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High Performance Liquid Chromatography
- 1st Edition - October 22, 2013
- Author: Mary T. Gilbert
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 5 0 1 9 - 9
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 8 1 2 9 - 2
High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid… Read more
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Request a sales quoteHigh Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.
List of AbbreviationsGlossary of Symbols1 Introduction2 Basic Chromatographic Theory 2.1 Column Capacity Ratio 2.2 Efficiency 2.3 Resolution 2.4 Asymmetry3 Equipment 3.1 Pumps and Gradient Systems 3.2 Injectors 3.3 Connectors 3.4 Detectors 3.5 Data Systems4 The Column 4.1 Column Design 4.2 Packing Materials 4.3 Column Packing and Testing 4.4 Practical Considerations 4.5 Coupling of Columns 4.6 Micro Columns5 Sample Treatment 5.1 Clean-Up Procedures 5.2 Trace Analysis 5.3 Derivatisation 5.4 Post-Column Reaction Detectors6 Separation Methods 6.1 Selection/Optimization of Conditions 6.2 Normal Phase Chromatography 6.3 Liquid-Liquid Partition Chromatography 6.4 Reversed Phase Chromatography 6.5 Ion Exchange Chromatography 6.6 Size Exclusion Chromatography 6.7 Affinity Chromatography 6.8 Inclusion Chromatography 6.9 Chiral Chromatography7 Adjustment of Selectivity by Use of Fluent Additives 7.1 Ion Pair Partition Chromatography 7.2 Chromatography on Dynamically Modified Oxide Gels 7.3 Metal Complexation 7.4 Crown Ethers8 Preparative Liquid Chromatography 8.1 Separation Strategy 8.2 Equipment for Preparative LC 8.3 Materials for Preparative Chromatography 8.4 Applications9 Chiral Chromatography 9.1 Enantiomeric Resolution by Preparation of Diastereoisomer 9.2 Direct Enantiomer Resolution10 Current Trends in the Practice of HPLC 10.1 Columns 10.2 Detectors 10.3 Packing Materials 10.4 Supercritical Fluid Chromatography 10.5 ConclusionsReferencesAppendix I Packing Materials for High Performance Liquid ChromatographyAppendix II Properties of Chromatographic SolventsIndex
- No. of pages: 396
- Language: English
- Edition: 1
- Published: October 22, 2013
- Imprint: Butterworth-Heinemann
- Paperback ISBN: 9781483250199
- eBook ISBN: 9781483281292