    From Theory to Practical Application
    • 1st Edition
    • Jacopo Troisi
    • English
    Metabolomics Perspectives: From Theory to Practical Application is an expertly written volume, which provides a thorough description of the current state-of-the-art in the metabolomics field. The philosophy behind the book is to guide the reader in a step-by-step exploration of metabolomics experiments, ranging from sample preparation to data extraction, analysis and interpretation, and to discuss the main current applications and future perspectives of this emerging science. Armed with critical insights, coupled with a clear writing, the book consists of three main sections. The first one introduces the pivotal theoretical fundamentals and provides a comprehensive overview of the "wet" laboratory workflow, including protocol instructions and a detailed description of experimental methods and analytical techniques. The second section covers a wide range of topics in the context of data analysis, including guidance in exploratory analysis, supervised and unsupervised machine learning approaches and validation and optimization methods. In addition to the several examples reported in the text, the book features an R package, specifically designed to perform all the described algorithms, which is hosted on a companion website (www.metabolomicsper... together with several sets of available metabolomic data. Finally, an extensive dissertation describes the latest advances and the major fields of interest for metabolomics applications, highlighting their crucial potentials for future biomedical research. Thus, this book represents a must-read for both experienced researchers, interested in metabolomics, and newcomers to the field.

  • Proteolytic Signaling in Health and Disease

    • 1st Edition
    • Andre Zelanis
    • English
    In recent years, powered by evolving technologies and experimental design, studies have better illuminated the regulating role of proteolytic enzymes across human development and pathologies. Proteolytic Signaling in Health and Disease provides an in-depth discussion of fundamental physiological and developmental processes regulated by proteases, from protein turnover and autophagy to antigen processing and presentation and major histocompatibility complex (MHC) molecules. Moving on from basic biology, international chapter authors examine a range of pathological conditions associated with proteolysis, including inflammation, wound healing, and cancer. Later chapters discuss the newly discovered network of connected events among proteases (and their inhibitors), the so-called ‘protease web’, and how best to study it. This book also empowers new research with up-to-date analytical methods and step-by-step protocols for studying proteolytic signaling events.

  • Polymeric Supports for Enzyme Immobilization

    Opportunities and Applications
    • 1st Edition
    • Alka Dwevedi
    • English
    Enzyme immobilization has been approached for finding solutions for various critical problems associated with industries, medicine, environment, agriculture, etc. Especially since last decades, several innovative researches have come up to look for enhancing catalytic efficiency, reusability of immobilized enzyme and longer stability by introducing range of immobilizing supports, supports modifiers through introduction of several chemical agents (non-toxic) and adopting innovative enzyme immobilization methods. In the present book, polymeric supports have been focussed for enzyme immobilization, especially due to their versatility in immobilizing different enzymes for different large scale enzyme reactors to be used for several applications. Especially, polymers can be modified according to applications and enzyme properties which have made it supports of choice for all several enzyme based applications. Polymeric Supports for Enzyme Immobilization: Opportunities and Applications offers in-depth discussions of known polymeric enzyme support materials, reaction processes, and optimized methods to enhance enzyme immobilization. Case-based chapters examine methods of enzyme immobilization onto various polymeric supports, their surface chemistry and physical morphology followed by implementation of polymers based immobilized enzymes in various applications, viz. medicine, environment, industries, clean energy, disease diagnosis, drug delivery etc. This book has prime focus to allow several researchers across the world to provide updated technological details and incite to contribute more innovative work in coming years to find solutions to several critical problems.

  • Biologically Active Peptides

    From Basic Science to Applications for Human Health
    • 1st Edition
    • Fidel Toldra + 1 more
    • English
    Biologically Active Peptides: From Basic Science to Applications for Human Health stands as a comprehensive resource on bioactive peptide science and applications. With contributions from more than thirty global experts, topics discussed include bioactive peptide science, structure-activity relationships, best practices for their study and production, and their applications. In the interdisciplinary field of bioactive peptides, this book bridges the gap between basic peptide chemistry and human physiology, while reviewing recent advances in peptide analysis and characterization. Methods and technology-driven chapters offer step-by-step guidance in peptide preparation from different source materials, bioactivity assays, analysis and identification of bioactive peptides, encoding bioactive peptides. Later, applications across disease areas and medical specialties are examined in-depth, including the use of bioactive peptides in treating obesity, diabetes, osteoporosis, mental health disorders, food allergies, and joint health, among other disorders, as well as bioactive peptides for sensory enhancement, sports and clinical nutrition, lowering cholesterol, improving cardiovascular health, and driving advances in biotechnology.

  • Protocols in Biochemistry and Clinical Biochemistry

    • 1st Edition
    • Shweta Pandey + 2 more
    • English
    Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.

