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Elsevier

Books in Chemistry

Chemistry topic areas include: physical and theoretical, computational, organic, organometallic and inorganic, pharmaceutical and medicinal, analytical and bioanalytical, nuclear, general, nanochemistry, geochemistry, materials and polymer, as well as environmental, green and sustainable chemistry.

  • Modified Nucleosides in Cancer and Normal Metabolism - Methods and Applications

    • 1st Edition
    • Volume 45C
    • English

  • Sol-Gel Science

    The Physics and Chemistry of Sol-Gel Processing
    • 1st Edition
    • C. Jeffrey Brinker + 1 more
    • English
    Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing presents the physical and chemical principles of the sol-gel process. The book emphasizes the science behind sol-gel processing with a chapter devoted to applications. The first chapter introduces basic terminology, provides a brief historical sketch, and identifies some excellent texts for background reading. Chapters 2 and 3 discuss the mechanisms of hydrolysis and condensation for nonsilicate and silicate systems. Chapter 4 deals with stabilization and gelation of sols. Chapter 5 reviews theories of gelation and examines the predicted and observed changes in the properties of a sol in the vicinity of the gel point. Chapter 6 describes the changes in structure and properties that occur during aging of a gel in its pore liquor (or some other liquid). The discussion of drying is divided into two parts, with the theory concentrated in Chapter 7 and the phenomenology in Chapter 8. The structure of dried gels is explored in Chapter 9. Chapter 10 shows the possibility of using the gel as a substrate for chemical reactions or of modifying the bulk composition of the resulting ceramic by performing a surface reaction (such as nitridation) on the gel. Chapter 11 reviews the theory and practice of sintering, describing the mechanisms that govern densification of amorphous and crystalline materials, and showing the advantages of avoiding crystallization before sintering is complete. The properties of gel-derived and conventional ceramics are discussed in Chapter 12. The preparation of films is such an important aspect of sol-gel technology that the fundamentals of film formation are treated at length in Chapter 13. Films and other applications are briefly reviewed in Chapter 14. Materials scientists and researchers in the field of sol-gel processing will find the book invaluable.

  • The Alkaloids: Antitumor Bisindole Alkaloids from Catharanthus roseus (L.)

    • 1st Edition
    • Volume 37
    • Arnold Brossi + 1 more
    • English

  • Immobilized pH Gradients: Theory and Methodology

    • 1st Edition
    • Volume 20
    • Pier Giorgio Righetti
    • English
    Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.

  • PCs for Chemists

    • 1st Edition
    • Volume 5
    • J. Zupan
    • English
    The book first introduces the reader to the fundamentals of experimental design. Systems theory, response surface concepts, and basic statistics serve as a basis for the further development of matrix least squares and hypothesis testing. The effects of different experimental designs and different models on the variance-covariance matrix and on the analysis of variance (ANOVA) are extensively discussed. Applications and advanced topics (such as confidence bands, rotatability, and confounding) complete the text. Numerous worked examples are presented. The clear and practical approach adopted by the authors makes the book applicable to a wide audience. It will appeal particularly to those with a practical need (scientists, engineers, managers, research workers) who have completed their formal education but who still need to know efficient ways of carrying out experiments. It will also be an ideal text for advanced undergraduate and graduate students following courses in chemometrics, data acquisition and treatment, and design of experiments.

  • Advances in Food and Nutrition Research

    • 1st Edition
    • Volume 33
    • English

  • Profiles of Drug Substances, Excipients and Related Methodology

    • 1st Edition
    • Volume 18
    • English

  • Annual Reports on NMR Spectroscopy

    • 1st Edition
    • Volume 22
    • English

  • Advances in Inorganic Chemistry

    • 1st Edition
    • Volume 34
    • English

  • Advances in Carbohydrate Chemistry and Biochemistry

    • 1st Edition
    • Volume 47
    • English

  • The Alkaloids: Chemistry and Pharmacology

    • 1st Edition
    • Volume 36
    • English

  • Analytical Methods for Major and Modified Nucleosides - HPLC, GC, MS, NMR, UV and FT-IR

