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Books in Life sciences

  • Immunocytochemistry

    Practical Applications in Pathology and Biology
    • 1st Edition
    • Julia M. Polak + 1 more
    • English
    Immunocytochemistry: Practical Applications in Pathology and Biology focuses on the processes, principles, methodologies, and approaches involved in the applications of immunocytochemistry in pathology and biology. The selection first takes a look at the development and scope of immunocytochemistry; techniques and practice used in immunocytochemistry; and raising and testing antibodies for immunocytochemistry. The book then ponders on raising antibodies to small peptides, use of semithin frozen sections in immunocytochemistry, and colloidal gold probes in immunocytochemistry. Discussions focus on the production of gold probes, localization of mouse serum albumin in the mammary gland, immunogens and the immune response, production of antisera, and antibody specificity in immunocytochemistry. The publication takes a look at double immunoenzymatic labelling, lectin histochemistry, immunocytochemical localization of noradrenaline, adrenaline, and serotonin, and immunocytochemistry of cell and tissue cultures. Immunocytochemistry in endocrine pathology and indirect immunofluorescence in the study and diagnosis of organ-specific autoimmune diseases are also discussed. The selection is a dependable source of information for researchers interested the practical applications of immunocytochemistry in pathology and biology.

  • Glutathione

    Proceedings of the Symposium Held at Ridgefield, Connecticut, November, 1953
    • 1st Edition
    • S. Colowick + 2 more
    • English
    Glutathione: A Symposium covers the proceedings of the symposium held in Ridgefield, Connecticut in November 1953. The book focuses on the chemistry, characteristics, reactions, and properties of glutathione (GSH). The selection first offers information on mercaptans and disulfides, reactivity of the sulfhydryl group in GSH and related peptides, and chemistry and properties of GSH. Discussions focus on isolation and occurrence of GSH, properties of GSH, general chemistry of acidity, oxidation, and disulfides, and application to the structure of GSH. The text then examines thiol-thiolester complex formation, methods of GSH assay, and paper chromatography of GSH and its hydrolysis products. The publication takes a look at histochemical experiments on sulfhydryls and disulfides, enzymatic oxidation and reduction of GSH, and biosynthesis of GSH. Topics include synthesis of GSH from the constituent amino acids, enzymatic oxidation of GSH, and methods for demonstrating protein-bound sulfhydryl and disulfide groups in tissues. The selection is a dependable reference for researchers interested in the chemistry, reactions, and properties of glutathione.

  • Oxygenases

    • 1st Edition
    • Osamu Hayaishi
    • English
    Oxygenases focuses on the processes, methodologies, technologies, and approaches involved in oxygenases, including its distribution in animals, plants, and microorganisms and its role in the metabolism of aromatic, cyclic, and aliphatic compounds. The selection first offers information on the history and scope of oxygenases, methodology of oxygen isotopes, and phenolytic oxygenases. Discussions focus on the separation and purification of compounds for isotopic analysis; general properties and mechanisms of action of oxygenases; physiological significance of oxygenases; dehydrogenases, oxidases, and oxygenases; and problems and restrictions in the use of oxygen-18. The text then examines aromatic hydroxylations, oxygenases in lipid and steroid metabolism, and bacterial oxidation of hydrocarbons. Topics include anaerobic oxidation of alkanes, involvement of molecular oxygen in hydrocarbon utilization, steroid hydroxylation mechanisms, oxygenases in the biosynthesis of steroid hormones, conversion of dopamine to norepinephrine, and general discussion of mechanism of aerobic hydroxylation reactions. The selection is a valuable reference for researchers interested in the process of oxygenases.

  • Gel Electrophoresis of Proteins

    • 1st Edition
    • Michael J Dunn
    • English
    Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoreti... methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.

  • Biophysical Chemistry

    Thermodynamics, Electrostatics, and the Biological Significance of the Properties of Matter
    • 1st Edition
    • John T. Edsall + 1 more
    • English
    Biophysical Chemistry, Volume I: Thermodynamics, Electrostatics, and the Biological Significance of the Properties of Matter focuses on the biological aspects of the properties of matter, putting emphasis on the chemical elements, water and carbon dioxide, complex molecules, and proteins. The publication first elaborates on biochemistry and geochemistry, water and its biological significance, and the problems of protein structure. Discussions focus on the number of peptide chains in the molecule and nature of terminal groups, latent heat of fusion, characteristics of the amino acids derived from proteins, expansion of water in freezing, and the relative abundance of chemical elements in the universe. The text then takes a look at thermodynamics and the application to polar molecules and ionic solutions of electrostatics, including free energy of a charged sphere, image charges, salting-out effect, expressions for the change of fundamental thermodynamic functions, and chemical potentials. The book examines the conductivity of electrolytes, acid-base equilibria, and polybasic acids, bases, and ampholytes, including proteins. Topics include ionization of cysteine, isoelectric points of polyvalent ampholytes, hemoglobin, nature of acids and bases, measurement of conductivity, electrolytes as conductors, and the moving boundary method of determining transference numbers. The manuscript is a dependable reference for chemists and researchers interested in thermodynamics, electrostatics, and the biological value of the properties of matter.

