Life sciences print books and ebooks | Elsevier

Immunological Methods

1st Edition
May 12, 2014
Ivan Lefkovits + 1 more
English
Paperback
9 7 8 1 4 8 3 2 4 2 5 3 8
eBook
9 7 8 1 4 8 3 2 6 5 3 6 0

Immunological Methods, Volume IV provides information pertinent to the methods in immunological research. This book focuses on cells, clones, and cell lines, as well as on their components and secreted products. Organized into 21 chapters, this volume begins with an overview of hybridoma methodology as the most celebrated immunological method. This text then discusses cell fusion, hybridoma technology, and everything related to monoclonal antibodies. Other chapters consider another molecular biology method, which describes the procedure required for establishing a partitioned cDNA-library. This book provides as well a comprehensive analysis of mRNA populations in which every messenger species appears as a distinct element, and so provides accurate answers to questions concerning genetic complexity. The final chapter provides an example of how transgenic mice can be used to study the development of T cell repertoires. This book is a valuable resource for cell biologists, scientists, immunologists, and research workers.

The Plasma Proteins

1st Edition
May 12, 2014
Frank W Putnam
English
Paperback
9 7 8 1 4 8 3 2 5 1 3 5 6
eBook
9 7 8 1 4 8 3 2 5 8 6 7 6

The Plasma Proteins, Volume I: Isolation, Characterization, and Function focuses on the reactions, properties, characteristics, and transformations of plasma proteins. The selection first offers information on the fractionation and isolation of purified components by precipitation methods and electrophoretic and ultracentrifugal analysis of normal human serum. Discussions focus on correlation of electrophoretic and ultracentrifugal results, electrophoretic analytical methodology and results, parameters influencing protein solubility, and techniques for the separation of proteins by precipitation methods. The text then ponders on the chromatography of plasma proteins and chemical composition and molecular parameters of purified plasma proteins. The manuscript elaborates on plasma albumin and macroglobulins and high molecular weight antibodies. Topics include immunological properties, physical and chemical properties of normal and pathological macroglobulins, purity, homogeneity, and variability, denaturation behavior, and sulfhydryl groups, mercaptalbumin, and the mercury dimer. The book then examines glycoproteins and metal-binding plasma proteins and cation transport. The selection is a highly recommended reference for biochemists and clinicians interested in plasma proteins.

Chemical Pathways of Metabolism

1st Edition
May 12, 2014
David M. Greenberg
English
Paperback
9 7 8 1 4 8 3 2 5 4 7 3 9
eBook
9 7 8 1 4 8 3 2 7 4 2 8 7

Chemical Pathways of Metabolism, Volume II focuses on the chemical processes involved in the metabolism of the essential components of living organisms, including catabolism, deamination, bonds, and synthetic processes. The selection first elaborates on nitrogen metabolism and carbon catabolism of amino acids, including deamination, urea synthesis, amino acids linked with the citric acid cycle, sulfur amino acids, and arginine and ornithine. The book then ponders on the synthetic processes involving amino acids and metabolism of sulfur-containing compounds. Discussions focus on reactions of sulfur-containing coenzymes, relationships of methionine and cysteine, desulfhydrase reaction, formation of phosphatide bases, and interconversions of glutamic acid, ornithine, and proline. The manuscript takes a look at the enzymatic syntheses of peptide bonds, purines and pyrimidines, and nucleotides and nucleosides. Topics include enzymatic splitting of coenzyme nucleotides, deamination of nucleosides and nucleotides, enzymatic synthesis of coenzyme nucleotides, purines, and pyrimidines. The selection is a valuable source of information for researchers interested in the chemical pathways of metabolism.

Protein and Amino Acid Requirements of Mammals

1st Edition
May 12, 2014
Anthony A. Albanese
English
Paperback
9 7 8 1 4 8 3 2 5 5 8 0 4
eBook
9 7 8 1 4 8 3 2 6 7 6 6 1

Protein and Amino Acid Requirements of Mammals focuses on the determination of the protein and amino acid requirements of various mammals, including man in particular. The selection first offers information on some species and age differences in amino acid requirements and the methods of measuring the nutritive value of protein hydrolysates and amino acid mixtures. Discussions focus on assay methods based on protein regeneration; specificity of amino acid requirements; amino acid requirements for nitrogen equilibrium in the adult; and composition of tissue proteins and amino acid requirements for growth. The text then examines the laboratory evaluation of amino acid mixtures and protein hydrolysates and dietary proteins and synthesis of tissue proteins. Topics include role of dietary proteins in the hypertrophy of gonads, effect of dietary proteins on repletion of liver proteins, and effect of dietary proteins on the synthesis of plasma proteins. The publication examines the protein and amino acid requirements of man, including qualitative amino acid requirements of various species, growth and protein and amino acid needs, and metabolic interactions of essential and nonessential amino acids. The selection is a dependable reference for researchers interested in the determination of the protein and amino acid requirements of mammals, including man.

Gel Electrophoresis of Proteins

1st Edition
May 12, 2014
Michael J Dunn
English
Paperback
9 7 8 1 4 8 3 1 7 7 7 3 1
eBook
9 7 8 1 4 8 3 1 9 3 5 4 0

Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoreti... methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.

