The Intestine
- 1st Edition - June 15, 2021
- Imprint: Academic Press
- Editors: Ahmed El-Hashash, Eiman Abdel Meguid
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 2 6 9 - 1
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 1 2 7 1 - 4
The intestine is among the leading organs, in which several cutting edge in vitro and in vivo research tools and approaches have recently been developed and used to investigate st… Read more
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Request a sales quoteThe intestine is among the leading organs, in which several cutting edge in vitro and in vivo research tools and approaches have recently been developed and used to investigate stem cell biology/function, and the potential applications of stem cells in the treatment of intestinal diseases. These cutting-edge research tools and approaches involve human and murine organoid cultures, genetic editing in vitro and in vivo, human induced pluripotent cell (iPS cell) models of disease, haploid cells for genetic as well as compound screening paradigms, genetically engineered mice, and stem cell transplantation to cure diseases.
Stem Cell Innovation in Health and Disease: Volume 1: The Intestine contains two major sections describing cutting edge research for understanding stem cell functions in the intestine, and for developing methods to bring stem cells from bench to bedside; respectively. Each section includes insights ranging from using mouse and human organoid cultures, genetic editing in vitro and in vivo, and human induced pluripotent cells (iPSCs) to study stem cell functions and model intestinal diseases, through the cutting-edge research, including the potential application of iPSCs, ESCs and blood stem cells (stem cell transplants) in the treatment of intestinal diseases/disorders. This volume, therefore, discusses the fact-based promise of stem cells and regenerative medicine in the intestine in the real world.
- Provides intensive scientific background and most recent information on cutting edge research to understand intestinal stem cell functions and develop methods to bring stem cells from bench to bedside for different intestinal diseases
- Analyzes the current state, opportunities, and challenges of innovative technologies and stem cells from bench to bed, including organoids and the CRISPR gene editing system in the intestine
- Contains two major sections describing cutting-edge research for understanding stem cell functions and for developing methods specific to the intestine
Researchers and practitioners in the following fields: Life Science: Stem Cell Biology Research and Biotechnology; Medicine: Stem Cell Research, Regenerative Medicine; Healthcare: Disease, Medical Education, Stem Cells, Regenerative Medicine; Engineering: Biomedical Engineering; Graduate and undergraduate students in these fields
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Epigraph
- List of contributors
- About the editors
- Foreword 1
- Foreword 2
- Preface
- Acknowledgments
- Chapter 1. Cutting-edge tools and approaches for stem cell research and application in intestinal diseases
- Abstract
- 1.1 Introduction
- References
- Chapter 2. Organotypic intestinal cell culture as a new modality for intestinal function and cellular processes
- Abstract
- 2.1 Defining organotypic intestinal cell culture models
- 2.2 Intestinal structure and functions
- 2.3 Modeling of intestinal functions using organotypic cell culture models
- 2.4 Toward emulation of organ-level functions
- Acknowledgment
- References
- Chapter 3. Role of human gastrointestinal organoids in discovery and translational medicine
- Abstract
- 3.1 Introduction
- 3.2 Current application and perspective use of organoids in precision and personalized medicine
- 3.3 Ethical perspective on organoids biobanks
- References
- Chapter 4. Engineered stem cells combine stem cell and gene therapy approaches to move intestine therapy from bench to bed
- Abstract
- 4.1 Introduction
- 4.2 Genome editing
- 4.3 Genome editing of stem cells
- 4.4 Therapeutic applications of gene-edited stem cells
- 4.5 Gene-edited stem cell therapy for intestinal diseases
- 4.6 Conclusion and future prospects
- References
