Sertoli Cell Biology
- 2nd Edition - November 20, 2014
- Latest edition
- Editor: Michael D. Griswold
- Language: English
Sertoli Cell Biology, Second Edition summarizes the progress since the last edition and emphasizes the new information available on Sertoli/germ cell interactions. This inform… Read more
Sertoli Cell Biology, Second Edition summarizes the progress since the last edition and emphasizes the new information available on Sertoli/germ cell interactions. This information is especially timely since the progress in the past few years has been exceptional and it relates to control of sperm production in vivo and in vitro.
- Fully revised
- Written by experts in the field
- Summarizes 10 years of research
- Contains clear explanations and summaries
- Provides a summary of references over the last 10 years
Research scientists in the field of reproductive biology, urologists, andrologists, and endocrinologists
List of contributors
Preface
References
1. Sertoli cell anatomy and cytoskeleton
I Introduction
II Sertoli cell morphology
III Sertoli cell cytoskeleton
IV Concluding remarks
References
2. Establishment of fetal Sertoli cells and their role in testis morphogenesis
I Introduction
II Establishment of the gonadal primordium
III Sertoli cell specification and diversion of molecular development toward the testis pathway
IV Organizational functions of fetal Sertoli cells
Summary
References
3. Early postnatal interactions between Sertoli and germ cells
I Introduction
II Neonatal testis development
III Role of Sertoli cells in gonocyte proliferation and migration
IV Role of Sertoli cells in formation of primary undifferentiated and differentiating spermatogonial populations
V Concluding remarks
References
4. The spermatogonial stem cell niche in mammals
I Research advances related to the mammalian SSC niche since 2003
II Principles of stem cell niches in mammalian tissues
III Spermatogonial stem cells
IV Location of the SSC
V Factors governing SSC self-renewal and differentiation
VI The environment inside and outside the niche
VII The role of cell migration in SSC self-renewal and differentiation
VIII Spermatogonial differentiation
IX Niche localization: what controls the controller?
X The SSC niche and the cycle of the seminiferous epithelium
XI The SSC niche during cell loss
XII Perspectives
Acknowledgments
References
5. DMRT1 and the road to masculinity
I Introduction
II DMRT1 expression
III Regulation of DMRT1
IV DMRT1 locus and gene expression
V DMRT1 function
VI Conclusions
References
6. Hormonal regulation of spermatogenesis through Sertoli cells by androgens and estrogens
I Introduction
II Androgen signaling
III Testosterone production and action
IV Androgen receptor
V The role of androgens in Sertoli cells
VI AR-dependent gene expression in Sertoli cells
VII Sertoli cell estrogen signaling (from androgens via aromatase)
VIII Conclusions and future perspectives
References
7. Activins and inhibins in Sertoli cell biology: Implications for testis development and function
I Introduction: activin and inhibin link multiple cell types to determine male reproductive health
II General structure and signaling pathways
III Regulation of inhibin and activin production
IV Activin and inhibin function in the adult testis
V Activin and inhibin in the developing testis
VI The contribution of Smads to regulation of testis development and growth
VII Clinical relevance of activin and inhibin for male reproduction
VIII Concluding remarks: the need to understand signaling crosstalk in the testis
Acknowledgments
References
8. The initiation of spermatogenesis and the cycle of the seminiferous epithelium
I Introduction and highlights since the last volume
II Differentiation of spermatogonia
III Evidence that RA is required for the initiation of meiosis
IV The initiation of asynchronous spermatogenesis by RA
V Regulation of RA synthesis and degradation in the developing testis
VI Extrinsic versus intrinsic factors
References
9. Retinoic acid metabolism, signaling, and function in the adult testis
I Introduction
II RA synthesis, signaling, and degradation
III Components of the RA pathway within the adult testis
IV Maintenance of the spermatogenic cycle by RA
V Sertoli cell contributions to RA function within the adult testis
VI The effects of retinoids on Sertoli cell function
VII Conclusions and remaining questions
References
10. Stage-specific gene expression by Sertoli cells
I Introduction
II Evidence that spermatogenic cells regulate biologically important, stage-specific functions of Sertoli cells
III CTSL, a model for the analysis of the function and regulation of stage-specific gene expression
IV Stage-specific gene expression as a fundamental characteristic of Sertoli cells
V Future directions
Acknowledgments
References
11. MicroRNAs and Sertoli cells
I Noncoding RNAs
II miRNAs
III The role of miRNAs in spermatogenesis in vivo
IV SC-expressed miRNAs and their functions
V Regulation of SC-expressed miRNAs
VI Perspective
References
12. Biochemistry of Sertoli cell/germ cell junctions, germ cell transport, and spermiation in the seminiferous epithelium
