Books in Analytical separations

Protein Liquid Chromatography

1st Edition
Volume 61
December 13, 1999
M. Kastner
English
Paperback
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eBook
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Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Advanced Chromatographic and Electromigration Methods in BioSciences

1st Edition
Volume 60
September 28, 1998
I. Mikšík + 3 more
English
Hardback
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eBook
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This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Data Analysis and Signal Processing in Chromatography

1st Edition
Volume 21
May 19, 1998
A. Felinger
English
Hardback
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eBook
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This book gives an overview of the numerical data analysis and signal treatment techniques that are used in chromatography and related separation techniques. Emphasis is given to the description of the symmetrical and asymmetrical chromatographic peak shape models. Both theoretical and empirical models are discussed.The fundamentals of data acquisition, types and effect of baseline noise, and methods of improving the signal-to-noise ratio (either in time or in frequency and wavelet domain) are thoroughly discussed. Resolution enhancement techniques, such as curve fitting, deconvolution by Fourier and wavelet transforms, iterative deconvolution, Kalman filtering and multivariate methods of curve resolution are all discussed with several chromatographic examples. Quantitative analysis by peak area of peak height measurement, the precision and accuracy of the quantitation of stand-alone or overlapping and symmetrical or asymmetrical peaks are treated. In a separate chapter, guidelines are given for the use of transform techniques for the analysis of chromatograms. A statistical description of peak overlap is given in the final chapters. Since the concept of resolution has to be reconsidered when one separates complex mixtures, the problem of resolution and overlap is quantitatively discussed by means of statistical methods, and by using Fourier analysis of the complex chromatogram.Feature... of this book• The ultimate source of numerical techniques to enhance chromatographic data• Gives a detailed description of signal and resolution enhancement techniques in a manner applicable for enhancing not only chromatography, but also spectroscopic and other analytical signals• The first book with a thorough overview of the statistics of peak overlap.This is the first volume to encompass both the simple and more sophisticated methods for the numerical treatment of chromatograms. It is, therefore, the fundamental resource of numerical analysis methods for every analyst.

Gas Chromatography and Mass Spectrometry

1st Edition
August 20, 1996
Fulton G. Kitson + 2 more
English
eBook
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This guide provides, under one cover, a wealth of practical information designed to facilitate the effectiveness of the GC/MS user. Separation conditions for numerous compound types are provided along with derivatized and underivatized compounds. A section on how to interpret mass spectral data, an extensive correlation of ion masses and neutral losses with possible structures, and examples of mass spectra are provided to further aid structure determination. Also included are basic information on instrumentation, ionization methods, quantitation, tips on the operation of mass spectrometers, the best derivatization procedures for a variety of compound types, troubleshooting techniques, and a variety of other information found to be useful to the practicing user of GC/MS instrumentation. This guide would be immediately valuable to the novice as well as the experienced GC/MS user who may not have the breadth of experience covered in this book.

Pharmaceutical and Biomedical Applications of Capillary Electrophoresis

1st Edition
Volume 2
August 13, 1996
S.M. Lunte + 1 more
English
eBook
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The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis.

Data Analysis for Hyphenated Techniques

1st Edition
Volume 17
March 14, 1996
E.J. Karjalainen + 1 more
English
eBook
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Based on a concrete set of working MATLAB programs, the book begins with a short program of snippets describing basic principles and proceeds to a complete application program filling a central analytical need: obtaining pure spectra from the observed overlapping spectra, with standard deviations for thesolutions obtained.The strength of the book is the open nature of the programs. All programs can be read, tested and modified by the user. The mathematical principles needed for the proper treatment of experimental data are thoroughly described. The first part of the book describes how data iscollected, converted and prepared for the actual deconvolution. Practical working algorithms such as the Savitzky-Golay smoothing method are emphasized. The reader can see how searches in spectral libraries can be greatly speeded up by proper formulation of the calculation. Basic signal processing is described by illustrative examples.The main part of the book describes the deconvolution of overlapping chromatographic peaks. Principle component analysis is described and used as a useful tool. The main emphasis is a discussion of a deconvolution method, OSCAR (Optimization by Stepwise Constraining of Alternating Regression), developed by the authors. The results can be validated as OSCAR calculates confidence intervals for the spectra and elution curves.For users who do not want to enter the programs by hand a separate CD-ROM is available. It contains the programs and extensive sample data files. The CD-ROM has instructional multimedia showing step by step how the programs are used for the problems in the book. The MATLAB programs and datafiles can be directly run on Windows® and Macintosh® computers having a MATLAB interpreter.

Advances in Food and Nutrition Research

1st Edition
Volume 38
October 20, 1995
Steve Taylor + 1 more
English
eBook
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The appearance of Volume 38 marks a transition for Advances in Food and Nutrition Research as Steve L. Taylor assumes editorial responsibility for the series. Under John Kinsella's guiding hand, Advances in Food Research strengthened its reputation as the leading publication for comprehensive reviews on important topics in food science, evolving into Advances in Food and Nutrition Research, a title which better reflected his interest in the integral relationships between food science and nutrition. Building on this legacy of quality scholarship, Dr. Taylor brings a fresh perspective to the serial, seeking novel approaches to research in food and nutritional science.

Retention and Selectivity in Liquid Chromatography

1st Edition
Volume 57
January 13, 1995
R.M. Smith
English
eBook
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This book brings together a number of studies which examine the ways in which the retention and selectivity of separations in high-performance liquid chromatography are dependent on the chemical structure of the analytes and the properties of the stationary and mobile phases. Although previous authors have described the optimisation of separations by alteration of the mobile phase, little emphasis has previously been reported of the influence of the structure and properties of the analyte.The initial chapters describe methods based on retention index group increments and log P increments for the prediction of the retention of analytes and the ways in which these factors are influenced by mobile phases and intramolecular interactions. The values of a wide range of group increments in different eluents are tabulated.Different scales of retention indices in liquid chromatography are described for the comparison of separations, the identification of analytes and the comparison of stationary phases. Applications of these methods in the pharmaceutical, toxicology, forensic, metabolism, environmental, food and other fields are reviewed. The effects of different mobile phases on the selectivity of the retention indices are reported. A compilation of sources of reported retention index values are given.Methods for the comparison of stationary phases based on the interactions of different analytes are covered, including lipophilic and polar indices, shape selectivity comparisons, their application to novel stationary phases, and chemometric methods for column comparisons.

Principles and Practice of Modern Chromatographic Methods

1st Edition
November 29, 1994
Kevin Robards + 2 more
English
Hardback
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eBook
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Though many separation processes are available for use in todays analytical laboratory, chromatographic methods are the most widely used. The applications of chromatography have grown explosively in the last four decades, owing to the development of new techniques and to the expanding need of scientists for better methods of separating complex mixtures. With its comprehensive, unified approach, this book will greatly assist the novice in need of a reference to chromatographic techniques, as well as the specialist suddenly faced with the need to switch from one technique to another.

Carbohydrate Analysis

1st Edition
Volume 58
November 11, 1994
Z. El Rassi
English
eBook
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Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE). In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book describes specialized methods for the separation, purification and detection of carbohydrates and glycoconjugates by HPLC and HPCE.Therefore, the objective of the present book is to provide a comprehensive review of carbohydrate analysis by HPLC and HPCE by covering analytical and preparative separation techniques for all classes of carbohydrates including mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharid... (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in food and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc.The book will be of interest to a wide audience, including analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, etc. It will also be a useful reference work for both the experienced analyst and the newcomer as well as for users of HPLC and HPCE, graduates and postdoctoral students.

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