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Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics
- 9th Edition - September 2, 2023
- Editor: Nader Rifai
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 3 5 8 3 - 8
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 3 6 9 3 - 4
**Selected for Doody’s Core Titles® 2024 with "Essential Purchase" designation in Laboratory Technology**Master clinical lab testing skills with the condensed version of the Tietz… Read more
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Request a sales quote**Selected for Doody’s Core Titles® 2024 with "Essential Purchase" designation in Laboratory Technology**
Master clinical lab testing skills with the condensed version of the Tietz Textbook! Designed for use by CLS students, Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 9th Edition provides a streamlined guide to the clinical chemistry knowledge you need to work in a real-world, clinical lab. Coverage ranges from laboratory principles to analytical techniques and instrumentation, analytes, pathophysiology, and more. New content keeps you current with the latest developments in molecular diagnostics. From highly respected clinical chemistry educator Nader Rifai, this textbook shows how to select and perform diagnostic lab tests, and how to accurately evaluate results.
- Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing.
- Authoritative, foundational content mirrors that in the Tietz "bible" of laboratory medicine but in a more concise way.
- Updated chapters on molecular diagnostics cover the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field.
- Clinical cases from the Coakley Collection demonstrate how concepts from the text are applied in real-life scenarios.
- More than 400 illustrations and easy-to-read summary tables help you better understand and remember key concepts.
- Learning objectives, key words with definitions, and review questions are included in each chapter to make learning easier.
- Instructions for online access
- Cover image
- Title page
- Table of Contents
- Copyright
- Associate Editors
- Contributors
- Preface
- Part I. Principles of Laboratory Medicine
- 1. Clinical Chemistry and Molecular Diagnostics
- LOOKING BACK
- EXPANDING BOUNDARIES DEFINED BY TECHNOLOGY
- HOW IS CLINICAL CHEMISTRY PRACTICED?
- GUIDING PRINCIPLES OF PRACTICING THE PROFESSION
- CONFIDENTIALITY OF PATIENT INFORMATION
- ALLOCATION OF RESOURCES
- CODES OF CONDUCT
- PUBLISHING ISSUES
- CONFLICTS OF INTEREST
- WHAT IS IN THIS TEXTBOOK?
- 2. Analytical and Clinical Evaluation of Methods
- ASSAY SELECTION OVERVIEW
- BASIC STATISTICS
- TECHNICAL VALIDITY OF ANALYTICAL ASSAYS
- Assay Comparison
- MONITORING SERIAL RESULTS
- Traceability
- DIAGNOSTIC ACCURACY OF LABORATORY TESTS
- 3. Preanalytical Variation
- PREANALYTICAL PHASE
- ENDOGENOUS INTERFERENCE FACTORS
