GUS Protocols
Using the GUS Gene as a Reporter of Gene Expression
- 1st Edition - March 10, 1992
- Latest edition
- Editor: Sean R. Gallagher
- Language: English
The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Th… Read more
The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay.
GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.
- First book on the GUS reporter system
- Up-to-date reference lists following each of the fourteen chapters
- Comb-bound for convenient bench-top use
- Written by leading authorities including R.A. Jefferson, inventor of the GUS assay
- Illustrated with color
- GUS detection by fluorometric, spectrophotometric, and histochemical methods
- Fast, automated assays
Graduate students and researchers in molecular biology, biotechnology, molecular genetics, biochemistry, animal cell biology and developmental biology, and plant science
(Section Headings): The GUS Reporter Gene System. The GUS Assay. Histochemical Detection of GUS. Applications of GUS to Plant Genetic Analysis. Applications of GUS to Animal Genetic Anaylsis. Appendices. Index.
- Edition: 1
- Latest edition
- Published: March 10, 1992
- Language: English
SG
Sean R. Gallagher
Affiliations and expertise
Hoefer Scientific Instruments, San Francisco, California, U.S.A.Read GUS Protocols on ScienceDirect