Basic Life Science Methods
A Laboratory Manual for Students and Researchers
- 1st Edition - September 11, 2022
- Imprint: Academic Press
- Authors: Javeed Ahmad Tantray, Nighat Un Nissa, Rasy Fayaz Choh Wani, Sheikh Mansoor
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 1 9 1 7 4 - 9
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 9 1 7 5 - 6
Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cov… Read more
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Request a sales quoteBasic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.
- Provides a practical resource on designing, executing and analyzing experiments and analytical procedures
- Includes detailed and standardized coverage of basic research methods in the area
- Presents step-by-step instructions on how to execute a large selection of life sciences experiments
Graduate students and early career researcher in life sciences. Life sciences laboratory staff members looking for resources to update their practices
- Cover image
- Title page
- Table of Contents
- Copyright
- List of figures
- List of tables
- About the authors
- Foreword
- Foreword
- Introduction
- Chapter 1. Calculations of molarity and normality
- Molarity (M)
- Normality (N)
- Chapter 2. Glassware washing and sterilization techniques
- Principle
- Washing of glasswares is done by two main methods
- General procedure for cleaning of glasswares by detergent
- General procedure for cleaning of glasswares by chromic acid
- Preparation of 5% chromic acid
- Cleaning by chromic acid
- Types of sterilization
- Observation and results
- Chapter 3. pH meter: Its use and calibration
- Structure
- Calibration
- Observation and result
- Chapter 4. Principle and working of autoclave
- Conditions for autoclave sterilization
- Chapter 5. Principle and working of centrifuge
- Rate of sedimentation
- Sedimentation time
- Parts of centrifuge
- Relative centrifugal force
- Types of centrifuges
- Type of centrifugations
- Chapter 6. Principle and working of laminar
- Parts of a laminar air flow chamber (Fig. 6.1)
- Advantages of a laminar air flow chamber
- Disadvantages of a laminar air flow chamber
- Applications of a laminar air flow chamber
- When employing a laminar air flow chamber, there are a few things to keep in mind
- Chapter 7. Estimation of total cholesterol (Flegg, 1972)
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 8. Estimation of creatinine
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Alternative methods/procedures
- Chapter 9. Estimation of blood urea
- Materials and equipment
- Step-by-step method details
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Alternative methods/procedures
- Chapter 10. Estimation of blood glucose by GOD—POD method
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Normal range blood
- Advantages
- Limitations
- Safety considerations and standards
- Alternative methods/procedures
- Chapter 11. Determination of albumin and globulin in plasma
- Materials and equipment (materials and reagents)
- Chapter 12. Quantitative determination of homocysteine
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 13. Estimation of serum bilirubin
- Reagents required
- Materials required
- Reagent preparation
- Assay procedure
- Assay procedure
- Chapter 14. Estimation of diagnostic enzyme ALT
- Reagents
- Materials required
- Regent preparation
- Specimen
- Assay procedure
- Assay procedure 2
- Calculations
- Quality control
- Performance characteristics
- Temperature conversion factors
- Chapter 15. Estimation of diagnostic enzyme AST
- Reagents
- Materials required
- Regent preparation
- Specimen
- Assay procedure
- Sample/control
- Assay procedure 2
- Sample/control
- Calculations
- Quality control
- Performance characteristics
- Chapter 16. Protein estimation by Lowry's method
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Alternative methods/procedures
- Chapter 17. Estimation of reducing sugar by using dinitro salicylic acid method
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Alternative methods/procedures
- Chapter 18. To perform quantitative determination of total protein in serum/plasma
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Alternative methods/procedures
- Chapter 19. Enzyme activity using starch assay
- Requirements
- Preparation
- Procedure
- Observation and result
- Determination of enzyme activity
- Chapter 20. To estimate protein by Bradford assay
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 21. To identify a protein sample using SDS-PAGE
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 22. Genomic DNA isolation from whole blood
- Principle
- Materials and equipment (materials and reagents) (Tables 22.1–22.3)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 23. PCR for gene amplification
- Materials and equipment
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 24. Agarose gel electrophoresis
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 25. RNA isolation from human tissue
- Some important precautions
- Requirements
- Protocol
- Optimized protocol for the extraction of RNA isolation from blood
- Chapter 26. Real-time polymerase chain reaction
- Types of real-time quantification
- Materials and equipment
- Step-by-step method details
- Relative gene expression studies
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 27. Preparation of competent cells by CaCl2 treatment
- Principle
- Materials required
- Equipment
- Principle
- Preparation of media or solution
- Procedure
- Methods
- Heat shock transformation of Escherichia coli
- Methods
- Chapter 28. RNA isolation from plant tissues
- DNase treatment
- cDNA synthesis
- Chapter 29. DNA isolation from the bacterial culture broth
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 30. DNA isolation from fungal mycelium
- Introduction
- Procedure or steps
- Precautions
- Chapter 31. Quantification of the DNA using spectrophotometer
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 32. Formaldehyde gel electrophoresis of the isolated RNA sample
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 33. Paper chromatography of different amino acid
- Materials required for setup of technique
- Experimental procedure
- Biosafety considerations and laboratory standards
- The experimental outcome
- Advantages
- Limitations
- Chapter 34. Separation of plant pigments using column chromatography
- Basic principle behind separation of biomolecules
- Materials and equipment (materials and reagents)
- Chemical solutions used for column chromatography
- Step-by-step procedure
- Expected outcomes
- Advantages of column chromatography
- Main limitations
- Safety concerns and experimental standards
- Alternative methods/procedures
- Chapter 35. Separation of plant pigment by thin-layer chromatography
- Materials required
- Reagents
- Procedure
- Observation and results
- Chapter 36. Chromatin immunoprecipitation protocol
- Materials
- Procedure
- Precautions
- Chapter 37. Types of media
- Chapter 38. Preparation of nutrient agar media
- Principle
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 39. Serial dilution of the bacterial cells
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 40. Spread plate method of the bacterial cells
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 41. Streak plate method
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 42. Identified bacterial colony in culture broth
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 43. Pour plate method for bacterial colony counting
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 44. Gram staining of bacteria
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 45. Casein hydrolysis test
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Safety considerations and standards
- Chapter 46. Antibiotic resistance test by agar well diffusion method
- Materials and equipment (materials and reagents)
- Step-by-step method details (experimental procedure)
- Expected outcomes
- Advantages
- Limitations
- Index
- Edition: 1
- Published: September 11, 2022
- Imprint: Academic Press
- No. of pages: 230
- Language: English
- Paperback ISBN: 9780443191749
- eBook ISBN: 9780443191756
JT
Javeed Ahmad Tantray
Dr. Javeed Ahmad Tantray’s current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist awards both at national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome,with an award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).
