Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics
- 8th Edition - October 31, 2018
- Author: Nader Rifai
- Language: English
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 5 4 9 7 3 - 8
Get the foundational knowledge you need to successfully work in a real-world, clinical lab with Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Editi… Read more
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Request a sales quoteGet the foundational knowledge you need to successfully work in a real-world, clinical lab with Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition. From highly respected clinical chemistry expert Nader Rifai, this condensed, easier-to-understand version of the acclaimed Tietz Textbook of Clinical Chemistry and Molecular Diagnostics uses a laboratory perspective to guide you through selecting and performing diagnostic lab tests and accurately evaluating the results. Coverage includes laboratory principles, analytical techniques, instrumentation, analytes, pathophysiology, and more. This eighth edition features new clinical cases from The Coakley Collection, new questions from The Deacon’s Challenge of Biochemical Calculations Collection, plus new content throughout the text to ensure you stay ahead of all the latest techniques, instrumentation, and technologies.
- Condensed version of the clinical chemistry "bible" offers the same authoritative and well-presented content in a much more focused and streamlined manner.
- Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing.
- Updated chapters on molecular diagnostics cover the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field.
- Learning objectives, key words, and review questions are included in each chapter to support learning.
- More than 500 illustrations plus easy-to-read tables help readers better understand and remember key concepts.
- Instructions for online access
- Cover image
- Title page
- Table of Contents
- Copyright
- Associate Editors
- Contributors
- Preface
- Part I. Principles of Laboratory Medicine
- 1. Clinical Chemistry and Molecular Diagnostics
- Looking Back
- Expanding Boundaries Defined By Technology
- How is Clinical Chemistry Practiced?
- Guiding Principles of Practicing the Profession
- Confidentiality of Genetic Information
- Confidentiality of Patient Medical Information
- Allocation of Resources
- Codes of Conduct
- Publishing Issues
- Conflicts of Interest
- What is in this Textbook?
- 2. Analytical and Clinical Evaluation of Methods
- Assay Selection Overview
- Basic Statistics
- Technical Validity of Analytical Assays
- Assay Comparison
- Monitoring Serial Results
- Traceability
- Diagnostic Accuracy of Laboratory Tests
- 3. Preanalytical Variation
- Preanalytical Phase
- Endogenous Interference Factors
- Exogenous Interference Factors
- Specific Considerations
- 4. Biological Variation
- Nature of Biological Variation
- Generation of Data on Components of Biological Variation
- Databases
- Interpretation and Use of Data
- Reference Change Values
- Other Applications
- Overall Conclusions
- 5. Establishment and Use of Reference Values
- Establishment of Reference Values
- Use of Reference Values
- 6. Specimen Collection, Processing, and Other Preanalytical Variables
- Patient Identification
- Types of Specimens
- Handling of Specimens for Analysis
- Maintenance of Specimen Identification
- Conclusion
- 7. Quality Management
- Measurement Procedure Performance as a Prerequisite for a Quality Control Plan
- Developing a Quality Control Plan and Implementing Internal Quality Control Procedures