  • Advanced Methods in Molecular Biology and Biotechnology

    A Practical Lab Manual
    • 1st Edition
    • Khalid Z. Masoodi + 2 more
    • English
    Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

  • Lipid Signaling and Metabolism

    • 1st Edition
    • James M. Ntambi
    • English
    Lipid Signaling and Metabolism provides foundational knowledge and methods to examine lipid metabolism and bioactive lipid signaling mediators that regulate a broad spectrum of biological processes and disease states. Here, world-renowned investigators offer a basic examination of general lipid, metabolism, intracellular lipid storage and utilization that is followed by an in-depth discussion of lipid signaling and metabolism across disease areas, including obesity, diabetes, fatty liver disease, inflammation, cancer, cardiovascular disease and mood-related disorders. Throughout, authors demonstrate how expanding our understanding of lipid mediators in metabolism and signaling enables opportunities for novel therapeutics. Emphasis is placed on bioactive lipid metabolism and research that has been impacted by new technologies and their new potential to transform precision medicine.

  • Vibrational Spectroscopy in Protein Research

    From Purified Proteins to Aggregates and Assemblies
    • 1st Edition
    • Yukihiro Ozaki + 3 more
    • English
    Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.

  • Mitochondrial Disease Genes Compendium

    From Genes to Clinical Manifestations
    • 1st Edition
    • Marni J. Falk
    • English
    The field of Mitochondrial Medicine has been dominated by symptom constellation-based diagnostic categorization since the first clinical syndrome was described three decades ago. Now, as rapidly expanding knowledge has revealed that mitochondrial diseases may result from several hundred distinct gene disorders with extensive clinical and mutation heterogeneity, the most useful guide for clinical care and research embraces a gene-centric approach to each individual’s disorder. Together with international colleagues, Dr. Marni Falk has developed the Mitochondrial Disease Sequence Data Resource (MSeqDR), an online, community curated, centralized data resource of mitochondrial disease data from a genomic perspective. Here, in the Mitochondrial Disease Genes Compendium, Dr. Marni Falk and a team of international experts have built off their work on MSeqDR to provide an all-in-one, readily accessible, and easy-to-use at point of care reference on 256 mitochondrial disease genes from a gene-based perspective. In this book, clinicians and researchers will find a complete overview of mitochondrial disease genes relevant across all specialties, cataloging and building context around clinical features and the genetic basis of each condition. Within, each "gene page" offers an in-depth, referenced view of the relevant clinical disease spectrum, including gene and protein descriptions, year discovered, inheritance pattern(s), age ranges affected, major clinical features and severity range, clinical pearls, known therapies, available support groups, animal models, and gene-specific basic, translational, or clinical research activities now underway. Links provided on each gene page direct readers to MSeqDR for new findings, up-to-date genomic variant data, and user friendly informatics tools accessible to general clinicians and sophisticated geneticists or bioinformaticians alike, ensuring access to updated information on each condition.

  • Protein Homeostasis Diseases

    Mechanisms and Novel Therapies
    • 1st Edition
    • Angel L. Pey
    • English
    Protein Homeostasis Diseases: Mechanisms and Novel Therapies offers an interdisciplinary examination of the fundamental aspects, biochemistry and molecular biology of protein homeostasis disease, including the use of natural and pharmacological small molecules to treat common and rare protein homeostasis disorders. Contributions from international experts discuss the biochemical and genetic components of protein homeostasis disorders, the mechanisms by which genetic variants may cause loss-of-function and gain-of-toxic-functi... and how natural ligands can restore protein function and homeostasis in genetic diseases. Applied chapters provide guidance on employing high throughput sequencing and screening methodologies to develop pharmacological chaperones and repurpose approved drugs to treat protein homeostasis disorders.

  • Oxidative Stress

    Eustress and Distress
    • 1st Edition
    • Helmut Sies
    • English
    Oxidative Stress: Eustress and Distress presents current knowledge on oxidative stress within the framework of redox biology and translational medicine. It describes eustress and distress in molecular terms and with novel imaging and chemogenetic approaches in four sections: A conceptual framework for studying oxidative stress. Processes and oxidative stress responses. Signaling in major enzyme systems (oxidative eustress), and damaging modification of biomolecules (oxidative distress). The exposome addresses lifelong exposure and impact on health, nutrient sensing, exercise and environmental pollution. Health and disease processes, including ischemia-reperfusion injury, developmental and psychological disorders, hepatic encephalopathy, skeletal muscle disorders, pulmonary disease, gut disease, organ fibrosis, and cancer. Oxidative Stress: Eustress and Distress is an informative resource useful for active researchers and students in biochemistry, molecular biology, medicinal chemistry, pharmaceutical science, nutrition, exercise physiology, analytical chemistry, cell biology, pharmacology, clinical medicine, and environmental science.