- Exogenous Interference Factors
- SPECIFIC CONSIDERATIONS
- 4. Biological Variation and Analytical Performance Specifications
- NATURE OF BIOLOGICAL VARIATION AND CENTRAL CONCEPTS
- GENERATION OF DATA ON COMPONENTS OF BIOLOGICAL VARIATION
- QUALITY OF BIOLOGICAL VARIATION DATA AND DATABASES
- BIOLOGICAL VARIATION IN DIFFERENT STATES OF HEALTH AND SETTINGS
- INTERPRETATION AND USE OF DATA
- ANALYTICAL PERFORMANCE SPECIFICATIONS
- OVERALL CONCLUSIONS
- 5. Establishment and Use of Reference Intervals
- ESTABLISHMENT OF REFERENCE INTERVALS
- USE OF REFERENCE INTERVALS
- 6. Specimen Collection, Processing, and Other Preanalytical Variables
- PATIENT IDENTIFICATION
- TYPES OF SPECIMENS
- HANDLING OF SPECIMENS FOR ANALYSIS
- MAINTENANCE OF SPECIMEN IDENTIFICATION
- CONCLUSION
- 7. Quality Control of the Analytical Examination Process
- MEASUREMENT PROCEDURE PERFORMANCE AS A PREREQUISITE FOR A QUALITY CONTROL PLAN
- DEVELOPING A QUALITY CONTROL PLAN AND IMPLEMENTING INTERNAL QUALITY CONTROL PROCEDURES
- EXTERNAL QUALITY ASSESSMENT OR PROFICIENCY TESTING
- 8. Principles of Basic Techniques and Laboratory Safety
- CHEMICALS
- REFERENCE MATERIALS
- BASIC TECHNIQUES AND PROCEDURES
- SAFETY
- Part II. Analytical Techniques and Instrumentation
- 9. Optical Techniques
- NATURE OF LIGHT
- SPECTROPHOTOMETRY
- Instrumentation
- REFLECTANCE PHOTOMETRY
- FLAME EMISSION AND INDUCTIVELY COUPLED PLASMA SPECTROPHOTOMETRY
- FLUOROMETRY
- CHEMILUMINESCENCE, BIOLUMINESCENCE, AND ELECTROCHEMILUMINESCENCE
- NEPHELOMETRY AND TURBIDIMETRY
- 10. Electrochemistry and Chemical Sensors
- POTENTIOMETRY
- ION-SELECTIVE MEMBRANE ELECTRODES
- VOLTAMMETRY AND AMPEROMETRY
- CONDUCTOMETRY
- COULOMETRY
- Biosensors
- 11. Electrophoresis
- BASIC CONCEPTS
- THEORY OF ELECTROPHORESIS
- CLINICAL ELECTROPHORESIS
- CAPILLARY ELECTROPHORESIS
- TYPES OF ELECTROPHORESIS
- SPECIALTY ELECTROPHORESIS TECHNIQUES
- 12. Chromatography
- BASIC PRINCIPLES OF CHROMATOGRAPHY
- GAS CHROMATOGRAPHY
- LIQUID CHROMATOGRAPHY
- PLANAR CHROMATOGRAPHY
- 13. Mass Spectrometry
- BASIC CONCEPTS
- INSTRUMENTATION
- CLINICAL APPLICATIONS
- 14. Enzyme and Rate Analyses
- ENZYME NOMENCLATURE
- ENZYMES AS PROTEINS
- SPECIFICITY AND THE ACTIVE CENTER
- ISOENZYMES AND OTHER MULTIPLE FORMS OF ENZYMES
- ENZYMES AS CATALYSTS
- UNITS FOR EXPRESSING ENZYME ACTIVITY
- ENZYME KINETICS
- FACTORS GOVERNING THE RATE OF ENZYME-CATALYZED REACTIONS
- ANALYTICAL ENZYMOLOGY
- 15. Immunochemical Techniques
- BASIC CONCEPTS AND DEFINITIONS
- ANTIGEN-ANTIBODY BINDING
- QUALITATIVE METHODS
- QUANTITATIVE METHODS
- CELL-BASED AND TISSUE-BASED IMMUNOCHEMICAL TECHNIQUES
- 16. Automation in the Clinical Laboratory
- NONANALYTICAL AUTOMATION FOR THE CLINICAL LABORATORY
- PRACTICAL CONSIDERATIONS
- AUTOMATION OF THE ANALYTICAL PROCESSES
- AREAS OF ANALYTICAL AUTOMATION
- 17. Point-of-Care Testing
- TECHNOLOGY
- QUALITY MANAGEMENT
