Techniques for Biochemical Analysis
- 1st Edition - April 4, 2024
- Latest edition
- Authors: Khursheed Hussain, Sameena Maqbool Lone, Khalid Z. Masoodi, Syed Minu Balkhi
- Language: English
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested pr… Read more
Description
Description
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more.
The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.
Key features
Key features
- Features step-by-step methods for biochemical analysis of a variety of compounds
- Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas
- Provides detailed instructions on how to prepare buffers, the equipment to be used, and the analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones
- Contains interdisciplinary and adaptable methods and techniques
Readership
Readership
Researchers in biochemistry, molecular biology, biotechnology and related sciences.Postgraduate students in biochemistry, molecular biology, biotechnology, plant science and agricultural studies, and related areas
Table of contents
Table of contents
1. Introduction to
2. Introduction to laboratory procedures
3. Preparation of solutions and buffers
4. Qualitative analysis of carbohydrates
5. Qualitative analysis of proteins
6. pH (negative logarithm of hydrogen ion concentration)
7. Spectrophotometry
8. Carbohydrates
9. Amino acids and proteins
10. Enzyme assays
11. Vitamin assays
12. Phenolics
13. Plant pigments
14. Plant
15. Antinutritional factors in plants
16. Toxins
17. Extraction and identification of polyacetylenes
18. Alkaloids
19. Plant hormones
20. Immunochemical methods
21. Nitrogenase activity
22. Chlorophyll
23. Proximate analysis
24. Minerals
25. Antinutritional factors in plants
26. Antioxidants
27. Lipids
Product details
Product details
- Edition: 1
- Latest edition
- Published: April 4, 2024
- Language: English
About the authors
About the authors
KH
Khursheed Hussain
SL
Sameena Maqbool Lone
Sameena Maqbool Lone is a biotechnology researcher with a strong focus on vegetable science, molecular biotechnology, and sustainable bioproduction. She is currently a postdoctoral researcher in the Division of Vegetable Science, Sher-e- Kashmir University of Agricultural Sciences and Technology of Kashmir, India. Her current work explores the modulation of inflammatory and oncogenic signalling pathways, positioning plant-derived compounds within the broader framework of translational cancer therapeutics. Her research interests include phytochemical science, molecular diversity analysis, and the application of plant resources for health-related innovation. She holds a number of patents and has co-authored several research papers and books.
KM
Khalid Z. Masoodi
Khalid Z. Masoodi is an Associate Professor and Senior Scientist in the Division of Plant Biotechnology at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India. He is the recipient of a number of awards and holds numerous patents. He has two decades worth of teaching and research experience in the areas of plant biotechnology, molecular biology, cancer research, and human disease research, and has published numerous articles and books. He leads a transcriptomics laboratory and works extensively with fluorescence imaging, real-time PCR, cell culture, and Western blotting platforms. His scientific interests include transcription biology in plant stress responses and cancer, as well as the discovery of new drugs from medicinal plants.
SB