The Use of Radioautography in Investigating Protein Synthesis
- 1st Edition - February 16, 2016
- Editors: C. P. Leblond, Katherine Brehme Warren
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 5 4 0 5 - 0
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 8 2 0 2 - 2
The Use of Radioautography in Investigating Protein Synthesis is a collection of papers that discusses the tracing of labeled proteins through photographic emulsion, particularly… Read more
The Use of Radioautography in Investigating Protein Synthesis is a collection of papers that discusses the tracing of labeled proteins through photographic emulsion, particularly radioautography or autoradiography. The papers discuss proteins at the site of their synthesis, their subsequent migration through the cell, their secretions, and the formation of the basement lamella in salamanders. One paper outlines the primary aims in specimen preparation for electron microscope autoradiographic techniques and the procedures to achieve them. Another paper reports on migrating proteins (CNP) and nonmigrating proteins of the Amoeba proteus nucleus in which nonmigrating proteins are sedimentable by centrifugation in situ in the nucleus. One paper investigates the extent of protein synthesis for cell renewal in thoracic duct lymphocytes (TDL) or that for export or intracellular turnover. The protein synthesized by the large cell population in TDL is associated with mitosis and cell renewal. A major portion of that synthesized by the medium cells (80 per cent) and practically all of that produced by small cells are for purposes other than cell renewal. The collection can prove beneficial to biochemists, micro-biologists, bio-physicists, cytologists, histologists, pathologists, cellular researchers, and academicians involved in the study of cellular biology.
List of Contributors
Preface
Opening Address
Techniques and Methods
State of Light Autoradiography
Assessment of Technical Steps in Electron Microscope Autoradiography
Comparison of Emulsions and Techniques in Electron Microscope Radioautography
Application of Freeze-Drying and Formaldehyde-Vapor Fixation to Radioautographic Localization of Soluble Amino Acids
Protein Synthesis in Nucleus
Interchange of Protein Between Nucleus and Cytoplasm
Amino Acid Deprivation and Deoxyribonucleic Acid Synthesis in Tetrahymena
Cytoplasmic Protein Synthesis in Cells of Various Types and Its Relation to Nuclear Protein Synthesis
Protein Synthesis in Cells of Various Types
Protein Synthesis in Lymphocytes
Fate of Newly Synthesized Proteins in Neurons
Protein Synthesis in the Ear Under Normal and Pathological Conditions
Amino Acid Utilization in the Synthesis of Enamel and Dentin Matrices as Visualized by Autoradiography
Protein Synthesis and Cells of the Skeletal System
Synthesis and Turnover of Collagen in Periodontal Tissues
Secretory Processes
Secretion of Protein Enzymes by the Acinar Cells of the Rat Pancreas
Synthesis and Secretion of Collagen by Fibroblasts in Healing Wounds
The Formation of the Basement Lamella in Regenerating Salamander Limbs
The Elaboration of Thyroglobulin by the Thyroid Follicular Cells
What Radioautography has Added to Protein Lore
Author Index
- Edition: 1
- Published: February 16, 2016
- Language: English