  • Carbohydrate Metabolism

    And Its Disorders
    • 1st Edition
    • F. Dickens + 2 more
    • English
    Carbohydrate Metabolism and its Disorders focuses on the processes and methodologies involved in carbohydrate metabolism, including detection of diabetes, hypoglycemic syndromes, cardiovascular diseases, and atherosclerosis. The selection first takes a look at the detection of diabetes in man, hormonal disturbances in diabetes, and hypoglycemia. Topics include indications for testing for diabetes, reasons for early detection, testing for diabetes with tolbutamide, glucagon, corticosteroids, and hypoglycemic syndromes. The book also ponders on the physiological aspects of carbohydrate metabolism in the fetus and newborn and glycogen-storage diseases. The publication examines dietary intake of carbohydrate in relation to diabetes and atherosclerosis and glucose tolerance in ischemic cardiovascular disease. Discussions focus on epidemiological studies of diabetes and atherosclerosis, overt diabetes mellitus, and effects of selection, age, obesity, and other factors. The book also identifies disturbances of the digestion and absorption of carbohydrates; insulin antagonists and disturbances in carbohydrate metabolism; and glycosaminoglycans in joint disorders. The selection is a valuable reference for readers interested in carbohydrate metabolism.

  • Biochemistry of Lipids

    Biochemistry Series One
    • 1st Edition
    • T. W. Goodwin
    • English
    MTP International Review of Science: Biochemistry Series One, Volume 4: Biochemistry of Lipids focuses on the processes, methodologies, reactions, and approaches involved in the biochemistry of lipids. The selection first elaborates on the enzymes of sterol biosynthesis and lipids in glycan biosynthesis. Topics cover polyprenols and glycan biosynthesis in green plants; polyprenols and glycan biosynthesis in yeasts and fungi; undecaprenol and bacterial wall glycan biosynthesis; methods of investigating particle-bound enzymes of sterol biosynthesis; and relationship of the multi-enzymic synthesis of cholesterol to other microsomal processes. The text then elaborates on the biosynthesis of saturated fatty acids, dynamic role of lipids in the nervous system, and biosynthesis of unsaturated fatty acids. Discussions focus on anaerobic pathway of monoenoic fatty acid biosynthesis, exchange of brain lipids, lipids and transmission in the nervous system, fatty acid synthetase, and lipid distribution within the nervous system. The manuscript examines halogenated sulphatides and prostaglandins, including identification of prostaglandins in organs and body fluids, estimation of prostaglandins, and occurrence of halosulphatides in membranes. The selection is a valuable reference for researchers interested in the biochemistry of lipids.

  • Polysaccharides Peptides and Proteins

    Pharmaceutical Monographs
    • 1st Edition
    • R. T. Coutts + 1 more
    • J. B. Stenlake
    • English
    Pharmaceutical Monographs, Volume 4: Polysaccharides Peptides and Proteins provides information pertinent to a wide variety of naturally-occurring macromolecules that are either peptide, protein, or polysaccharide in nature, which are important as medicinal and pharmaceutical agents. This monograph presents the basic physical and chemical properties of polysaccharides. Organized into four chapters, this volume begins with an overview of the pharmaceutical importance of the class of compounds known as polysaccharides. This text then provides data on the structure of the polysaccharides that is obtained by isolating and identifying the product formed on hydrolysis of the molecule itself. Other chapters consider plant gums and mucilages as important medicinal and pharmaceutical products. This monograph discusses as well the autohydrolysis of gum arabic. The final chapter deals with the clinical applications of various peptide-type antibiotics. This monograph is a valuable resource for undergraduate students of pharmacy and allied subjects.

  • Metabolic Pathways

    Second Edition of Chemical Pathways of Metabolism
    • 2nd Edition
    • David M. Greenberg
    • English
    Metabolic Pathways, Volume II focuses on the metabolism, biosynthesis, and catabolism of amino acids. The selection first offers information on nitrogen and carbon metabolism of amino acids. Discussions focus on amino acids linked with citric acid cycle, sulfur amino acids, proline and hydroxyproline, histidine, hydroxyamino acids, urea biosynthesis and related systems, and deamidation. The text then ponders on the biosynthesis of amino acids and related compounds and metabolism of sulfur-containing compounds. Topics include metabolism of methionine, biotin, biological importance of sulfur in animals, interconversions of glutamic acid, ornithine, and prolines, and biosynthesis of the branched-chain amino acids. The publication takes a look at the synthesis of proteins, purines and pyrimidines, and nucleotides and nucleosides, including the components of nucleotides, purine degradation, and incorporation of preformed purine compounds into nucleic acids. The selection is a valuable reference for researchers interested in the metabolism, catabolism, and biosynthesis of amino acids.

  • Essentials of ABO -Rh Grouping and Compatibility Testing

    Theoretical Aspects and Practical Application
    • 1st Edition
    • W. John Lockyer
    • English
    Essentials of ABO-Rh Grouping and Compatibility Testing: Theoretical Aspects and Practical Application focuses on overall safety in blood transfusion, including accurate ABO and Rh-D grouping of both patient and donor. The book first elaborates on the ABO blood group system and the Rh blood group system and hemolytic disease of the newborn. Discussions focus on the Rh-D antigen, Rh genotyping, Rh testing of blood donors and recipients of blood, antenatal and postnatal serology in cases other than Rh and ABO hemolytic disease, selection and preparation of Rh anti-D grouping reagent, dangerous group O donor blood, alternative sources of anti-A and anti-B blood grouping reagent, and development of the A and B red cell antigens. The manuscript then takes a look at compatibility testing (crossmatching) and transfusion reaction investigations, as well as compatibility testing and preparation of blood for patients with antibodies to serum proteins; compatibility testing of patients with known irregular antibodies; non-urgent compatibility testing of blood; and compatibility testing for patients requiring platelets. The publication takes a look at practical procedures, including grades of agglutination, control of anti-human globulin serum, preparation of low ionic strength saline, and preparation of platelet concentrates. The text is a valuable source of information for researchers interested in ABO-Rh grouping and compatibility testing.

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