Mitochondrial Transplantation and Transfer
Biology, Methods, Applications, and Disease
- 1st Edition - August 3, 2024
- Imprint: Academic Press
- Editors: Gokhan Burcin Kubat, Oner Ulger, Serdar Gunaydin
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 1 8 8 5 8 - 9
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 8 8 5 9 - 6
Mitochondrial Transplantation and Transfer: Biology, Methods, Applications, and Disease, a volume in the Translational and Applied Bioenergetics series, offers a detailed overvie… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteMitochondrial Transplantation and Transfer: Biology, Methods, Applications, and Disease, a volume in the Translational and Applied Bioenergetics series, offers a detailed overview of mitochondrial transplantation across fundamental biology, disease and early therapeutics research. Here, more than twenty experts in the field discuss transplantation and transfer of healthy mitochondria and define future steps to improve new therapeutic applications across a variety of diseases. Emphasis is placed on the physiology of mitochondrial transfer, isolation processes and their applications, transfer methods, and diagnostic and treatment use across cardiovascular, metabolic, and neurological diseases, among other disease types.
Step-by-step instruction is provided around research protocols and methods discussed, along with careful attention to the ethical implications of mitochondrial transplantation research and application.
- Provides an in-depth discussion of mitochondrial transplantation, considering fundamental biology, disease implications, research methods and applications
- Discusses recent advances in the field and their meaning for future research and clinical utility
- Considers ethical dimensions of mitochondrial transplantation research and use in therapeutics
- Examines mitochondrial transplantation across a range of disease types, from neurological to cardiovascular disease and ischemia
Active researchers in biochemistry, molecular biology, cell biology, oncology, and pharmacology, Students and Clinicians
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Contributors
- Preface
- Acknowledgments
- Section I. Introduction
- Chapter 1. Mitochondrial transplantation, methods and mitochondrial dysfunction related diseases: An overview
- Chapter 2. Mitochondria and cell metabolism
- Introduction
- Mitochondrial structure
- Powerhouse of the cell
- Tricarboxylic acid cycle (Krebs cycle–citric acid cycle)
- Electron transport chain
- Biosynthesis of reactive oxygen species and mitochondria
- Chapter 3. Mitochondrial dynamics and mitochondrial biogenesis
- Mitochondrial functions
- Mitochondrial biogenesis
- Mitochondrial dynamics
- Different cells and mitochondrial dynamics
- Conclusion
- Chapter 4. Mitochondrial DNA and heteroplasmy
- Replication, transcription, and translation of mtDNA
- Inheritance of mitochondrial DNA
- Mitochondrial homoplasmy and heteroplasmy
- Dynamics of mitochondrial DNA inheritance
- Threshold effect
- Genetic bottleneck
- Heterogeneity
- Point mutations
- Deletion mutations
- Epigenetics
- Mitochondrial damage checkpoint (mitocheckpoint)
- Exploring mitochondrial genome—in silico interpretation of mtDNA
- Conclusion
- Section II. Physiological mitochondrial transfer
- Chapter 5. Tunneling nanotubes
- Tunneling nanotubes
- TNTs morphology and structure
- Biogenesis and formation of the TNT
- TNT modulation
- TNT-mediated transfer
- Nucleic acid transfer
- Mitochondrial transfer
- Roles of TNTs in disease and healthy
- Cancer
- Neurodegenerative disease
- Conclusion
- Chapter 6. The role of extracellular vesicles and gap junctions in inter-cellular mitochondrial transfer
- Introduction
- Extracellular vesicles
- Gap junctions
- Concluding remarks
- Section III. Mitochondrial transplantation from isolation process to application
- Chapter 7. Isolation process for mitochondrial transplantation
- Introduction
- Mitochondria structure and function
- Mitochondrial isolation
