Skip to main content

Books in Life sciences

Experimental Manipulation of Gene Expression
1st Edition
June 28, 2014
Masayori Inouye
English
Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
In Vitro Methods in Cell-Mediated Immunity
1st Edition
June 28, 2014
Barry R. Bloom + 1 more
English
In Vitro Methods in Cell-Mediated Immunity focuses on methods for approaching cell-mediated immune responses in vitro. This book provides, in simplified in vitro systems, a basis for understanding the mechanism of the in vivo response and discusses useful and reliable in vitro tests for cell-mediated immune responses in humans, where in vivo testing is often not possible. The topics discussed include factors and activities produced in vitro by lymphocytes; biological implications of in vitro phenomena; and requirements and prospects for improved methodology. The leucocyte migration technique for in vitro detection of cellular hypersensitivity in man; proliferation of human blood lymphocytes stimulated by antigen in vitro; and virus plaque assay for antigen-sensitive are also elaborated in this text. This publication is a good reference for microbiologist and immunologists, including medical students researching on in vitro models for cell-mediated immune reactions.
Horizons of Bioenergetics
Proceedings of a Symposium held at Bloomington, Indiana October 12-15, 1970
1st Edition
June 28, 2014
Anthony San Pietro + 1 more
English
Horizons of Bioenergetics provides information pertinent to the fundamental aspects of bioenergetics. This book covers a variety of topics, including oxidative phosphorylation, energy conservation, ATP formation, metabolic regulation, glycolysis, and photosynthesis efficiency. Organized into five sessions encompassing 23 chapters, this book begins with an overview of the major landmarks in biochemical evolution that are most relevant to the discussion on bioenergetics. This text then reviews the knowledge about ATP generation by substrate level phosphorylation by anaerobic bacteria that ferment organic compounds. Other chapters consider the resolution and reconstitution of oxidative phosphorylation. This book discusses as well another energy-requiring activity associated with electron transport in mitochondria that may take primacy over oxidative phosphorylation. The final chapter deals with the possibility that conformational change can induce major shifts in electro-chemical potential and provide a mechanism for energy storage and conversion. This book is a valuable resource for biochemists, scientists, and research workers.
Clinical Biochemistry of Domestic Animals
3rd Edition
June 28, 2014
Jiro J. Kaneko
English
Clinical Biochemistry of Domestic Animals, Third Edition, represents a major revision of the previous editions. Since the publication of the first edition of ""Clinical Biochemistry of Domestic Animals,"" veterinary clinical biochemistry has enjoyed a virtual explosion of new knowledge commensurate with the increased importance of companion animals, the livestock industry, and experimental animals. This third edition brings together some of the most important areas of clinical biochemistry pertinent to these sectors. For this purpose, new chapters on the reproductive hormones and clinical enzymology have been added, in addition to a rewriting of the chapters on renal function and plasma proteins and extensive revisions of all other chapters. The volume contains 18 chapters and opens with discussions of carbohydrate and lipid metabolism and associated disorders. This is followed by separate chapters on serum proteins and the dysproteinemias; porpyhrins; clinical enzymology; liver, pancreatic, and kidney function; and the physiology and pathophysiology of body fluids. Subsequent chapters deal with pituitary, adrenal, and thyroid function; skeletal muscle function; calcium, phosphorus, magnesium, and iron metabolism; the mechanisms of homeostasis; and cerebrospinal fluid physiology.
Advances in Lipid Research
1st Edition
Volume 11
June 28, 2014
Rodolfo Paoletti + 1 more
English
Advances in Lipid Research, Volume 11 is a seven-chapter text that discusses a wide range of topics in the field, including steroid metabolism, phytosterols, and fatty acid transport. The first chapter reviews the metabolic role of lecithin: cholesterol acyltransferase. The next chapter deals with the simple means of separation and quantitation of serum lipoproteins. This chapter describes the variations of the reaction involving lipoproteins, divalent cations, and polyanionic substances, both organic and inorganic. Other chapters discuss the fatty acid metabolism by brain tissue, the concept of the blood-brain barrier and its role in fatty acid uptake, as well as the many alterations in bile acid structure due to their interaction with the intestinal microflora. A chapter focuses on the multiple aspects of plant sterol chemistry, function, and metabolism. The last chapters examine the mechanism of insect utilization and metabolism of dietary sterol. These chapters also cover the role of lipids in viral structure and metabolism. This book will prove useful to lipid chemistry, biochemists, and researchers.
Advances in Lipid Research
1st Edition
Volume 19
June 28, 2014
Rodolfo Paoletti + 1 more
