Skip to main content
Elsevier

Books in Enzymology

  • Bio-Click Chemistry

    Expanding Horizons Beyond the Click
    • 1st Edition
    • Flavia Zacconi + 2 more
    • English
    Bio-Click Chemistry: Expanding Horizons Beyond the Click delves into the dynamic intersection of biocatalysis and click chemistry, providing a comprehensive exploration of these rapidly evolving fields. This book offers in-depth insights into both historical perspectives and the latest advancements, ensuring readers gain a thorough understanding of fundamental principles, practical applications, and future directions. Readers will discover the versatile applications of biocatalysis and click chemistry in pharmaceuticals, biotechnology, biology, biomedicine, nanomedicine, and materials science, equipping researchers, educators, and industry professionals with the tools and techniques needed to foster innovation and real-world impact. Furthermore, the book investigates the emerging trends, challenges, and opportunities in biocatalysis and click chemistry, empowering readers to stay ahead of the curve and drive transformative developments in chemical synthesis and enzymatic engineering. This indispensable guide is designed for researchers, educators, and industry professionals dedicated to advancing their work and exploring new frontiers in chemical synthesis and biocatalytic processes.

  • Super-Resolution Nanoscopy for Protein Imaging

    • 1st Edition
    • Varun Vyas
    • English
    Super-Resolution Nanoscopy for Protein Imaging decodes the intricate world of protein visualization using cutting-edge nanoscopic techniques. This comprehensive guide is crafted to equip master students, PhD candidates, and early-stage researchers with essential knowledge and practical insights into advanced protein imaging technologies. The book covers foundational principles, innovative methodologies, and real-world applications, providing a complete resource for mastering the complexities of protein imaging. This book offers a unique compilation of diverse nanoscopic methods, overcoming the limitation of technique-specific texts currently available.By collecting scattered information into a single volume, it delivers an in-depth exploration of imaging techniques, from Atomic Force Microscopy (AFM) to Electron Microscopy (EM), and Single Molecule Fluorescence Imaging. Each section delves into instrumentation, sample preparation, data analysis, and the latest developments, making it an indispensable reference for understanding how to visualize proteins in their natural environments.

  • Enzyme Technology for Environmental Sustainability, Waste to Wealth, and Circular Economy

    • 1st Edition
    • Shrikaant Kulkarni
    • English
    Enzyme Technology for Environmental Sustainability, Waste to Wealth, and Circular Economy offers a comprehensive look at how enzyme technology can drive environmental progress within the framework of biochemistry. Beginning with foundational concepts, the book introduces readers to the science behind enzymes and their practical applications. Each chapter builds upon these basics, illustrating how enzymes catalyze vital processes that contribute to sustainability and the transformation of waste into valuable resources. Through focused case studies and clear explanations, the book demonstrates the potential of enzyme-driven solutions to address urgent environmental challenges and promote a circular economy.Beyond its central focus, the book stands out by delivering pragmatic examples that remain within biochemical boundaries, avoiding broader discussions in public health or environmental science. It is designed as a valuable reference for biochemists, researchers, and industry professionals, emphasizing collaboration and innovation rooted in biochemical expertise. Readers will gain an enriched understanding of enzyme technology’s role in sustainable industrial practices and the future of environmentally responsible solutions.

  • Carrier-Free Enzyme Immobilization

    Fundamentals and Innovations in Biocatalysis
    • 1st Edition
    • Mehdi Mohammadi + 1 more
    • English
    Carrier-Free Enzyme Immobilization: Fundamentals and Innovations in Biocatalysis explores innovative techniques for immobilizing enzymes without the use of inert carriers, highlighting their advantages, applications, and integration into modern biocatalytic processes. The book begins by introducing the concept of enzyme immobilization and the transition from traditional carrier-bound methods to innovative carrier-free techniques. It discusses the fundamental principles behind carrier-free immobilization, including the types of cross-linkers used and the advantages in enhancing enzyme stability and activity. The book also covers various methods for the structural characterization of carrier-free immobilized enzymes.With a strong focus on practical implementation, it examines the applications of carrier-free immobilized enzymes across different industries, highlighting the pros and cons of the method. It explores novel approaches for producing carrier-free immobilized biocatalysts and provides a comparative analysis of carrier-free versus traditional carrier-bound immobilization techniques. The book also addresses the challenges associated with implementing carrier-free immobilization in industrial processes and proposes potential solutions to overcome these obstacles. It is an essential resource for biochemists, chemical engineers, and researchers in biotechnology who are involved in enzyme research and development.

  • Protein Kinase Inhibitors

    The Promise of Natural Products in Cancer Therapeutics
    • 1st Edition
    • Md. Imtaiyaz Hassan + 1 more
    • English
    Protein Kinase Inhibitors: The Promise of Natural Products in Cancer Therapeutics explores a novel therapeutic avenue for cancer treatment through nutraceutical-based kinase inhibitors. The book covers their historical background, outlook, mechanistic pathways, and clinical aspects, highlighting their relevance in cancer therapy. It also examines the potential of polyphenolic derivatives and other natural compounds in inhibiting key kinases involved in major cancer signaling pathways, focusing on their application in clinical trials and marketed drugs. Sections explore the implications of polyphenols in cancer management, novel sources of breast cancer inhibitors, the role of natural products in lymphoma therapy, and the multitarget potential of phytochemicals. Each chapter is designed to provide a thorough understanding of how natural products can be harnessed to develop innovative cancer therapeutics. It will serve as a valuable resource for academics, scientists, young researchers, students, and pharmacists working or interested in kinase biology and cancer therapeutics with its unique perspective on using nature's own arsenal to combat cancer.