- External Quality Assessment or Proficiency Testing
- 8. Principles of Basic Techniques and Laboratory Safety
- Chemicals
- Reference Materials
- Basic Techniques and Procedures
- Safety
- Part II. Analytical Techniques and Instrumentation
- 9. Optical Techniques
- Photometry and Spectrophotometry
- Instrumentation
- Reflectance Photometry
- Atomic Absorption Spectrophotometry
- Fluorometry
- Luminometry
- Nephelometry and Turbidimetry
- 10. Electrochemistry and Chemical Sensors
- Potentiometry
- Ion-Selective Membrane Electrodes
- Voltammetry and Amperometry
- Conductometry
- Coulometry
- Optical Chemical Sensors
- Biosensors
- 11. Electrophoresis
- Basic Concepts
- Theory of Electrophoresis
- Clinical Electrophoresis
- Capillary Electrophoresis
- Types of Electrophoresis
- Technical Considerations
- Speciality Electrophoresis Techniques
- 12. Chromatography
- Basic Principles of Chromatography
- Gas Chromatography
- Liquid Chromatography
- Planar Chromatography
- 13. Mass Spectrometry
- Basic Concepts
- Instrumentation
- Clinical Applications
- 14. Enzyme and Rate Analyses
- Enzyme Nomenclature
- Enzymes as Proteins
- Specificity and the Active Center
- Isoenzymes and Other Multiple Forms of Enzymes
- Enzymes as Catalysts
- Units for Expressing Enzyme Activity
- Enzyme Kinetics
- Factors Governing the Rate of Enzyme-Catalyzed Reactions
- Analytical Enzymology
- 15. Immunochemical Techniques
- Basic Concepts and Definitions
- Antigen-Antibody Binding
- Qualitative Methods
- Quantitative Methods
- Cell-Based and Tissue-Based Immunochemical Techniques
- 16. Automation
- Nonanalytical Automation for the Clinical Laboratory
- Practical Considerations
- Automation of the Analytical Processes
- Areas of Analytical Automation
- 17. Point-of-Care Testing
- Technology
- Quality Management
- Evidence of Effectiveness
- Part III. Analytes
- 18. Amino Acids, Peptides, and Proteins
- Amino Acids
- Peptides
- Proteins
- Protein Catabolism
- 19. Serum Enzymes
- Basic Concepts
- Muscle Enzymes
- Liver Enzymes
- Pancreatic Enzymes
- Other Clinically Important Enzymes
- 20. Tumor Markers
- Cancer—an Overview
- Historical Background
- General Principles Guiding the Use of all Tumor Markers
- Quality Requirements for Tumor Marker use in the Clinic
- Conclusion
- 21. Kidney Function Tests
- Proteinuria: Total Protein and Albumin
- Creatinine
- Cystatin C
- Urea
- Uric Acid (Urate)
- Assessment of Kidney Function: Glomerular Filtration Rate
- 22. Carbohydrates
- Chemistry of Carbohydrates
- Biochemistry and Physiology
- Analytical Methodology
- 23. Lipids, Lipoproteins, Apolipoproteins, and Other Cardiac Risk Factors
- Basic Lipids
- Lipoproteins
- Apolipoproteins
- Metabolism of Lipoproteins
- Clinical Significance
- Adult Management of Lipoprotein Disorders
- Analysis of Lipids, Lipoproteins, and Apolipoproteins
- Advanced Lipid and Lipoprotein Testing for Coronary Heart Disease Risk Assessment
- 24. Electrolytes and Blood Gases
- Electrolytes
- Specimens for Electrolyte Determination
- Sodium
- Potassium
- Methods for the Determination of Sodium and Potassium
- Electrolyte Exclusion Effect
- Methods for Determination of Chloride in Body Fluids
- Sweat Chloride
- Bicarbonate (Total Carbon Dioxide)
- Principles of Osmotic Pressure and Osmosis
- Blood Gases and pH
- Behavior of Gases
- Application of the Henderson-Hasselbalch Equation in Blood Gas Measurements
- Oxygen in Blood
- Hemoglobin Oxygen Saturation
- Determination of pCO2, pO2, and pH
- Temperature Correction of Measured pH, pCO2, and pO2
- Continuous and Noninvasive Monitoring of Blood Gases
- 25. Hormones
- Background