English
Advances in Lipid Research, Volume 19 is a seven-chapter text that covers the physical and biological aspects of lipid metabolism. The first chapter deals with the interactions between circulating lipids and lipid proteins and the arterial tissue matrix. The subsequent chapter discusses the apolipoprotein C metabolism, the roles of lecithin-cholesterol acyltransferase and cholesterol ester transfer protein in intravascular cholesterol transport. Other chapters explore the significance of bile acid biosynthesis for cholesterol homeostasis, the metabolism of phosphatidylserine, and gas chromatographic and mass spectrometry analysis of prostanoids. The last chapter considers the role of dietary fiber in health and disease. This book is of great value to lipid and enzyme chemists, biochemists, and researchers.
Advances in Lipid Research
1st Edition
Volume 7
June 28, 2014
Rodolfo Paoletti + 1 more
English
Advances in Lipid Research reviews advances in lipid research and covers topics ranging from lipid histochemistry and control of plasma and liver triglyceride kinetics by carbohydrate metabolism and insulin. Lipid metabolism in tissue culture cells is also discussed, along with the carcinogenicity of steroids and the chemical and biological properties of heated and oxidized fats. Comprised of five chapters, this volume begins with an introduction to the histochemistry of lipids in both its qualitative and quantitative aspects, followed by an analysis of the roles of carbohydrate metabolism and insulin in relation to their control of the metabolism of plasma and liver triglycerides. Lipid metabolism in many tissue culture systems is the subject of the next chapter. The experimental and theoretical aspects of the connection between cancer and steroids are also examined. The last chapter deals with the possible harmful effects of heating dietary fats and how heating may alter the chemical and biological properties of fats. Attention is paid to auto-oxidation, carcinogenicity, and the interaction of heated fats with other dietary components. This monograph will be useful to students, practicing professionals, and research workers in biology and biochemistry.
Molecular Approaches to Neurobiology
1st Edition
June 28, 2014
Ian R. Brown
English
Molecular Approaches to Neurobiology deals with molecular approaches to the analysis of the nervous system. This book surveys the current state of knowledge in a number of areas of molecular neurobiology that includes the subcellular level of chromatin structure, complexity of RNA synthesis, role of hormones in cell differentiation, and molecular correlates between neuropeptides and behavior. A comprehensive review of procedures for the isolation of specific brain cells and their experimental use is also provided at the end of this text. This publication is useful to those working in the field of neurochemistry and those engaged in morphological and physiological approaches to the analysis of the nervous system, as well as molecular biologists and biochemists working with non-neural tissues.
Current Topics in Bioenergetics
Volume 5
1st Edition
June 28, 2014
D. Rao Sanadi + 1 more
English
Current Topics in Bioenergetics, Volume 5 provides information pertinent to the molecular properties of purified enzymes and defined reactions. This book presents the development in the research on oxidative phosphorylation. Organized into nine chapters, this volume begins with an overview of the contributions to the knowledge of membrane structure based on X-ray diffraction analysis. This text then examines the reactions of chlorophyll in model systems and the luminescence linked with light absorptions, which relate to the early events in photosynthesis. Other chapters relate spectroscopic and EPR measurements to redox changes linked with energy coupling in the mitochondrial electron carriers. This book discusses as well the role of soluble proteins in the energy transfer process of oxidative phosphorylation. The final chapter deals with the chemical and structural properties of the photoreceptors in the visual process. This book is a valuable resource for biophysicists, physiologists, biologists, biochemists, physical chemists, and research workers.
Clinical Immunobiology
1st Edition
Volume 3
June 28, 2014
Fritz H. Bach + 1 more
English
Clinical Immunobiology, Volume 3 covers a variety of methods used for assaying the immune status of an individual. This volume is composed of 21 chapters and begins with a presentation of the background and issues to which clinical tests would be applied. The succeeding chapters outline the methodological procedures used in the analyses. Other chapters present some examples of the kinds of data that can be generated with the procedures used and provide guidelines for interpretation of the tests. The remaining chapters discuss the value of the immunological test procedures in differential diagnosis and analysis of diseases. These chapters also explore the usefulness of these procedures in prognosis of disease and the consequences of immunological manipulation undertaken for treatment or prevention of disease. This book will be of great value to clinical immunobiologists, biochemists, and researchers.

Related subjects