  • Cyclodextrin Glucanotransferase

    A Comprehensive Guide from Gene to Applications
    • 1st Edition
    • Kiransinh Rajput
    • English
    Cyclodextrin Glucanotransferase: A Comprehensive Guide from Gene to Applications explores the essential role of cyclodextrin glucanotransferase (CGTase) in starch degradation and its diverse industrial applications. This book serves as a valuable resource for researchers and industry professionals, offering comprehensive insights from the genetic basis of CGTase to its practical applications. The book covers the production of CGTase by different bacterial genera, including Bacillus spp., and their purification strategies. It delves into the structural and functional characteristics of CGTase, highlighting its catalytic mechanisms, substrate specificity, and factors affecting enzyme activity and stability. Additionally, the book explores recombinant CGTases, their properties, and advancements in protein engineering and enzyme immobilization. With a strong focus on practical applications, the book discusses the remarkable properties of cyclodextrins, such as inclusion complex formation, and their wide range of uses in various industries, including pharmaceuticals, textiles, bioremediation, and food. Detailed case studies and real-world examples illustrate how CGTase and cyclodextrins can address specific challenges and drive innovation in these fields.This book is an essential resource for applied science researchers working in the field of starch processing enzymes, providing them with the knowledge to advance their research and development. It also serves as a valuable reference for researchers in the starch processing industries, offering insights into the latest advancements and practical applications of CGTase and cyclodextrins.

  • Microbial Enzymes

    Classification, Biochemistry, Production and Applications
    • 1st Edition
    • Abdel Moneim Elhadi Sulieman
    • English
    Microbial Enzymes: Classification, Biochemistry, Production and Applications explores the multifaceted roles of microbial enzymes, emphasizing their stability, ease of handling, and manipulation, all of which make them invaluable in various industrial processes. With 25 in-depth chapters, the book delves into the biochemistry of enzymes, their various sources, and their importance in biotechnology. It covers modern techniques for microbial enzyme detection and determination, methods of enzyme immobilization, and the application of microbial enzymes in industries such as food, medicine, pharmaceuticals, and textiles. Special sections discuss the biosynthesis of metal nanoparticles by microbial enzymes and the role of microbial toxins in different sectors. The book's chapters are organized in a way that provides detailed insights into the classification of enzymes, the mechanisms of microbial enzymes, and their industrial importance. Topics include bacterial, fungal, and algal enzymes, their applications, and the latest advancements in enzyme technology. Other sections address the challenge of antibiotic resistance and explores innovative methods for enzyme production and molecular engineering.

  • Recent Trends in Enzyme Immobilization

    • 1st Edition
    • Shailendra Kumar Arya + 3 more
    • English
    Recent Trends in Enzyme Immobilization explores the forefront of enzyme technology, providing a comprehensive guide on the latest methodologies and advancements. This book begins by setting the stage with foundational insights into enzyme immobilization, then progresses to discuss innovative techniques and their applications across various industries, including biopharmaceuticals, environmental science, and food processing. Structured to cater to both newcomers and seasoned professionals in biotechnology and biochemical engineering, each chapter delivers in-depth analysis and up-to-date research findings. Readers will find valuable resources in the practical guidance offered for optimizing enzyme stabilization, activity, and reusability in industrial applications. Moreover, the text addresses the theoretical underpinnings that frame current practices and illuminates future directions for research and technological advancements. Designed as an indispensable resource, this book aims to bridge the gap between academic research and practical application. It equips researchers, practitioners, and advanced students with the necessary tools and knowledge to drive innovation in enzyme immobilization, fostering the development of sustainable solutions and novel approaches in their work. With contributions from leading global experts, Recent Trends in Enzyme Immobilization serves as a critical reference that promises to inspire and guide readers through the complexities and potentials of this dynamic field.

  • Enzymatic Production of Oligosaccharides

    From Basic Research to Industrial Production
    • 1st Edition
    • Carlos Vera + 2 more
    • English
    Enzymatic Production of Oligosaccharides: From Basic Research to Industrial Production, a volume in the Foundations and Frontiers of Enzymology series, covers the enzymes used for the extraction, synthesis, or modification of oligosaccharides, the biochemical properties of those enzymes, and potential technological applications. The book is divided into three parts, the first of which shares the fundamentals on the enzymatic synthesis of oligosaccharides. The book then explores the enzymatic production of oligosaccharides, considering a range of different oligosaccharides and production processes. Final sections cover the technological properties of oligosaccharides and potential applications across the chemical, biochemical, biotechnology, and food industries.This is a useful reference for researchers working with oligosaccharides across biochemistry, enzymology, biotechnology, food chemistry and related fields.

  • Peroxidases

    Biochemical Properties, Roles, Mechanism and Applications
    • 1st Edition
    • Pratima Bajpai
    • English
    Peroxidases: Biochemical Properties, Roles, Mechanism and Applications delves into the world of peroxidases, enzymes crucial in various biochemical and industrial processes. This comprehensive guide starts with an exploration of the origins and biochemical properties of peroxidases from sources such as animals, plants, and microbes. It explains their classifications, mechanisms of action, and the molecular evolution of different peroxidase families. Additionally, the book discusses the enhancement of peroxidase activities and their role in biotechnology. The second part of the book highlights the applications of peroxidase in organic synthesis, the pulp-paper industry, diagnostic kits, and biosensors.Finally, the book examines advanced healthcare applications, including targeted cancer therapies and enzyme immunoassays. This detailed resource is aimed at researchers, practitioners, and students in biochemistry, biotechnology, and related scientific fields.