    • 1st Edition
    • Volume 45A
    • English

  • Biological Roles and Function of Modification

    • 1st Edition
    • Volume 45B
    • English

  • Optical Techniques to Characterize Polymer Systems

    • 1st Edition
    • H. Bässler
    • English
    To optimize the properties of polymers or to design new polymeric materials for special applications, one has to understand how their properties depend on chemical and physical structure. This, in turn, requires methods for material characterization operating on a microscopic level such as optical techniques. Over the past decades major progress has been made in exploiting the analytical power offered by spectroscopic methods if combined with modern light source and detection technology.This multi-author book has been written in order to acquaint a broader audience with these recent achievements. Its individual chapters have been arranged in three groups featuring absorption, luminescence and diffraction techniques including non-linear optics for probing the physical structure of polymers, as well as their dynamic behavior in the presence and absence of electronic excitation. Emphasis has been placed on recent developments in the spectroscopy of solid polymers.

  • Advances in Heterocyclic Chemistry

    • 1st Edition
    • Volume 46
    • English

  • Selective Sample Handling and Detection in High-Performance Liquid Chromatography

    • 1st Edition
    • Volume B
    • K. Zech + 1 more
    • English
    This is the second of a two-volume project which treats the handling, separation and detection of complex samples as an integrated, interconnected process. On the basis of this philosophy the editors have selected those contributions which demonstrate that optimal sample preparation leads to a simplification of detection or reduced demands on the separation process. Throughout the book emphasis is on chemical principles with minimum discussion of the equipment required - an approach which reflects the editors' view that the limiting factor in the analysis of complex samples is an incomplete knowledge of the underlying chemistry rather than the hardware available. This lack of knowledge becomes more evident as the demands for lower detection limits grow, as solving complex matrix problems requires a greater understanding of the chemical interaction between the substance to be analysed and the stationary phase. Thus, apart from one chapter dealing with chemically modified silicas, the main theme of the book is developed in three chapters on sample preparation and three on detection.The opening chapter outlines concentration and chromatography on chemically modified silicas with complexing properties, and gives examples of the use of these phases with organic and inorganic compounds. Chapter II, the first of the three contributions dealing with sample preparation, addresses such questions as whether the prepared sample is representative of the material to be analysed; how to avoid contamination; which separation procedure should be used to avoid tedious sample preparation. Chapter III describes the processing of whole blood for drug analysis. The determination of cyclosporine and its metabolites (an especially difficult case) demonstrates how comprehensive the optimisation of sample preparation must be to successfully perform the analysis. Several other examples are also given. Chapter IV deals with radio-column liquid chromatography and introduces the other theme of the book, i.e. selective detection methods. The widespread use of radioisotopes requires a high degree of purification during the manufacture of the compounds, as well as highly accurate detection methods in biological and biochemical studies.Chapter V continues the theme of selective detection with an overview of post-column reaction detection. The use of immobilised enzymes in post-column reactors or `pumpless' reactor systems for on-line reagent generation after the chromatographic separation step is discussed in detail. Various examples of the separation of biological compounds show how the production of electrochemical reagents and photochemical reaction detection have increased the selectivity of the detection, leading to more economical analytical systems. Selective detection employing luminescence detection techniques is outlined in Chapter VI. The use of immobilised fluorophores or the coupling to photochemical reactions leads to highly selective detection systems which can greatly simplify the sample handling. The final chapter reviews the use of continuous separation techniques in flow injection analysis thus revealing the need for a strong interdisciplinary dependence between sample handling and separation in this area.Written by experienced practitioners, this book will be extremely useful to investigators in many areas of application. Each chapter includes sufficient references to the literature to serve as a valuable starting point for more detailed investigation. The strong emphasis on sample handling makes the book unique in many ways and it will be welcomed by environmental scientists as well as those active in the clinical, pharmaceutical and bioanalytical fields.

