Tissue Culture

List of ContributorsPrefaceSection I. Primary Tissue Dissociation Chapter 1. Pronase Chapter 2. Trypsin A. Mammalian Tissues B. High Yield Method for Kidney Tissue C. Marine Teleost Fish Tissues D. Amphibian Tissues Chapter 3. Collagenase Treatment of Chick Heart and Thyroid Chapter 4. Sequential Enzyme Treatment of Mouse Mammary Gland Chapter 5. Nonenzymatic Dissociations A. Leukocyte Cell Separation on Glass B. Specific Cell Fractionation on Chemically Derivatized SurfacesSection II. Preparation of Primary Cultures Chapter 1. Human Skin Fibroblasts Chapter 2. Fetal Human Diploid Cells Chapter 3. Human Mammary Tumors Chapter 4. Human Glia Cells Chapter 5. Human Liver Cells Chapter 6. Human Blood Leukocytes Chapter 7. Amniocentesis Chapter 8. Human Lymph Nodes Chapter 9. Human Biopsy Material from Genetic Abnormalities Chapter 10. Mammalian Myocardial Cells Chapter 11. Mammalian Glomerular Cells Chapter 12. Organotypic Mammalian Central and Peripheral Nerve Tissue Chapter 13. Marmoset Bone Marrow Chapter 14. Monkey Kidney Cells Chapter 15. Rabbit Kidney and Skin Chapter 16. Rat Skeletal Muscle Cells Chapter 17. Rat Pituitary Explants Chapter 18. Chick Embryo Cells Chapter 19. Frog Embryos ( Haploid Lines) Chapter 20. Adult Amphibian Tissues and Leukocytes Chapter 21. Marine Teleost Fish Tissues Chapter 22. Fish Lymphocytes and Blastogenesis Chapter 23. Freshwater Fishes Chapter 24. Goldfish Tissues Chapter 25. Reptiles Chapter 26. Dipteran Embryos and Larvae (Diploid Lines) Chapter 27. Cockroach and Grasshopper Embryo Tissue Chapter 28. Plants: Haploid Tissue Cultures Chapter 29. Aspen Callus Chapter 30. Plant Embryos Chapter 31. Somatic Plant Cells Chapter 32. Roots Chapter 33. LeavesSection III. Cell Harvesting Chapter 1. Pronase Chapter 2. Trypsinization of Diploid Human Fibroblasts Chapter 3. Transplantable Rat Tumors and Cold Temperature Release Chapter 4. Cells at Defined Stages of the Cell Cycle A. Selection of Mitotic Cells from Monolayer Culture B. Sedimentation in Sucrose and Ficoll Gradients of Cells Grown in Suspension Culture Chapter 5. Processing Cells for Enzyme AssaysSection IV. Replicate Culture Methods Chapter 1. Monolayer Cultures Chapter 2. Plant Cell Suspension Cultures Chapter 3. Subculturing Human Diploid Fibroblast Cultures Chapter 4. Apparatus for Changing Tissue Culture Media Chapter 5. Single "Subculture" in Situ Using Glass Beads Chapter 6. Maintenance of Diploid Fibroblast Cultures as Nonmitotic PopulationsSection V. Single Cell Isolations and Cloning Chapter 1. Animal Cells A. Capillary Techniques B. Microdrop Techniques Chapter 2. Plant Cells Chapter 3. Dilution Plating and Nutritional Considerations A. Animal Cells B. Plant Cells Chapter 4. Dilution Plating on Coverslip Fragments Chapter 5. Microtest Plates A. Single Cell Clones B. Replica Plating Chapter 6. Low Oxygen Tension Technique Chapter 7. Soft Agar TechniquesSection VI. Perfusion and Mass Culture Techniques Chapter 1. Dual-Rotary Circumfusion System Chapter 2. Automated Single Rose Chamber for Cardiac and Other Cells Chapter 3. Culture Dish Perfusion with Cinemicrography Chapter 4. Sykes-Moore Chamber Chapter 5. Culture of the Lens (33°) Chapter 6. Cardiac Cells in a Capillary Tube Chapter 7. Artificial Capillaries: An Approach to Tissue Growth in Vitro Chapter 8. Replicate Roller Bottles Chapter 9. Replicate Tube Chambers for Bones Chapter 10. Multisurface Stacked Plate Propagators Chapter 11. Bulk Culture of Cell Monolayers Chapter 12. Spin Filter Device for Suspension Cultures Chapter 13. Automation of Continuous Suspension Cultures by Means of a Nephelostat Chapter 14. A Chemostat for Single Cell Cultures of Higher PlantsSection VII. Cell Propagation on Miscellaneous Culture Supports Chapter 1. Collagen-Coated Cellulose Sponge Chapter 2. Microcarrier Cultures of Animal Cells Chapter 3. Polyester Sheeting Chapter 4. Protein Polymers as a Substratum for the Modulation of Cell Proliferation in Vitro Chapter 5. Titanium Disks Chapter 6. Filter Paper Supports for Plant CulturesSection VIII. Evaluation of Culture Dynamics Chapter 1. Hemocytometer Counting Chapter 2. Electronic Enumeration and Sizing of Cells A. Primary Tissue Cells B. Tissue Culture Cells Chapter 3. Dye Exclusion Tests for Cell Viability Chapter 4. Quantitative Methods for Measuring Cell Growth and Death Chapter 5. Cell Cycle Analysis A. Mammalian Cells B. Control and Analysis of the