  • Handbook of Proteolytic Enzymes

    Metallopeptidases
    • 4th Edition
    • Neil D. Rawlings + 1 more
    • English
    Handbook of Proteolytic Enzymes, Metallopeptidases has stood as most comprehensive work in the field of applied enzymology and biocatalysis since the first edition published in 1998. Extensively revised and updated, the new fourth edition is an essential reference for biochemists, biotechnologists, and molecular biologists across academia and industry. Edited by world-renowned experts in the field, and with five volumes available for individual sale, this work provides detailed information on all known proteolytic enzymes researched to-date, with expanded coverage of metallopeptidases, cysteine peptidases, serine and threonine peptidases, aspartic and glutamic peptidases, and inhibitors of proteolytic enzymes.This volume includes over 300 chapters on known metallopeptidases enzymes, including their name, history, activity and specificity, structural chemistry, preparation, biological aspects, and distinguishing features, with 2D structures of peptidases in color, extensive references, and links to PubMed and MEROPS databases.

  • Cryptic Enzymes and Moonlighting Proteins

    • 1st Edition
    • Helen Irving + 2 more
    • English
    Cryptic Enzymes and Moonlighting Proteins, a new volume in the Foundations and Frontiers in Enzymology series, offers a thorough overview of cryptic enzymes and moonlighting proteins in signaling cascades. In early chapters, leading international contributors discuss evolutionary considerations for moonlighting proteins, moonlighting interactions in the extracellular matrix, eukaryotic moonlighting proteins, modulating, moonlighting kinases, moonlighting proteins in neurobiology signaling, metabolic enzymes moonlighting as RNA binding and regulatory proteins. Later, methods-driven chapters discuss practical aspects of identifying hidden moonlighting domains in proteins, computational approaches and bioinformatic tools for the identification of cryptic enzymes, establishing cryptic enzyme interactomes, and assessing contributions of moonlighting proteins to signal cascades.The book also explores recent advances in research and brings together an array of information across different fields to enable better targeting of these exciting proteins and their interactomes. With a clear focus on the role of moonlighting and cryptic enzymes in signal transduction, users will find examples of cryptic enzymes across species, as well as those in human healthy biology and pathogenesis.

  • Whole Cell Biocatalysis

    Fundamentals and Applications
    • 1st Edition
    • Sergio Huerta-Ochoa + 2 more
    • English
    Whole Cell Biocatalysis, a volume in the Foundations and Frontiers of Enzymology series, offers a detailed overview of the process of biocatalysis using whole cells as an alternative to enzyme biocatalysis. The book examines the potential applications and advantages of whole cell biocatalysis, including its use in the production of fine chemicals, renewable energy, and drug discovery and development. Whole cell biocatalysis for large scale production and non-conventional media are also covered. In addition, the latest methods and techniques are investigated, including cell immobilization, permeabilization, synthetic biology, computational metabolic engineering, and molecular genetics.This book provides a comprehensive summary on whole cell biocatalysis and the latest developments in this emerging field. It is an invaluable reference for researchers working across biochemistry, enzymology, biotechnology, and related fields.

  • Mitochondrial Transplantation and Transfer

    Biology, Methods, Applications, and Disease
    • 1st Edition
    • Gokhan Burcin Kubat + 2 more
    • English
    Mitochondrial Transplantation and Transfer: Biology, Methods, Applications, and Disease, a volume in the Translational and Applied Bioenergetics series, offers a detailed overview of mitochondrial transplantation across fundamental biology, disease and early therapeutics research. Here, more than twenty experts in the field discuss transplantation and transfer of healthy mitochondria and define future steps to improve new therapeutic applications across a variety of diseases. Emphasis is placed on the physiology of mitochondrial transfer, isolation processes and their applications, transfer methods, and diagnostic and treatment use across cardiovascular, metabolic, and neurological diseases, among other disease types. Step-by-step instruction is provided around research protocols and methods discussed, along with careful attention to the ethical implications of mitochondrial transplantation research and application.

  • Biocatalysis in Asymmetric Synthesis

    • 1st Edition
    • Gonzalo De Gonzalo + 1 more
    • English
    Biocatalysis in Asymmetric Synthesis, a new volume in the Foundations and Frontiers of Enzymology series, offers an applied discussion on synthesizing biological catalysts using asymmetric synthesis for varying applications. Here, global experts in the field analyze a wide variety of biocatalysts and their physical states, process conditions for their asymmetric synthesis, solvents required during synthesis, and even downstream procedures for the recovery of final products. The book adopts an interdisciplinary approach, merging fundamental biology and its synthetic applications across industries with a wide range of practical examples, from directed evolution to biotransformation and production of novel enzymes and non-conventional catalysts. Throughout the book, the impact and application of biocatalysis in sustainable processing is considered in-depth. This book will also help non-experts in biocatalysis to apply this knowledge in their own research, providing a thorough overview of the ways asymmetric biocatalytic approaches may be adapted for different disciplines and downstream products.