  • Advances in Quantum Chemistry

    • 1st Edition
    • Volume 20
    • English

  • Advanced EPR

    Applications in Biology and Biochemistry
    • 1st Edition
    • A.J. Hoff
    • English
    Advanced EPR: Applications in Biology and Biochemistry provides an up-to-date survey of existing EPR techniques and their applications in biology and biochemistry, and also provides a wealth of ideas for future developments in instrumentation and theory. The material is broadly organized into four parts. In the first part (chapters 1 to 6) pulsed EPR is discussed in detail. The second part (chapters 7 to 12) provides detailed discussions of a number of novel and experimental methods. The third part comprises seven chapters on double-resonance techniques, five on ENDOR and two on optically- and reaction yield-detected resonance. The final part is devoted to a thorough discussion of a number of new developments in the application of EPR to various biological and biochemical problems. Advanced EPR will interest biophysicists, physical biochemists, EPR spectroscopists and others who will value the extensive treatment of pulsed EPR techniques, the discussion of new developments in EPR instrumentation, and the integration of theory and experimental details as applied to problems in biology and biochemistry.

  • The Alkaloids: Chemistry and Pharmacology

    • 1st Edition
    • Volume 35
    • English

  • Organic Functional Group Preparations

    Volume 3
    • 2nd Edition
    • Stanley R. Sandler + 1 more
    • English
    This volume provides organic chemists with a convenient, up-to-date reference source on reliable preparative procedures for the most common functional groups. Preparations for each functional group have been subdivided into various reaction types.

  • Flow Injection Analysis

    A Practical Guide
    • 1st Edition
    • Volume 10
    • B. Karlberg + 1 more
    • English
    This is a practical guide for first-time and experienced users of Flow Injection Analysis (FIA). It gives, not a detailed theoretical analysis, but a ``nuts and bolts'' approach to the description of the technique and how it can be utilized to solve analytical chemical problems.The advantages of flow injection, how, when, why and where it works are all fully explained. Criteria for the choice of hardware and useful hints for maintenance are provided. The large variety of detectors suitable to combine with FIA are discussed, as are special modes of operation, their advantages and their limitations, and also conversion of batch methods to FIA methods. Numerous in-depth descriptions of applications of FIA techniques in water, soil, pharmaceutical and industrial analysis are featured, and a complete bibliography is included.The authors have spent several years demonstrating, lecturing and using FIA and the basic outline of their book closely follows the schedule of the FIA workshops they have taught. It will be an invaluable tool for all chemists who perform analyses on a routine basis.

  • Reactions at the Liquid-Solid Interface

    • 1st Edition
    • Volume 28
    • R.G. Compton
    • English
    Volume 28 of Comprehensive Chemical Kinetics is concerned with reactions occurring at the solid-liquid interface. Volumes 26 and 27 have dealt with electrochemical reactions. The present volume therefore covers those interfacial processes which involve no net passage of current. The first chapter covers the structure of the interfacial region and its experimental investigation. The second chapter reviews the mechanisms by which heterogeneous catalysis of solution reactions can take place. The third is concerned with the mechanism and kinetics of crystal growth from solution. The final chapter deals with corrosion processes at the metal-solution interface.

  • Advances in Heterocyclic Chemistry

    • 1st Edition
    • Volume 45
    • English

  • Advances in Inorganic Chemistry

    • 1st Edition
    • Volume 33
    • English

  • Advances in Clinical Chemistry

    • 1st Edition
    • Volume 27
    • English

  • Photochemistry on Solid Surfaces

    • 1st Edition
    • Volume 47
    • Takeshi Matsuura + 1 more
    • English
    The latest developments in photochemistry on solid surfaces, i.e. photochemistry in heterogeneous systems, including liquid crystallines, are brought together for the first time in a single volume. Distinguished photochemists from various fields have contributed to the book which covers a number of important applications: molecular photo-devices for super-memory, photochemical vapor deposition to produce thin-layered electronic semiconducting materials, sensitive optical media, the control of photochemical reactions pathways, etc. Photochemistry on solid surfaces is now a major field and this book which provides an up-to-date and comprehensive overview of the subject will be of interest to a wide range of readers.

  • Advances in Organometallic Chemistry

    • 1st Edition
    • Volume 29
    • English

  • Annual Reports on NMR Spectroscopy

    • 1st Edition
    • Volume 21
    • English
    Commencing with Volume 21, the aim of this series is to produce a larger number of, relatively short but, timely and authoritative reviews. These will concentrate, in particular, on areas of NMR where recent progress, and developments, are of special interest and will reflect the many NMR developments occurring in the primary scientific literature. The present volume demonstrates the advances which can be made by the application of NMR techniques in many diverse areas of chemistry. Of particular significance is the account of Graphics-Aided NMR which will become an inprotant development in the use of NMR spectroscopy.

