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Books in Life sciences

  • Cell Culture and Its Application

    • 1st Edition
    • Ronald Acton
    • English
    Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research. The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology. The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-vir... interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control. Cell biologists and researchers will find this book invaluable.

  • The Importance of laboratory animal genetics Health, and the Environment in Biomedical Research

    • 1st Edition
    • Edward C. Jr. Melby
    • English
    The Importance of Laboratory Animal Genetics, Health, and the Environment in Biomedical Research documents the proceedings of the Fifth Charles River International Symposium on Laboratory Animals, in Heidelberg, Federal Republic of Germany, March 14-16, 1983. These papers examine how the health and genetic monitoring of laboratory animals, coupled with environmental influences, affect the investigations of oncologists, toxicologists, or pharmacologists. The book is organized into four parts. Part I focuses on the health monitoring of laboratory animals for biomedical research. It includes studies on the effects of health and health monitoring in toxicology studies, oncology studies, and pharmacologic studies. Part II deals with the genetic monitoring of laboratory animals. It examines the causes of genetic alternation in laboratory animals and ways to prevent them. Part III considers the environmental monitoring that is necessary for research on laboratory animals. Part IV on new research frontiers includes studies on the production of monoclonal antibodies for the experimental and therapeutic modulation of laboratory animals, and the quality control aspects of animal experimentation.

  • T and B Lymphocytes: Recognition and Function

    • 1st Edition
    • Fritz Bach
    • English
    T and B Lymphocytes: Recognition and Function is the 16th volume of the 1979 ICN-UCLA Symposia on Molecular and Cellular Biology. This book is organized into seven sections, encompassing 74 chapters that summarize molecular level progress in the field of immunobiology. It emphasizes cell membrane, techniques developed for its analysis, and varied products of the major histocompatibility complex, as well as other receptors on cells of the immune system. The book starts by describing two approaches for molecular association evaluation. First is the use of artificial membranes or lipid vesicles for the incorporation of antigens for recognition by T-lymphocytes both at the afferent and efferent levels. Second is the chemical crosslinking of cell surface components with a variety of different crosslinking reagents. This is followed by a discussion on the molecular nature of a T-cell receptor that provides insights of the target(s) recognized by T-lymphocytes. Several chapters discuss data dealing with the issue of the apparent differential recognition by T- and B-cells while both use the same VH genes. Antiidiotypic sera, together with the activity of ""regulator"" T-cells, are also discussed, with regard to their role in networks of regulation of the immune response. This book presents works demonstrating that human T-lymphocytes can also be cloned and both noncytotoxic proliferating T-lymphocytes and Tc can be so obtained. This is along with the continuing studies regarding heterogenity of B-lymphocytes as well as macrophages. Part V focuses on the interaction between lymphocyte and virus and molecular modifications of viral-infected cells. The subsequent section deals with recognition, reactivities, and function of T- and B-lymphocytes. Considerable chapters in this section discuss T-cell cytotoxicity, dichotomy of MHC control over anti H-Y cytotoxic T-cell responses, mouse alloantibodies, and mixed lymphocyte reactions. The concluding part describes immunological tools, such as synthetic membranes and cloned T-cells with biological function.

  • Microbiological Quality of Foods

    • 1st Edition
    • L Slanetz
    • English
    Microbiological Quality of Foods contains the proceedings of a conference held in Franconia, New Hampshire, on August 27-29, 1962. Contributors review the state of knowledge of foodborne diseases and discuss the use and efficiency of microbiological tests and standards for food quality from the academic, regulatory, and industrial standpoints. Problems related to the use of microorganisms as an index of food quality are given special attention. This book includes a consideration of total counts, coliforms, fecal streptococci, and the detection of specific pathogens. This text is organized into 26 chapters and begins with an overview of the status of microbiological tests and standards that have been developed to ensure food quality. The book then discusses the concerns of regulators at the federal and local levels concerning food microbiology, particularly the safety or wholesomeness of foods. The next chapters focus on industry perspectives regarding food safety; the role of universities in food microbiological research; and problems and challenges presented by foodborne diseases. The book also introduces the reader to staphylococcal enterotoxins, halophilic bacteria, botulism, and Clostridium perfringens that causes food poisoning. This book is a valuable resource for those involved in food microbiology, science and technology, and industry; bacteriology; and public health.

  • Laboratory Safety Theory and Practice

    • 1st Edition
    • Anthony Fuscaldo
    • English
    Laboratory Safety: Theory and Practice focuses on theoretical aspects of the hazards the students, technicians, and scientists encounter in the laboratory. It presents methods of risk assessment that can be applied to technologies as they are translated from the scientist’s mind to the laboratory bench. It is organized into three sections designated as General Laboratory Safety, Biological Laboratory Safety, and Medical and Psychological Factors. The first section, encompassing three chapters, discusses hazards found in almost all laboratories; pertinent safety theories and practices; ubiquitous compounds that are either toxic or carcinogenic and guidelines for their use; and radiation hazards. Chapters 4 to 7 focus on the safety in the biological laboratory. Discussions on relatively complex group of viruses, approach to recombinant DNA research, and awareness on the possible hazards associated with the field are included in this book. Chapters 6 and 7 present design and function of biohazard laboratories and the hazards relating to laboratory animals. The final section discusses medical surveillance of persons at risk and the psychological factors involved in accident control. It presents a comprehensive list of chemical agents, their sources, subsequent physical effects, and the accepted mode of medical surveillance. Various genetic screening tests and their potential use for the evaluation of presumptive and actual mutagens are also covered. This book is ideal for safety and design engineers, students, technicians, and scientists.

