Some Biological Techniques in Electron Microscopy
- 1st Edition - January 1, 1970
- Imprint: Academic Press
- Editor: D.F. Parsons
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 5 4 5 5 5 0 - 3
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 3 3 7 1 2 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 6 0 3 5 - 3
Some Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential… Read more
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Request a sales quoteSome Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential of electron microscope for solving several pressing medical and biological research problems. Organized into five chapters, this book starts with an overview of the primary goal of biological electron microscopy, which is the visualization of atoms in biological molecules and structures. This text proceeds with a discussion of the freezing methods of electron microscopy in which some of the artifacts producing steps of environmental techniques are eliminated. Other chapters consider the objective of electron microscopy to resolve successively small objects, in the limiting instance, single atoms in amorphous structures. The final chapter deals with processing tissues for electron microscopy wherein temperature, agitation, pressure, and humidity can be controlled throughout the operation. Biologists, biophysicists, physicists, electron microscopists, and medical researchers will find this book extremely useful.
List of Contributors
Preface
I. Problems in High Resolution Electron Microscopy of Biological Materials in Their Natural State
I. Introduction
II. Resolution Limitations of Present Day Electron Microscopes
III. Biological High Voltage (1 MeV) Electron Microscopy and Reduction of Specimen Damage
IV. New Methods of Obtaining Contrast
V. Conclusions
References
II. Chemical Effects of Fixation on Biological Specimens
I. Introduction
II. Osmium Tetroxide Fixation
III. Permanganate Fixation
IV. Recent Techniques of Specimen Preparation
References
III. Present Status of Freezing Techniques
I. Introduction
II. Physical Basis of Freezing Techniques
III. Processing for Examination
IV. Conclusions
References
IV. Reducing the Effect of Substrate Noise in Electron Images of Biological Objects
I. Introduction
II. Effect of Substrate on Image
III. Effect of Beam Potential
IV. Substrate Structure
V. Self-Supporting Films
VI. Ideal Substrate
VII. Optical Means for Removing Noise
VIII. Conclusion
References
V. Automation in Tissue Processing for Electron Microscopy
I. Tissue Processors
II. Fixation, Dehydration, and Embedding
III. Future Prospects
References
Author Index
Subject Index
- Edition: 1
- Published: January 1, 1970
- No. of pages (eBook): 198
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780125455503
- Paperback ISBN: 9780124337121
- eBook ISBN: 9780323160353
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