Books in Laboratory instrumentation and automation

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Analytical Ultracentrifugation

• 1st Edition
• Volume 562
• September 24, 2015
• James L Cole
• English
• Hardback 978-0-12-802908-4

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• eBook 978-0-12-802909-1

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Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.

Determination of Beta-Blockers in Biological Material

• 1st Edition
• Volume 4
• October 22, 2013
• V. Marko
• English
• eBook 978-1-4832-9069-0

  9 7 8 - 1 - 4 8 3 2 - 9 0 6 9 - 0

This is the third volume of a sub-series entitled Evaluation of Analytical Methods in Biological Systems. (The first two were Analysis of Biogenic Amines edited by G.B. Baker and R.T. Coutts, and Hazardous Metals in Human Toxicology edited by A. Vercruysse).This latest volume addresses beta-blockers - an area of research for which a Nobel Prize in Medicine was awarded in 1988. It provides an up-to-date and comprehensive coverage of the theory and practice of the determination of beta-blockers in biological material. Two main fields of research are dealt with in this book: analytical chemistry and pharmacology, and, as it deals with drugs used in clinical practice, it is also related to a third area: therapy. Thus, it offers relevant information to workers in all three fields.Some 50 beta-blockers are discussed, and nine methods of analysis. The methods are divided into three groups: optical, chromatographic, and saturation methods. In addition to the analytical methods themselves, sample handling problems are also discussed in detail, as is the information content of the analytical results obtained. Special chapters are directed towards those working in pharmacology and pharmacokinetics. Finally, as recent evidence points to the increased importance of distinguishing optical isomers of drugs, a chapter on the determination of optical isomers of beta-blockers in biological material is also included. An extensive subject index and two supplements giving retention indices and structures of beta-blockers complete the book.This is the first book to treat beta-blockers from the point of view of their determination and to discuss in detail the use of analytical methods for beta-blockers. It will thus appeal to a wide-ranging readership.

Autoradiography and Immunocytochemistry

• 1st Edition
• October 22, 2013
• M.A. Williams
• Audrey. M. Glauert
• English
• eBook 978-1-4832-9387-5

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Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining” with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.

High Performance Liquid Chromatography

• 1st Edition
• October 22, 2013
• Mary T. Gilbert
• English
• eBook 978-1-4832-8129-2

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High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Essays on Analytical Chemistry

• 1st Edition
• October 2, 2013
• Erkki Wänninen
• English
• eBook 978-1-4831-8814-0

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Essays on Analytical Chemistry: In Memory of Professor Anders Ringbom is a collection of analytical chemistry papers and research studies in honor of the memory of Professor Anders Ringbom, a highly esteemed researcher and teacher. The papers are grouped under the following headings: Chemical Equilibria, Titrations, Photometric Analysis, Electrochemistry, Separations, Trace Analysis, Kinetic Analysis, and Other Analytical Topics. This book is organized into eight parts encompassing 52 chapters. The first part deals with the concept of chemical equilibria in acid-base and metal complexes. The next parts cover the applications of different titration techniques, photometric analysis, electrochemistry, and separation techniques. Other parts highlight the principles and application of trace analysis, including the determination of heavy metals and airborne particulates. The last parts contain papers that examine the analytical application of the rate phenomena of several chemical reactions. These parts also tackle the topics of sampling, statistical analysis in analytical chemistry, and the features of photoelectron spectroscopy and capillary electrophoresis. This book will be of great value to analytical chemists, researchers, and analytical chemistry students.