- EVIDENCE OF EFFECTIVENESS
- Part III. Analytes
- 18. Amino Acids, Peptides, and Proteins
- AMINO ACIDS
- PEPTIDES
- PROTEINS
- PROTEIN CATABOLISM
- 19. Serum Enzymes
- BASIC CONCEPTS
- MUSCLE ENZYMES
- LIVER ENZYMES
- Pancreatic Enzymes
- OTHER CLINICALLY IMPORTANT ENZYMES
- 20. Tumor Markers
- CANCER—AN OVERVIEW
- HISTORICAL BACKGROUND
- GENERAL PRINCIPLES GUIDING THE USE OF ALL TUMOR MARKERS
- QUALITY REQUIREMENTS FOR TUMOR MARKER USE IN THE CLINIC
- Use of Tumor Markers in Specific Malignancies
- CONCLUSION
- 21. Kidney Function Tests
- PROTEINURIA: TOTAL PROTEIN AND ALBUMIN
- CREATININE
- CYSTATIN C
- UREA
- URIC ACID (URATE)
- ASSESSMENT OF KIDNEY FUNCTION: GLOMERULAR FILTRATION RATE
- 22. Carbohydrates
- CHEMISTRY OF CARBOHYDRATES
- BIOCHEMISTRY AND PHYSIOLOGY
- ANALYTICAL METHODOLOGY
- 23. Lipids and Lipoproteins
- INTRODUCTION
- LIPID BIOCHEMISTRY
- LIPOPROTEINS
- LIPOPROTEIN METABOLISM
- INHERITED DISORDERS OF LIPOPROTEIN METABOLISM
- CLINICAL MANAGEMENT OF LIPOPROTEIN DISORDERS
- ANALYSIS OF LIPIDS, LIPOPROTEINS, AND APOLIPOPROTEINS
- ADVANCED LIPID AND LIPOPROTEIN TESTING FOR ASCVD RISK ASSESSMENT
- 24. Electrolytes and Blood Gases
- ELECTROLYTES
- SODIUM
- POTASSIUM
- METHODS FOR THE DETERMINATION OF SODIUM AND POTASSIUM
- ELECTROLYTE EXCLUSION EFFECT
- Chloride
- METHODS FOR DETERMINATION OF CHLORIDE IN BODY FLUIDS
- SWEAT CHLORIDE
- BICARBONATE (TOTAL CARBON DIOXIDE)
- PRINCIPLES OF OSMOTIC PRESSURE AND OSMOSIS
- BLOOD GASES AND pH
- BEHAVIOR OF GASES
- APPLICATION OF THE HENDERSON-HASSELBALCH EQUATION IN BLOOD GAS MEASUREMENTS
- OXYGEN IN BLOOD
- HEMOGLOBIN OXYGEN SATURATION
- MEASUREMENT OF pCO2, pO2, and pH
- TEMPERATURE CORRECTION OF MEASURED pH, pCO2, and pO2
- 25. Hormones
- BACKGROUND
- SYNTHESIS, RELEASE, AND GENERAL ACTION OF HORMONES
- CELL SIGNALING MECHANISMS OF HORMONE RECEPTORS
- SIGNAL TRANSDUCTION FROM CELL SURFACE G-PROTEIN–COUPLED RECEPTORS
- SIGNAL TRANSDUCTION FROM CELL SURFACE ENZYME-COUPLED RECEPTORS
- CLINICAL DISORDERS OF HORMONE ACTION
- TECHNIQUES FOR MEASUREMENT OF HORMONES AND RELATED ANALYTES
- 26. Catecholamines and Serotonin
- BACKGROUND
- CHEMISTRY, BIOSYNTHESIS, RELEASE, AND METABOLISM
- PHYSIOLOGY OF CATECHOLAMINE AND SEROTONIN SYSTEMS
- CLINICAL APPLICATIONS
- ANALYTICAL METHODOLOGY
- 27. Vitamins and Trace Elements
- VITAMIN AND TRACE ELEMENT STATUS
- VITAMINS
- TRACE ELEMENTS
- LABORATORY ASSESSMENT OF TRACE ELEMENT STATUS
- ANALYTICAL METHODS FOR MEASURING TRACE ELEMENTS
- 28. Hemoglobin and Iron
- HEMOGLOBIN
- IRON
- 29. Porphyrins and Porphyrias
- PORPHYRIN CHEMISTRY
- HEME BIOSYNTHESIS
- REGULATION OF HEME BIOSYNTHESIS
- SOLUBILITY AND EXCRETION OF HEME PRECURSORS
- THE PORPHYRIAS
- LABORATORY DIAGNOSIS OF PORPHYRIA
- LABORATORY ANALYSIS
- 30. Therapeutic Drug Monitoring
- FUNDAMENTAL PRINCIPLES OF APPLIED PHARMACOKINETICS
- THERAPEUTIC DRUG MONITORING OF DRUGS IN COMMON CLINICAL USE