  • New Techniques for the Study of Electrodes and Their Reactions

    • 1st Edition
    • Volume 29
    • R.G. Compton
    • English
    Volume 29 gives an account of new techniques for the study of electrodes and their reactions. It extends and complements Volumes 26 and 27 of the series which provide an introductory treatment of modern electrochemical methodology and reactions. This volume covers the various branches of spectroelectrochemis... and also some recent purely electrochemical advances. In-situ spectroelectrochemic... techniques are covered by chapters on infrared, Raman, EPR, ellipsometry, electroreflectance, and photocurrent spectroscopy. Ex-situ UHV experiments are treated in a separate chapter. New electrochemical directions are described in chapters on hydrodynamic methods, channel electrodes, and microelectrodes. A final chapter covers computing strategies for the on-line accumulation and processing of electrochemical data.

  • Advances in Heterocyclic Chemistry

    • 1st Edition
    • Volume 44
    • English

  • Advances in Chemical Engineering

    • 1st Edition
    • Volume 14
    • English

  • Advances in Carbohydrate Chemistry and Biochemistry

    • 1st Edition
    • Volume 46
    • English

  • The Alkaloids: Chemistry and Pharmacology

    • 1st Edition
    • Volume 34
    • English

  • Analytical Artifacts

    GC, MS, HPLC, TLC and PC
    • 1st Edition
    • Volume 44
    • B.S. Middleditch
    • English
    This encyclopaedic catalogue of the pitfalls and problems that all analysts encounter in their work is destined to spend more time on the analyst's workbench than on a library shelf. The author has dedicated the book to ``the innumerable scientists who made mistakes, used impure chemicals and solvents, suffered the consequences of unanticipated side-reactions, and were otherwise exposed to mayhem yet were not too embarrassed to publish their findings''. Traditionally, the mass spectroscopist or gas chromatographer learnt his trade by participating in a 4-6 year apprenticeship as graduate student and post-doctoral researcher. Generally, no formal training was provided on the things that go wrong, but this information was accumulated by sharing in the experiences of colleagues. Nowadays, many novice scientists simply purchase a computerized instrument, plug it in, and use it. Much time can be wasted in studying and resolving problems due to artifacts and there is also a strong possibility that artifacts will not be recognized as such. For example, most analysts realize that they should use glass rather than plastic containers; but few of them would anticipate the possibility of plasticizer residues on glassware washed using detergent from a plastic bottle.This book is an easy-to-use compendium of problems encountered when using various commonly used analytical techniques. Emphasis is on impurities, by-products, contaminants and other artifacts. A separate entry is provided for each artifact. For specific chemicals, this entry provides the common name, mass spectrum, gas chromatographic data, CAS name and registry number, synonyms and a narrative discussion. More than 1100 entries are included. Mass spectral data are indexed in a 6-peak index (molecular ion, base peak, second peak, third peak) and there are also formula, author and subject indexes. An extensive bibliography contains complete literature citations.The book is designed to be used. It will not only allow experienced analysts to profit from the mistakes of others, but it will also be invaluable to other scientists who use analytical instruments in their work.

  • Strategies and Tactics in Organic Synthesis

    • 1st Edition
    • Volume 2
    • Thomas Lindberg
    • English
    This is a unique account of the synthesis of organic molecules. All of the contributors are acknowledged experts in organic synthesis.

