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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A
1st Edition - October 5, 2015
Editors: Peter Z Qin, Kurt Warncke
Hardback ISBN:9780128028346
9 7 8 - 0 - 1 2 - 8 0 2 8 3 4 - 6
eBook ISBN:9780128028469
9 7 8 - 0 - 1 2 - 8 0 2 8 4 6 - 9
Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the… Read more
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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.
Timely contribution that describes a rapidly changing field
Leading researchers in the field
Broad coverage: Instrumentation, basic theory, data analysis, and applications
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
Preface
Section I: EPR Methodology and Instrumentation
Chapter One: Rapid-Scan EPR of Nitroxide Spin Labels and Semiquinones
Abstract
1 Introduction
2 Rapid-Scan Method
3 Why Rapid Scan Improves S/N
4 Other Advantages of Rapid Scan
5 Hardware Requirements
6 Parameter Selection
7 Examples
8 Wider Scans
9 Future Applications
Acknowledgments
Chapter Two: Fourier Deconvolution Methods for Resolution Enhancement in Continuous-Wave EPR Spectroscopy
Abstract
1 Introduction
2 Historical Perspective
3 Overlapping Signals
4 Fourier Filtering
5 Overview of Method
6 Practical Examples
7 Limitations of the Method
8 Summary
Acknowledgment
Supplementary materials
Chapter Three: Multifrequency Pulsed EPR and the Characterization of Molecular Dynamics
Abstract
1 Introduction
2 Contributions to Electron Spin-Lattice Relaxation and Dependence of Relaxation on Motions in Fluid Solution
3 Measurement of Spin-Lattice Relaxation, T1
4 Examples of Motions Elucidated by Measuring T1 as a Function of Frequency
5 Conclusion
Acknowledgments
Chapter Four: Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low-Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution Systems
Abstract
1 Introduction
2 Low-Temperature Fluid Cryosolvent System
3 Low-Temperature Frozen Solution System
4 Analysis and Interpretations: General Cases and Guidelines
5 Conclusions
Acknowledgments
Chapter Five: 230/115 GHz Electron Paramagnetic Resonance/Double Electron–Electron Resonance Spectroscopy
Abstract
1 Introduction
2 HF EPR/DEER Spectrometer
3 Examples of HF EPR: Paramagnetic Impurities in Diamonds Studied by 230 GHz EPR/DEER Spectroscopy
4 Summary
Acknowledgments
Appendix
Section II: EPR Spectral Simulation and Software
Chapter Six: CW-EPR Spectral Simulations: Solid State