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Techniques in Clinical Chemistry

A Handbook for Medical Laboratory Technicians

1st Edition - January 1, 1954

Author: Frederick N. Bullock

eBook ISBN:

9 7 8 - 1 - 4 8 3 2 - 2 1 2 6 - 7

Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and… Read more

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Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and protocols. The opening chapter covers the fundamental aspects of medical laboratories, including accuracy measures, methods of analysis, buffers, indicators, and valency. These topics are followed by discussions on the procedures for preliminary preparation, primarily for sample preparation and specimen collection. Considerable chapters are devoted to the scrutinized analysis of specific specimen, such as blood, cerebrospinal fluid, feces, gastric sample, and urine. The final chapter discusses technical essentials of renal and hepatic function tests. This handbook is directed toward medical and laboratory technicians and clinicians.

I.—Fundamentals Accuracy and Apparatus Volumetric Analysis Standard Solutions Types of Volumetric Analysis Iodometric Standardization of Solutions To Standardize N/10 Hydrochloric Acid Hydrogen Ion Concentration (pH) Buffers Indicators ValencyII.—Preliminary Preparation Cleaning of Glassware Detergents Collection of Pathological Specimens Blood Urine Feces Cerebrospinal Fluid Gastric Contents Exudates and Transudates Preparation of Protein-Free FiltratesIII.—Examination of Blood Blood-Urea Non-Protein Nitrogen Blood Uric Acid Blood-Glucose Blood-Cholesterol Serum-Calcium Plasma-Chlorides Plasma-Proteins Plasma-Sodium Serum-Magnesium Blood-Iron Serum Inorganic Phosphorus Serum-Potassium Serum-Bromides Serum-Phosphatase Serum Ascorbic Acid Serum-Carbon-Dioxide-Combining Power Detection and Estimation of Serum-BilirubinIV.—Cerebrospinal Fluid Analysis Estimation of Chlorides Urea and Glucose Total Protein 'Globulin' ReactionsV.—Analysis of the Feces Estimation of Fat Detection of Occult Blood Bilirubin UrobilinVI.—Gastric Analysis Estimation of Chlorides Detection of Organic Acids Estimation of Free and Combined Hydrochloric Acid The Fractional Test-MealVII.—Examination of Urine Detection and Estimation of Glucose The Glucose-Tolerance Test Detection of Ketone Bodies Estimation of Urine Chlorides Estimation of Urine Urea Estimation of Urine Diastase Estimation of Urine Ascorbic Acid Proteinuria Indicanuria Bilirubin and Urobilin Blood—Hematuria and Hemoglobinuria Melanogenuria Alkaptonuria PorphyrinuriaVIII.—Tests of Renal and Hepatic Function Tests for Renal Efficiency: Urea Concentration Test Urea Clearance Test Urine Concentration Test Urine Dilution Test Liver Function Tests: Thymol Turbidity Test Thymol Flocculation TestAppendix 1.—Normal Blood Values 2.—Atomic Weights 3.—Calibration of Glassware with Mercury 4.—Dichromate Cleaning Fluid 5.—Preparation of Stop-Cock Grease 6.—Normality of Concentrated Acids and Bases 7.—Accuracy of Volumetric Flasks 8.—Specifications for Graduated Pipettes 9.—Specifications for One-Mark Bulb Pipettes 10.—Specifications for BurettesIndex