  • Natural Products Isolation

    Separation Methods for Antimicrobials, Antivirals and Enzyme Inhibitors
    • 1st Edition
    • Volume 43
    • G.H. Wagman + 1 more
    • English
    This new book encompasses, in great detail, the most recent progress made in the isolation and separation of natural products. It covers antibiotics, marine and plant-derived substances, enzyme inhibitors and interferons. The most recent separation methodology is described. Although there is a bias toward antibiotics, it was done because this is still the largest natural products area of research.The fourteen chapters are written by experts in their respective fields. The first two chapters are largely devoted to new methodology applied to purification of a variety of compounds. They include an extensive review and new applications of counter-current chromatography and the newly emerging HPLC-photodiode array technology. Chapter 3 provides a review of affinity chromatography applied to the separation of antibiotics for the first time. Next are chapters on antimicrobials with an update on all the most recent &bgr;-lactam (after 1976) discoveries. A comprehensive review of a very important class of antiparasitic agents - the avermectins - follows. An update of isolation and purification of a variety of marine-derived compounds is next. The succeeding chapter is a comprehensive review of the most recent developments in isolation and purification of interferons. This is followed by a discussion of enzyme inhibitors and their isolation and purification and ties in with a chapter on plant-derived natural products, some of which are also in this same category. The final chapter is a futuristic essay indicating the isolation of minute amounts of natural products and the fascinating biological properties which they possess.The book has extensive isolation schemes, tables, figures and chemical structures. In many instances a short summary of the producing organism, brief chemical description and structure and biological activity of the compounds is presented. Detailed information of extraction, separation and purification techniques follow. Each chapter has an extensive bibliography and, where applicable, an appendix showing sources of materials and equipment. A detailed index to the subject matter is included at the end of the book.The book thus offers the reader: up-to-date reviews (including 1988) of specific topics in the natural products field not to be found elsewhere; information on new chromatographic methods and techniques described in sufficient detail to be utilized by investigators in this area of research; and extensive references to enable the serious researcher to pursue particular information. It will appeal to pharmaceutical and natural products researchers and is a valuable acquisition for university chemistry and biochemistry departments.

  • Vitamins and Hormones

    • 1st Edition
    • Volume 44
    • English

  • Separation of Individual Compound Classes

    • 1st Edition
    • Volume B
    • English

  • Profiles of Drug Substances, Excipients and Related Methodology

    • 1st Edition
    • Volume 17
    • English

  • Cumulative Subject Index, Volumes 102-119, 121-134

    • 1st Edition
    • Volume 140
    • English
    The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

  • Nuclear Magnetic Resonance Spectroscopy

    • 2nd Edition
    • Frank A. Bovey + 2 more
    • English
    Nuclear Magnetic Resonance Spectroscopy, Second Edition focuses on two-dimensional nuclear magnetic resonance (NMR) spectroscopy, high resolution NMR of solids, water suppression, multiple quantum spectroscopy, and NMR imaging. The selection first takes a look at the fundamental principles and experimental methods. Discussions focus on the NMR phenomenon, dipolar broadening and spin-spin relaxation, nuclear electric quadrupole relaxation, saturation, magnetic shielding and chemical shift, magnetic field, transitions between the nuclear energy levels, and resolution and sensitivity considerations. The manuscript then ponders on chemical shift, coupling of nuclear spins, and nuclear relaxation and chemical rate processes. Topics include spin lattice relaxation, spin-spin relaxation, spin decoupling and associated techniques, and description and analysis of spin systems. The text examines two-dimensional NMR spectroscopy, macromolecules, and NMR of solids, including magic angle spinning, cross polarization, proton dipolar broadening, biopolymers, and chain motion in macromolecules. The selection is a valuable source of data for readers interested in nuclear magnetic resonance spectroscopy.

  • Advances in Heterocyclic Chemistry

    • 1st Edition
    • Volume 43
    • English

  • The Alkaloids: Chemistry and Pharmacology

    • 1st Edition
    • Volume 32
    • English

  • The Alkaloids: Chemistry and Pharmacology

    • 1st Edition
    • Volume 33
    • English

  • Advances in Quantum Chemistry

    • 1st Edition
    • Volume 19
    • English

  • Automatic Methods of Analysis

    • 1st Edition
    • Volume 9
    • M. Valcárcel + 1 more
    • English
    This new monograph provides a comprehensive overview of the state of the art of the automation of laboratory processes in analytical chemistry. The topics have been chosen according to such criteria as the degree of consolidation, scope of application and most promising trends.The first part of the book begins with the basic principles behind the automation of laboratory processes, then describes automatic systems for sampling and sample treatment. In the second part the principal types of analysers are discussed: continuous, batch and robotic. The third part is devoted to the automation of analytical instrumentation: spectroscopic, electroanalytical and chromatographic techniques and titrators. The last part presents some examples of the application of automation to clinical chemistry, environmental pollution monitoring and industrial process control.The text is supplemented by 290 figures and 800 literature references. It is written primarily for scientists directly involved in laboratory work and those responsible for industrial planning and control, research centres, etc. It will also be of interest to analytical chemists wishing to update their knowledge in this area, and will be of especial interest to scientists directly related to environmental sciences or clinical chemistry.

