Viral Replication Enzymes and their Inhibitors Part A

1st Edition, Volume 49 - October 22, 2021
Editors: Craig E. Cameron, Jamie J. Arnold
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 2 3 4 6 8 - 6
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 2 3 4 6 9 - 3

Viral Replication Enzymes and their Inhibitors Part A, Volume 49, the latest release in the Enzymes series, highlights new advances in the field, with this new volume presen… Read more

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Viral Replication Enzymes and their Inhibitors Part A, Volume 49, the latest release in the Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics.

Cover image
Title page
Table of Contents
Copyright
Contributors
Preface
Chapter One: CoV-er all the bases: Structural perspectives of SARS-CoV-2 RNA synthesis
Abstract
1: Introduction
2: Coronavirus genome organization
3: The RNA-dependent RNA polymerase
4: Concerted enzymatic functions vital to replication/transcription
5: Antivirals targeting the RdRp
6: Conclusion
References
Chapter Two: Mechanisms of inhibition of viral RNA replication by nucleotide analogs
Abstract
1: Mechanistic basis for effective inhibition
2: Incorporation versus excision of nucleotide analogs
3: Toxicity
4: SARS CoV-2 RdRp kinetics and mechanism
5: Structural basis for delayed inhibition by Remdesivir
6: Role of the proofreading exonuclease in effectiveness of nucleotide analogs
7: Summary and future directions
References
Chapter Three: HCV RdRp, sofosbuvir and beyond
Abstract
1: Introduction
2: Part I: HCV RdRp biochemistry, structure biology, and modulation by viral and human proteins
3: Part II: HCV drug discovery targeting HCV NS5B
References
Chapter Four: Understanding viral replication and transcription using single-molecule techniques
Abstract
1: Introduction
2: Single-molecule FRET of influenza A virus RNA synthesis
3: Single-molecule analysis of HIV-I reverse transcriptase activity
4: Analysis of hepatitis C virus RNA and helicase function
5: Single-molecule observation of DNA phage replication
6: Single-molecule analysis of polymerase fidelity
7: Conclusions
References
Chapter Five: In vitro single-molecule manipulation studies of viral DNA replication
Abstract
1: Introduction
2: In vitro single molecule manipulation methods: Optical and magnetic tweezers
3: Mechanical properties of single- and double-stranded DNA
4: Manipulation of single SSB-ssDNA complexes
5: Mechanical tension as a variable to study the real-time kinetics of primer extension DNA synthesis
6: Mechanical force and mechano-chemistry: Identification of the translocation step of DNA polymerases
7: Mechanical tension as a variable to study strand displacement DNA synthesis
8: Mechanical tension as a variable to study DNA unwinding by helicases
9: When the helicase has company: Mechanical manipulation of several proteins at the fork
10: Summary and conclusions
Acknowledgments
References
Chapter Six: Allosteric and dynamic control of RNA-dependent RNA polymerase function and fidelity
Abstract
1: Brief introduction to the structure and function of RNA-dependent RNA polymerases
2: NMR and MD methods to evaluate protein structure and dynamics
3: Protein conformational dynamics in the catalytic cycle of RNA-dependent RNA polymerases
4: Dynamics and allostery important for the fidelity of nucleotide incorporation
5: Dynamics and allostery important for anti-viral drug development
6: Conclusions
Acknowledgments
References
Chapter Seven: Single-cell analysis for the study of viral inhibitors
Abstract
1: Introduction
2: Microfluidics: A powerful tool for single-cell virology
3: Study of antivirals at single-cell resolution
4: Viral transmission dynamics at the single-cell level
5: Future of single-cell virology
Acknowledgments
References
Chapter Eight: Structural basis of viral RNA-dependent RNA polymerase nucleotide addition cycle in picornaviruses
Abstract
1: Introduction
2: Structural basis of picornaviral RdRP NAC
3: Perspective
References
Chapter Nine: Picornaviral 2C proteins: A unique ATPase family critical in virus replication
Abstract
1: Introduction
2: The function of picornaviral 2C protein
3: RNA binding activity
4: The roles of picornaviral 2C protein in the virus lifecycle
5: Structural characterizations of picornaviral 2C protein
6: Inhibitors of picornaviral 2C protein
7: Perspectives
References
Chapter Ten: Flavivirus enzymes and their inhibitors
Abstract
1: Introduction
2: Flavivirus replication
3: Flavivirus NS3 protein
4: Flavivirus NS5 protein
5: Concluding remarks
References
Chapter Eleven: Watching the bacterial RNA polymerase transcription reaction by time-dependent soak-trigger-freeze X-ray crystallography
Abstract
Acknowledgments
References
Chapter Twelve: Inhibition of viral RNA-dependent RNA polymerases with clinically relevant nucleotide analogs
Abstract
1: Introduction
2: Viral RNA-dependent RNA polymerases
3: Picornaviruses
4: Hepatitis C virus
5: Respiratory syncytial virus
6: Coronaviruses
7: Influenza viruses
References

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Viral Replication Enzymes and their Inhibitors Part A

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Craig E. Cameron

Jamie J. Arnold

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