Books in Life sciences

Molecular Basis of Biological Activity

1st Edition
December 2, 2012
K Gaedo
English
eBook
9 7 8 0 3 2 3 1 5 1 0 4 7

Molecular Basis of Biological Activity documents the proceedings of a symposium on the Molecular Basis of Biological Activity held in Caracas, Venezuela, July 11-17, 1971. This was the First Meeting of the Pan-American Association of Biochemical Societies (PAABS), and was organized by the Asociacion Venezolana de Bioquimica. The book begins by presenting a lecture on advances in the study of the mechanism of polysaccharide synthesis. This is followed by studies on rabbit muscle aldolase; the catalytic function of α-glycerolphosphate dehydrogenase; the functional and structural roles of metals in metalloenzymes; and enzyme adaptation in mammals. Separate chapters cover collagen biosynthesis and the mechanisms involved in its regulation; the organization of lipids in bilayers; the behavior of water-lipid interactions; the permease or transport systems in the mitochondrial membrane; and interaction between TTX and STX with isolated nerve membrane constituents. The final chapter examines the coupling of respiration via specific dehydrogenases to the transport of amino acids and many sugars.

Autoradiography for Biologists

1st Edition
December 2, 2012
P Gahan
English
eBook
9 7 8 0 3 2 3 1 6 1 0 5 3

Autoradiography for Biologists discusses the basic and practical aspects of various autoradiography techniques. It is composed of six chapters; each chapter covers a specific form of autoradiography technique. Chapter 1 discusses various theoretical aspects of autoradiography, such as radioactive decay, beta-particle absorption, photographic process, sensitivity, and resolving power. Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro-, stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography. Practical and theoretical details of the technique, together with modifications made, are covered in each chapter. This book will be both an encouragement and a help to people wanting to apply autoradiography to the solution of biological problems.

The Biological Fractionation of Isotopes

1st Edition
December 2, 2012
Eric Galimov
English
eBook
9 7 8 0 3 2 3 1 5 7 8 5 8

The Biological Fractionation of Isotopes focuses on the biological fractionation of isotopes and presents calculations of the thermodynamic isotopic beta factor for polyatomic carbon compounds. This book provides experimental and theoretical evidence of the phenomenon of thermodynamically ordered distribution of isotopes in biological systems. This book consists of 12 chapters and opens with an overview of the causes of fractionation of isotopes, along with concepts such as isotopic composition and isotopic effects. The discussion then turns to the isotopic composition of the carbon of organisms; a method of calculating the thermodynamic isotopic factors of polyatomic compounds; and results of experimental investigations of intermolecular and intramolecular isotopic effects. A theoretical model of biological fractionation of isotopes is also described. The following chapters explore the regularities of the biological distribution of isotopes and present the results of some biochemical experiments, including the enzymatic decarboxylation of pyruvate and microbiological oxidation of ethanol to acetic acid. In addition, the geologic aspects of thermodynamically ordered isotopic distributions in biological products are analyzed. This monograph will be of interest to biologists, geochemists, analytical chemists, and geologists.

The Molecular Biology of Ciliated Protozoa

1st Edition
December 2, 2012
Joseph Gall
English
eBook
9 7 8 0 3 2 3 1 5 4 1 8 5

The Molecular Biology of Ciliated Protozoa covers topics that are unique to ciliates, including major molecular progress, genetics, life history, and development of ciliates. Organized into 11 chapters, it focuses on the importance of ciliated protozoa as experimental organisms. The introductory chapter traces the ups and downs of ciliate biology, emphasizing the prominent role of the ciliates in early studies of cell structure, reproduction, and heredity. The book goes on to discuss ciliate genetics and conjugation, providing the basic biological framework for molecular studies of ciliate. Chapters 4 and 5 cover the nuclear DNA content, sequence, and arrangement of holotrichous and hypotrich ciliates. Chapters 6 to 9 examine the characterization of chromosomal telomeres, ribosomal gene amplification, and chromatin and histone structure using ciliated protozoa as experimental organisms. The final two chapters describe the mating mechanism of two ciliates, Blepharisma japonicum and Euplotes raikovi, and the function of surface antigens of Paramecium ciliate. The book is intended for students and investigators who want to learn more about the ciliated protozoa, particularly, in areas that cover fundamental features of eukaryotic biology.

GUS Protocols

1st Edition
December 2, 2012
Sean R. Gallagher
English
eBook
9 7 8 0 3 2 3 1 3 7 6 4 5

The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.

Molecular Cloning and Gene Regulation in Bacilli

1st Edition
December 2, 2012
A Ganesan
English
eBook
9 7 8 0 3 2 3 1 5 3 0 9 6

Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.

Bacillus Molecular Genetics and Biotechnology Applications

1st Edition
December 2, 2012
A.T. Ganesan
English
eBook
9 7 8 0 3 2 3 1 3 8 6 3 5

Bacillus Molecular Genetics and Biotechnology Applications contains the proceedings of the Third International Conference on the Genetics and Biotechnology of Bacilli, held at Stanford University in Stanford, California on July 15-17, 1985. Contributors discuss the progress that has been made concerning the molecular genetics and biotechnology of Bacillus and cover topics related to transposons and plasmids, secretion, gene cloning, and gene expression. This volume is organized into five sections encompassing 39 chapters and begins with an overview of the origin and the state of molecular genetics, along with some of the contributions microbiology has made to fundamental biology. It then emphasizes the importance of scientifically based regulatory decisions and responsible industry actions for effective biotechnology regulation. The chapters that follow focus on Bacillus subtilis, touching on topics such as DNA recombination in plasmids, genetic system for stabilizing cloned genes, regulation of sporulation, and non-complementing diploids. The reader is methodically introduced to the secretion and maturation of subtilisin, cloning in streptomycetes, and genetic exchange and prospects for cloning in Clostridia. The book concludes with a chapter that describes an integrative and amplifiable secretion vector using the inducible promoter and signal peptide from the B. subtilis levansucrase (LS) gene. This book will be of interest to geneticists, microbiologists, and biotechnologists, as well as students and researchers in the fields of molecular biology and biochemistry.