  • Translational Glycobiology in Human Health and Disease

    • 1st Edition
    • Michelle Kilcoyne + 1 more
    • English
    Along with nucleic acids, proteins, and lipids, carbohydrates stand as one of four main components of cellular architecture. However, glycobiology (or carbohydrate bioscience) is little understood by non-experts, partly because carbohydrates are a complex, diverse class of molecules structurally and functionally. In recent years, advances in computational analytics (glycomics) have allowed us to better interpret and realize the importance of glycobiology in human health and disease, and glycans and their associated processes have been shown to play a significant role across a variety of disease types. As the biomedical sciences continue to adopt multi-omic and precision medicine approaches, a greater understanding of glycobiology is essential for maintaining healthy physiology and advancing disease treatment.Translatio... Glycobiology in Human Health and Disease offers a deep examination of glycobiology for experts and non-experts alike in areas ranging from the role of glycobiology in chronic and infectious diseases to advances in technologies for higher throughput analysis and diagnosis. While keeping human health in the forefront, this book integrates a thorough discussion of glycobiology fundamentals with its growing areas of application and societal impact. With emphasis throughout on the interdisciplinary nature of glycosciences, this book also features perspectives from the health, computational (glycoanalytics), materials, biopharmaceutical, and diagnostic sciences.Disease and speciality areas addressed include gycoimmunology, neuroglycobiology, commensal glycobiology, gut health, regenerative medicine and glycobiology, glycobiology and cancer, congenital disorders of glycosylation, infectious disease glycobiology, and parasite glycobiology. Computational approaches discussed, supporting the advance of new research, include advanced glycoanalytics, glycomics microarrays, glycoengineering, and glycol systems biology. Additionally, authors consider impact areas for society and public health, such as glycobiology and entrepreneurship, policy and regulatory requirements for glycosylation, future research, and translation to new diagnostics and drug discovery.

  • Cyanobacteria

    Metabolisms to Molecules
    • 1st Edition
    • Arun Kumar Mishra + 1 more
    • English
    Cyanobacteria: Metabolisms to Molecules covers diversity, fundamental metabolisms, crucial metabolities, their synthesis, and bioinformatics topics surrounding cyanobacteria, an organism regarded as the most important organism to sustain life on the planet during ancient times. Cyanobacteria are among the first pioneering communities on various harsh habitat, hydrarch or xerarch, which finally facilitate the emergence of vast communities including higher plants. Being the progenitor of chloroplast, the cyanobacterial metabolisms has always fascinated microbiologists. Additionally, the ability of these prokaryotes to produce valuable and prolific sources of natural products signified their role in an array of industrial sectors. Therefore, in this book, the book's authors accumulate the current knowledge of cyanobacterial metabolisms and molecules as a valuable asset for students, researchers, and biotechnologists.

  • Metalloenzymes

    From Bench to Bedside
    • 1st Edition
    • Claudiu T. Supuran + 1 more
    • English
    Metalloenzymes: From Bench to Bedside offers a thorough overview of metalloenzymes, spanning biochemical and structural features, pharmacology, and biotechnological applications. After a brief overview, international experts in the field discuss a wide range of magnesium, calcium, zinc, manganese, nickel, iron, copper, cadmium, molybdenum, and tungsten enzymes, along with catalytic roles within their active sites. With a uniform approach throughout, each chapter includes the structure and function of the enzyme, physiologic and pathologic roles, inhibitors and activators of the enzyme (and their design), and clinical agents or compounds applied in medicine and drug discovery. This book enables scientists across academia and industry to adopt ongoing metalloenzyme research, and continuous discovery of novel metalloenzymes, in new life science studies and clinical applications.

  • Polysaccharide Degrading Biocatalysts

    • 1st Edition
    • Rosana Goldbeck + 1 more
    • English
    The transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis. Polysaccharide-Degra... Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrad... enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.

  • Developments and Applications of Enzymes From Thermophilic Microorganisms

    • 1st Edition
    • Pratima Bajpai
    • English
    Developments and Applications of Enzymes from Thermophilic Microorganisms extensively presents the industrial application of thermophilic/hyperth... enzymes. The book brings thorough and in-depth coverage on the role of these enzymes in a broad range of industries, focusing on present scenarios of these enzymes in biofuel industries, including recent advancements. The use of thermophilic enzymes in 2G biorefineries may enable the whole production process to take place at high temperatures, allowing increased reaction rate and reduced costs. Researchers in biochemistry, microbiology, microbial technology, biotechnology, molecular biology and bioresource technology will benefit from the new insights given on potential applications of hyperthermophiles. Hyperthermophilic enzymes, many of which survive at temperatures at or above 100C, contain novel macromolecules and metabolic systems which represent a vast resource for fundamental molecular and physiological studies, and for potential exploitation in biotechnology.

  • Structure and Intrinsic Disorder in Enzymology

    • 1st Edition
    • Munishwar Nath Gupta + 1 more
    • English
    Structure and Intrinsic Disorder in Enzymology offers a direct, yet comprehensive presentation of the fundamental concepts, characteristics and functions of intrinsically disordered enzymes, along with valuable notes and technical insights powering new research in this emerging field. Here, more than twenty international experts examine protein flexibility and cryo-enzymology, hierarchies of intrinsic disorder, methods for measurement of disorder in proteins, bioinformatics tools for predictions of structure, disorder and function, protein promiscuity, protein moonlighting, globular enzymes, intrinsic disorder and allosteric regulation, protein crowding, intrinsic disorder in post-translational, and much more. Chapters also review methods for study, as well as evolving technology to support new research across academic, industrial and pharmaceutical labs.

