The Proteins Pt 1

List of Contributors

Preface

Chapter 1. Molecular Sieve Methods of Analysis

I. General Introduction

II. Chromatographic Transport Methods

III. Equilibrium Partitioning Methods

IV. Determination of Molecular Size and Weight

V. Molecular Sieve Properties of Polydisperse Solutes

VI. Self-Association of Protein Subunits

VII. Association between Dissimilar Subunits

VIII. Binding of Small Molecules to Proteins

References

Chapter 2. Biospecific Affinity Chromatography and Related Methods

I. Introduction

II. Basic Principles

III. Nomenclature

IV. Matrices (Carriers)

V. Methods for Attachment of Ligands

VI. Adsorption-Desorption Procedures

VII. Applications

VIII. Concluding Remarks

References

Chapter 3. Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

I. Introduction

II. Interaction of Proteins and SDS

III. Molecular Weight Determinations on SDS Gels

IV. Methods for Further Characterization of Proteins Separated on SDS Gels

References

Chapter 4. Sedimentation Analysis of Proteins

I. Introduction: The Role of Analytical Sedimentation Methods in Protein Chemistry

II. Moving-Boundary Sedimentation Transport

III. Zonal Sedimentation Transport in Density Gradients

IV. Sedimentation Equilibrium

V. Advances and Trends in Ultracentrifuge Technology

References

Chapter 5. Quaternary Structure of Proteins

I. Introduction

II. Stoichiometric Constitution

III. Geometric Arrangements of Identical Subunits

IV. Energetics of Self-Assembly

V. Intersubunit Communication

VI. Functional Aspects

VII. Conclusion

References

Chapter 6. Electron Microscopy of Proteins

I. Introduction

II. Relationship between the Electron Microscope Image and the Specimen

III. Specimens

IV. Interpretation and Analysis of Images

V. Results

VI. Recent Developments and Possible Future Needs

References

Author Index

Subject Index