I Introduction
II Cell junctions and their restructuring during the epithelial cycle in the testis
III Ectoplasmic specialization
IV Spermatid transport and spermiation
V Transport of preleptotene spermatocytes at the BTB
VI Concluding remarks and future perspectives
Acknowledgments
References
13. Sertoli cell structure and function in anamniote vertebrates
I Introduction
II Sertoli cell proliferation
III Sertoli cell functions
IV Concluding remarks
References
14. Adult Sertoli cell differentiation status in humans
I Introduction and scope of the chapter
II Development of the adult Sertoli cell population
III Proliferation and differentiation around puberty
IV Differentiation in adult life
V Human Sertoli cell differentiation and pathology
VI Future perspectives
References
15. Gene knockouts that affect Sertoli cell function
I Introduction
II Genes identified as essential for normal Sertoli cell development and function through KO studies
III Lessons learned from the gene KO studies
IV Approaches to gene ablation in Sertoli cells
V Conclusions and perspectives
Acknowledgments
References
Preface
References
1. Sertoli cell anatomy and cytoskeleton
I Introduction
II Sertoli cell morphology
III Sertoli cell cytoskeleton
IV Concluding remarks
References
2. Establishment of fetal Sertoli cells and their role in testis morphogenesis
I Introduction
II Establishment of the gonadal primordium
III Sertoli cell specification and diversion of molecular development toward the testis pathway
IV Organizational functions of fetal Sertoli cells
Summary
References
3. Early postnatal interactions between Sertoli and germ cells
I Introduction
II Neonatal testis development
III Role of Sertoli cells in gonocyte proliferation and migration
IV Role of Sertoli cells in formation of primary undifferentiated and differentiating spermatogonial populations
V Concluding remarks
References
4. The spermatogonial stem cell niche in mammals
I Research advances related to the mammalian SSC niche since 2003
II Principles of stem cell niches in mammalian tissues
III Spermatogonial stem cells
IV Location of the SSC
V Factors governing SSC self-renewal and differentiation
VI The environment inside and outside the niche
VII The role of cell migration in SSC self-renewal and differentiation
VIII Spermatogonial differentiation
IX Niche localization: what controls the controller?
X The SSC niche and the cycle of the seminiferous epithelium
XI The SSC niche during cell loss
XII Perspectives
Acknowledgments
References
5. DMRT1 and the road to masculinity
I Introduction
II DMRT1 expression
III Regulation of DMRT1
IV DMRT1 locus and gene expression
V DMRT1 function
VI Conclusions
References
6. Hormonal regulation of spermatogenesis through Sertoli cells by androgens and estrogens
I Introduction
II Androgen signaling
III Testosterone production and action
IV Androgen receptor
V The role of androgens in Sertoli cells
VI AR-dependent gene expression in Sertoli cells
VII Sertoli cell estrogen signaling (from androgens via aromatase)
VIII Conclusions and future perspectives
References
7. Activins and inhibins in Sertoli cell biology: Implications for testis development and function
I Introduction: activin and inhibin link multiple cell types to determine male reproductive health
II General structure and signaling pathways
III Regulation of inhibin and activin production
IV Activin and inhibin function in the adult testis
V Activin and inhibin in the developing testis
VI The contribution of Smads to regulation of testis development and growth
VII Clinical relevance of activin and inhibin for male reproduction
VIII Concluding remarks: the need to understand signaling crosstalk in the testis
Acknowledgments
References
8. The initiation of spermatogenesis and the cycle of the seminiferous epithelium
I Introduction and highlights since the last volume
II Differentiation of spermatogonia
III Evidence that RA is required for the initiation of meiosis
IV The initiation of asynchronous spermatogenesis by RA
V Regulation of RA synthesis and degradation in the developing testis
VI Extrinsic versus intrinsic factors
References
9. Retinoic acid metabolism, signaling, and function in the adult testis
I Introduction
II RA synthesis, signaling, and degradation
III Components of the RA pathway within the adult testis
IV Maintenance of the spermatogenic cycle by RA
V Sertoli cell contributions to RA function within the adult testis
VI The effects of retinoids on Sertoli cell function
VII Conclusions and remaining questions
References
10. Stage-specific gene expression by Sertoli cells
I Introduction
II Evidence that spermatogenic cells regulate biologically important, stage-specific functions of Sertoli cells
III CTSL, a model for the analysis of the function and regulation of stage-specific gene expression
IV Stage-specific gene expression as a fundamental characteristic of Sertoli cells
V Future directions
Acknowledgments
References
11. MicroRNAs and Sertoli cells
I Noncoding RNAs
II miRNAs
