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Elsevier

The Immunoassay Handbook

Immunoassays are biochemical tests that rely on antibodies (a special type of protein) to bind to specific molecular structures (antigens). They have become the most co… Read more

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Description

Immunoassays are biochemical tests that rely on antibodies (a special type of protein) to bind to specific molecular structures (antigens). They have become the most commercially successful technology in diagnostics, from home pregnancy testing kits to AIDS testing. Highly illustrated and clearly written, The Immunoassay Handbook provides an excellent guide to this fast-growing industry.

Revised and updated, this third edition offers a balanced review of practical information and clinical applications in the field of immunoassay technology. Comprehensive and expertly written, The Immunoassay Handbook will be invaluable for a wide range of users from researchers developing immunoassay systems to developmental and marketing staff who need an excellent overview of how immunoassay principles, systems and applications work.

With 68 chapters covering a wide range of topics, and new chapters looking at: surface plasmon resonance in kinetic, concentration and binding site analyses, and the foundations of immunochemistry, this handbook is a must read for all laboratory researchers and academics who use immunoassay as a tool including: pathologists, clinical and veterinary chemists, biochemists, students and researchers in immunology, biochemistry and medicine.

Key features

  • This third edition contains updated and new information on advanced technology, including micro- and nanoscale immunoassays
  • Provides a unique mix of practical information coupled with a review of clinical applications, and practical examples
  • Include contributions from world experts including Robert Ritchie and Professor Colin Self

Readership

Laboratory researchers and academics who use immunossay as a tool including pathologists, clinical and veterinary chemists, biochemists, students and researchers in immunology, biochemistry and medicine

Table of contents

Part 1 Principles
Introduction to immunoassay principles (C. Davies).
Non-competitive immunoassays for small molecules - the anti-complex, selective antibody and apposition systems (C.H. Self et al.).
Ambient analyte assay (R. Ekins).
Free analyte immunoassay (N.D. Christofides).
The foundations of immunochemistry NEW (R.F. Ritchie).
Concepts (C. Davies).
Immunoassay development in the in vitro diagnostic industry (D. Brandt, S. Figard).
Antibodies (E. Liddell).
Standardization and calibration (D. Wild).
Separation Systems (D. Wild, W. Kusnezow).
Signal Generation and Detection Systems (Excluding Homogeneous Assays) (L.J. Kricka, D. Wild).
Homogeneous immunoassays (E.F. Ullman).
Calibration curve-fitting (B. Nix, D. Wild).
Conjugation methods (A.H. Dent).
Immunological biosensors (J.K. Gimzewski et al.).
Surface plasmon resonance in kinetic, concentration and binding site analyses (R. Karlsson et al.).
Lab-on-a-chip, micro-and nanoscale immunoassay systems (L.J. Kricka, D. Wild).

Part 2 Product Technology
Introduction to product technology in clinical diagnostic testing (D. Wild).
Market trends (D. Huckle, D. Wild).
Choosing an automated immunoassay system (L.J. Sokoll, D.W. Chan).
Automated system features (A. Truchaud et al.).
Over-the-counter pregnancy test kits (M.J. Wheeler).
Fluorescence microscopy: Microtrak (T. Houts).
Automated batch analyzers: IMx (K. Ford).
Automated bulk reagent random-access analyzers: UniCAP 100 (G. Evans, M. Rilvën).
Automated panel analyzers: PRISM (D. Shah, J. Stewart).
Unitized reagent random-access analyzers: IMMULITEReg and IMMULITE 1000 (A.L. Babson).
Bulk reagent random-access analyzers: ACS:180Reg SE (E. Gramignano).
Bulk reagent random-access analyzers: AxSYMReg (T. Donahoe).
Bulk reagent random access analyzers: ElecsysReg Immunoassay Systems (T. Atkinson).
Bulk reagent random-access analyzers: VitrosReg ECi (D. Wild).
Bulk reagent random-access analyzers: IMMULITEReg2000 and IMMULITE 2500 (A.L. Babson).
Bulk reagent random-access analyzers: ADVIA CentaurReg (L. Taylor, E. Gramignano).
Bulk reagent random-access analyzers: ARCHITECTReg i2000Reg and i2000SRReg analyzers (F.A. Quinn).
CEDIATM, a homogeneous enzyme immunoassay system
(W.A. Coty, R. Loor).
Clinical Chemistry Analyzers: VITROSTMTM Immuno-Rate and MicroTipTM Assays (S.J. Danielson, D.A. Hilborn).
Near-patient tests: the TriageReg system (K.F. Buechler).
Near-patient tests: Stratusâ CS acute careTM
diagnostic system (W.N. McLellan).
Over-the-counter tests: Clearblue pregnancy testä, Clearblue ovulation testä and Clearviewä
(K. May).
Over-the-counter tests: Persona (K. May).

Part 3 Laboratory Management
Subject preparation, sample collection and handling
(C. Wilde).
Laboratory quality assurance (P. Blockx, M. Martin).
Point-of-care testing (J.H. Nichols).
Troubleshooting guide (D. Wild).

Part 4 Applications
Thyroid (R. John et al.).
The Adrenal Cortex (S. Medbak).
Bone metabolism (K.W. Colston, J.C. Stevenson).
Infertility (M.J. Wheeler).
In vitro fertilization and embryo transfer (IVF-ET) (A.C. Olivar).
Hirsutism and virilization in the female (M.J. Wheeler).
Pregnancy (T. Chard).
Growth and growth hormone deficiency (M. Preece, J. Pringle).
Diabetes mellitus (P. Clark).
Hematology (D. Dawson et al.).
Cardiac markers (A.H.B. Wu).
Cancer markers (M.R. Suresh).
Allergy (L. Yman).
Autoimmune diseases (D.F. Keren M.D.).
Sexually transmitted diseases (B.J. Dille et al.).
Congenital diseases of microbiological origin (B.J. Dille et al.).
Hepatitis (I.K. Mushahwar).
Human retroviruses (G.J. Dawson, I.K. Mushahwar).
Dengue virus infections (B.J. Dille, I.K. Mushahwar).
Miscellaneous diseases of microbiological origin (B.J. Dille, I.K. Mushahwar).
Therapeutic Drug Monitoring (TDM) (P.A. Routledge, A.D. Hutchings).
Drugs of abuse (B. Widdop).
Assays for drug-screening applications and research (J.K. Horton et al.).
Immunoassay applications in veterinary diagnostics (E. Workman).

Review quotes

"...it must be stated that there is much of value in the volume [2nd Ed.]. It is a useful and informative addition to any scientific library."—ROBERT F. RITCHIE, FOUNDATION FOR BLOOD RESEARCH, SCARBOROUGH, USA

Product details

About the editor

DW

David Wild

David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.
Affiliations and expertise
Healthcare Industry Consultant, previously at Amersham International, Eastman Kodak, Johnson & Johnson and Bristol-Myers Squibb