- Mitochondrial source
- Isolation methods
- Transplantation methods
- Discussion
- Isolation of intact mitochondria from cultured cells
- Chapter 8. Determination of mitochondrial number and function
- Introduction
- Mitochondrial number and integrity
- Mitochondrial function
- Conclusions
- Flow Cytometry analysis of transmembrane potential in isolated mitochondria stained with JC-1 dye
- Chapter 9. Storage of isolated mitochondria
- Introduction
- Cold storage of mitochondria
- Cryopreservation of isolated mitochondria
- Critical points
- Methods
- Conclusions
- Cryopreservation of mitochondria isolated from rat heart (Nukala et al., 2006)
- Section IV. Application methods and limitations of mitochondrial transplantation
- Chapter 10. Mitochondrial transplantation methods
- Coincubation
- Microinjection
- Penetrating peptides
- Mitoception
- Photothermal nanoblade and BLAST (Biophotonic laser assisted surgery tool)
- Magnetomitotransfer
- MitoPunch
- FluidFM
- EV mediated mitochondrial transplantation/transfer
- Protocol of coincubation method of Suvay et al. (Serpil Suvay, 2022)
- Chapter 11. Mitochondrial replacement therapy
- Barriers to mitochondrial replacement therapy
- Previous advancements
- Related complications and risks of MRT
- Examples of regulatory framework for MRT
- Different perspectives on MRT
- Conclusion
- Chapter 12. Application route of mitochondrial transplantation
- Introduction
- Application routes of mitochondrial transplantation
- Direct injection route for MT
- Systemic administration route for MT
- Intranasal administration route for MT
- Clinical reality of mitochondrial transplantation
- Dosing considerations
- Ethical challenges
- Conclusions
- Chapter 13. Limitations of mitochondrial transplantation
- Introduction
- Limitations
- Risk
- Limited efficacy
- Safety concern
- Worldwide regulations
- Ethical concern
- Conclusion
- Chapter 14. The ethical issues of mitochondrial transplantation and transfer
- Introduction
- Background
- Issues surrounding the concept of ‘parent’ and parentage
- Ethics and sense of self
- Donor anonymity and disclosure
- Germline genome modification, sex selection, and designer babies
- Conclusion
- Section V. Cardiovascular diseases and mitochondrial transplantation
- Chapter 15. Cardiovascular diseases and pathophysiology
- Concepts of cardiac output
- Heart failure and diabetes
- Conclusion
- Chapter 16. Cardiovascular diseases and mitochondrial transplantation: Basic and preclinical studies and transitions to clinical applications
- Introduction
- Pathology of the cardiovascular system associated with mitochondrial dysfunction
- Intercellular mitochondrial transfer in the cardiovascular system
- Conclusions
- Section VI. Neurological diseases and mitochondrial transplantation
- Chapter 17. Neurological diseases and pathophysiology
- Introduction
- Alzheimer's disease
- Parkinson's disease
- Amyotrophic lateral sclerosis
- Ischemic stroke
- Conclusion
- Chapter 18. Preclinical studies
- Introduction
- Conclusions
- Section VII. Other diseases and mitochondrial transplantation
- Chapter 19. Hereditary mitochondrial diseases and mitochondrial transplantation
- Hereditary mitochondrial diseases
- Mitochondrial transplantation in hereditary mitochondrial diseases
- Chapter 20. Mitochondrial transplantation in kidney injury
- Introduction
- An overview of acute kidney injury
- Mitochondria function and its importance in the kidney
- Mitochondria as a therapeutic target in kidney transplantation
- Mitochondrial transplantation: A novel technique in evolution
- Mitochondrial transplantation in the kidney
- Conclusion
- Acknowledgments
- Chapter 21. Cancer and mitochondrial transplantation, and novel insights into its application in the cancer-like phenotype of pulmonary arterial hypertension
- Mitochondrial transplantation
- Cancer
- Mitochondria and cancer
- Cancer-like phenotype of pulmonary artery hypertension
- Mitochondria transplantation in PAH: What is missing
- Conclusion