- Chapter 5. Role of CRISPR/Cas9 and other gene editing/engineering technology in intestine diseases
- Abstract
- 5.1 Brief introduction of CRISPR-Cas9
- 5.2 CRISPR/Cas 9 application in colorectal cancers
- 5.3 CRISPR/Cas9 applications in inflammatory bowel disease
- 5.4 CRISPR/Cas9 applications in gut microbiota
- 5.5 Other gene editing tools
- 5.6 Limitations and perspectives
- References
- Chapter 6. Application of new approaches for intestinal repair and regeneration via stem cell–based tissue engineering
- Abstract
- 6.1 Structure and cellular components of the small intestine
- 6.2 Stem cells used in small intestine regeneration
- 6.3 Developing biological tissue-engineered grafts
- 6.4 Tissue-engineered small intestine
- 6.5 Role of stem cell–based transplantation in intestinal regeneration
- 6.6 Conclusion and future directions
- References
- Chapter 7. Induced pluripotent stem cells in intestinal diseases
- Abstract
- 7.1 Introduction
- 7.2 iPSC-based disease modeling
- 7.3 Intestinal organoids
- 7.4 iPSCs and organoids in intestinal diseases
- 7.5 Clinical trials
- 7.6 Limitations
- References
- Chapter 8. Potential of embryonic stem cells for treating intestinal diseases
- Abstract
- 8.1 Embryonic stem cells
- 8.2 ESC-based therapy of the gastrointestinal diseases
- 8.3 Conclusions
- References
- Chapter 9. Stem-cell therapy with bone marrow (hematopoietic) stem cells for intestinal diseases
- Abstract
- 9.1 Introduction
- 9.2 Bone-marrow HSC niche
- 9.3 Clinical applications of HSCs
- 9.4 Autologous hematopoietic stem cell transplantation for autoimmune diseases
- 9.5 HSCs for the treatment of genetic blood cell diseases
- 9.6 HSCs for the treatment of intestinal diseases
- 9.7 Conclusion, challenges, and future directions
- References
- Chapter 10. Role of mesenchymal and other stem cell therapy in intestinal diseases
- Abstract
- 10.1 Introduction
- 10.2 Mesenchymal stem cells
- 10.3 Hematopoietic stem cells
- 10.4 Intestinal stem cells
- 10.5 Conclusions and prospects
- References
- Chapter 11. General discussion, conclusion remarks, and future directions
- Abstract
- 11.1 Advances of organotypic intestinal cell culture and gene editing/engineering in intestinal repair, regeneration, and diseases: current challenges and future prospectives
- References
- Index
- Edition: 1
- Published: June 15, 2021
- No. of pages (Paperback): 204
- No. of pages (eBook): 204
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780128212691
- eBook ISBN: 9780128212714
AE
Ahmed El-Hashash
Dr. Ahmed Hashash is currently Endowed Chair and Distinguished Professor at Elizabeth City State University, campus of the University of North Carolina (UNC). He received his PhD from Manchester University, UK. Dr. Hashash was a fellow of the California Institute of Regenerative Medicine (CIRM) and New York University Medical School (MSSM), USA. He was Assistant Professor and Principal Investigator of Stem Cell & Regenerative Medicine at School of Medicine University of California, USA. Dr Hashash was Associate Professor of Biomedicine at the University of Edinburgh (UK) Zhejiang Intl. campus and Texas A &M University, USA from 2017 to 2025. He has published more than 40 papers in reputed international journals, 10 books with Elsevier and Springer and over 20 book chapters, and is serving as an editorial board member of repute.
Affiliations and expertise
Endowed Chair, Distinguished Professor, University of North CarolinaEM
Eiman Abdel Meguid
She completed her PhD thesis at Eberhard-Karls Universität Tübingen, Germany. Dr Abdel Meguid is an innovative educator and researcher and has published multiple scientific works in leading journals and is a reviewer and a member of the editorial board of a number of journals. Dr Abdel Meguid taught gross anatomy to medical, dental, human biology students and students enrolled in MSc in clinical anatomy. Her research interests are in the areas of stem cells, lung development, anatomical pedagogy, teaching strategies, and the integration of novel technologies to enhance learning.
Affiliations and expertise
Senior Lecturer, Centre for Biomedical Sciences Education, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, UKRead The Intestine on ScienceDirect