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Books in General microscopy

  • Microscopy in the Life Sciences from 1650 to the Present

    • 1st Edition
    • Joel B. Sheffield
    • English
    Microscopy in the Life Sciences from 1650 to the Present explores the transformation of the microscope and its applications and techniques over the course of history, illustrating how progress in optics and microscope design has informed our growing knowledge of cellular life. Beginning with early observations of cellular structure, the book tracks the movement towards understanding of cell biology and pathology, considering the changing landscape of microscopy, lens development, and limitations and challenges tackled. Users will also find content on recent advances, including fluorescence studies and the use of computational procedures to assemble images of individual molecules. This book traces the evolution of understanding that scientists have had regarding cellular life and how comprehension of this has been supported by the development of the microscope. It considers a range of experts central to its progression over the years, including Schleiden, Schwann, Abbe, Schott and Zeiss, and is a useful reference for researchers working with microscopy techniques across the biological sciences.
  • Advances in Optical and Electron Microscopy

    • 1st Edition
    • Volume 11
    • T Mulvey + 1 more
    • English
    Advances in Optical and Electron Microscopy, Volume 11 compiles papers on the important developments in optical and electron microscopy. This book discusses the instrumentation and operation for high-resolution electron microscopy; diffraction pattern and camera length; and electron microscopy of surface structure. The history of surface imaging by conventional transmission electron microscopy; ion probe microscopy; and secondary ion mass spectrometry with high lateral resolution are also elaborated. This text likewise covers the acoustic microscopy; quantitative methods; biological applications and near-surface imaging of solids; and interior imaging. This publication is a beneficial to students and individuals researching on optical and electron microscopy.
  • Visualizing RNA Dynamics in the Cell

    • 1st Edition
    • Volume 572
    • English
    Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe.
  • The Ore Minerals Under the Microscope

    An Optical Guide
    • 2nd Edition
    • Volume 3
    • Bernhard Pracejus
    • English
    The Ore Minerals Under the Microscope: An Optical Guide, Second Edition, is a very detailed color atlas for ore/opaque minerals (ore microscopy), with a main emphasis on name and synonyms, short descriptions, mineral groups, chemical compositions, information on major formation environments, optical data, reflection color/shade comparison with four common/standard minerals of a similar color or grey shade, and up to five high-quality photos for each mineral with scale. In addition, the atlas contains a compilation from some of the prominent publications in the field of ore microscopy presented on a list of 431 minerals.
  • Immunocytochemistry

    Practical Applications in Pathology and Biology
    • 1st Edition
    • Julia M. Polak + 1 more
    • English
    Immunocytochemistry: Practical Applications in Pathology and Biology focuses on the processes, principles, methodologies, and approaches involved in the applications of immunocytochemistry in pathology and biology. The selection first takes a look at the development and scope of immunocytochemistry; techniques and practice used in immunocytochemistry; and raising and testing antibodies for immunocytochemistry. The book then ponders on raising antibodies to small peptides, use of semithin frozen sections in immunocytochemistry, and colloidal gold probes in immunocytochemistry. Discussions focus on the production of gold probes, localization of mouse serum albumin in the mammary gland, immunogens and the immune response, production of antisera, and antibody specificity in immunocytochemistry. The publication takes a look at double immunoenzymatic labelling, lectin histochemistry, immunocytochemical localization of noradrenaline, adrenaline, and serotonin, and immunocytochemistry of cell and tissue cultures. Immunocytochemistry in endocrine pathology and indirect immunofluorescence in the study and diagnosis of organ-specific autoimmune diseases are also discussed. The selection is a dependable source of information for researchers interested the practical applications of immunocytochemistry in pathology and biology.
  • Autoradiography and Immunocytochemistry

    Practical Methods in Electron Microscopy
    • 1st Edition
    • M.A. Williams
    • Audrey. M. Glauert
    • English
    Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining” with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labele... tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
  • Urine Analysis

    • 1st Edition
    • Byomkes Das Gupta
    • English
    Urine Analysis presents the theory and methods of urine analysis, which serves as a guide for physicians and medical laboratory technicians. Topics discussed in the book include the physical examination of urine; its chemical examination; microscopical examination; and Ehrliches Diazo reaction. Physicians and medical laboratory technicians will find the book a very valuable reference material.
  • Progress in Microscopy

