The Immunological Synapse - Part B
- 1st Edition, Volume 178 - July 11, 2023
- Imprint: Academic Press
- Editors: Clément Thomas, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 9 1 6 3 - 3
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 9 1 6 4 - 0
The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first vo… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteThe Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay.
- Covers various methods related to the study of the immunological synapse
- Includes detailed, point-by-point, methods as well as various important notes
- Provides the authority and expertise from an international board of leading scientists
Anyone conducting research involving immunological synapses from beginner to expert level, and from technicians to graduate students, postdoctoral researchers, and principal investigators
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Chapter 1: Live-cell imaging for analysis of the NK cell immunological synapse
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- Acknowledgments
- References
- Chapter 2: Analyzing the activity of the proteases granzyme B and caspase-8 inside living cells using fluorescence localization reporters
- Abstract
- 1: Introduction
- 2: Material and methods
- 3: Concluding remarks
- 4: Notes
- Acknowledgments
- Declaration of interests
- References
- Chapter 3: T cell activation and effector function in the human Jurkat T cell model
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Discussion
- 5: Notes
- Acknowledgments
- References
- Chapter 4: A tractable microscopy- and flow cytometry-based method to measure natural killer cell-mediated killing and infiltration of tumor spheroids
- Abstract
- 1: Introduction
- 2: Protocol
- 3: Notes
- 4: Concluding remarks
- Acknowledgments
- References
- Chapter 5: Kinetic, imaging based assay to measure NK cell cytotoxicity against adherent cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- Declaration of interests
- References
- Chapter 6: Correlative light and electron microscopy to explore the lytic immunological synapse between natural killer cells and cancer cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- References
- Chapter 7: Metrics of 2D immunological synapses in human T cells via high-content confocal cell imaging
- Abstract
- 1: Introduction
- 2: Material
- 3: Procedure
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- References
- Chapter 8: Kinetic measurements of human CD8+ T cell cytotoxic activity in a 384-well plate format
- Abstract
- 1: Introduction
- 2: Material
- 3: Procedure
- Acknowledgments
- References
- Chapter 9: Imaging PIP2 and BCR microclusters in B cell immunological synapse
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- Notes
- Acknowledgments
- Disclosure
- References
- Chapter 10: Activation kinetics of regulatory molecules during immunological synapse in T cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- Acknowledgments
- Discussion
- References
- Chapter 11: Immunological synapse-driven transfer of extracellular vesicle microRNAs in primary lymphocytes
- Abstract
- 1: Introduction
- 2: Material
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- References
- Chapter 12: Generation of tumor spheroids in microwells to study NK cell cytotoxicity, infiltration and phenotype
- Abstract
- 1: Introduction
- 2: Materials
- 3: Protocol
- 4: Notes
- References
- Edition: 1
- Volume: 178
- Published: July 11, 2023
- No. of pages (Hardback): 222
- No. of pages (eBook): 318
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780443191633
- eBook ISBN: 9780443191640
CT
Clément Thomas
Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion.
Affiliations and expertise
Group Leader, Cytoskeleton and Cancer Progression, Department of Cancer Research, Luxembourg Institute of Health, LuxembourgLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead The Immunological Synapse - Part B on ScienceDirect