Thiamine and Thiamine-Pyrophosphate (TPP) Riboswitches
- 1st Edition, Volume 735 - October 1, 2026
- Latest edition
- Editors: Franck Charmantray, Bastien Doumèche
- Language: English
This volume of "Methods in Enzymology" series is devoted to the cofactor thiamine, also known as vitamin B1, and its phosphorylated derivatives. The biosynthesis of thiamine… Read more
Description
Description
This volume of "Methods in Enzymology" series is devoted to the cofactor thiamine, also known as vitamin B1, and its phosphorylated derivatives. The biosynthesis of thiamine is discussed in terms of key enzymes and their regulation, the evolution of metabolic pathways, and its importance in plant and yeast metabolism. The importance of quantifying and distinguishing between phosphorylated and unphosphorylated thiamine in animal and human tissues using chromatographic, enzymatic, and nanopore methods is also discussed. In addition to these methods, thiamine riboswitches are a major focus of this book, covering everything from their identification in plants and the elucidation of their structure to their application in detection.
This book presents state-of-the-art methods and detailed protocols intended for all researchers interested in thiamine metabolism or its detection.
This book presents state-of-the-art methods and detailed protocols intended for all researchers interested in thiamine metabolism or its detection.
Key features
Key features
- Overview of all methods to detect thiamine, with protocols.
- Methods to study and understand thiamine metabolism.
- All facets of thiamine riboswitches.
Readership
Readership
Biologist, biochemist, analytical chemist, physiologists and any researcher or students in life sciences.
Table of contents
Table of contents
Section 1: Thiamine metabolism and assays
1. Continuous directed evolution of thiazole synthase
Kristen Van Gelder, Edmar Ramos de Oliveira-Filho, Ulschan Bathe, Anuran Gayen and J Donato Garcia-Garcia
2. Evolutionary history of thiamine biosynthetic pathways in the domains of life and recent distribution and diversification: a bioinformatic approach
Marie-Anne Van Sluys, Henrique Moura-Dias and Naiara Toledo
3. Generation and characterization of thiamin-biofortified plants
Aymeric Goyer, David Handy and Keonny Cosek
4. Experimental approaches to study thiamine triphosphate and vitamin B1 derivative metabolism in plants
Kai Wang
5. ThiN as a versatile domain of transcriptional repressors and catalytic enzymes of thiamine biosynthesis
Julie A. Maupin-Furlow, Nicholas Cox and Katherine Weber
6. Thiamine deficiency on the oleaginous yeast Yarrowia lipolytica
Cong T. Trinh
7. Assessment of thiamine status in humans
Kyly Whitfield, Elizabeth Frank, Kerry S. Jones and Lucien Bettendorff
8. Assay of thiamine pyrophosphokinase activity
Lucien Bettendorff and Pierre Wins
9. Thiamine quantification in fish tissue samples by assaying transketolase activity
Katie Edwards
10. Engineered nanopore biosensor for thiamine quantification
Florian Leonardus Rudolfus Lucas, Carsten Wloka and Giovanni Maglia
Section 2: Riboswitches
11. Identification and characterisation of thiamine pyrophosphate (TPP) riboswitch in Elaeis guineensis
Zetty Norhana Binti Balia Yusof
12. Scaffold engineering enables high-resolution cryo-EM analysis of thiamine pyrophosphate riboswitch architecture and dynamics
Navtej Toor, Daniel Haack, Madison Maille and Boris Rudolfs
13. Design and applications of chimeric antisense oligonucleotides that bind thiamine pyrophosphate mRNAs as antibacterial agents
Robert Penchovsky
14. Functional analysis of thiamine pyrophosphate-responsive riboswitches using a specialized dual-luciferase reporter gene assay
Matthias Mack and Anna Hübenthal
15. Mapping small ligand-RNA interactions using transcriptome-wide sequencing
Willem A. Velema and Daphne van den Homberg
16. Monitoring structural dynamics of nascent bacterial riboswitches responding to TPP within transcriptional complexes
Daniel A. Lafontaine, Mirette Hashem and Carlos Penedo
1. Continuous directed evolution of thiazole synthase
Kristen Van Gelder, Edmar Ramos de Oliveira-Filho, Ulschan Bathe, Anuran Gayen and J Donato Garcia-Garcia
2. Evolutionary history of thiamine biosynthetic pathways in the domains of life and recent distribution and diversification: a bioinformatic approach
Marie-Anne Van Sluys, Henrique Moura-Dias and Naiara Toledo
3. Generation and characterization of thiamin-biofortified plants
