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Basic Life Science Methods
A Laboratory Manual for Students and Researchers
1st Edition - September 11, 2022
Authors: Javeed Ahmad Tantray, Nighat Un Nissa, Rasy Fayaz Choh Wani, Sheikh Mansoor Shafi
Paperback ISBN:9780443191749
9 7 8 - 0 - 4 4 3 - 1 9 1 7 4 - 9
eBook ISBN:9780443191756
9 7 8 - 0 - 4 4 3 - 1 9 1 7 5 - 6
Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to… Read more
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Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.
Provides a practical resource on designing, executing and analyzing experiments and analytical procedures
Includes detailed and standardized coverage of basic research methods in the area
Presents step-by-step instructions on how to execute a large selection of life sciences experiments
Graduate students and early career researcher in life sciences. Life sciences laboratory staff members looking for resources to update their practices
Cover image
Title page
Table of Contents
Copyright
List of figures
List of tables
About the authors
Foreword
Foreword
Introduction
Chapter 1. Calculations of molarity and normality
Molarity (M)
Normality (N)
Chapter 2. Glassware washing and sterilization techniques
Principle
Washing of glasswares is done by two main methods
General procedure for cleaning of glasswares by detergent
General procedure for cleaning of glasswares by chromic acid
Preparation of 5% chromic acid
Cleaning by chromic acid
Types of sterilization
Observation and results
Chapter 3. pH meter: Its use and calibration
Structure
Calibration
Observation and result
Chapter 4. Principle and working of autoclave
Conditions for autoclave sterilization
Chapter 5. Principle and working of centrifuge
Rate of sedimentation
Sedimentation time
Parts of centrifuge
Relative centrifugal force
Types of centrifuges
Type of centrifugations
Chapter 6. Principle and working of laminar
Parts of a laminar air flow chamber (Fig. 6.1)
Advantages of a laminar air flow chamber
Disadvantages of a laminar air flow chamber
Applications of a laminar air flow chamber
When employing a laminar air flow chamber, there are a few things to keep in mind
Chapter 7. Estimation of total cholesterol (Flegg, 1972)
Dr. Javeed Ahmad Tantray’s current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist awards both at national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome,with an award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).
Affiliations and expertise
Assistant Professor, Department of Zoology, Central University of Kashmir, India
NN
Nighat Un Nissa
Nighat Un Nissa has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir.
Affiliations and expertise
Assistant Professor, Department of Zoology, University of Kashmir, India
RW
Rasy Fayaz Choh Wani
Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”.
Affiliations and expertise
Research Scholar, Department of Zoology, University of Kashmir, India
SS
Sheikh Mansoor Shafi
Dr. Sheikh Mansoor Shafi is a postdoctoral researcher, presently working at the SK- Institute of Medical Sciences Soura Srinagar, India. Previously he was working as project associate-I at the CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). He obtained his PhD from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Dr. Mansoor has made important contributions to his field and has an impressive lab experience in biochemistry, molecular biology, and genetics. He has published more than 30 research and review papers in reputed journals. He has published more than 10 book chapters, 3 books and he has filed one patent application.
Affiliations and expertise
The Department of Advanced Centre for Human Genetics, Sher-i-Kashmir Institute of Medical Sciences, Soura Srinagar Kashmir, India