The Peptides

1st Edition
May 12, 2014
Eberhard Schröder + 1 more
English
eBook
9 7 8 1 4 8 3 2 5 9 8 6 4

The Peptides, Volume II: Synthesis, Occurrence, and Action of Biologically Active Polypeptides focuses on the synthesis of biologically active polypeptides and analogues. The publication first offers information on linear peptides and heterodetic cyclic peptides. Discussions focus on depsipeptides, polycyclic and monocyclic disulfides, naturally occurring biologically active peptides, glandular hormones, and tissue hormones. The text then ponders on homodetic cyclic peptides, including homodetic cyclic homomeric peptide antibiotics, polycyclic biologically active polypeptides, and homodetic cyclic heteromeric peptide antibiotics. The manuscript takes a look at the problems related with the synthesis of biologically active polypeptides, giving emphasis to a number of movements which elaborated on the matter and the advancements of preparative techniques and approaches leading to the purification of intermediates and end products. The publication is a valuable reference for researchers interested in the occurrence, synthesis, and action of biologically active polypeptides.

Fertilization

1st Edition
May 12, 2014
Charles B. Metz + 1 more
English
Paperback
9 7 8 1 4 8 3 2 5 1 0 1 1
eBook
9 7 8 1 4 8 3 2 5 8 4 3 0

Fertilization: Comparative Morphology, Biochemistry, and Immunology, Volume I focuses on the many aspects of the physiological mechanisms of fertilization in different forms of life. The selection first tackles the problems and procedures of comparative gametology and syngamy and sperm motility. Discussions focus on the morphology of cilia and flagella, extraneous chemical and physical factors, motility in male and female reproductive tests, problems of the quantitative and qualitative control of fertility, and patterns of reproduction. The text then ponders on sperm metabolism and mechanisms of gametic approach in plants. The manuscript takes a look at gamete surface components and their role in fertilization, acrosome reaction and lysins, and membrane fusion in relation to sperm-egg association. Topics include gametic association, acrosome formation during spermiogenesis, fine-structure studies of acrosomal changes, specificity of fertilization, and amphibian fertilization. The book also examines the activation of the egg and parthenogenesis in vertebrates. The selection is a valuable reference for readers interested in the processes involved in fertilization.

Environmental Regulation of Microbial Metabolism

1st Edition
May 12, 2014
I. S. Kulaev + 2 more
English
eBook
9 7 8 1 4 8 3 2 7 2 4 9 8

Environmental Regulation of Microbial Metabolism contains the proceedings of the Federation of European Microbiological Societies Symposium held in Pushchino, USSR, on June 1-7, 1983. The symposium provided a forum for reviewing the environmental regulation of the metabolism of autotrophic and heterotrophic microorganisms; regulation of the biogenesis of cell walls and other structures in prokaryotic and eukaryotic microorganisms; and regulation of the secretion of macromolecules and transport of ions and solutes across microbial membranes. Comprised of 52 chapters, this book begins with an overview of some aspects of environmental regulation of microbial phosphorus metabolism, followed by a discussion on the role of covalent interconversion of enzymes in the regulation of microbial metabolism. Subsequent chapters explore the role and regulation of inorganic pyrophosphatase in bacteria; the central metabolism of pseudomonads degrading xenobiotics; the role of nickel enzymes in anaerobic metabolism; and the oxidation of aromatic compounds by microalgae. The book also considers carbon metabolism in phototrophic bacteria under different conditions of growth, along with the transport of solutes and metabolites in prokaryotes and eukaryotes. This monograph will be helpful to a wide range of specialists in the fields of biochemistry, physiology, molecular biology, and biotechnology.

Immunocytochemistry

1st Edition
May 12, 2014
Julia M. Polak + 1 more
English
Paperback
9 7 8 1 4 8 3 1 7 7 6 5 6
eBook
9 7 8 1 4 8 3 1 9 3 4 6 5

Immunocytochemistry: Practical Applications in Pathology and Biology focuses on the processes, principles, methodologies, and approaches involved in the applications of immunocytochemistry in pathology and biology. The selection first takes a look at the development and scope of immunocytochemistry; techniques and practice used in immunocytochemistry; and raising and testing antibodies for immunocytochemistry. The book then ponders on raising antibodies to small peptides, use of semithin frozen sections in immunocytochemistry, and colloidal gold probes in immunocytochemistry. Discussions focus on the production of gold probes, localization of mouse serum albumin in the mammary gland, immunogens and the immune response, production of antisera, and antibody specificity in immunocytochemistry. The publication takes a look at double immunoenzymatic labelling, lectin histochemistry, immunocytochemical localization of noradrenaline, adrenaline, and serotonin, and immunocytochemistry of cell and tissue cultures. Immunocytochemistry in endocrine pathology and indirect immunofluorescence in the study and diagnosis of organ-specific autoimmune diseases are also discussed. The selection is a dependable source of information for researchers interested the practical applications of immunocytochemistry in pathology and biology.

Mammalian Protein Metabolism

1st Edition
May 12, 2014
H. N. Munro + 1 more
English
eBook
9 7 8 1 4 8 3 2 7 2 9 1 7

Mammalian Protein Metabolism, Volume II focuses on the processes, methodologies, and reactions involved in mammalian protein metabolism, including toxicities, amino acid imbalance, and digestion and absorption. The selection first offers information on the nutritive value of dietary proteins, amino acid toxicities and imbalances, and protein requirements. Discussions focus on the protein requirements of adults, infants, and children, amino acid requirements, protein requirements in pregnancy, amino acid imbalance, mixed diets, and nutritive value and maintenance. The text then ponders on protein metabolism and requirements in pregnancy and lactation and protein metabolism and requirements in the newborn. The publication examines protein metabolism and requirements in the elderly; anomalies of amino acid metabolism; and physical injury and its effects on protein metabolism. Topics include general disturbance of protein metabolism following physical injury, nature of inflammatory reaction, acquired disturbances of amino acid metabolism, plasma proteins, nitrogen balance, digestion and absorption, and protein withdrawal. The text then elaborates on protein metabolism and tumor growth, experimental protein-calorie deficiency, and the clinical aspects of protein malnutrition. The selection is a dependable source of data for researchers interested in mammalian protein metabolism.

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