ZIP Metal Ion Transporters
- 1st Edition, Volume 687 - August 31, 2023
- Imprint: Academic Press
- Editor: Jian Hu
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 5 7 7 6 - 2
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 5 7 7 7 - 9
ZIP Metal Ion Transporters, Volume 684 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a va… Read more
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ZIP Metal Ion Transporters, Volume 684 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Biochemistry, Biophysics and Structural Biology, Structure determination of a bacterial ZIP in lipidic cubic phase, Cell-based metal transport assay for animal and plant ZIPs, Measurement of metal binding affinity and transport activity in ZIP transporters using spectroscopic methods, Considerations in production of the (prokaryotic) ZIP family transporters for structural and functional studies, Metal-protein interactions investigated using model systems: thermodynamic, spectroscopic and computational methods, and more.
Additional chapters cover Getting Zinc Into and Out of Cells, In-silico modeling of ZIP9 (TBD), Cellular Function and Regulation, Methods to visualize zinc transporter proteins of the SLC39A family in cells, Assessing metal ion transporting activity of ZIPs: intracellular zinc and iron detection, In vitro studies for studying manganese transport and homeostasis, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology serials
- Updated release includes the latest information on ZIP Metal Ion Transporters
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Unit I. Biochemistry, Biophysics and Structural Biology
1. Structure determination of a bacterial ZIP in lipidic cubic phase
Jian Hu
2. Cell-based metal transport assay for animal and plant ZIPs
Jian Hu
3. Measurement of metal binding affinity and transport activity in ZIP transporters using spectroscopic methods
Bharati Mitra
4. Considerations in production of the (prokaryotic) ZIP family transporters for sturcural and functional studies
Cheng Ma
5. Metal-protein interactions investigated using model systems: thermodynamic, spectroscopic and computational methods
Maurizio Remelli
6. Expression, purification and crystallization of the extracellular domain of a mammalian ZIP4
Tuo Zhang
7. Getting Zinc Into and Out of Cells
Kenneth Merz
8. In-silico modeling of ZIP9 (TBD)
Georgios Scheiner-Bobis
Unit II. Cellular Function and Regulation
9. Methods to visualise zinc transporter proteins of the SLC39A family in cells
Kathryn M. Taylor
10. Assessing metal ion transporting activity of ZIPs: intracellular zinc and iron detection
Zhou Bing
11. In vitro studies for studying manganese transport and homeostasis
Young Ah Seo
12. In vitro studies to evaluate zinc transport ability of ZIPs through analyzing ZNT1 and MT expression responses
Taiho Kambe
13. The optimized method to detect mouse erythrocyte membrane ZIP metal transporters by immunoblotting analysis
Ningning Zhao
14. Dynamic internalization and trafficking of zinc transporter
Chi Zhang
15. Assessing the impact of varying zinc levels in the transcription profiles of human neurons
Robert E. Dempski
16. Quantifying homomeric and heteromeric ZIP complexes using fluorescence correlation and fluorescence cross-correlation spectroscopy
Robert E. Dempski
17. ZIP9-directed androgenic tetra peptides (TBD)
Georgios Scheiner-Bobis
1. Structure determination of a bacterial ZIP in lipidic cubic phase
Jian Hu
2. Cell-based metal transport assay for animal and plant ZIPs
Jian Hu
3. Measurement of metal binding affinity and transport activity in ZIP transporters using spectroscopic methods
Bharati Mitra
4. Considerations in production of the (prokaryotic) ZIP family transporters for sturcural and functional studies
Cheng Ma
5. Metal-protein interactions investigated using model systems: thermodynamic, spectroscopic and computational methods
Maurizio Remelli
6. Expression, purification and crystallization of the extracellular domain of a mammalian ZIP4
Tuo Zhang
7. Getting Zinc Into and Out of Cells
Kenneth Merz
8. In-silico modeling of ZIP9 (TBD)
Georgios Scheiner-Bobis
Unit II. Cellular Function and Regulation
9. Methods to visualise zinc transporter proteins of the SLC39A family in cells
Kathryn M. Taylor
10. Assessing metal ion transporting activity of ZIPs: intracellular zinc and iron detection
Zhou Bing
11. In vitro studies for studying manganese transport and homeostasis
Young Ah Seo
12. In vitro studies to evaluate zinc transport ability of ZIPs through analyzing ZNT1 and MT expression responses
Taiho Kambe
13. The optimized method to detect mouse erythrocyte membrane ZIP metal transporters by immunoblotting analysis
Ningning Zhao
14. Dynamic internalization and trafficking of zinc transporter
Chi Zhang
15. Assessing the impact of varying zinc levels in the transcription profiles of human neurons
Robert E. Dempski
16. Quantifying homomeric and heteromeric ZIP complexes using fluorescence correlation and fluorescence cross-correlation spectroscopy
Robert E. Dempski
17. ZIP9-directed androgenic tetra peptides (TBD)
Georgios Scheiner-Bobis
- Edition: 1
- Volume: 687
- Published: August 31, 2023
- Imprint: Academic Press
- Language: English
JH
Jian Hu
Prof. Jian Hu is a structural biologist and protein chemist with 12 years of research experience and excellent track record in prestigious academic institutions. He has conducted structure-function research on different proteins, especially membrane proteins, by employing a wide variety of biophysical and biochemical approaches.
He is currently an Associate Professor in Michigan State University, College of Natural Science
Affiliations and expertise
Associate Professor, College of Natural Science, Michigan State University, MI, USARead ZIP Metal Ion Transporters on ScienceDirect