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Elsevier

Books in Methodology techniques and instruments in biochemistry

  • Hyperspectral Imaging

    • 2nd Edition
    • Jose Manuel Amigo + 1 more
    • English
    Hyperspectral Imaging, Second Edition builds on the foundational insights of the first edition, covering core analytical frameworks and introducing new algorithms, hardware technologies, and cutting-edge applications. By incorporating the latest research findings and case studies, sections provide readers with enhanced tools and techniques for effective hyperspectral and multispectral image analysis across diverse scientific, industrial, and field domains. The book also addresses evolving challenges and fresh opportunities in the field, ensuring that users are equipped with the most current knowledge and practices. It underscores the continued relevance and transformative potential of hyperspectral and multispectral imaging in contemporary research and industry.Graduate students, academics, early researchers, and industry scientists across various disciplines working with hyperspectral and multispectral images, including analytical chemistry, remote sensing, vegetation and crops, food and feed production, forensic sciences, biochemistry, medical imaging, pharmaceutical production, art studies, cultural heritage, and more will find this update extremely useful.

  • Basic Life Science Methods

    A Laboratory Manual for Students and Researchers
    • 1st Edition
    • Javeed Ahmad Tantray + 3 more
    • English
    Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

  • Molecular Characterization of Autophagic Responses Part B

    • 1st Edition
    • Volume 588
    • English
    Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.

  • Applications of NMR Spectroscopy: Volume 3

    • 1st Edition
    • Atta-ur Rahman + 1 more
    • English
    Applications of NMR Spectroscopy, Volume 3 presents the latest developments in the field of NMR spectroscopy, including the analysis of the structure-property relationship of polyphenols, breast cancer diagnosis, drug discovery and formulation, protein confirmation analysis using Fluorine NMR, and enaminone studies. The well-illustrated chapters contain comprehensive references to the recent literature. The content is ideal for readers who are seeking reviews and updates, as it consolidates scientific articles of a diverse nature into a single volume. The book is organized into sections based on disciplines such as food science and medical diagnostics, with each chapter written by eminent experts in the field. The applications presented cover a wide range of the field, such as drug development, medical imaging and diagnostics, food science, mining, petrochemical, process control, materials science, and chemical engineering, making this resource a multi-disciplinary reference.

  • Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part B

    • 1st Edition
    • Volume 564
    • English
    Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.

  • Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A

    • 1st Edition
    • Volume 563
    • English
    Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.

  • Metabolic Analysis Using Stable Isotopes

    • 1st Edition
    • Volume 561
    • English
    Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes.

  • Biochemistry LabFax

    • 1st Edition
    • English
    LABFAX volumes are purpose-designed data reference books for practising scientists. Each book presents key information for a major subject in one place and so saves hours of searching. It does not simply collect together data which are already available in catalogues since these are often incomplete and can contain conflicting information. Rather, the authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs.Biochemistry LabFax is a detailed compendium of essential information - on radioisotopes, enzymes, proteins, nucleic acids, lipids, plus data on selected techniques such as chromatography, electrophoresis, etc. - needed on an almost daily basis by researchers in any area of biochemistry. In addition, the book acts as a gateway to other sources for information more specific than can be covered in a volume of this size.

  • Histochemistry

    An Explanatory Outline of Histochemistry and Biophysical Staining
    • 1st Edition
    • Richard W. Horobin
    • English
    Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.

  • Illustrated Guide for Theatre Nurses

    • 1st Edition
    • A. Marjorie Matthias + 2 more
    • English
    Illustrated Guide for Theatre Nurses provides practical instruction on how to perform correctly the many duties of a nurse. It presents the various procedures that are described in a series of photographs. This text begins with a series of photographs showing how the nurse should carry out her duties in the operating room. It then demonstrates the fundamental principles that underlie all modern operating techniques. This book is a valuable resource for nurses, technicians, student nurses, medical students, and clinical instructors.

  • An Introduction to Medical Laboratory Technology

    • 1st Edition
    • F. J. Baker + 2 more
    • English
    An Introduction to Medical Laboratory Technology, Second Edition provides information pertinent to medical laboratory technology. This book discusses the importance of laboratory technology in hospital practice. Organized into seven sections encompassing 33 chapters, this edition begins with an overview of the role of the medical technologist in the diagnosis of disease by the use of certain accepted laboratory methods. This text then explains the general types of glassware that is widely used in medical laboratories. Other chapters consider the main methods of estimating the sugar content of body fluids, methods in feces and gastric analysis, and microscopical and chemical examination of urine. This book discusses as well the microscopic examination of bacteria, which necessitates making smears and hanging-drop preparations on microscope slides. The final chapter deals with some aspects of elementary physiology. This book is a valuable resource for students and junior technicians, as well as for qualified technologists and medical students.