  • Future Directions in Biocatalysis

    • 2nd Edition
    • Tomoko Matsuda
    • English
    Future Directions in Biocatalysis, Second Edition, presents the future direction and latest research on how to utilize enzymes, i.e., natural catalysts, to make medicines and other necessities for humans. It emphasizes the most important and unique research on biocatalysis instead of simply detailing the ABC’s on the topic. This book is an indispensable tool for new researchers in the field to help identify specific needs, start new projects that address current environmental concerns, and develop techniques based on green technology. It provides invaluable hints and clues for conducting new research on enzymes, with final sections outlining future directions in biocatalysis further expanding the science into new applications.

  • NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes

    • 1st Edition
    • Volume 590
    • English
    Nanoarmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes, Volume 590 is the latest volume in the Methods in Enzymology series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated information on a variety of topics, including specific chapters on Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One, Enzyme Adsorption on Nanoparticle Surfaces Probed by Highly Sensitive Second Harmonic Light Scattering, Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method, and Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts. Users will find this to be an all-encompassing resource on nanoarmoring in enzymes.

  • Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

    • 1st Edition
    • Volume 583
    • English
    Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

  • Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods

    • 1st Edition
    • Volume 582
    • English
    Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.

  • Peptide, Protein and Enzyme Design

    • 1st Edition
    • Volume 580
    • English
    De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction.

  • Rational Design of Enzyme-Nanomaterials

    • 1st Edition
    • Volume 571
    • English
    Rational Design of Enzyme-Nanomaterials... the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in rational design of enzyme-nanomaterials... and includes sections on such topics as conjugation of enzymes and dextran-aldehyde polymers, improved activity of enzymes bound to titanate nanosheet, nano-layered 'stable-on-the-table... biocatalysts and nanoparticle-based enzyme sensors.

  • The Scientist's Guide to Cardiac Metabolism

    • 1st Edition
    • Michael Schwarzer + 1 more
    • English
    The Scientists Guide to Cardiac Metabolism combines the basic concepts of substrate metabolism, regulation, and interaction within the cell and the organism to provide a comprehensive introduction into the basics of cardiac metabolism. This important reference is the perfect tool for newcomers in cardiac metabolism, providing a basic understanding of the metabolic processes and enabling the newcomer to immediately communicate with the expert as substrate/energy metabolism becomes part of projects. The book is written by established experts in the field, bringing together all the concepts of cardiac metabolism, its regulation, and the impact of disease.

  • Cytochrome P450 Function and Pharmacological Roles in Inflammation and Cancer

    • 1st Edition
    • Volume 74
    • English
    Cytochrome P450 Function and Pharmacological Roles in Inflammation and Cancer, the latest volume in the Advances in Pharmacology series, presents not only the function of cytochrome P450 but also its pharmacological roles in inflammation and cancer.

  • Enzyme Nanoparticles

    Preparation, Characterisation, Properties and Applications
    • 1st Edition
    • Chandra S. Pundir
    • English
    This book is the first book in English on nanotechnology and nanomaterials integrating with enzymatic systems, with a focus on nanoparticles and biological applications. It covers comprehensively the relevant topics to understand the development of enzyme nanoparticles as it relates to the complicated structures of enzyme nanoparticles and their functionalization and immobilization on to various supports. The preparation of enzyme nanoparticles, their kinetic properties and applications after immobilization of the immobilized enzyme nanoparticles is described. The use of colour images in all formats of the book will improve the understanding of the topics covered. The book offers an integration of Enzymology and Nanotechnology and provides the latest information on preparation of enzyme nanoparticles, their characterization, their functionalization and immobilization on to various supports and thereafter their kinetic properties and applications in various industries with special reference to Biosensor Technology.

  • Pulp and Paper Industry

    Chemicals
    • 1st Edition
    • Pratima Bajpai
    • English
    Pulp and Paper Industry: Chemicals features in-depth and thorough coverage of Chemical additives in the Pulp and Paper Industry. It discusses use of Enzymes "Green Chemicals" that can improve operations in pulp and paper, describes Chemicals demanded by the end user and many key and niche players such as Akzo Nobel NV, Eka Chemicals AB, Ashland, Inc., BASF, Buckman Laboratories International, Inc., Clariant, Cytec Industries, Inc., Enzymatic Deinking Technologies, LLC, ERCO Worldwide, FMC Corporation, Georgia-Pacific Corporation, Georgia-Pacific Chemicals LLC, Imerys SA, Momentive Specialty Chemicals, Inc., Novozymes, Kemira Chemicals, Nalco Holding Company, Omya AG, Solvay AG, and Solvay Chemicals, Inc.. Paper and pulp processing and additive chemicals are an integral part of the total papermaking process from pulp slurry, through sheet formation, to effluent disposal. Environmental concerns, increased use of recycled waste paper as a replacement for virgin pulp, changes in bleaching and pulping processes, increased efficiency requirements for the papermaking process, limits on effluent discharge as well as international competitiveness have greatly impacted the paper and pulp chemical additive market. This book features in-depth and thorough coverage of Chemical additives in Pulp and Paper Industry.