Skip to main content
Elsevier
View Cart

Books in Methodology techniques and instruments in biochemistry

  • Basic Life Science Methods

    A Laboratory Manual for Students and Researchers
    • 1st Edition
    • Javeed Ahmad Tantray + 3 more
    • English
    Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

  • Molecular Characterization of Autophagic Responses Part B

    • 1st Edition
    • Volume 588
    • English
    Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.

  • Introduction to Protein Mass Spectrometry

    • 1st Edition
    • Pradip K. Ghosh
    • English
    Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities.

  • Applications of NMR Spectroscopy: Volume 3

    • 1st Edition
    • Atta-ur Rahman + 1 more
    • English
    Applications of NMR Spectroscopy, Volume 3 presents the latest developments in the field of NMR spectroscopy, including the analysis of the structure-property relationship of polyphenols, breast cancer diagnosis, drug discovery and formulation, protein confirmation analysis using Fluorine NMR, and enaminone studies. The well-illustrated chapters contain comprehensive references to the recent literature. The content is ideal for readers who are seeking reviews and updates, as it consolidates scientific articles of a diverse nature into a single volume. The book is organized into sections based on disciplines such as food science and medical diagnostics, with each chapter written by eminent experts in the field. The applications presented cover a wide range of the field, such as drug development, medical imaging and diagnostics, food science, mining, petrochemical, process control, materials science, and chemical engineering, making this resource a multi-disciplinary reference.

  • Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A

    • 1st Edition
    • Volume 563
    • English
    Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.

  • Alcohol and Its Biomarkers

    Clinical Aspects and Laboratory Determination
    • 1st Edition
    • Amitava Dasgupta
    • English
    Alcohol and Its Biomarkers: Clinical Aspects and Laboratory Determination is a concise guide to all currently known alcohol biomarkers, their clinical application, and the laboratory methods used to detect them. Pathologists can use this resource to understand the limitations and cost factors associated with each method for determining certain alcohol biomarkers. In addition, interferences in these determinations are discussed, so that clinicians can understand the causes of falsely elevated biomarkers and pathologists and laboratory scientists can potentially eliminate them. The book focuses on the analytical methods used to detect alcohol in blood and urine, the limitations of alcohol determination using enzymatic methods, and the differences between clinical and forensic alcohol measurement. Chapters also cover cutting-edge alcohol biomarkers for potential use.

  • Histochemistry

    An Explanatory Outline of Histochemistry and Biophysical Staining
    • 1st Edition
    • Richard W. Horobin
    • English
    Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.

  • An Introduction to Medical Laboratory Technology

    • 1st Edition
    • F. J. Baker + 2 more
    • English
    An Introduction to Medical Laboratory Technology, Second Edition provides information pertinent to medical laboratory technology. This book discusses the importance of laboratory technology in hospital practice. Organized into seven sections encompassing 33 chapters, this edition begins with an overview of the role of the medical technologist in the diagnosis of disease by the use of certain accepted laboratory methods. This text then explains the general types of glassware that is widely used in medical laboratories. Other chapters consider the main methods of estimating the sugar content of body fluids, methods in feces and gastric analysis, and microscopical and chemical examination of urine. This book discusses as well the microscopic examination of bacteria, which necessitates making smears and hanging-drop preparations on microscope slides. The final chapter deals with some aspects of elementary physiology. This book is a valuable resource for students and junior technicians, as well as for qualified technologists and medical students.

  • Illustrated Guide for Theatre Nurses

    • 1st Edition
    • A. Marjorie Matthias + 2 more
    • English
    Illustrated Guide for Theatre Nurses provides practical instruction on how to perform correctly the many duties of a nurse. It presents the various procedures that are described in a series of photographs. This text begins with a series of photographs showing how the nurse should carry out her duties in the operating room. It then demonstrates the fundamental principles that underlie all modern operating techniques. This book is a valuable resource for nurses, technicians, student nurses, medical students, and clinical instructors.

  • Laboratory Methods in Enzymology: Protein Part C

    • 1st Edition
    • Volume 541
    • English
    In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.