  • Biocatalyst Immobilization

    Foundations and Applications
    • 1st Edition
    • Maria Lujan Ferreira
    • English
    Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

  • Biocatalysis in Green Solvents

    • 1st Edition
    • Pedro Lozano
    • English
    Biocatalysis in Green Solvents offers a pragmatic overview and instruction in biocatalysis and enzymology of green solvents for sustainable industries and medicine, running from concept to application. Here, international experts in the field discuss structure-function relationships of enzymes in ionic liquids (ILs) and examine how enzymes act as selective catalysts for fine biochemical synthesis in non-aqueous environments. Several integral green biochemical processes of biocatalytic transformation and pure product separation are described in detail. Application focused chapters discuss the role of biocatalysis in creating and implementing deep eutectic solvents, biomass derived solvents, sub and supercritical fluids, carbon dioxide biphasic systems, and enzymatic membrane reactors, as well as applying these biocatalytic processes in drug discovery and production.

  • Microbial Xylanolytic Enzymes

    • 1st Edition
    • Pratima Bajpai
    • English
    Microbial Xylanolytic Enzymes describes the enzyme structure and its interaction with plant cell walls, the properties and production of different enzymes and their applications, and the knowledge gathered on the hydrolysis mechanism of hemicellulose. The knowledge gathered about the hydrolysis mechanism of the hemicelluloses, especially xylans, has greatly promoted the rapid application of these enzymes in new areas. In recent years, there has been a spurt of interest in xylan degrading enzymes due to their applications in several industrial processes, including paper and pulp industries, food and feed industries, biofuel industry, textile industry, chemical and pharmaceutical industry, brewing industry, and more. Xylan is the principal type of hemicellulose. An enzymatic complex is responsible for the hydrolysis of xylan, but the main enzymes involved are enzymes produced by fungi, bacteria, yeast, algae, protozoans, and more.

  • Redox Chemistry and Biology of Thiols

    • 1st Edition
    • Beatriz Alvarez + 3 more
    • English
    Redox Chemistry and Biology of Thiols offers an applied, comprehensive overview of redox chemistry and biology of thiol-dependent processes. Running from basic biology and chemistry of redox phenomena to research methods and highlighting recently identified roles of thiols across cellular and bodily systems, this book draws upon a range of disciplines to illuminate new research directions, new applications of thiol studies, and clinical translation. Sections cover thiol oxidizing species, thiol reactivity and modifications, thioredoxin, glutaredoxin, glutathione, peroxidases, thiol repair enzymes, thiol oxidative signaling, disulfide bond formation, thiol-based redox biosensors, cysteine and hydrogen sulfide metabolism, iron-sulfur cluster biogenesis, thiols in chloroplasts, blood thiols, sugar and polyamine thiols in pathogenic organisms, redox medicine (therapeutic applications of thiols and drug development), as well as methods and bioinformatics tools.

  • Protein Kinase Inhibitors

    From Discovery to Therapeutics
    • 1st Edition
    • Md. Imtaiyaz Hassan + 1 more
    • English
    Protein Kinase Inhibitors: From Discovery to Therapeutics offers a foundational, pragmatic overview of protein kinases inhibitors and their potential role in disease modulation and treatment. Here, international experts in the field offer an integrated discussion of kinase inhibitor biology, biomarker discovery, and methods for drug design and development. After a brief overview of kinases and kinase inhibitors, subsequent chapters discuss individual kinases that are representative of the wider kinases and kinase families, including their roles in disease pathogenesis, underlying mechanisms, potential inhibitors and their modes of action for therapeutic modulation. Several potential drugs under different stages of clinical trials are discussed, including their relevance to cancer, diabetes, obesity, cardiovascular, neurological, and auto-immune and inflammatory disease, among other disorders. The book also addresses the challenges and opportunities for future kinase inhibitor development.

  • Cholesterol

    From Chemistry and Biophysics to the Clinic
    • 1st Edition
    • Anna N. Bukiya + 1 more
    • English
    With Cholesterol, Drs. Anna Bukiya and Alex Dopico have compiled a comprehensive resource on biological and clinical aspects of cholesterol, spanning biophysics and biochemistry, as well as the latest pharmacological discoveries employed to tackle disorders associated with abnormal cholesterol levels. Early chapters on basic biology offer guidance in cholesterol lab chemistry, cholesterol metabolism and synthesis, molecular evolution of cholesterol and sterols, cholesterol peptides, and cholesterol modulation. Chapters on cellular and organismal development discuss cholesterol transport in blood, lipoproteins, and cholesterol metabolism; cholesterol detection in the blood; cellular cholesterol levels; hypercholesterolemia... and the role of cholesterol in early human development. Pathophysical specialists consider familial hypobetalipoproteine... critical illness and cholesterol levels, coronary artery disease, CESD, cholesterol and viral pathology, cholesterol and neurodegenerative disorders, and cholesterol and substance use disorders. A final section examines pharmacology of drug delivery systems targeting cholesterol related disorders, cholesterol receptors, cholesterol reduction, statins, citrate lyase, cyclodextrins, and clinical management. Cholesterol: From Biophysics and Biochemistry to Pathology and Pharmacology empowers researchers, students, and clinicians across various disciplines to advance new cholesterol-based studies, improve clinical management, and drive drug discovery.

  • RNA Therapeutics

    The Evolving Landscape of RNA Therapeutics
    • 1st Edition
    • Paloma H. Giangrande + 2 more
    • English
    RNA Therapeutics: The Evolving Landscape of RNA Therapeutics provides a comprehensive overview of RNA therapeutic modalities, from bench-to-bedside, with an emphasis on the increasingly impactful areas of gene therapy, oligonucleotide therapeutics, gene editing and delivery. International leaders in the field examine RNA-based therapeutics tools that have been developed to-date to modulate cellular processes such as transcription, translation and protein function. Approved RNA-based therapies and lessons learned from failed therapies are discussed in-depth, as are evolving advances in RNA biochemical analysis, and similar advances that are enabling clinical application of RNA-based therapies. Later sections discuss delivery technologies, remaining hurdles in research and translation, the therapy development process from the lab to the clinic, and novel RNA-based therapies currently in development.