  • Laboratory Methods in Enzymology: Protein Part C

    • 1st Edition
    • Volume 541
    • English
    In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

  • Bio-nanoimaging

    Protein Misfolding and Aggregation
    • 1st Edition
    • Vladimir N. Uversky + 1 more
    • English
    Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering:- Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobuli... studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin oligomers- Polymorphisms of misfolding and aggregation processes, including multiple pathways of lysozyme aggregation, misfolded intermediate of a PDZ domain, and micelle formation by human islet amyloid polypeptide Protein misfolding and aggregation is a fast-growing frontier in molecular medicine and protein chemistry. Related disorders include cataracts, arthritis, cystic fibrosis, late-onset diabetes mellitus, and numerous neurodegenerative diseases like Alzheimer's and Parkinson's. Nanoimaging technology has proved crucial in understanding protein-misfolding pathologies and in potential drug design aimed at the inhibition or reversal of protein aggregation. Using these technologies, researchers can monitor the aggregation process, visualize protein aggregates and analyze their properties.

  • Recombinant DNA Methodology II

    • 1st Edition
    • Ray Wu
    • English
    The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

  • Metabolomics Coming of Age with its Technological Diversity

    • 1st Edition
    • Volume 67
    • English
    Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 67th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on metabolomics coming of age with its technological diversity.

  • Nucleosomes, Histones and Chromatin Part A

    • 1st Edition
    • Volume 512
    • English
    This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

  • Imaging and Spectroscopic Analysis of Living Cells

    Optical and Spectroscopic Techniques
    • 1st Edition
    • Volume 504
    • English
    This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

  • Methods in Systems Biology

    • 1st Edition
    • Volume 500
    • English
    Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.

  • Cryo-EM, Part C

    Analyses, Interpretation, and Case Studies
    • 1st Edition
    • Volume 483
    • English
    This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

  • Cryo-EM Part B: 3-D Reconstruction

    • 1st Edition
    • Volume 482
    • English
    This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

  • Micro Systems and Devices for (Bio)chemical Processes

    • 1st Edition
    • Volume 38
    • English
    This volume of Advances in Chemical Engineering presents the latest developments in microsystems and devices for biochemical processes.

  • Guide to Protein Purification

    • 2nd Edition
    • Volume 436
    • Richard R Burgess + 1 more
    • English
    Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today.

  • Guide to Protein Purification

    • 2nd Edition
    • Volume 463
    • Richard R Burgess + 1 more
    • English
    Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today.

  • Fluorescence Spectroscopy

    • 1st Edition
    • Volume 450
    • English
    Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics.

  • Imaging in Biological Research, Part B

    • 1st Edition
    • Volume 386
    • English
    This volume addresses current methods in biological imaging, including extensive sections on MRI, CAT, NMR, PET and other imaging techniques.

  • Inositol Phospholipid Metabolism and Phosphatidyl Inositol Kinases

    • 1st Edition
    • Volume 30
    • A. Kuksis
    • English
    This book offers a wide ranging and review of cutting edge developments along with tried and tested methods for isolation, resolution and quantification of inositol phospholipids and inositol polyphosphates in both cells and tissues. It includes detailed and rigorous methodology for identification of molecular species of inositol phospholipids, including their phosphates and glycans, with numerous examples of specific applications. Wherever available, both chromatographic and mass spectrometricevidenc... is presented for specific phospholipid involvement in the biochemical transformations accompanying metabolic signalling events and, where possible, controversies have been resolved on basis of analytical ambiguity. The review of the lipidanalyses extends to products of phosphatidylinositol kinases, phosphatases and lipases. A general review chapter is included on metabolic signalling with special emphasis on the lipid products of 3-kinases. In view of the general lack of commercially available standards for inositol phospholipids and inositol phosphates, the book concludes with a chapter on the preparation and collection of chromatographic and mass spectrometric proof of their purity and identity.This book complements recent books and reviews on the mechanisms metabolic signalling, receptor binding and the polypeptide structure of the proteins involved in the various signalling pathways. It also complements those texts that deal exclusively with the chemical synthesis of the phosphatidylinositol... and their polyphosphates and glycans.

  • Cancer Metastasis: Experimental Approaches

    • 1st Edition
    • Volume 29
    • D. Rusciano + 1 more
    • D.R. Welch
    • English
    The book describes most of the methods that are currently used in metastasis research. Both in vivo and in vitro protocols are illustrated, so that the metastatic process can be either analysed as a whole, or single events addressed separately. Each method is described in the frame of the metastatic process, therefore its significance and its limitations in the context of metastasis are always taken into account. Whenever possible, several alternative procedures are reported per each experimental issue, so that the researcher can choose the one that better suits her/his needs and possibilities.During the past 30 years a big effort has been made to elucidate the molecular mechanisms of cancer metastatis, the leading cause of death for cancer patients. A considerable number of assays have been set up, that can be used to address specific questions concerning the single metastatic steps, or can be applied to develop and test drugs specifically interfering with selected events during the metastatic spread. This book contains an exhaustive description of most of the methods and their rationale, that are currently used in metastatic research, both to analyse metastasis in its entirety (in vivo models), or to dissect the single steps of the metastatic process (in vitro assays).

