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The Chlamydomonas Sourcebook

Volume 1: Introduction to Chlamydomonas and Its Laboratory Use

  • 3rd Edition - February 15, 2023
  • Latest edition
  • Editor: Ursula Goodenough
  • Language: English

The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1)   The gold-standard reference covering the basic biology of the Chlamydo… Read more

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Description

The Chlamydomonas Sourcebook, 3rd Edition

Introduction to Chlamydomonas and Its Laboratory Use (Volume 1)

The gold-standard reference covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis

Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use has been fully revised and updated to include a wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence.

This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists.

Key features

  • Remains the only complete reference to provide both the historical background and the most up-to-date information and applications on Chlamydomonas
  • Includes best practices for applications in research, including methods for culture, genetic analysis, genomic and transcriptomic analysis, and mutant screening
  • Helps researchers solve common laboratory problems, provides details on the properties of particular strains, and offers a comprehensive survey of molecular approaches
  • Provides a broad perspective for studies in cell and molecular biology, genetics, plant physiology, and related fields

Readership

Researchers in plant science who study photosynthesis; crop scientists; fertility researchers; mammalian vision researchers; biochemistry researchers; plant physiologists; plant and molecular biologists and even human disease; University and Medical libraries. The book will also be of extreme value to both postdoctoral and graduate students

Table of contents

1. The Genus Chlamydomonas

2. Cell Ultrastructure

3. Cell Walls

4. Functional Genomics

5. The Genome

6. Gene Expression: From Transcription to Alternative Splicing

7. Organelle Heredity

8. Cell Cycle and Circadian Rhythms

9. The Sexual Cycle

10. Multicellular Relatives of Chlamydomonas

11. Growth Techniques

12. Practical Aspects of Mating and Tetrad Analysis

13. Transformation of Nuclear, Chloroplast, and Mitochondrial Genomes

14. Methods for the Localization of Cellular Components

15. Mutagenesis and Genome Resequencing

16. Generation, storage, and utilizations of mutant libraries

17. Reverse Genetics

18. Transgene Design

Product details

  • Edition: 3
  • Latest edition
  • Published: February 15, 2023
  • Language: English

About the editor

UG

Ursula Goodenough

Ursula Goodenough is Professor of Biology Emerita at Washington University in St. Louis. She earned her PhD at Harvard University, where her thesis and postgraduate work focused on the biogenesis and ultrastructure of the Chlamydomonas chloroplast. In 1971, she initiated a career-spanning analysis of the Chlamydomonas sexual cycle and cell wall using a combination of genetics, ultrastructure, and molecular biology. She also used these approaches to study Chlamydomonas cilia and lipid-body formation. Key collaborators included Steven Adair, Patrick Ferris, Sabine Waffenschmidt, James Umen, and Jae-Hyeok Lee. Since retiring in 2017, she has completed a wide-ranging ultrastructural study of lichens.
Affiliations and expertise
Professor Emerita of Biology, Washington University, St. Louis, USA

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