  • Metabolomics Perspectives

    From Theory to Practical Application
    • 1st Edition
    • Jacopo Troisi
    • English
    Metabolomics Perspectives: From Theory to Practical Application is an expertly written volume, which provides a thorough description of the current state-of-the-art in the metabolomics field. The philosophy behind the book is to guide the reader in a step-by-step exploration of metabolomics experiments, ranging from sample preparation to data extraction, analysis and interpretation, and to discuss the main current applications and future perspectives of this emerging science. Armed with critical insights, coupled with a clear writing, the book consists of three main sections. The first one introduces the pivotal theoretical fundamentals and provides a comprehensive overview of the "wet" laboratory workflow, including protocol instructions and a detailed description of experimental methods and analytical techniques. The second section covers a wide range of topics in the context of data analysis, including guidance in exploratory analysis, supervised and unsupervised machine learning approaches and validation and optimization methods. In addition to the several examples reported in the text, the book features an R package, specifically designed to perform all the described algorithms, which is hosted on a companion website (www.metabolomicsper... together with several sets of available metabolomic data. Finally, an extensive dissertation describes the latest advances and the major fields of interest for metabolomics applications, highlighting their crucial potentials for future biomedical research. Thus, this book represents a must-read for both experienced researchers, interested in metabolomics, and newcomers to the field.

  • Proteolytic Signaling in Health and Disease

    • 1st Edition
    • Andre Zelanis
    • English
    In recent years, powered by evolving technologies and experimental design, studies have better illuminated the regulating role of proteolytic enzymes across human development and pathologies. Proteolytic Signaling in Health and Disease provides an in-depth discussion of fundamental physiological and developmental processes regulated by proteases, from protein turnover and autophagy to antigen processing and presentation and major histocompatibility complex (MHC) molecules. Moving on from basic biology, international chapter authors examine a range of pathological conditions associated with proteolysis, including inflammation, wound healing, and cancer. Later chapters discuss the newly discovered network of connected events among proteases (and their inhibitors), the so-called ‘protease web’, and how best to study it. This book also empowers new research with up-to-date analytical methods and step-by-step protocols for studying proteolytic signaling events.

  • Polymeric Supports for Enzyme Immobilization

    Opportunities and Applications
    • 1st Edition
    • Alka Dwevedi
    • English
    Enzyme immobilization has been approached for finding solutions for various critical problems associated with industries, medicine, environment, agriculture, etc. Especially since last decades, several innovative researches have come up to look for enhancing catalytic efficiency, reusability of immobilized enzyme and longer stability by introducing range of immobilizing supports, supports modifiers through introduction of several chemical agents (non-toxic) and adopting innovative enzyme immobilization methods. In the present book, polymeric supports have been focussed for enzyme immobilization, especially due to their versatility in immobilizing different enzymes for different large scale enzyme reactors to be used for several applications. Especially, polymers can be modified according to applications and enzyme properties which have made it supports of choice for all several enzyme based applications. Polymeric Supports for Enzyme Immobilization: Opportunities and Applications offers in-depth discussions of known polymeric enzyme support materials, reaction processes, and optimized methods to enhance enzyme immobilization. Case-based chapters examine methods of enzyme immobilization onto various polymeric supports, their surface chemistry and physical morphology followed by implementation of polymers based immobilized enzymes in various applications, viz. medicine, environment, industries, clean energy, disease diagnosis, drug delivery etc. This book has prime focus to allow several researchers across the world to provide updated technological details and incite to contribute more innovative work in coming years to find solutions to several critical problems.

  • Biologically Active Peptides

    From Basic Science to Applications for Human Health
    • 1st Edition
    • Fidel Toldra + 1 more
    • English
    Biologically Active Peptides: From Basic Science to Applications for Human Health stands as a comprehensive resource on bioactive peptide science and applications. With contributions from more than thirty global experts, topics discussed include bioactive peptide science, structure-activity relationships, best practices for their study and production, and their applications. In the interdisciplinary field of bioactive peptides, this book bridges the gap between basic peptide chemistry and human physiology, while reviewing recent advances in peptide analysis and characterization. Methods and technology-driven chapters offer step-by-step guidance in peptide preparation from different source materials, bioactivity assays, analysis and identification of bioactive peptides, encoding bioactive peptides. Later, applications across disease areas and medical specialties are examined in-depth, including the use of bioactive peptides in treating obesity, diabetes, osteoporosis, mental health disorders, food allergies, and joint health, among other disorders, as well as bioactive peptides for sensory enhancement, sports and clinical nutrition, lowering cholesterol, improving cardiovascular health, and driving advances in biotechnology.

  • Clinical Bioenergetics

    From Pathophysiology to Clinical Translation
    • 1st Edition
    • Sergej M. Ostojic
    • English
    Clinical Bioenergetics: From Pathophysiology to Clinical Translation provides recent developments surrounding the etiology and pathophysiology of inherited and acquired energy-delated disorders. Across 40 chapters, world leaders in bioenergetics and mitochondrial medicine discuss novel methodologies designed to identify deficiencies in cellular bioenergetics, as well as the safety and efficacy of emerging management strategies to address poor cellular bioenergetics. Topics discussed include the omics landscape of impaired mitochondrial bioenergetics, hormones, tissue bioenergetics and metabolism in humans. Disease-specific case studies, modes of analysis in clinical bioenergetics, and therapeutic opportunities for impaired bioenergetics, addressing both known treatment pathways and future directions for research, are discussed in-depth. Diseases and Disorders examined include brain injury, chronic fatigue syndrome, psychiatric disorders, pulmonary fibrosis, neurodegenerative disorders, heart failure, chronic kidney disease, obesity, and insulin resistance, among others.

