The Proteins Pt 3

List of Contributors

Preface

Contents of Other Volumes

Chapter 1. Strategy and Methods of Sequence Analysis

I. Introduction

II. Prerequisite Considerations

III. Prerequisite Analytical Characterizations

IV. Prerequisite Preparative Treatments

V. Cleavage of the Polypeptide Chain

VI. Peptide Fractionation

VII. Sequence Analysis of Isolated Peptides

VIII. Overlapping Peptides: Reconstruction of the Primary Structure

IX. Assignment of Disulfide Bridges

X. Possible Sources of Error in Sequence Analysis

XI. Applications of Protein Sequence Data

XII. Anticipated Developments in the Primary Structural Analysis of Proteins

XIII. Documentation of Sequence Data for Publication: Concluding Remarks

References

Chapter 2. Automated Methods for Sequence Analysis

I. Introduction

II. Automated Edman Degradation: The Edman Reaction

III. Use of Alternate Degradation Reagents

IV. Instrumental Design: The Sequenator

V. Identification Procedures

VI. Operation of Sequenator

VII. Special Problems

VIII. Problems in Sequence Identification

IX. Automated Degradation of Short Peptides

X. High-Sensitivity Automated Sequence Analysis

XI. Applications of the Sequenator

XII. Strategy of Sequence Determination with the Sequenator

XIII. Automated Solid-Phase Sequence Analysis

XIV. Automated Sequence Analysis by Mass Spectrometry

XV. Future Prospects for Automated Sequence Analysis

References

Chapter 3. The Role of Sulfur in Proteins

I. Introduction

II. The Chemistry and Reactions of Sulfhydryl, Disulfide, and Thiolester Groups in Proteins

III. Analytical Methods

IV. The Role of Sulfhydryl, Disulfide, and Thioether Groups in Proteins

References

Chapter 4. X-Ray Structure of Proteins

I. Introduction

II. X-Ray Crystallography

III. Protein Crystals

IV. Protein Structures

V. Conclusions

References

Author Index

Subject Index