Aymeric Goyer, David Handy and Keonny Cosek
4. Experimental approaches to study thiamine triphosphate and vitamin B1 derivative metabolism in plants
Kai Wang
5. ThiN as a versatile domain of transcriptional repressors and catalytic enzymes of thiamine biosynthesis
Julie A. Maupin-Furlow, Nicholas Cox and Katherine Weber
6. Thiamine deficiency on the oleaginous yeast Yarrowia lipolytica
Cong T. Trinh
7. Assessment of thiamine status in humans
Kyly Whitfield, Elizabeth Frank, Kerry S. Jones and Lucien Bettendorff
8. Assay of thiamine pyrophosphokinase activity
Lucien Bettendorff and Pierre Wins
9. Thiamine quantification in fish tissue samples by assaying transketolase activity
Katie Edwards
10. Engineered nanopore biosensor for thiamine quantification
Florian Leonardus Rudolfus Lucas, Carsten Wloka and Giovanni Maglia
Section 2: Riboswitches
11. Identification and characterisation of thiamine pyrophosphate (TPP) riboswitch in Elaeis guineensis
Zetty Norhana Binti Balia Yusof
12. Scaffold engineering enables high-resolution cryo-EM analysis of thiamine pyrophosphate riboswitch architecture and dynamics
Navtej Toor, Daniel Haack, Madison Maille and Boris Rudolfs
13. Design and applications of chimeric antisense oligonucleotides that bind thiamine pyrophosphate mRNAs as antibacterial agents
Robert Penchovsky
14. Functional analysis of thiamine pyrophosphate-responsive riboswitches using a specialized dual-luciferase reporter gene assay
Matthias Mack and Anna Hübenthal
15. Mapping small ligand-RNA interactions using transcriptome-wide sequencing
Willem A. Velema and Daphne van den Homberg
16. Monitoring structural dynamics of nascent bacterial riboswitches responding to TPP within transcriptional complexes
Daniel A. Lafontaine, Mirette Hashem and Carlos Penedo
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 735
- Published: October 1, 2026
- Language: English
About the editors
About the editors
FC
Franck Charmantray
Franck CHARMANTRAY is a researcher in Biocatalysis at the CNRS. He carries out his research work at the Institute of Chemistry in Clermont-Ferrand (ICCF, UMR6296, Clermont Auvergne University, France).
In 2001, he obtained a PhD in medicinal chemistry focusing on « A new family of DNA intercalating-alkylating agents » under the supervision of Dr. M. Demuynck, Grenoble 1 University, France. He then changed his research theme to focus on Studies on Homocitrate Synthase and other Lysine Pathway Enzymes during a postdoctoral internship funded by a BBRSC Fellowship, and carried out under the supervision of Prof. D. Young, at the University of Sussex, Brighton, UK. He returned to France to carry out a 2-year industrial postdoctoral internship in Biocatalysis financed by Laboratoires Fournier and which focused on the chemoenzymatic synthesis of antithrombotics. He was then recruited at the ICCF as a CNRS researcher in 2004. His research themes concern Biocatalysis, and in particular the study and optimization of transketolase by in vitro evolution for the synthesis of rare sugars and analogues in particular. He works in close collaboration with Dr. Bastien Doumèche with whom he recently developed an electrochemical screening for microbial transketolase inhibitors identification.
Affiliations and expertise
Researcher in Biocatalysis, CNRS, FranceBD
Bastien Doumèche
Claude Bernard Lyon 1 and ICBMS (Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR 5246 CNRS, France).After biochemistry and biology studies in Rouen (France), he obtained a master degree in Amiens on a biocatalysis topic, followed by a PhD at the RWTH-Aachen university (Germany) working on immobilized enzymes in two-phase systems under Prof. W. Hartemeier supervision. He joined the group of Dr. Rolland Furstoss and Dr. Alain Archelas at the CNRS in Marseille (France) for a post-doc on multi-gram scale biocatalysis using epoxide hydrolases. After a two-years temporary associate professor position at the university of Cergy-Pontoise (France) with Prof. Véronique Larreta-Garde on sol-gel transitions of protein-polysaccharides mixtures catalysed by enzymes, he became a lecturer at the University Lyon 1in 2006 where he developed original research in heterogeneous biocatalysis (ionic liquids) and more recently electrochemical screening assays using screen-printed electrodes for oxidoreductases (dehydrogenases, oxidases, laccases) and transketolases in collaboration with Dr. Franck Charmantray.
Affiliations and expertise
Claude Bernard Lyon 1 University, France