  • Protocols in Biochemistry and Clinical Biochemistry

    • 1st Edition
    • Shweta Pandey + 2 more
    • English
    Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.

  • Advanced Methods in Molecular Biology and Biotechnology

    A Practical Lab Manual
    • 1st Edition
    • Khalid Z. Masoodi + 2 more
    • English
    Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

  • Protein Biosynthesis Interference in Disease

    • 1st Edition
    • Elena L. Paley
    • English
    Protein Biosynthesis Interference in Disease offers a thorough discussion and overview of protein biosynthesis interference, its mechanisms of action, and influence over disease processes. This book examines the role of protein biosynthesis interference in Alzheimer’s and other neurodegenerative conditions, cancer and inflammatory disorders, with specific attention paid to the biochemical dynamics of tryptamine, biogenic amines and aminoacyl-tRNA synthetases in these pathologies. Methods of regulating protein translation and interference mechanisms, including gene therapy, are presented, empowering biochemists, molecular biologists, disease researchers, and health professionals to understand the underlying factors of protein disease and improve patient outcomes.

  • Lipid Signaling and Metabolism

    • 1st Edition
    • James M. Ntambi
    • English
    Lipid Signaling and Metabolism provides foundational knowledge and methods to examine lipid metabolism and bioactive lipid signaling mediators that regulate a broad spectrum of biological processes and disease states. Here, world-renowned investigators offer a basic examination of general lipid, metabolism, intracellular lipid storage and utilization that is followed by an in-depth discussion of lipid signaling and metabolism across disease areas, including obesity, diabetes, fatty liver disease, inflammation, cancer, cardiovascular disease and mood-related disorders. Throughout, authors demonstrate how expanding our understanding of lipid mediators in metabolism and signaling enables opportunities for novel therapeutics. Emphasis is placed on bioactive lipid metabolism and research that has been impacted by new technologies and their new potential to transform precision medicine.

  • The Human Mitochondrial Genome

    From Basic Biology to Disease
    • 1st Edition
    • Giuseppe Gasparre + 1 more
    • English
    The Human Mitochondrial Genome: From Basic Biology to Disease offers a comprehensive, up-to-date examination of human mitochondrial genomics, connecting basic research to translational medicine across a range of disease types. Here, international experts discuss the essential biology of human mitochondrial DNA (mtDNA), including its maintenance, repair, segregation, and heredity. Furthermore, mtDNA evolution and exploitation, mutations, methods, and models for functional studies of mtDNA are dealt with. Disease discussion is accompanied by approaches for treatment strategies, with disease areas discussed including cancer, neurodegenerative, age-related, mtDNA depletion, deletion, and point mutation diseases. Nucleosides supplementation, mitoTALENs, and mitoZNF nucleases are among the therapeutic approaches examined in-depth. With increasing funding for mtDNA studies, many clinicians and clinician scientists are turning their attention to mtDNA disease association. This book provides the tools and background knowledge required to perform new, impactful research in this exciting space, from distinguishing a haplogroup-defining variant or disease-related mutation to exploring emerging therapeutic pathways.

  • Vibrational Spectroscopy in Protein Research

    From Purified Proteins to Aggregates and Assemblies
    • 1st Edition
    • Yukihiro Ozaki + 3 more
    • English
    Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.

  • Mitochondrial Disease Genes Compendium

    From Genes to Clinical Manifestations
    • 1st Edition
    • Marni J. Falk
    • English
    The field of Mitochondrial Medicine has been dominated by symptom constellation-based diagnostic categorization since the first clinical syndrome was described three decades ago. Now, as rapidly expanding knowledge has revealed that mitochondrial diseases may result from several hundred distinct gene disorders with extensive clinical and mutation heterogeneity, the most useful guide for clinical care and research embraces a gene-centric approach to each individual’s disorder. Together with international colleagues, Dr. Marni Falk has developed the Mitochondrial Disease Sequence Data Resource (MSeqDR), an online, community curated, centralized data resource of mitochondrial disease data from a genomic perspective. Here, in the Mitochondrial Disease Genes Compendium, Dr. Marni Falk and a team of international experts have built off their work on MSeqDR to provide an all-in-one, readily accessible, and easy-to-use at point of care reference on 256 mitochondrial disease genes from a gene-based perspective. In this book, clinicians and researchers will find a complete overview of mitochondrial disease genes relevant across all specialties, cataloging and building context around clinical features and the genetic basis of each condition. Within, each "gene page" offers an in-depth, referenced view of the relevant clinical disease spectrum, including gene and protein descriptions, year discovered, inheritance pattern(s), age ranges affected, major clinical features and severity range, clinical pearls, known therapies, available support groups, animal models, and gene-specific basic, translational, or clinical research activities now underway. Links provided on each gene page direct readers to MSeqDR for new findings, up-to-date genomic variant data, and user friendly informatics tools accessible to general clinicians and sophisticated geneticists or bioinformaticians alike, ensuring access to updated information on each condition.

