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Solvent Interaction Analysis

Physical Chemistry and Analytical Applications of Aqueous Two-Phase Systems

  • 1st Edition - May 1, 2027
  • Latest edition
  • Authors: Boris Y. Zaslavsky, Vladimir N. Uversky
  • Language: English

Solvent Interaction Analysis: Physical Chemistry and Analytical Applications of Aqueous Two-Phase Systems explores Solvent Interaction Analysis (SIA) technology, a cuttin… Read more

Description

Solvent Interaction Analysis: Physical Chemistry and Analytical Applications of Aqueous Two-Phase Systems explores Solvent Interaction Analysis (SIA) technology, a cutting-edge approach for protein characterization and proteomics in biological fluids. The book considers a broad spectrum of topics, from fundamental principles such as phase separation, blood-tissue distribution, and posttranslational protein modifications to advanced clinical applications for early detection of various malignancies, neurological disorders, and other diseases. The book opens with an introduction to aqueous two-phase system (ATPS), considering different types of ATPS, hydrophobic and electrostatic properties, and mechanisms of liquid-liquid phase separation, before moving on to specific properties of phases such as solute-water interaction and blood-tissue distribution, and analysis of partitioning individual proteins. It then explores solvent interaction analysis, investigating diagnostic applications and biomarker discovery. Final chapters explore fundamental consequences of ATPS-based analysis and its future opportunities.
This comprehensive guide offers a detailed overview of aqueous two-phase systems from foundational knowledge to the latest developments in the field, and is an essential reference for students, researchers and practitioners across a wide range of disciplines, including biophysics, analytical and medicinal chemistry, proteomics, structural biology, pharmaceutical science, and biotechnology.

Key features

  • Explores real-world biomedical applications of aqueous two-phase systems, focusing on biomarker discovery and disease detection
  • Offers an interdisciplinary approach to solvent interaction analysis, integrating biophysics and physical chemistry
  • Investigates mechanisms of phase separation and its applications in biological and clinical contexts
  • Bridges foundational knowledge with advanced practical insights to enhance understanding

Readership

Academic and industrial researchers, advanced students, and scientists working with protein analysis and biomarker discovery across biophysics, physical chemistry, biochemistry, pharmaceutical science, biotechnology and related fields

Table of contents

1. Aqueous two-phase systems

2. Properties of phases

3. Analysis of partitioning of individual proteins

4. Solvent interaction analysis

5. Fundamental consequences of the ATPS-based analysis

6. Biological liquid-liquid phase separation, membrane-less organelles, and biomolecular condensate

Product details

  • Edition: 1
  • Latest edition
  • Published: May 1, 2027
  • Language: English

About the authors

BZ

Boris Y. Zaslavsky

Boris Y. Zaslavsky is Chief Scientific Officer at Cleveland Diagnostics, Inc., which he co-founded in 2013. He trained as a bioanalytical chemist at Moscow State University before joining the USSR Academy of Sciences. He moved to the United States in 1991 to take up a visiting scholar position at Cornell Medical College in New York City, and later worked at KV Pharmaceuticals and Argonne National Laboratory. In 1996, he co-founded Analiza, Inc. Dr. Zaslavsky developed analytical applications of extraction in aqueous two-phase systems and pioneered Solvent Interaction Analysis, used for quality control of biopharmaceuticals and for clinical testing aimed at early cancer detection. He has authored numerous research papers and reviews, as well as one previous book.

Affiliations and expertise
Chief Scientific Officer, Cleveland Diagnostics, Inc., Cleveland, Ohio, USA

VU

Vladimir N. Uversky

Prof. Vladimir N. Uversky, PhD, DSc, FRSB, FRSC, F.A.I.M.B.E., Professor at the Department of Molecular Medicine, Morsani College of Medicine, University of South Florida (USF), is a pioneer in the field of protein intrinsic disorder. He has made a number of groundbreaking contributions in the field of protein folding, misfolding, and intrinsic disorder. He obtained his PhD from Moscow Institute of Physics and Technology and D.Sc. from the Institute of Experimental and Theoretical Biophysics, Russian Academy of Sciences. Since 2010, Professor Uversky has worked at University of South Florida, where he works on various aspects of protein intrinsic disorder phenomenon and analysis of protein folding and misfolding processes. He has authored over 1250 scientific publications and edited several books and book series on protein structure, function, folding, misfolding, and intrinsic disorder. He also servs as an editor in a number of scientific journals.

Affiliations and expertise
Professor, Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, USA