- Synthesis, Release, and General Action of Hormones
- Cell Signaling Mechanisms of Hormone Receptors
- Signal Transduction From Cell Surface G-Protein–Coupled Receptors
- Signal Transduction From Cell Surface Enzyme–Coupled Receptors
- Clinical Disorders of Hormone Action
- Techniques for Measurement of Hormones and Related Analytes
- 26. Catecholamines and Serotonin
- Chemistry, Biosynthesis, Release, and Metabolism
- Physiology of Catecholamine and Serotonin Systems
- 27. Vitamins and Trace Elements
- Vitamin and Trace Element Status
- Vitamins
- Trace Elements
- Laboratory Assessment of Trace Element Status
- Analytical Methods for Measuring Trace Elements
- 28. Hemoglobin, Iron, and Bilirubin
- Hemoglobin
- Iron
- Bilirubin
- 29. Porphyrins and Porphyrias
- Porphyrin Chemistry
- Heme Biosynthesis
- Regulation of Heme Biosynthesis
- Solubility and Excretion of Heme Precursors
- The Porphyrias
- Laboratory Diagnosis of Porphyria
- Laboratory Analysis
- 30. Therapeutic Drug Monitoring
- Fundamental Principles of Applied Pharmacokinetics
- Therapeutic Drug Monitoring of Specific Drugs in Common Clinical Use
- 31. Clinical Toxicology
- Screening Procedures for Detection of Drugs
- Pharmacology and Analysis of Specific Drugs and Toxic Agents
- Agents that Cause Cellular Hypoxia
- Alcohols of Toxicologic Interest
- Analgesics (Nonprescription)
- Agents Related to Anticholinergic Toxidrome
- Antihistamines
- Agents Related to Cholinergic Toxidrome
- Drugs of Abuse
- Drug-Facilitated Crimes
- Pain Management
- Detection of Drugs of Abuse using other Types of Specimens
- 32. Toxic Elements
- Background
- Content
- Prevalence of Elemental Toxicities
- Diagnosing Toxicity
- Treatment
- Specific Elements
- Part IV. Pathophysiology
- 33. Diabetes Mellitus
- Classification
- Categories of Increased Risk for Diabetes
- Hormones that Regulate Blood Glucose Concentration
- Measurement of Insulin, Proinsulin, C-Peptide, and Glucagon
- Pathogenesis of Type 1 Diabetes Mellitus
- Pathogenesis of Type 2 Diabetes Mellitus
- Diagnosis of Diabetes
- Diagnosis of Gestational Diabetes Mellitus
- Chronic Complications of Diabetes Mellitus
- Role of the Clinical Laboratory in Diabetes Mellitus
- Self-Monitoring of Blood Glucose
- Alternatives to Meters for Monitoring of Blood Glucose
- Ketone Bodies
- Glycated Proteins
- Albuminuria
- 34. Cardiovascular Disease
- Anatomy and Physiology of the Heart
- Cardiac Biomarkers
- 35. Kidney Disease
- Anatomy
- Kidney Function
- Kidney Physiology
- Pathophysiology of Kidney Disease
- Diseases of the Kidney
- Renal Replacement Therapy
- 36. Physiology and Disorders of Water, Electrolyte, and Acid-Base Metabolism
- Water and Electrolytes—Composition of Body Fluids
- Extracellular and Intracellular Compartments
- Electrolytes
- Sodium
- Potassium
- Chloride
- Acid-Base Physiology
- Acid-Base Balance and Acid-Base Status
- Buffer Systems and their Role in Regulating the PH of Body Fluids
- Respiratory Mechanism in the Regulation of Acid-Base Balance
- Renal Mechanisms in the Regulation of Acid-Base Balance
- Conditions Associated With Abnormal Acid-Base Status and Abnormal Electrolyte Composition of Blood
- Metabolic Alkalosis (Primary Bicarbonate Excess)
- Respiratory Acidosis
- Respiratory Alkalosis
- 37. Liver Disease
- Anatomy of the Liver
- Biochemical Functions of the Liver
- Diseases of the Liver
- Liver Transplantation
- 38. Gastric, Pancreatic, and Intestinal Function
- Introduction to the Anatomy and Physiology of the Gastrointestinal Tract
- Stomach: Diseases and Laboratory Investigations
- Intestinal Disorders and their Laboratory Investigation
