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Techniques for Biochemical Analysis
- 1st Edition - April 4, 2024
- Authors: Khursheed Hussain, Sameena Maqbool Lone, Khalid Z. Masoodi, Syed Minu Balkhi
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 1 5 9 1 4 - 5
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 5 9 1 5 - 2
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested pr… Read more
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Request a sales quoteTechniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more.
The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.
- Features step-by-step methods for biochemical analysis of a variety of compounds
- Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas
- Provides detailed instructions on how to prepare buffers, the equipment to be used, and the analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones
- Contains interdisciplinary and adaptable methods and techniques
Researchers in biochemistry, molecular biology, biotechnology and related sciences.Postgraduate students in biochemistry, molecular biology, biotechnology, plant science and agricultural studies, and related areas
- Cover image
- Title page
- Table of Contents
- Copyright
- Preface
- Chapter 1. Introduction to biochemistry
- Abstract
- Historical background
- Various branches of biochemistry
- Chapter 2. Introduction to laboratory procedures
- Abstract
- Instructions to work in the laboratory
- Dos and don’ts in the laboratory
- Laboratory equipment care
- Chapter 3. Preparation of solutions and buffers
- Abstract
- Common definitions
- Preparation of solutions
- Preparation of buffers
- Mechanism of buffering action
- Buffering capacity
- Applications of buffer in chemistry
- Chapter 4. Qualitative analysis of carbohydrates
- Abstract
- Classification of carbohydrates
- Tests for carbohydrates
- Chapter 5. Qualitative analysis of proteins
- Abstract
- Classification of carbohydrates
- Functions of proteins
- Chemical tests for the estimation proteins
- Further reading
- Chapter 6. pH (negative logarithm of hydrogen ion concentration)
- Abstract
- Measurement of pH
- pH indicators
- Measurement of pH
- Chapter 7. Spectrophotometry
- Abstract
- Rationale
- Qualitative photometric assays
- Quantitative photometric assays
- Standard curve
- Measurement of absorbance
- Application of spectrophotometry
- Chapter 8. Estimation of carbohydrates
- Abstract
- Sample extraction for estimation of sugars
- Estimation of total sugars by anthrone method
- Estimation of total sugars by phenol−sulfuric method
- Estimation of reducing sugars by Nelson−Somogyi method
- Estimation of nonreducing sugars
- Estimation of starch by anthrone reagent method
- Estimation of glycogen by anthrone reagent method
- Reference
- Chapter 9. Amino acids and proteins
- Abstract
- Amino acids
- Proteins
- Functions of proteins
- Nitrogen estimation by micro-Kjeldahl method
- Nonprotein nitrogen estimation
- Protein nitrogen estimation
- Amino-nitrogen estimation
- Estimation of amino acids
- Chapter 10. Enzyme assays
- Abstract
- Determination of amylase activity
- Determination of invertase (β-fructofuranosidase) activity
- Determination of ribonuclease activity
- Determination of deoxyribonuclease activity
- Determination of β-glucosidase activity
- Determination of pyruvate decarboxylase activity
- Determination of urease activity
- Determination of lipoxygenase (lipoxidase) activity
- Determination of phospholipase C activity
- Determination of α-galactosidase activity
- Determination of nitrate reductase (NR) activity
- Determination of nitrite reductase activity
- Determination of catalase activity
- Determination of peroxidase activity
- Determination of lactate dehydrogenase activity
- Determination of chitinase activity
- Determination of polygalacturonase activity
- Determination of pectin methyl esterase activity
- Determination of L-phenylalanine ammonia lyase activity
- Determination of tyrosine ammonia lyase activity
- Determination of polyphenol oxidase or DOPA oxidase activity
- Determination of phytase activity
- Determination of malate dehydrogenase activity
- Determination of indoleacetic acid oxidase activity
- Determination of ascorbic acid oxidase activity
- References
- Chapter 11. Vitamin assays
- Abstract
- Estimation of vitamin B1 (thiamine)
- Estimation of Vitamin B2 (riboflavin)
- Estimation of vitamin B3 (niacin)
- Estimation of vitamin B6 (pyridoxine)
- Estimation of Vitamin A (retinol)
- Estimation of Vitamin C (ascorbic acid)
- Estimation of vitamins D2 and D3
- Estimation of Vitamin E (tocopherol)
- Estimation of choline
- Chapter 12. Phenolics
- Abstract
- Estimation of total phenols
- Estimation of ortho-dihydric phenols
- Estimation of flavanols
- Estimation of tannins
- Estimation of lignins
- Estimation of capsaicin
- Chapter 13. Plant pigments
- Abstract
- Estimation of total carotenoids
- Estimation of lycopene
- Estimation of chlorophyll
- Estimation of curcumin
- Estimation of anthocyanin
- Estimation of leuco-anthocyanin
- Reference
- Chapter 14. Plant acids
- Abstract
- Estimation of oxalic acid
- Separation and identification of organic acids using chromatography
- Chapter 15. Antinutritional factors in plants
- Abstract
- Estimation of trypsin inhibitor activity
- Estimation of chymotrypsin activity
- Estimation of α-amylase inhibitor activity
- Estimation of cyanogen’s
- Estimation of Gossypol
- Estimation of phytic acid
- Chapter 16. Toxins
- Abstract
- Estimation of aflatoxins
- Chapter 17. Extraction and identification of polyacetylenes
- Abstract
- Rationale
- Reagents required
- Protocol
- Analysis and statistics
- Chapter 18. Alkaloids
- Abstract
- Estimation of solanine
- Purity assessment of solanine using chromatography