    International Series of Monographs on Pure and Applied Biology: Modern Trends in Physiological Sciences
    • 1st Edition
    • M. Françon
    • P. Alexander + 1 more
    • English
    Progress in Microscopy details the advancement in various areas of microscopy. The title covers the phenomena, techniques, measurements, and equations. The text first details the physical aspects of image formation in microscopy, and then proceeds to tackling phase contrast, interference, and reflected-light microscopy. Next, the selection deals with the geometrical measurements and the measurement of refraction indices, thickness, and slope. The text also covers infra-red and ultra-violet microscopy, microspectroscopy, microspectrophotomet... and chemical microscopy. The book will be of great use to physicists who specializes in optics.
  • Practical Biology

    For Advanced Level, Medical and Intermediate Students
    • 5th Edition
    • C. J. Wallis
    • English
    Practical Biology for Advanced Level and Intermediate Students, Fifth Edition is an eight-part laboratory manual covering the syllabuses in biology of the advanced level students and other examinations of similar standard. The Introduction presents general instructions for practical work and for the keeping of practical notebooks and a list of apparatus and instruments required, as well as a summary of the characteristics of living organisms, the differences between plants and animals and the principles of plant classification. Part I describes first the features and uses of a microscope, followed by a presentation of guidelines for the preparation of microscopical slides. Parts II to IV are devoted to the evaluation of the form, structure, the microscopical structure of tissues and organs, and the very important aspect of their mode of functioning. Parts V to VIII explore the biochemical, embryological, and genetic aspects of life. These parts also consider other forms and modes of life, including insectivorous plants, fungi, bacteria, saprophytism, symbiosis, commensalism, and parasitism. This book is directed toward advanced and intermediate level botany teachers and students.
  • Optical Microscopy

    Emerging Methods and Applications
    • 1st Edition
    • Brian Herman + 1 more
    • English
    Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances
  • STM and SFM in Biology

    • 1st Edition
    • Othmar Marti + 1 more
    • English
    STM and SFM in Biology is a book fully dedicated to biological applications of the new technology of scanning probe microscopy (SX). The scanning tunneling microscope (STM) and its first off-spring, the scanning force microscope (SFM), resolve surface topography at the atomic scale. They also detect certain electronic and mechanical properties, and perform well in ultrahigh vacuum, ambient atmosphere, and aqueous solution environments. Thus, STM and SFM offer powerful tools for biological investigations of nucleic acids, proteins, membranes, and living cells.
  • Digital Microscopy

    • 3rd Edition
    • Volume 114
    • Greenfield Sluder + 1 more
    • English
    The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
  • Digital Microscopy

    A second edition of "Video Microscopy"
    • 2nd Edition
    • Volume 72
    • English
    This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half.
  • Advances in Microbial Physiology

    • 1st Edition
    • Volume 43
    • English
    TOPICS COVERED IN THIS VOLUME1. The molecular genetics of cultivated mushrooms2. The intestinal microflora: potentially fertile ground for microbial physiologists3. Primary metabolism and its control in Streptomycetes: a most unusual group of bacteria4. Acid adaptation in oral Stretococci
  • Green Fluorescent Proteins

    • 1st Edition
    • Volume 58
    • English
    This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.
  • Video Microscopy

    • 1st Edition
    • Volume 56
    • English
    Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques.
  • Cell Biological Applications of Confocal Microscopy

    • 1st Edition
    • Volume 38
    • English
    Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.
  • Cytochemical Staining Methods for Electron Microscopy

    • 1st Edition
    • P.R. Lewis + 1 more
    • English
    The series Practical Methods in Electron Microscopy, edited by Audrey M. Glauert has an international reputation asa unique source of practical information for all electron microscopists.Each book of the series starts from first principles, assuming nospecialist knowledge, and is complete in itself. The series willeventually cover the whole range of techniques for electron microscopy.In this latest volume in the popular series, Peter Lewis and David Knight describe in practical detail the whole range of staining techniques for electron microscopy, including contrast staining for ultrastructural studies, specific cytological staining methods, and enzyme cytochemical techniques.The chemical basis of cytochemical methods is discussed and analysed in a straightforward manner with great clarity. In consequence this book will help the beginner to choose between the sometimes bewildering variety of published technical procedures.Electron microscopists will find this volume to be an invaluable and compact source-book for all those cytochemical techniques that have proved to be both reliable and convenient to use. As with previous titles in this popular series, the unique type of practical guidance provided will be of value for many years to come.