III The role of miRNAs in spermatogenesis in vivo
IV SC-expressed miRNAs and their functions
V Regulation of SC-expressed miRNAs
VI Perspective
References
12. Biochemistry of Sertoli cell/germ cell junctions, germ cell transport, and spermiation in the seminiferous epithelium
I Introduction
II Cell junctions and their restructuring during the epithelial cycle in the testis
III Ectoplasmic specialization
IV Spermatid transport and spermiation
V Transport of preleptotene spermatocytes at the BTB
VI Concluding remarks and future perspectives
Acknowledgments
References
13. Sertoli cell structure and function in anamniote vertebrates
I Introduction
II Sertoli cell proliferation
III Sertoli cell functions
IV Concluding remarks
References
14. Adult Sertoli cell differentiation status in humans
I Introduction and scope of the chapter
II Development of the adult Sertoli cell population
III Proliferation and differentiation around puberty
IV Differentiation in adult life
V Human Sertoli cell differentiation and pathology
VI Future perspectives
References
15. Gene knockouts that affect Sertoli cell function
I Introduction
II Genes identified as essential for normal Sertoli cell development and function through KO studies
III Lessons learned from the gene KO studies
IV Approaches to gene ablation in Sertoli cells
V Conclusions and perspectives
Acknowledgments
References
- Edition: 2
- Latest edition
- Published: November 20, 2014
- Language: English
MG
Michael D. Griswold
Dr. Michael D. Griswold, Regents Professor of Molecular Biosciences at Washington State University (WSU), is world-renowned for his contributions to our understanding of Sertoli cell structure and functions and the crucial role of Vitamin A in spermatogenesis and a loyal member of SSR. Mike received a B.S. in Chemistry and Ph.D. in Biochemistry from the University of Wyoming. He conducted postdoctoral studies with Dr. Philip Cohen at the University of Wisconsin, funded by an NIH Postdoctoral Fellowship, working on amphibian metampophosis, and with Dr. Flauco P. Tocchini-Valentini in Rome, funded by an EMBO Fellowship, on Xenopus RNA. Mike spent a year as an Assistant Professor of Pharmacology at Baylor College of Medicine in Houston. He then spent two years as a Research Associate in the laboratory of Dr. Irving Fritz at the University of Toronto, where he acquired what would become a life-long interest in Sertoli cells. Mike joined the faculty of WSU in 1976 as an Assistant Professor of Biochemistry and Basic Medical Sciences, was promoted to Associate and then full Professor, appointed Chair of the Department of Biochemistry and Biophysics in 1994, Director of the School of Molecular Biosciences in 1999, and Dean of the College of Sciences, a position he held from 2003-2010. Mike became a Regents Professor of Molecular Biosciences in 2008, and in 2011, received the WSU Eminent Faculty Award, the highest faculty honor at WSU.
Mike is an exemplary mentor. He trained 7 MS students, 24 graduate students that include Mike Skinner, Ken Roberts and Leslie Heckert, and 26 postdoctoral fellows and research associates that include Carlos Morales, Kwan Hee Kim, Carol Linder, Dereck McLean, and Cathryn Hogarth. Mike received the Frontiers in Reproduction Beacon Award in 2008 for his outstanding mentoring. One of his former students, Leslie Heckert wrote: “His high expectations returned dedication, creativity and independence from the members of his laboratory, which in turn produced a vibrant and exciting research environment. He led by example, support and occasionally by providing unsolicited words of encouragement...”.
Mike’s lab pioneered microarray/gene profiling analyses of FSH-treated Sertoli cells, beginning in 2002, followed by analysis of spermatogonial stem cells, testis, seminiferous epithelium, epididymis, spermatogenesis, and embryonic development of the testis. These databases are freely available to the scientific community. The number of investigators across the world that have used these data bases is remarkable.
As evidenced from the comments above, Mike has been at the forefront of research in male reproductive biology for the past three decades. Leslie Heckert wrote: “His ideas, approaches, and results have enhanced our understanding of Sertoli cell and gamete function, improved animal models and methodologies for the study of spermatogenesis, and precipitated new concepts in testis biology, contraception and infertility. Because of Dr. Griswold, more is known about the role of retinoic acid in regulating meiosis and the cycle of the seminiferous epithelium, the actions of FSH and testosterone, Sertoli cell function, and the characteristics of spermatogonial stem cells.”
Michael Griswold meets and exceeds the criteria for the Hartman Award in terms of his original research, mentoring of many successful scientists, foresight generosity to the field, and leadership. He has made SSR proud. (Submitted by Mary Hunzicker-Dunn, Ph.D.).
Affiliations and expertise
School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.Read Sertoli Cell Biology on ScienceDirect