- Chapter 19. Plant hormones
- Abstract
- Estimation of auxins (indole-3-acetic acid)
- Estimation of gibberellins
- Estimation of cytokinins
- Estimation of abscisic acid
- Estimation of ethylene (C2H4)
- References
- Chapter 20. Immunochemical methods
- Abstract
- Enzyme-linked immunosorbent assay
- Chapter 21. Nitrogenase activity
- Abstract
- Estimation of nitrogenase activity
- Chapter 22. Chlorophyll
- Abstract
- Estimation of chlorophyll
- Chapter 23. Proximate analysis
- Abstract
- Determination of dry matter and moisture content
- Determination of ash content
- Determination of acid-soluble and -insoluble ash content
- Determination of crude fiber
- Determination of crude fat
- Determination of crude protein
- Determination of nitrogen-free extract
- Chapter 24. Minerals
- Abstract
- Macronutrients
- Micronutrients
- Estimation of sulfur (S)
- Estimation of phosphorus
- Estimation of calcium
- Estimation of magnesium
- Estimation of potassium
- Estimation of molybdenum
- Estimation of copper
- Estimation of zinc
- Estimation of manganese
- Estimation of iron
- Estimation of boron
- Estimation of cobalt
- Estimation of chlorine
- Estimation of silica
- Estimation of sodium
- References
- Chapter 25. Antinutritional factors in plants
- Abstract
- Nutritional studies by in vitro methods
- Nutritional studies by in vivo methods
- Chapter 26. Antioxidants
- Abstract
- Estimation of FRAP
- Scavenging activity of DPPH radical
- Scavenging activity of ABTS radical
- Chapter 27. Lipids
- Abstract
- Estimation of lipids
- Index
- No. of pages: 270
- Language: English
- Edition: 1
- Published: April 4, 2024
- Imprint: Academic Press
- Paperback ISBN: 9780443159145
- eBook ISBN: 9780443159152
KH
Khursheed Hussain
Khursheed Hussain, has been serving as Assistant Professor at Sher-e-Kashmir University of Sciences & Technology in India for 13 years. He served as Scientific Officer to Vice Chancellor from 2015-2020. He has published more than 120 research articles, 25 review articles and many books. His contribution in the field of research is in varietal and hybrid development as Principal/Co-breeder of 7 vegetable varieties with other varieties in the pipeline. He has been an advisor of more than 50 PG students and has been invited to give many lectures and trainings.
Affiliations and expertise
Assistant Professor, Division of Vegetable Science. Sher-e-Kashmir University of Sciences& Technology, IndiaSL
Sameena Maqbool Lone
Sameena Maqbool Lone is a PhD scholar in the Division of Vegetable Science, Sher-e-Kashmir University of Agricultural Sciences and Technology, India. She holds a Master's degree in Horticulture from SKUAST-K, Shalimar. At the Master's level, she worked on "Heterosis and Combining Ability Studies in Cherry Tomato" and obtained competence in morphological and molecular characterization, as well as numerous plant tissue culture technologies. In her Phd programme, she is working on the “Morpho-Molecular Characterization and Nutraceutical Profiling of different carrot genotypes (Daucus carota L.)”. She has outstanding academic and extracurricular achievements, for which she received a merit scholarship from SKUAST-K during her Bachelor's and Master's degree programmes. She has contributed to over 23 academic papers, 6 books, and more than 100 new gene sequences in Genbank by 2022.
Affiliations and expertise
Division of Vegetable Science, Sher-e- Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, IndiaKM
Khalid Z. Masoodi
Dr. Khalid Zaffar Masoodi is a young scientist who works as an Assistant Professor in the Division of Plant Biotechnology at the Sher-e Kashmir University of Agricultural Sciences and Technology in Kashmir (India). He holds a Master's degree in Biotechnology and a doctorate in Plant Biotechnology. Dr. Masoodi is the recipient of numerous awards, including the SERB Early Career Research Award 2016, the SBUR Travel Award in the United States of America in 2012, and the Society of Endocrinology Journal Award in 2018 at Glasgow United Kingdoms. Dr. Masoodi has a distinguished academic record, having qualified for the CSIR-UGC net in Life Sciences, the DBT-BET JRF, and the Graduate Aptitude Test in Engineering and Sciences (GATE) while doing his Masters in Biotechnology. Dr. Masoodi has filed Six patent applications in last 2 years, has over 12 years of teaching and research experience, and has published numerous scientific articles in the fields of cancer research, human disease research, and biotechnology research. His laboratory (Transcriptomics Laboratory, often known as K-Lab, after the Klenow fragment) at SKUAST-K is fully equipped with fluorescence imaging, real-time PCR, cell culture, and Western blotting including other state-of-the-art equipment. His research interests span multiple dimensions, including transcriptomics of plant stress biology (biotic/abiotic) and cancer biology. Apart from that, he is extensively involved in the research of new drugs from medicinal plants. Additionally, he is a co-author of Advanced Molecular Biology and Biotechnology Methods (Academic Press, 2020).
Affiliations and expertise
Assistant Professor/Junior Scientist at the Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, IndiaSB
Syed Minu Balkhi
Dr. Syed Minu Balkhi holds a PhD in Biochemistry from the University of Kashmir, India. She qualified for the JRF-NET CSIR and obtained a fellowship during her PhD program in 2013. She is currently a Lecturer at Government Boys Higher Secondary School, Humhama, India. Prior to this she was a Post-Doctoral Associate at the Department of Immunology, University of Pittsburgh, USA. She has contributed to various publications.
Affiliations and expertise
Lecturer, Government Boys Higher Secondary School