  • Plant Cold Hardiness and Freezing Stress

    Mechanisms and Crop Implications
    • 1st Edition
    • P.H. Li
    • English
    Plant Cold Hardiness and Freezing Stress: Mechanisms and Crop Implications contains the proceedings of an International Plant Cold Hardiness Seminar, held in St. Paul, Minnesota on November 2-4, 1977. Organized into seven parts, this book contains a collection of valuable articles on the advances in plant cold hardiness research. This text first addresses the freezing stress in plants in nature, in the field, or as a result of laboratory experiments intended to explain the process. Some chapters follow that discuss the effect of cold acclimation and freezing on plant's cell membrane, the mechanism of cold acclimation in plants, and the super cooling stress in plants. The survival, breeding, cryopreservation, and cryoprotection of plants are also explained.

  • The Structure and Function of Nervous Tissue V5

    Structure III and Physiology III
    • 1st Edition
    • Geoffrey Bourne
    • English
    The Structure and Function of Nervous Tissue, Volume V: Structure III and Physiology III covers topics relating to nerve growth factor (NGF), neuroglia, central myelinated axon, adrenal medulla, and saccus vasculosus of the nervous system. The book begins with the physicochemical properties of the NGF molecule, followed by the neuroglial participation in the removal of debris from damaged central nervous system (CNS). It discusses the regional distribution and biochemical characteristics of two steroid-binding systems, one for estradiol and the other for corticosterone. It also presents studies on “split-brain”, an expression coined by Sperry (1961) to characterize an organism whose brain has been partially divided into two by surgical transection of the commissural fibers crossing the midline, in lower vertebrates, mammals, and primates. The book ends with electrophysiological studies of learning in simplified nervous system preparations. This book serves as a reference work for graduate students in a variety of disciplines and for those specializing in particular aspects of nervous tissue study.

  • Insect Juvenile Hormones

    Chemistry And Action
    • 1st Edition
    • Julius Menn
    • English
    Insect Juvenile Hormones: Chemistry and Action reviews the state of knowledge on juvenile hormones (JH) and analogs. The book emerged from the presentations and deliberations at the Symposium on the Chemistry and Action of Insect Juvenile Hormones, sponsored by the Division of Pesticide Chemistry of the American Chemical Society at the 162nd National Meeting of the Society in Washington, D. C., September 12-17, 1971. The book summarizes what is known about JH their analogs; their chemistry; their biological effects and mode of action; their biochemical fate in target and nontarget organisms; and their stability. Although divided into three general areas for convenience—biologic... biochemical, and chemical—the text in its entirety is a multidisciplinary discourse, a foundation upon which the understanding of the action or inaction of JH and JH-active chemicals may be based, which hopefully will provide a springboard for future direction and research. This book will be of interest to chemists, biochemists, biologists, endocrinologists, entomologists, insect physiologists, and others concerned with insect development and control.

  • From Gene to Protein: Translation into Biotechnology

    • 1st Edition
    • Fazal Ahmad
    • English
    From Gene to Protein: Translation into Biotechnology is the 15th volume in the continuing series under the title ""Miami Winter Symposia"". The theme of the symposium is the translation of the basic research findings into the practical application of biotechnology. This book summarizes methodology and its applications that lie behind the practical innovations. The book starts with reviews of techniques of eukaryotic cell culture, hybridoma technology and uses, and the in vitro synthesis of DNA and its use in the generation of protein analogs. Considerable space is devoted to development of monoclonal antibodies that promises to be the dominating tool of medical technology, both for diagnosis and therapy. Cloning into eukaryotic cells and methods of increasing the levels of gene expression are included. These topics reflect areas of intensive research that have important commercial and clinical value. Core chapters describe biological activities of cloned gene products, including reports on trials with human subjects of interferon, human insulin, and growth hormone. A panel session on horizons in biotechnology is also provided, looking forward to the directions of future research and its applications. Biotechnologists, cell biologists, scientists, researchers, teachers, and students will greatly benefit from this book.

  • Research Films in Biology, Anthropology, Psychology, and Medicine

    • 1st Edition
    • Anthony Michaelis
    • English
    Research Films in Biology, Anthropology, Psychology, and Medicine is an encyclopedic account of the many uses of research films in the fields of biology, anthropology, psychology, and medicine. The book looks at cinemicrography, the making of human record films, and quantitative methods inherent in all scientific cinematography such as medical and X-ray cinematography. This volume is organized into three sections encompassing 10 chapters and begins by considering the definition of research film and scientific cinematography, touching on topics such as the advantages and limitations of scientific cinematography and methods used to preserve and store the research film. The next chapters discuss the fundamental principles of cinemicrography as a research tool; the value of cinematography in biological investigations, including the study of animal behavior; and the theoretical and practical considerations in the use of cinematography in the human sciences, such as anthropology, psychology, and psychiatry. The book also methodically introduces the reader to medical applications of cinematography and the techniques of X-ray cinematography, and then concludes with relevant examples of the use of cinematography in medical research and diagnosis. This book is a valuable resource for scientists and cinematographers.

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