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The Proteins Pt 3
- 3rd Edition - January 1, 1977
- Editor: Hans Neurath
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 3 9 4 2 1 1 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 6 2 0 6 - 7
The Proteins, Volume III, Third Edition deals with the structure of proteins in terms of composition, amino acid sequence, and three-dimensional conformation. Special consideration… Read more
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Request a sales quoteThe Proteins, Volume III, Third Edition deals with the structure of proteins in terms of composition, amino acid sequence, and three-dimensional conformation. Special consideration is given to sulfur and the sulfur-containing amino acids because of their unique contribution to the structure and reactivity of certain proteins. The book reviews the strategies and procedures for determining the amino acid sequence of a protein. The "spinning-cup" sequenator method of Edman and Begg; the role of sulfur in proteins; and the three-dimensional structure of some crystalline proteins by X-ray diffraction analysis are covered as well. Protein chemists, medical and biological researchers, and students who require more knowledge of protein chemistry will find the book extremely useful.
List of Contributors
Preface
Contents of Other Volumes
Chapter 1. Strategy and Methods of Sequence Analysis
I. Introduction
II. Prerequisite Considerations
III. Prerequisite Analytical Characterizations
IV. Prerequisite Preparative Treatments
V. Cleavage of the Polypeptide Chain
VI. Peptide Fractionation
VII. Sequence Analysis of Isolated Peptides
VIII. Overlapping Peptides: Reconstruction of the Primary Structure
IX. Assignment of Disulfide Bridges
X. Possible Sources of Error in Sequence Analysis
XI. Applications of Protein Sequence Data
XII. Anticipated Developments in the Primary Structural Analysis of Proteins
XIII. Documentation of Sequence Data for Publication: Concluding Remarks
References
Chapter 2. Automated Methods for Sequence Analysis
I. Introduction
II. Automated Edman Degradation: The Edman Reaction
III. Use of Alternate Degradation Reagents
IV. Instrumental Design: The Sequenator
V. Identification Procedures
VI. Operation of Sequenator
VII. Special Problems
VIII. Problems in Sequence Identification
IX. Automated Degradation of Short Peptides
X. High-Sensitivity Automated Sequence Analysis
XI. Applications of the Sequenator
XII. Strategy of Sequence Determination with the Sequenator
XIII. Automated Solid-Phase Sequence Analysis
XIV. Automated Sequence Analysis by Mass Spectrometry
XV. Future Prospects for Automated Sequence Analysis
References
Chapter 3. The Role of Sulfur in Proteins
I. Introduction
II. The Chemistry and Reactions of Sulfhydryl, Disulfide, and Thiolester Groups in Proteins
III. Analytical Methods
IV. The Role of Sulfhydryl, Disulfide, and Thioether Groups in Proteins
References
Chapter 4. X-Ray Structure of Proteins
I. Introduction
II. X-Ray Crystallography
III. Protein Crystals
IV. Protein Structures
V. Conclusions
References
Author Index
Subject Index
- No. of pages: 678
- Language: English
- Edition: 3
- Published: January 1, 1977
- Imprint: Academic Press
- Paperback ISBN: 9780123942111
- eBook ISBN: 9780323162067
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