Genetics and Biotechnology of Bacilli

1st Edition
December 2, 2012
A.T. Ganesan
English
eBook
9 7 8 0 3 2 3 1 3 8 6 4 2

Genetics and Biotechnology of Bacilli contains the proceedings of the Second International Conference on Genetics and Biotechnology of Bacilli, held at Stanford University in Stanford, California, on July 6-8, 1983. Contributors discuss the progress that has been made concerning the genetics and biotechnology of Bacillus and focus on topics built around the themes of chromosomal organization, secretion, transcription, gene cloning, gene expression, and synthesis of sporulation-associat... products. This text is organized into 33 chapters and begins with an overview of bacteriophage lambda biology, with emphasis on lambda insertion, controlled DNA rearrangements, operator-promoter function, and the evolution of extrachromosomal elements. The reader is then introduced to genetic mapping of cloned ribosomal RNA genes, gene amplification in Bacillus subtilis, beta-lactamases of Bacilli, and the role of a Bacillus secretion vector in the secretion of foreign gene products. This book also gives an account of various facets of Bacillus biology, especially in the identification of promoters, cloning of foreign genes, and selection of expressed gene products. This reference material is a valuable resource for geneticists, microbiologists, and biotechnologists, as well as students and researchers in the fields of molecular biology and biochemistry.

Genetics and Biotechnology of Bacilli, Volume 2

1st Edition
December 2, 2012
A.T. Ganesan
English
eBook
9 7 8 0 3 2 3 1 3 8 7 1 0

Genetics and Biotechnology of Bacilli, Volume 2 is a collection of papers from the "Fourth International Conference on Bacilli" held in California on June 21-24, 1987. One paper reviews the results of cloning and characterization of genes for secreted enzyme and of genes that control the expression of secreted enzymes in relation with other prokaryotic regulatory systems. Other papers tackle the regulation of gene expression during sporulation, the sigma factors, bacterial toxins, and antibiotic resistance genes. One paper reports that three genes responsible for the code for peptides found in BT cuboidal crystals have been successfully cloned. The three codes have different toxic characteristics in relation to tobacco hornworm larvae or mosquito larvae. Other papers examine replication. Such examination pertains to the two levels of control on the chromosome involved in DNA replication, or to the possible functional importance that several membranes associated DNA subcomplexes can have in Bacillus subtilis, where one of these appear to control initiation. Other papers discus secretion and extracellular enzymes, as well as, the different genetic systems and methods. This collection can prove beneficial for biochemists, micro-biologists, cellular researchers, and academicians involved in the study of cellular biology, microchemistry, or toxicology.

Genetics and Biotechnology of Bacilli

1st Edition
December 2, 2012
Mark M. Zukowski
English
eBook
9 7 8 0 3 2 3 1 5 0 6 2 0

Genetics and Biotechnology of Bacilli, Volume 3 covers the proceedings of the Fifth International Conference on Genetics and Biotechnology of Bacilli, held on July 9-12, 1989 at the Asilomar Conference Center, Pacific Grove, California. It summarizes the remarkable progress made in the genetics and biotechnology fields of Bacilli. It is organized into four parts, encompassing 43 chapters, which focus on gene regulation and structure, enzyme structure, Bacillus thuringiensis toxins, and stationary phase gene regulation. Part I covers topics related to gene regulation and structure of Bacilli, such as control of gene expression, mutation, genetic organization, DNA sequence analysis, and identification of transcript units. It also discusses gene replication in Bacillus subtilis plasmids, levanase operon of B. subtilis, and characterization of global regulon in B. subtilis. The next part of this book focuses on the structure of various enzymes found in B. subtilis, including alpha amylases, subtilisin, alkaline phosphatase, and levansucrase. Part III discusses the generation of functional B. thuringiensis toxin hybrid genes, regulation of crystal protein gene promoters, toxicity of B. thuringiensis delta-endotoxin, and insecticidal activity of chimeric protoxins. The concluding part covers the aspects of signal transduction, regulation of differential gene expression during B. subtilis sporulation, and gene cloning and deletion for extracellular proteases of B. subtilis. It also discusses genetic and biochemical aspects of protein phosphorylation; properties of B. subtilis spores; control of stationary phase gene expression; and the novel regulatory gene, senS, of B. subtilis. This book is a valuable source of information for microbiologists, research biologists, and Bacilli enthusiasts.

Related subjects

Agricultural and biological sciences

Drug discovery

Immunology

Microbiology and virology

Neuroscience

Pharmaceutical sciences

Pharmacology

Toxicology

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Books in Life sciences

Molecular Basis of Biological Activity

Autoradiography for Biologists

The Biological Fractionation of Isotopes

The Molecular Biology of Ciliated Protozoa

GUS Protocols

Molecular Cloning and Gene Regulation in Bacilli

Bacillus Molecular Genetics and Biotechnology Applications

Genetics and Biotechnology of Bacilli

Genetics and Biotechnology of Bacilli, Volume 2

Genetics and Biotechnology of Bacilli

Related subjects