  • Protein Homeostasis Diseases

    Mechanisms and Novel Therapies
    • 1st Edition
    • Angel L. Pey
    • English
    Protein Homeostasis Diseases: Mechanisms and Novel Therapies offers an interdisciplinary examination of the fundamental aspects, biochemistry and molecular biology of protein homeostasis disease, including the use of natural and pharmacological small molecules to treat common and rare protein homeostasis disorders. Contributions from international experts discuss the biochemical and genetic components of protein homeostasis disorders, the mechanisms by which genetic variants may cause loss-of-function and gain-of-toxic-functi... and how natural ligands can restore protein function and homeostasis in genetic diseases. Applied chapters provide guidance on employing high throughput sequencing and screening methodologies to develop pharmacological chaperones and repurpose approved drugs to treat protein homeostasis disorders.

  • Oxidative Stress

    Eustress and Distress
    • 1st Edition
    • Helmut Sies
    • English
    Oxidative Stress: Eustress and Distress presents current knowledge on oxidative stress within the framework of redox biology and translational medicine. It describes eustress and distress in molecular terms and with novel imaging and chemogenetic approaches in four sections: A conceptual framework for studying oxidative stress. Processes and oxidative stress responses. Signaling in major enzyme systems (oxidative eustress), and damaging modification of biomolecules (oxidative distress). The exposome addresses lifelong exposure and impact on health, nutrient sensing, exercise and environmental pollution. Health and disease processes, including ischemia-reperfusion injury, developmental and psychological disorders, hepatic encephalopathy, skeletal muscle disorders, pulmonary disease, gut disease, organ fibrosis, and cancer. Oxidative Stress: Eustress and Distress is an informative resource useful for active researchers and students in biochemistry, molecular biology, medicinal chemistry, pharmaceutical science, nutrition, exercise physiology, analytical chemistry, cell biology, pharmacology, clinical medicine, and environmental science.

  • Carbonic Anhydrases

    Biochemistry and Pharmacology of an Evergreen Pharmaceutical Target
    • 1st Edition
    • Claudiu T. Supuran + 1 more
    • English
    Carbonic Anhydrases provides an interdisciplinary review of the burgeoning carbonic anhydrase (CA) research area, spanning from CAs classification (biochemical and structural features) to drug design and pharmacology of CA inhibitors and activators, finally touching on the biotechnological applications of these metalloenzymes. The book adopts a clear step-by-step approach and introduction to this intricate and highly interdisciplinary field. A diverse range of chapters from international experts speak to CA classification and distribution, the mechanisms of action and drug design of inhibitors and activators, the druggability of the various isoforms in the treatment of a multitude of diseases, and threats to human health. Carbonic Anhydrases provides biology, biochemistry, and medicinal chemistry students and researchers a thorough discussion and update on the evergreen and expanding research area of CAs.

  • Future Directions in Biocatalysis

    • 2nd Edition
    • Tomoko Matsuda
    • English
    Future Directions in Biocatalysis, Second Edition, presents the future direction and latest research on how to utilize enzymes, i.e., natural catalysts, to make medicines and other necessities for humans. It emphasizes the most important and unique research on biocatalysis instead of simply detailing the ABC’s on the topic. This book is an indispensable tool for new researchers in the field to help identify specific needs, start new projects that address current environmental concerns, and develop techniques based on green technology. It provides invaluable hints and clues for conducting new research on enzymes, with final sections outlining future directions in biocatalysis further expanding the science into new applications.

  • Matrix Metalloproteinases and Tissue Remodeling in Health and Disease: Target Tissues and Therapy

    • 1st Edition
    • Volume 148
    • English
    Matrix Metalloproteinases and Tissue Remodeling in Health and Disease: Target Tissues and Therapy, Volume, Volume 148, the latest volume in the Progress in Molecular Biology and Translational Science series covers a variety of timely topics, with chapters focusing on The Role of Matrix Metalloproteinases in Development, Repair, and Destruction of the Lungs, Matrix Metalloproteinases in Kidney Disease: Role in Pathogenesis and Potential as a Therapeutic Target, Regulation of Matrix Metalloproteinase in the Pathogenesis of Diabetic Retinopathy, Matrix Metalloproteinases in Normal Pregnancy and Preeclampsia, and Matrix Metalloproteinases, Neural Extracellular Matrix, and Central Nervous System Pathology. This volume is the second part of a thematic on matrix metalloproteinases and tissue remodeling in health and disease. It focuses on the role of MMPs in other systems, target tissues, and pathological disorders and the potential benefits of MMP inhibitors in various disorders.

  • DNA Repair Enzymes: Cell, Molecular, and Chemical Biology

    • 1st Edition
    • Volume 591
    • English
    DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.

  • NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes

    • 1st Edition
    • Volume 590
    • English
    Nanoarmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes, Volume 590 is the latest volume in the Methods in Enzymology series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated information on a variety of topics, including specific chapters on Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One, Enzyme Adsorption on Nanoparticle Surfaces Probed by Highly Sensitive Second Harmonic Light Scattering, Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method, and Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts. Users will find this to be an all-encompassing resource on nanoarmoring in enzymes.

  • Enzymes as Sensors

    • 1st Edition
    • Volume 589
    • English
    Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring. This state-of-the-art series is ideal for those interested in the latest information on enzymology, with this edition focusing on sensors and their role in enzymes.

  • Enzymology at the Membrane Interface: Intramembrane Proteases

    • 1st Edition
    • Volume 584
    • English
    Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

  • Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

    • 1st Edition
    • Volume 583
    • English
    Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.