- Chapter 22. Diabetes and mitochondrial transplantation
- Introduction
- Mitochondrial transplantation in the diabetic heart
- Mitochondrial transplantation in the diabetic brain
- Mitochondrial transplantation in the diabetic kidney
- Mitochondrial transplantation in the diabetic liver
- Mitochondrial transplantation in pancreatic beta-islet cells
- Remaining questions and future applications for mitochondrial transplantation in the treatment of diabetes
- Chapter 23. Aging and mitochondrial transplantation
- Mitochondria and aging
- Conclusion
- Chapter 24. Mitochondrial transfer in the immune compartment
- Introduction
- Broad overview of the immune system
- Metabolic reprogramming during immune cell activation
- Mitochondrial dynamics and immune function
- Methods of intercellular mito-transfer
- Mito-transfer in innate immune cells
- Mito-transfer in adaptive immune cells
- Discussion
- Conclusion
- Chapter 25. The past, present, and close future of mitochondria as a therapeutic agent
- Introduction
- How mitochondria are exchanged from one cell to another
- History and present of AMT/T
- A transition from the present to the close future of AMT/T
- Concluding remarks
- Index
- Edition: 1
- Published: August 3, 2024
- No. of pages (Paperback): 560
- No. of pages (eBook): 430
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780443188589
- eBook ISBN: 9780443188596
GK
Gokhan Burcin Kubat
Gokhan Burcin Kubat currently serves as a Unit Manager in the Department of Mitochondria and Cellular Research, Gulhane Health Sciences Institute, University of Health Sciences, Turkey. He also attended the "Molecular Diagnostic Practicum" training at Virginia Commonwealth University, USA, and worked in the field of molecular pathology. He has contributed to research in mitochondrial transplantation and mitochondrial biogenesis with 10 peer reviewed publications, including in the Journal Molecular Medicine, Mitochondrion, Life Sciences, Biochimie, Journal of Biochemical and Molecular Toxicology, Toxicology and Applied Pharmacology, Cardiovascular Drugs and Therapy and Turk J Sports Med.
Affiliations and expertise
Department of Mitochondria and Cellular Research, Gulhane Health Sciences Institute, University of Health Sciences, Ankara, TurkeyOU
Oner Ulger
Oner Ulger currently serves in the Department of Mitochondria and Cellular Research, Gulhane Health Sciences Institute, University of Health Sciences in Ankara, Turkey. He got his doctorate from the Department of Physiology, Health Sciences Institute, University of Health Sciences in Turkey. He studies mitochondrial transplantation with high-impact research and review articles including in published in the Life Sciences, Biochimie, Journal of Biochemical and Molecular Toxicology, Toxicology and Applied Pharmacology, Mitochondrion, and Journal of Molecular Medicine.
Affiliations and expertise
Department of Mitochondria and Cellular Research, Gulhane Health Sciences Institute, University of Health Sciences, Ankara, TurkeySG
Serdar Gunaydin
Dr Gunaydin currently serves as a faculty and clinical professor in the Department of Cardiovascular Surgery in Ankara City Hospital Campus, University of Health Sciences, Turkey. He studied histology and embryology and received PhD in Biomaterial Sciences in Tokyo Heart Institute, Japan. He is the Vice President for International Society of Minimally Invasive Extracorporeal Circulation (MiECTIS) and General Secretary of Turkish Enhanced recovery After Surgery (ERAS) Society. He also works as the scientific board member in Giganode Foundation and Heart Team Education Association. He directs international scientific projects on clinical evaluation and biomaterial confirmation of novel cardiopulmonary bypass-related technologies based on the prevention of inflammatory response, patient blood management and ERAS.
Affiliations and expertise
Professor of Cardiovascular Surgery, University of Health Sciences, Ankara City Hospital Campus, TurkeyRead Mitochondrial Transplantation and Transfer on ScienceDirect