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Books in Molecular biology cellular biology and genetics

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    • Lipids and Membranes: Detection by Imaging and Lipidomics

      • 1st Edition
      • Volume 726
      • English
      Lipids and Membranes: Detection by Imaging and Lipidomics, Volume 727 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the chapters in this new release include Measurement of fatty acid esters of hydroxy fatty acids, Superresolution organelle imaging with lipophilic HIDE probes, Ultra-high resolution analysis of membrane lipids from 15N metabolically labeled mice, Superresolution lipid imaging with expansion microscopy, High-resolution phosphoinositide analysis in healthy versus disease cells, and much more.Additional chapters focus on Alkyne lipids as tracers of lipid metabolism, Imaging oxidative stress in biomembranes, Imaging the dynamics of phosphatidylinositol 4-phosphate metabolism, Lipidomics for studying sphingolipids in cell death, Non-invasive imaging of phosphoinositides with genetically encoded lipid biosensors, Profiling lipotypes at the single cell level in heterogenous populations, MS imaging of phosphoinositides, and much more.

    • Lipids and Membranes: Dynamics and Interorganelle Lipid Transport

      • 1st Edition
      • Volume 727
      • English
      Lipids and Membranes: Dynamics and Interorganelle Lipid Transport, Volume 728 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the book's chapters include topics such as Assay of phospholipid scrambling, Tracking lipid transport with bifunctional lipids, Tracking interorganelle lipid transport using genetically encoded mass tags, Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains, Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation, Studying interactions of amphipathic helices with lipid droplets, and more.Additional chapters cover Metabolic labeling of to trace changes in sphingolipid metabolism, Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing, Tagging lipoproteins in mycobacteria, Probes for studying sphingolipid dynamics, Measuring Flipper-TR Lifetime with Multiple Analysis Strategies, Reconstitution approaches to understanding cellular lipid dynamics, Prediction and characterization of the bacterial lipid handling machinery, From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico, and much more.

    • Immuno-oncology and Immunotherapy Part G

      • 1st Edition
      • Volume 202
      • English
      Immuno-oncology and immunotherapy, Part G, Volume 202 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.

    • Cell Shape and Position during Development

      • 1st Edition
      • Volume 166
      • English
      Cell Shape and Position during Development, Volume 166 provides a comprehensive overview of the fundamental roles that cell morphology and spatial organization play in the development of multicellular organisms. This volume brings together leading experts in developmental biology, cell biology, and biophysics to explore how cells acquire specific shapes. Chapters in this new release include Cell shape changes during sperm-egg interactions, Cell shape and position during organ development, Regulation of shape and position during asymmetric cell division, Cell shape dynamics during mouse peri-implantation development and embryo dormancy, Shaping the intestine: the role of cell morphology and spatial dynamics in development, and more.Additional sections Intermediate filaments in cell shape and position, Regulation of adult stem cell behavior by cytoskeletal dynamics and cell shape, Shape changes in gastrulation, and more.

    • Design and Applications of Cell-Free Systems Part B

      • 1st Edition
      • Volume 219
      • English
      Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

    • Immuno-oncology and Immunotherapy Part F

      • 1st Edition
      • Volume 201
      • English
      Radiation Oncology and Radiotherapy Part B, Volume 174 of Methods in Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

    • Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions

      • 1st Edition
      • Volume 728
      • English
      Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions, Volume 729 provides a broad snapshot of state-of-the-art methods for studying biological lipids and biomembranes. Sections in this new release cover De novo chemoenzymatic construction of lipid membranes, Photoaffinity labeling for cholesterol metabolism, Expression and Characterization of Phospholipase Cb and Ce enzymes, Assembling Retromer-coated membrane tubules for in vitro structure-function studies, Quantitative analysis of S-acylation, Inhibition of palmitoyltransferase... Exploiting protein lipidation to create peptide-based biomaterials, and much more.Additional sections discuss Optogenetic control of lipid-modifying enzyme activity using LOV domain insertions, Chemoproteomics approaches for inhibition of diacylglycerol kinases, Inhibition of sterol transfer protein function, Preparation of K-Ras4B containing synthetic modifications via expressed protein ligation, Regulation of lipin enzymes on membranes, Use of novel synthetic analogues to decipher the interactions of phosphatidic acids species, Analyzing lipid-nuclear receptor interactions, and much more.

    • Molecular Biology Techniques

      A Classroom Laboratory Manual
      • 5th Edition
      • Sue Carson + 3 more
      • English
      Molecular Biology Techniques: A Classroom Laboratory Manual, Fifth Edition, is an in-depth laboratory manual for upper-level undergraduates and beginning graduate students in the general field of molecular biology techniques and biochemical applications. Presented as a series of coordinated experiments that mimics either a one- or two-semester research experience, the book also provides theories and explanations behind the techniques. Topics include manipulation of recombinant DNA, protein expression and purification, analysis of mRNA levels, mammalian cell culture techniques, CRISPR-mediated gene knockdown, and more. New topics, such as Gibson assembly experiments, have also been included. For instructors, the book contains easy to follow guides and preparation lists, designed to facilitate lab setup and pre-lab questions for use as a teaching tool.

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