Books in Methodology techniques and instruments in biochemistry

Basic Life Science Methods

1st Edition
September 11, 2022
Javeed Ahmad Tantray + 3 more
English
Paperback
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eBook
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Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

Molecular Characterization of Autophagic Responses Part B

1st Edition
Volume 588
February 22, 2017
Lorenzo Galluzzi + 2 more
English
Hardback
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eBook
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Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.

Introduction to Protein Mass Spectrometry

1st Edition
December 7, 2015
Pradip K. Ghosh
English
Hardback
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eBook
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Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities.

Applications of NMR Spectroscopy: Volume 3

1st Edition
November 23, 2015
Atta-ur Rahman + 1 more
English
Paperback
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eBook
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Applications of NMR Spectroscopy, Volume 3 presents the latest developments in the field of NMR spectroscopy, including the analysis of the structure-property relationship of polyphenols, breast cancer diagnosis, drug discovery and formulation, protein confirmation analysis using Fluorine NMR, and enaminone studies. The well-illustrated chapters contain comprehensive references to the recent literature. The content is ideal for readers who are seeking reviews and updates, as it consolidates scientific articles of a diverse nature into a single volume. The book is organized into sections based on disciplines such as food science and medical diagnostics, with each chapter written by eminent experts in the field. The applications presented cover a wide range of the field, such as drug development, medical imaging and diagnostics, food science, mining, petrochemical, process control, materials science, and chemical engineering, making this resource a multi-disciplinary reference.

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part B

1st Edition
Volume 564
October 7, 2015
Peter Z Qin + 1 more
English
Hardback
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eBook
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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A

1st Edition
Volume 563
October 5, 2015
Peter Z Qin + 1 more
English
Hardback
9 7 8 0 1 2 8 0 2 8 3 4 6
eBook
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Metabolic Analysis Using Stable Isotopes

1st Edition
Volume 561
September 8, 2015
Christian Metallo
English
Hardback
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eBook
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Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes.

Laboratory Methods in Enzymology: Protein Part D

1st Edition
Volume 559
June 9, 2015
Jon Lorsch
English
Hardback
9 7 8 0 1 2 8 0 0 2 7 9 7
eBook
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The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts.

Alcohol and Its Biomarkers

1st Edition
February 18, 2015
Amitava Dasgupta
English
Hardback
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eBook
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Alcohol and Its Biomarkers: Clinical Aspects and Laboratory Determination is a concise guide to all currently known alcohol biomarkers, their clinical application, and the laboratory methods used to detect them. Pathologists can use this resource to understand the limitations and cost factors associated with each method for determining certain alcohol biomarkers. In addition, interferences in these determinations are discussed, so that clinicians can understand the causes of falsely elevated biomarkers and pathologists and laboratory scientists can potentially eliminate them. The book focuses on the analytical methods used to detect alcohol in blood and urine, the limitations of alcohol determination using enzymatic methods, and the differences between clinical and forensic alcohol measurement. Chapters also cover cutting-edge alcohol biomarkers for potential use.

Laboratory Methods in Enzymology: Protein Part C

1st Edition
Volume 541
March 22, 2014
Jon Lorsch
English
Hardback
9 7 8 0 1 2 4 2 0 1 1 9 4
eBook
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In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

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