  • Advances in Inorganic Chemistry

    • 1st Edition
    • Volume 32
    • English

  • Quantitative Gas Chromatography for Laboratory Analyses and On-Line Process Control

    • 1st Edition
    • Volume 42
    • G. Guiochon + 1 more
    • English
    Here is an invaluable new book on quantitative gas chromatography which explains how the method can - or should - be used for accurate and precise analysis. Gas chromatography is firmly established as one of the few major methods for the quantitative analysis of complex mixtures. It is fast, accurate and inexpensive, with a broad range of applications. It has however become very complex and involved: over 200 stationary phases, more than 10 detector principles and several very different column types are available from among the catalogs of over 100 manufacturers and major retailers. The progressive changes in the nature of gas chromatography have created new needs for information which are not satisfied by the literature presently available.This book provides a complete discussion of all the problems involved in the achievement of quantitative analysis by gas chromatography, whether in the research laboratory, in the routine analysis laboratory or in process control. For this reason the presentation of theoretical concepts has been limited to the essential, while extensive explanations have been devoted to the various steps involved in the derivation of precise and accurate data. This starts with the selection of the instrumentation and column, continues with the choice of optimum experimental conditions, then calibration and ends with the use of correct procedures for data acquisition and calculations. Finally, there is almost always a way to reduce errors and an entire chapter deals with this single issue. Numerous relevant examples are presented.The first part of the book presents the theoretical background, simple enough to be understood by all analytical chemists, but still complete and up-to-date. It discusses the problems of flow dynamics, retention and band broadening. The changes in band profile associated with column overloading are explained without much recourse to mathematics. The second part describes the gas chromatograph and discusses the properties of each of its parts: gas flow and pressure controller sampling system, oven, column switching valves, detectors. The different implementations, their advantages and drawbacks are discussed and compared. In addition, three chapters present packed column technology, open tubular column technology and some sophisticated new phase systems, respectively. The new phase systems described use adsorbents, modified by coating or grafting organic phase, and carrier gases containing vapors which are sorbed by the stationary phase and modify it, such as steam. The third part discusses the applications in qualitative and quantitative analysis. Calibration, peak integration, sources of errors arising from the various parts of the instrument as well as from the measurement process itself are carefully described in four detailed chapters. Methods to carry out accurate and precise analysis are presented. A last chapter is devoted to process control analysis and gives a number of detailed examples of applications. A lexicon explaining the most important chromatographic terms and a detailed index complete the book.This is a book which no chemical analyst should be without. It should be on the library shelf of all universities, instrument companies and any laboratory and plant where gas chromatography is used.

  • The Biochemistry of Plants

    Carbohydrates
    • 1st Edition
    • Volume 14
    • Walter Stumpf + 1 more
    • English
    The Biochemistry of Plants, Volume 14: Carbohydrates provides information pertinent to the fundamental aspects of plant biochemistry. This book deals with the function and structure of the plant cell wall by describing the physical and chemical properties of cell wall components. Organized into 11 chapters, this volume begins with an overview of hexose phosphate metabolism in nonphotosynthetic tissues. This text then examines the findings in fructan structures, conformations, and linkages, the enzymes involved in fructan synthesis and degradation, and their cellular regulation, location, and metabolic role in plants. Other chapters consider the methods employing enzymes to determine starch structure. This book discusses as well the different biosynthetic modes of plant cell walls. The final chapter deals with the various environmental factors that influence expression of the ?-amylase gene, suggesting how molecular biology may help in understanding carbohydrate biochemistry and the enzymes involved in carbohydrate synthesis and metabolism. This book is a valuable resource for plant biochemists.

  • Aqueous Size-Exclusion Chromatography

    • 1st Edition
    • Volume 40
    • P.L. Dubin
    • English
    The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicproce... developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole.This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.

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