- Pancreas: Disease and Assessment of Exocrine Pancreatic Function
- 39. Bone and Mineral Metabolism
- Overview of Skeletal Metabolism
- Biochemical Measurements in Metabolic Bone Disease
- Phosphate
- Magnesium
- Hormones Regulating Bone and Mineral Metabolism
- Biochemical Markers of Bone Turnover
- Metabolic Bone Diseases
- 40. Disorders of the Pituitary Gland
- Background
- Content
- Anatomy
- Hypothalamic Regulation
- Summary of Pituitary-Related Disorders
- 41. Adrenal Cortex
- Anatomy
- Physiology and Regulation of Adrenocortical Hormones
- Adrenocortical Hormones in the Circulation
- Disorders of the Adrenal Cortex
- Laboratory Evaluation of Adrenocortical Function
- 42. Thyroid Disorders
- Anatomy
- Thyroid Hormones
- Iodine and Thyroid Disorders
- Extrathyroidal Factors that Affect Thyroid Function
- Evaluation of Thyroid Function
- Other Methods
- Thyroid Disease
- Thyroid Disorders in Pregnancy and Postpartum
- Graves Disease in Pregnancy
- Thyroid Neoplasia
- Thyroid Disease in Children
- Genetics
- 43. Reproduction-Related Disorders
- Background
- Male Reproductive Biology
- Hypothalamic-Pituitary-Gonadal Axis
- Female Reproductive Biology
- Infertility
- Analytic Methods for Reproductive Hormones
- 44. Pregnancy and Prenatal Testing
- Human Pregnancy
- Maternal Adaptation
- Functional Development of The Fetus
- Maternal and Fetal Health Assessment
- Complications of Pregnancy
- Prenatal Screening for Fetal Defects
- Prenatal Screening Tests
- Analytical Methodology
- 45. Newborn Screening and Inborn Errors of Metabolism
- Inheritance Pattern of Metabolic Disorders
- Newborn Screening
- Inborn Errors of Metabolism
- Diagnostic Tests for Inherited Disorders of Metabolism
- 46. Pharmacogenetics
- Principles of Pharmacogenetics
- Specific Examples of Pharmacogene Associations
- Future Directions
- Part V. Molecular Diagnostics
- 47. Molecular Principles
- Molecular Biology Essentials
- Nucleic Acid Structure and Function
- Central Dogma of Molecular Biology
- Epigenetics
- The Human Reference Genome
- Projects Inspired by the Human Genome Project
- Nonhuman Genomes
- Variation in the Human Genome
- Naming of Chromosomes, Genes, and Variants
- Variant Databases
- Bioinformatics
- Cancer Genomics
- Massively Parallel Sequencing of Solid Tumor Samples
- Trends in Cancer Genomics
- Summary
- 48. Molecular Techniques
- Nucleic Acid Preparation
- Amplification Techniques
- Detection Techniques
- Discrimination Techniques
- 49. Molecular Applications
- Infectious Disease
- Sexually Transmitted Infections
- Respiratory Tract Infections
- Human Microbiome and Metagenomics
- Genetics
- Massively Parallel Sequencing
- Whole-Exome Sequencing
- Genetics of Hematopoietic Malignancies
- Numerical Chromosomal Abnormalities
- Recurrent Translocations
- Gene Mutations in Hematopoietic Malignancies
- Circulating Tumor Cells and Circulating Tumor CELL FREE DNA
- Circulating Tumor CELL FREE DNA
- Circulating Nucleic Acids for Prenatal Diagnostics
- Biology of Circulating Cell-Free Fetal Nucleic Acids in Maternal Plasma
- Analytical Aspects
- Appendix. Reference Information for the Clinical Laboratory
- Index
- IUPAC Periodic Table of the Elements
- Language: English
- Edition: 8
- Published: October 31, 2018
- Imprint: Saunders
- eBook ISBN: 9780323549738
NR
Nader Rifai
Affiliations and expertise
Professor Department of Pathology Harvard Medical School; The Louis Joseph Gay-Lussac Chair in Laboratory Medicine Boston Children's Hospital; Director of Clinical Chemistry Laboratory Medicine Boston Children's Hospital Boston, Massachusetts