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Correlative Light and Electron Microscopy III
1st Edition - May 23, 2017
Editors: Thomas Muller-Reichert, Paul Verkade
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Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters… Read more
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Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics.
Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.
Contains contributions from experts in the field
Covered topics include targeted ultramicrotomy and high-precision correlation
Presents recent advances and currently applied correlative approaches
Gives detailed protocols allowing the application of workflows in one’s own laboratory setting
Covers CLEM approaches in the context of specific applications
Aims to stimulate the use of new combinations of imaging modalities
Researchers and students in cell, molecular and developmental biology
1. Millisecond time-resolved CLEMGaia Pigino2. Super resolution LM und SEM of high-pressure frozen C. elegansChristian Stigloher3. Preservation fluorescence, super res CLEMErrin Johnson4. Targeted ultramicrotomyYannick Schwab5. CEMOVIS-CLEMYannick Schwab6. APEX in TissueRobert G. Parton7. Corrsight mit IBIDI flowthrough chamberMiriam Susanna Lucas8. CorrsightClaudia S. Lopez9. Correlative Light Atomic Force Electronic Microscopy (CLAFEM)Frank Lafont10. Atmospheric EM CLEMChikira Sato11. Correlated Fluo Light SEMFilip Braet12. Near infrared branding + SBF SEMPaul Verkade13. 4. Correlative MicroCTMatthia Andrea Karreman14. High-precision correlationWanda Kukulski15. CLEM acquisitionMartin Schorb and Frank Sieckmann 16. ecCLEM mapping softwareXavier Heiligenstein and Perrine Paul-Gilloteaux
No. of pages: 370
Published: May 23, 2017
Imprint: Academic Press
Hardback ISBN: 9780128099759
eBook ISBN: 9780128099766
Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.
TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.
He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy.
Affiliations and expertise
Professor of Structural Cell Biology, Technische Universitat Dresden, TU Dresden, Germany
Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.