- 31. Clinical Toxicology
- INTRODUCTION
- SCREENING PROCEDURES FOR DETECTION OF SUBSTANCES
- PHARMACOLOGY AND ANALYSIS OF SPECIFIC DRUGS AND TOXIC AGENTS
- AGENTS THAT CAUSE CELLULAR HYPOXIA
- ALCOHOLS OF TOXICOLOGICAL INTEREST
- ANALGESICS (NONPRESCRIPTION)
- AGENTS RELATED TO ANTICHOLINERGIC TOXIDROME
- Agents Related to Cholinergic Toxidrome
- Substances of Use/Misuse
- Drug-Facilitated Crimes
- Pain Management
- Detection of Substances of Use/Misuse Using Other Types of Specimens
- 32. Toxic Elements
- PREVALENCE OF ELEMENTAL TOXICITIES
- DIAGNOSING TOXICITY
- TREATMENT
- SPECIFIC ELEMENTS
- Part IV. Pathophysiology
- 33. Diabetes Mellitus
- CLASSIFICATION
- HORMONES THAT REGULATE BLOOD GLUCOSE CONCENTRATION
- CLINICAL UTILITY OF MEASURING INSULIN, PROINSULIN, C-PEPTIDE, AND GLUCAGON
- PATHOGENESIS OF TYPE 1 DIABETES MELLITUS
- PATHOGENESIS OF TYPE 2 DIABETES MELLITUS
- MONOGENIC DIABETES MELLITUS
- DIAGNOSIS OF NON-GESTATIONAL DIABETES MELLITUS
- DIAGNOSIS OF GESTATIONAL DIABETES MELLITUS
- CHRONIC COMPLICATIONS OF DIABETES MELLITUS
- SELF-MONITORING OF BLOOD GLUCOSE
- CONTINUOUS GLUCOSE MONITORING
- NON-INVASIVE GLUCOSE MONITORING
- GLYCATED HEMOGLOBIN
- 1,5-ANHYDROGLUCITOL
- ALBUMINURIA
- 34. Cardiac Function
- BACKGROUND
- ANATOMY AND PHYSIOLOGY OF THE HEART
- CARDIAC DISEASE
- CARDIAC BIOMARKERS
- B-TYPE NATRIURETIC PEPTIDE AND CONGESTIVE HEART FAILURE
- ANALYTICAL CONSIDERATIONS
- 35. Kidney Disease
- INTRODUCTION
- ANATOMY
- KIDNEY FUNCTION
- KIDNEY PHYSIOLOGY
- PATHOPHYSIOLOGY OF KIDNEY DISEASE
- DISEASES OF THE KIDNEY
- KIDNEY REPLACEMENT THERAPY
- 36. Physiology and Disorders of Water, Electrolyte, and Acid-Base Metabolism
- INTRODUCTION
- WATER AND ELECTROLYTES—COMPOSITION OF BODY FLUIDS
- EXTRACELLULAR AND INTRACELLULAR COMPARTMENTS
- ELECTROLYTES
- SODIUM
- POTASSIUM
- CHLORIDE
- ACID-BASE PHYSIOLOGY
- ACID-BASE BALANCE AND ACID-BASE STATUS
- BUFFER SYSTEMS AND THEIR ROLE IN REGULATING THE pH OF BODY FLUIDS
- RESPIRATORY MECHANISM IN THE REGULATION OF ACID-BASE BALANCE
- RENAL MECHANISMS IN THE REGULATION OF ACID-BASE BALANCE
- CONDITIONS ASSOCIATED WITH ABNORMAL ACID-BASE STATUS AND ABNORMAL ELECTROLYTE COMPOSITION OF BLOOD
- METABOLIC ALKALOSIS (PRIMARY BICARBONATE EXCESS)
- RESPIRATORY ACIDOSIS
- RESPIRATORY ALKALOSIS
- 37. Liver Disease
- ANATOMY OF THE LIVER
- BIOCHEMICAL FUNCTIONS OF THE LIVER
- DISEASES OF THE LIVER
- LIVER TRANSPLANTATION
- 38. Gastric, Intestinal, and Pancreatic Function
- INTRODUCTION TO THE ANATOMY AND PHYSIOLOGY OF THE GASTROINTESTINAL TRACT
- STOMACH: DISEASES AND LABORATORY INVESTIGATIONS
- INTESTINAL DISORDERS AND THEIR LABORATORY INVESTIGATION
- PANCREAS: DISEASE AND ASSESSMENT OF EXOCRINE PANCREATIC FUNCTION
- 39. Bone and Mineral Metabolism
- OVERVIEW OF SKELETAL METABOLISM
- BIOCHEMICAL MEASUREMENTS IN METABOLIC BONE DISEASE
- 40. Disorders of the Pituitary Gland
- BACKGROUND