TRAC: Trends in Analytical Chemistry

• 1st Edition
• September 24, 2013
• U A Th Brinkman + 2 more
• English
• eBook 978-1-4832-7456-0

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TRAC: Trends in Analytical Chemistry, Volume 7 provides information pertinent to the trends in the field of analytical chemistry. This book discusses a variety of topics related to analytical chemistry, including biomolecular mass spectroscopy, affinity chromatography, electrochemical detection, nucleosides, and protein sequencing. Organized into 63 parts encompassing 158 chapters, this volume begins with an overview of the significance of quality and productivity in the analytical laboratory. This text then presents a comprehensive review on alcohol dehydrogenases, immobilization, and applications in analysis and synthesis. Other chapters consider the various tests for determining the excellence of quantitative assays available for analysts to utilize for method validation. This book discusses as well the primary challenge of neuropharmacologists to relate physiological functions to the many ligand binding sites identified in brain tissue. The final chapter deals with the fundamentals and applications of biosensors. This book is a valuable resource for analytical chemists, chemical engineers, clinical chemists, neuropharmacologists, and scientists.

TRAC: Trends in Analytical Chemistry

• 1st Edition
• September 17, 2013
• U A Th Brinkman + 2 more
• English
• eBook 978-1-4832-7470-6

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TRAC: Trends in Analytical Chemistry, Volume 10 presents relevant topics in global analytical chemistry research. This book discusses the potential of flow injection analysis for water quality monitoring. Organized into 27 parts encompassing 67 chapters, this book begins with an overview of the amount of published information on analytical chemistry research. This text then examines the analytical technique in the electrophoretic separations in narrow bore tubes, which is capable of rapid, high-resolution separations of water-soluble components in small sample volumes. Other chapters consider the application of polynomial and B-spline interpolation to the description of cyclic voltammetric features. This book discusses as well the methods used to investigate the properties of ceramic high-transition-temperature superconductors. The final chapter deals with the importance of monitoring and protecting the environment based on measurement campaigns. This book is a valuable resource for analytical chemists, environmental chemists, and biochemists. Pharmacologists, scientists, students, researcher workers, and other practitioners will also find this book useful.

Comprehensive Sampling and Sample Preparation

• 1st Edition
• December 31, 2012
• Josep M. Bayona + 6 more
• English
• eBook 978-0-12-381374-9

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Comprehensive Sampling and Sample Preparation is a complete treatment of the theory and methodology of sampling in all physical phases and the theory of sample preparation for all major extraction techniques. It is the perfect starting point for researchers and students to design and implement their experiments and support those experiments with quality-reviewed background information. In its four volumes, fundamentals of sampling and sample preparation are reinforced through broad and detailed sections dealing with Biological and Medical, Environmental and Forensic, and Food and Beverage applications. The contributions are organized to reflect the way in which analytical chemists approach a problem. It is intended for a broad audience of analytical chemists, both educators and practitioners of the art and can assist in the preparation of courses as well in the selection of sampling and sample preparation techniques to address the challenges at hand. Above all, it is designed to be helpful in learning more about these topics, as well as to encourage an interest in sampling and sample preparation by outlining the present practice of the technology and by indicating research opportunities.

A Practical Guide to HPLC Detection

• 1st Edition
• December 2, 2012
• Donald Parriott
• English
• eBook 978-0-323-14061-4

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This guide for the practicing chromatographer who wants a ready source of information on HPLC detection explores and compares existing detection systems and detectors, outlines the common problems associated with a given detector, and offers proven approaches to avoiding such problems.

Automatic Titrators

• 1st Edition
• November 14, 2012
• J Philips
• English
• eBook 978-0-323-15956-2

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Automatic Titrators focuses on the contributions and effects of modern automation on volumetric analysis. The book presents titration as a modern instrumental method in this kind of analysis. Divided into nine chapters, the book proceeds by defining the value of automatic titration methods. The text also outlines the general considerations of titrate design wherein instrumental indicators, recorders, and controllers are given emphasis. Automatic potentiometric titrates are also discussed. A historical tracing of these titrators is presented as well as the trends and kinds of modern automatic titrators. The book also touches on automatic photometric and automatic coulometric titrators. Supporting discussions focus on photosensitive devices; photometric titration curves; coulometric circuits; instruments with potentiometric, amperometric, and photometric indication; and multipurpose coulometric titrators. The book ends by fully discussing automatic and continuous titrators, commercially available titrators, and applications of automatic titration methods. The selection can best serve those wanting to explore the function of titrators in volumetric analysis.