  • Ultrasensitive and Rapid Enzyme Immunoassay

    • 1st Edition
    • Volume 27
    • E. Ishikawa
    • English
    Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as well as ultrasensitive enzyme immunoassays. Ultrasensitive and Rapid Enzyme Immunoassay, with its clear presentation and up-to-date information, will be essential to scientists in pharmaceutical companies and in applied research.

  • Advances in Lipid Methodology

    • 1st Edition
    • Volume 4
    • W. W. Christie
    • English
    This is the fourth volume of an occasional series of review volumes dealing with aspects of lipid methodology. As with the first three volumes, topics have been selected that have been developing rapidly in recent years and have some importance to lipid analysis. The authors are all leading international experts.Topics covered include: analysis of plant lipoxygenase metabolites, preparative high-performance liquid chromatography of lipids, structural analysis of fatty acids, and analysis of stable isotopes in lipids, among others.

  • An Introduction to Radioimmunoassay and Related Techniques

    • 5th Edition
    • Volume 6
    • T. Chard
    • English
    Immunoassay procedures (isotopic and non-isotopic) have become one of the single most important techniques in present-day diagnostic medicine. This book is designed as an introductory test for the staff of clinical research laboratories who conduct or intend to conduct such techniques, and will be of great value to the clinicians who make use of such services. The volume takes a three-pronged approach in it's in-depth presentation: explanation of the basic principles and applications of radioimmunoassays and non-isotopic immunoassays; practical illustrations of the various steps involved in immunoassays; discussion of the problems and pitfalls in immunoassays and how to avoid them. This fifth revised edition is a worthy successor to it's predecessors in this famous "Laboratory Techniques" series.

  • Hemoglobins, Part B: Biochemical and Analytical Methods

    • 1st Edition
    • Volume 231
    • John N. Abelson + 1 more
    • English
    Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago.

  • Nitrogen Isotope Techniques

    • 1st Edition
    • English
    This book is the first laboratory manual to bring together basic procedures for measurement of stable and radioactive isotopes of nitrogen, with specific applications to plant, soil, and aquatic biology. This bench-top reference gives practical coverage of mass and emission spectrometry, nitrogen fixation, nitrification, and identification, organic nitrogen, and the radioactive isotope 13N. Methods are described so that researchers can adapt them, without the aid of outside references, to virtually any task they may encounter in investigations of nitrogen transformation processes.

  • Numerical Computer Methods

    • 1st Edition
    • Volume 210
    • John N. Abelson + 1 more
    • English
    The aim of this volume is to brief researchers of the importance of data analysis in enzymology, of the modern methods that have developed concomitantly with computer hardware, and of the need to validate their computer programs with real and synthetic data to ascertain that the results produced are what they expected.

  • Techniques in Protein Chemistry

    • 1st Edition
    • Tony E. Hugli
    • English
    Techniques in Protein Chemistry is based on the papers presented on the second annual meeting of the Protein Society in August 1988. The compendium contains the significant technical advances in protein chemistry. The book highlights topics that are of general interest and practical value to protein chemists. Emphasis is given to methods and applications in protein sequencing; applications of mass spectrometry and nuclear magnetic resonance technology; limitations of amino acid microanalysis; and high-performance liquid chromatography. The book will be a good reference for chemists and researchers in the field of protein chemistry.

  • Modern Physical Methods in Biochemistry, Part B

    • 1st Edition
    • Volume 11B
    • L.L.M. van Deenen + 1 more
    • English
    This volume completes the account of physical methods used in biochemistry that was covered in Volume 11A, which was published in 1985. It deals with fast reaction methods, high performance liquid chromatography of peptides and proteins, fluorescence spectroscopy, neutron and X-ray scattering techniques, and also Raman and resonance spectroscopy which have in recent years been used to an increasing extent in biochemistry. This addition to the invaluable series New Comprehensive Biochemistry will be of interest to all those working in the fields of biochemistry and biophysics.

  • Applications of HPLC in Biochemistry

    • 1st Edition
    • Volume 17
    • A. Fallon + 2 more
    • English
    This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.

  • Enzyme Purification and Related Techniques, Part C

    • 1st Edition
    • Volume 104
    • Nathan P. Kaplan + 1 more
    • English
    The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

  • Preparation and Assay of Enzymes

    • 1st Edition
    • Volume 2
    • Nathan P. Kaplan + 1 more
    • English
    The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

  • Preparation and Assay of Enzymes

    • 1st Edition
    • Volume 1
    • Nathan P. Kaplan + 1 more
    • English
    The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.