- CONTENT
- ANATOMY
- HYPOTHALAMIC REGULATION
- SUMMARY OF PITUITARY-RELATED DISORDERS
- 41. Adrenal Cortex
- INTRODUCTION
- ADRENAL ANATOMY AND PHYSIOLOGY
- ADRENAL STEROID BIOCHEMISTRY
- REGULATION OF ADRENOCORTICAL HORMONES
- ADRENOCORTICAL INSUFFICIENCY
- LABORATORY INVESTIGATION OF ADRENOCORTICAL DYSFUNCTION
- SUMMARY
- List of Abbreviations
- 42. Thyroid Disorders
- ANATOMY
- THYROID HORMONES
- IODINE AND THYROID DISORDERS
- EXTRATHYROIDAL FACTORS THAT AFFECT THYROID FUNCTION
- NONTHYROIDAL ILLNESS
- EVALUATION OF THYROID FUNCTION
- THYROID DISEASE
- THYROID DISORDERS IN PREGNANCY AND POSTPARTUM
- THYROID NEOPLASIA
- THYROID DISEASE IN CHILDREN
- GENETICS
- 43. Reproductive Endocrinology and Related Disorders
- BACKGROUND
- MALE REPRODUCTIVE BIOLOGY
- FEMALE REPRODUCTIVE BIOLOGY
- INFERTILITY
- ANALYTIC METHODS FOR REPRODUCTIVE HORMONES
- 44. Pregnancy and Prenatal Testing
- BACKGROUND
- HUMAN PREGNANCY
- MATERNAL ADAPTATION
- FUNCTIONAL DEVELOPMENT OF THE FETUS
- MATERNAL AND FETAL HEALTH ASSESSMENT
- COMPLICATIONS OF PREGNANCY
- PRENATAL SCREENING FOR FETAL DEFECTS
- PRENATAL SCREENING TESTS
- ANALYTICAL METHODOLOGY
- 45. Newborn Screening and Inborn Errors of Metabolism
- BACKGROUND
- INHERITANCE PATTERN OF METABOLIC DISORDERS
- NEWBORN SCREENING
- INBORN ERRORS OF METABOLISM
- DIAGNOSTIC TESTS FOR INHERITED DISORDERS OF METABOLISM
- 46. Pharmacogenetics
- PRINCIPLES OF PHARMACOGENETICS
- SPECIFIC EXAMPLES OF PHARMACOGENE ASSOCIATIONS
- FUTURE DIRECTIONS
- Part V. Molecular Diagnostics
- 47. Principles of Molecular Biology
- BACKGROUND
- MOLECULAR BIOLOGY ESSENTIALS
- NUCLEIC ACID STRUCTURE AND FUNCTION
- CENTRAL DOGMA OF MOLECULAR BIOLOGY
- EPIGENETICS
- SUMMARY
- 48. Genomes, Variants, and Massively Parallel Methods
- BACKGROUND
- HUMAN GENOME
- Single Nucleotide Variants
- NOMENCLATURE
- MASSIVELY PARALLEL METHODS
- INFORMATICS
- 49. Molecular Microbiology
- BACKGROUND
- OVERVIEW OF METHODOLOGIES FOR THE DIAGNOSIS OF INFECTIOUS DISEASES
- TARGET NUCLEIC ACID AMPLIFICATION STRATEGIES
- NON-PCR TARGET AND SIGNAL AMPLIFICATION APPROACHES
- SEQUENCING
- MASS SPECTROMETRY
- HEALTH CARE-ASSOCIATED INFECTIONS
- SYNDROMIC PANELS
- DIRECT PATHOGEN DETECTION
- UPPER RESPIRATORY TRACT INFECTIONS
- PNEUMONIA PANELS
- POINT-OF-CARE TESTING
- EMERGING TECHNOLOGIES
- CRISPR-CAS APPLICATIONS
- Reference Information
- APPENDIX. Reference Information for the Clinical Laboratory
- ANSWERS
- INDEX
- No. of pages: 1072
- Language: English
- Edition: 9
- Published: September 2, 2023
- Imprint: Elsevier
- Hardback ISBN: 9780323935838
- eBook ISBN: 9780323936934
NR
Nader Rifai
Affiliations and expertise
Professor Department of Pathology Harvard Medical School; The Louis Joseph Gay-Lussac Chair in Laboratory Medicine Boston Children's Hospital; Director of Clinical Chemistry Laboratory Medicine Boston Children's Hospital Boston, Massachusetts