The Peptides Analysis, Synthesis, Biology
Modern Techniques of Conformational Structural, and Configurational Analysis
- 1st Edition - November 10, 2012
- Editor: Erhard Gross
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 3 3 2 5 6 - 0
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 5 5 6 3 - 2
The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides… Read more
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Request a sales quoteThe Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides comprehensive and critical reviews of important developments in all areas of peptide research including analysis, synthesis, and biology. X-ray structure studies, amino acid analysis, and chiroptical analysis of configuration are discussed, along with solid-phase sequencing and ultramicroanalysis with the aid of fluorescence. This volume is comprised of six chapters and begins with an account of crystal structure analysis on molecules containing 2-12 peptide units, focusing on the variety of intramolecular hydrogen bonds, cis peptide units, and multiple conformation. Conformational changes upon complexation with metal ions are considered, together with the inclusion of solvents as integral parts of a molecular structure. The following chapters explore the conformations of insulin, glucagon, pancreatic polypeptide and related molecules, as well as the molecular biology of these hormones based on crystal structures; the usefulness of chiroptical techniques for determining the absolute configuration of amino acids and small peptides; and ultramicroanalysis of peptides and proteins by high performance liquid chromatography and fluorescence detection. The final chapter looks at the status and future potential of solid-phase sequencing. This book is intended as a reference for specialists, a guide for the novice, and a forum for investigators concerned with research on peptides and proteins.
List of Contributors
Preface
Nomenclature and Abbreviations
Contents of Previous Volumes
Chapter 1. X-Ray Analysis: Conformation of Peptides in the Crystalline State
I. Introductory Remarks
II. Cyclic Dipeptides
III. Peptides Containing Tri-, Tetra-, and Pentapeptide Rings
IV. Hexapeptide and Heptapeptide Rings
V. Cyclic Octapeptides to Pentadecapeptides
VI. Linear Peptides
VII. Geometry of the Peptide Unit
VIII. Intramolecular Hydrogen Bonds
IX. Summary
References
Chapter 2. Crystal Structure Analysis of the Larger Peptide Hormones
I. Introduction
II. Insulin and Proinsulin
III. Hormones Homologous to Insulin
IV. Glucagon
V. Pancreatic Polypeptide
VI. Placental and Pituitary Hormones
VII. Conclusion and Outlook
References
Chapter 3. Determination of the Absolute Configuration of α-Amino Acids and Small Peptides by Chiroptical Means
I. Introduction
II. Definitions: Optical Rotatory Dispersion and Circular Dichroism
III. Chiroptical Properties of Amino Acids
IV. Chiroptical Properties of Metal Complexes and Chromophoric Derivatives of α-Amino Acids
V. Chiroptical Properties of Oligopeptides
VI. Chiroptical Properties of Metal Complexes and Chromophoric Derivatives of Di- and Oligopeptides
VII. Solvent, Salt, and Temperature Effects
VIII. Instrumentation
IX. Calibration and Standards
X. Conclusion and Outlook
References
Chapter 4. Ultramicroanalysis of Peptides and Proteins by High-Performance Liquid Chromatography and Fluorescence Detection
I. Introduction
II. Detection
III. High-Performance Liquid Chromatography
IV. Applications
V. Conclusion
References
Chapter 5. Amino Acid Analysis of Peptides
I. Introduction
II. Historical
III. Experimental
IV. Concluding Remarks
References
Chapter 6. Solid-Phase Sequencing of Peptides and Proteins
I. Introduction
II. Chemistry of the Edman Degradation
III. Immobilization of Peptides
IV. Supports
V. Instrumentation
VI. Sequencing Strategies
VII. Scope and Limitations
References
Author Index
Subject Index
- No. of pages: 330
- Language: English
- Edition: 1
- Published: November 10, 2012
- Imprint: Academic Press
- Paperback ISBN: 9780124332560
- eBook ISBN: 9780323155632
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