Affiliations and expertise
Assistant Professor, Department of Zoology, Central University of Kashmir, IndiaNN
Nighat Un Nissa
Nighat Un Nissa has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir.
Affiliations and expertise
Assistant Professor, Department of Zoology, University of Kashmir, IndiaRW
Rasy Fayaz Choh Wani
Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”.
Affiliations and expertise
Research Scholar, Department of Zoology, University of Kashmir, IndiaSM
Sheikh Mansoor
Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Dr. Mansoor has been dedicated to contributing significantly to his field and has displayed a strong commitment to becoming a highly accomplished scientist. His expertise spans various areas, including biochemistry, molecular biology, and genetics. Dr. Mansoor's accomplishments are notable, including over more than 50 research and review papers published in esteemed journals such as Frontiers, Plos One, Scientific Reports, Journal of Fungi (JoF), Planta, Chemosphere, JEMA and Molecules etc. He has also authored 5 books and more than ten book chapters, and holds credit for filing two patent applications. He has showcased his knowledge through guest lectures on topics like sequence assembly, alignment, submission tools, and phylogenetic analysis. He has received practical training in advanced techniques in modern biology and related fields. Dr. Mansoor has been recognized with numerous national and international accolades, including prestigious awards such as Best Thesis, Young Scientist, and recognition for his exceptional oral and poster presentations. He holds membership in a significant big grant (BIRAC) awarded by the Department of Biotechnology (DBT) in India. In addition to his accomplishments, Dr. Mansoor plays an active role as a Review Editor for Frontiers in Ethnopharmacology, and he also contributes as a reviewer for esteemed journals like 3 Biotech, Cell Reports, Frontiers, Plos One, and JARMAP. His unwavering dedication, profound expertise, and active involvement underscore his unwavering commitment to advancing scientific knowledge in his field.
Dr. Sheikh Mansoor currently serves as a
Postdoctoral Researcher at the Department
of Plant Resources and Environment and
Subtropical and Tropical Organism Gene
Bank within Jeju National University in
South Korea. Prior to this role, he held
positions as a Postdoc Research Associate at
SKIMS Srinagar and as a Project Associate-I
at CSIR-Indian Institute of Integrative Medicine
Jammu (IIIM). Throughout his academic
journey, Dr. Mansoor has had the
opportunity to work in reputed laboratories,
including the Transcriptomics Labs at
SKUAST Kashmir and the Department of
Biotechnology and Pathology at Central
Institute of Temperate Horticulture (CITH),
Rangreth, Srinagar. His educational background
comprises a BSc degree from SP
College of Science (University of Kashmir),
an MSc degree from HNBGU, Uttarakhand,
and a PhD degree from Sher-e-Kashmir
University of Agricultural Sciences and
Technology of Jammu. Dr. Mansoor has been
dedicated to contributing significantly to his
field and has displayed a strong commitment
to becoming a highly accomplished
scientist. His expertise spans various areas,
including biochemistry, molecular biology,
and genetics. Dr. Mansoor’s accomplishments
are notable, including more than 50
research and review papers published in
esteemed journals such as Frontiers, PLOS
One, Scientific Reports, Journal of Fungi (JoF),
Planta, Chemosphere, JEMA, Molecules etc. He
has also authored 5 books and more than 10
book chapters and holds credit for filing two
patent applications. He has showcased his
knowledge through guest lectures on topics
like sequence assembly, alignment, submission
tools, and phylogenetic analysis. He has
received practical training in advanced
techniques in modern biology and related
fields. Dr. Mansoor has been recognized
with numerous national and international
accolades, including prestigious awards such
as Best Thesis, Young Scientist, and recognition
for his exceptional oral and poster
presentations. He holds membership in a significant big grant (BIRAC) awarded by
the Department of Biotechnology (DBT) in
India. In addition to his accomplishments,
Dr. Mansoor plays an active role as a Review
Editor for Frontiers in Ethnopharmacology, and
he also contributes as a reviewer for
esteemed journals like 3 Biotech, Cell Reports,
Frontiers, PLOS One, and JARMAP. His
unwavering dedication, profound expertise,
and active involvement underscore his unwavering commitment to advancing scientific
knowledge in his field.
Affiliations and expertise
Postdoctoral Researcher, Phenomics Laboratory, Department of Plant Resources and Environment, Jeju National University, South KoreaRead Basic Life Science Methods on ScienceDirect