Mitotic Cycle in Cultured Plant Root MeristemsSection IX. Recent Techniques Facilitating Microscopic Observation of Cells Chapter 1. Contrast Methods in the Microscopy of Living Tissue Chapter 2. Rapid Embedding of Cell Culture Monolayers and Suspensions Chapter 3. Embedding in Situ Chapter 4. Vertical Sectioning A. Cells on Millipore Filters B. Cells in Plastic Flasks C. Cells Grown on Decalcified Eggshell Membrane with Special Reference to Electron Microscopic Study Chapter 5. Hydraulically Operated Microscope Stage Chapter 6. Culture Dish for High Resolution MicroscopySection X. Cell Hybridization Chapter 1. Somatic Hybridization in Studies of Heredity of Cell Malignancy Chapter 2. Production and Characterization of Proliferating Somatic Cell Hybrids Chapter 3. Plant Protoplast CultureSection XI. Virus Propagation and Assay Chapter 1. Microculture Procedures A. Vesicular Stomatitis Virus B. Simian Virus 40 C. Antibody Neutralization Surveys Chapter 2. Quantal and Enumerative Titration of Virus in Cell Cultures Chapter 3. Diagnosis of Virus-Infected Cultures with Fluorescein-Labeled Antisera Chapter 4. Rapid Method for Detection of Budding Virus by Electron Microscopy Chapter 5. Monitoring for Presence of Oncogenic Virus in Tissue Culture Chapter 6. Tobacco Mosaic Virus in Plant Tissue CultureSection XII. Production of Hormones and Intercellular Substances Chapter 1. Growth Hormone and Prolactin from Rat Pituitary Tumor Cells Chapter 2. Enhancement of Growth Hormone Production by Cortisol Chapter 3. Human Chorionic Gonadotropin Chapter 4. Parathyroid Hormone and Calcitonin Chapter 5. Measurement of Collagen Synthesis Chapter 6. Evaluation of Milk Protein Synthesis Chapter 7. Interferon Yield versus Cell Genotype Chapter 8. ImmunoglobulinsSection XIII. Diagnosis and Understanding of Disease Chapter 1. Some Cell Culture Procedures in Diagnostic Medical Virology Chapter 2. Diagnostic Use of Cell Cultures Initiated from Amniocentesis Chapter 3. Expressions of Citrullinemia in Skin Fibroblast Cultures Chapter 4. The Hurler Cell in Culture Chapter 5. Characteristics of Fibroblasts Cultured from Patients with Galactosemia or Galactokinase Deficiency Chapter 6. Evaluation of Amino Acid Metabolism in Maple Syrup Urine Disease Chapter 7. Malignant Transformation of Cells in Culture Using Oncogenic Chemicals Chapter 8. Malignant Transformation of Cells in Culture Chapter 9. Some in Vitro Models of Normal and Malignant Cell Interaction Chapter 10. Growth and Isolation of Nutritional VariantsSection XIV. Quality Control Measures Chapter 1. Glassware Preparation, Sterilizations, and Use of Laminar Flow Systems Chapter 2. Purification and Standardization of Water for Tissue Culture Chapter 3. A Surveillance Procedure Applied to Sera Chapter 4. Preparation of Sera for Nutritional Studies Chapter 5. Sample Preparations of Media A. Vertebrate Cells B. Invertebrate Cells C. Plant Cultures Chapter 6. Determination and Survey of Osmolality in Culture Media Chapter 7. Control of Culture pH with Synthetic Buffers Chapter 8. Freezing, Storage, and Recovery of Cell Stocks Chapter 9. Detection, Elimination, and Prevention of Bacteria and Fungi in Tissue Cultures Chapter 10. Screening Tissue Cultures for Mycoplasma Infections Chapter 11. Mycoplasma Contamination of Cell Cultures: Incidence, Source, Prevention, and Problems of Elimination Chapter 12. Elimination of Pathogens from Shoot Tip Cultures Chapter 13. Contamination of Tissue Culture and Its Cure: Some Examples Chapter 14. Serological Identifications of Cells in Culture A. Animal Cells by Fluorescent-Labeled Antibody B. Poikilotherm Cell Identity by Cytotoxic Antibody Test C. Invertebrate Cell Cultures D. Detection of HL-A Antigens on Cultured Normal Human Diploid Fibroblasts Chapter 15. Karyology of Cells in Culture A. Preparation and Analysis of Karyotypes and Idiograms B. Harvesting Human Leukocyte Cultures for Chromosome Cytology C. Constitutive Heterochromatin (C-Bands) D. Fluorescent Banding of Chromosomes (Q-bands) E. Trysin Technique to Reveal G-Bands F. Characteristics of Insect Cells G. Characteristics of Plant Cells Chapter 16. Biochemical Identification of Cells in Culture A. Human Cell Lines by Enzyme Polymorphism B. Enzymatic "Fingerprinting" Chapter 17. Introduction to Cytoenzymological Methods and Isozymes Chapter 18. Transport and Shipping of Cultures, Including International RegulationsAuthor IndexSubject Index