Separation of Cells and Subcellular Elements
Proceedings of a Meeting Organised by EFRAC (European Working Party for the Separation and Detection of Biological Fractions), Sponsored by the Committee for Science and Technology of the Council of Europe, Brussels, 4-5 May 1979
- 1st Edition - November 13, 2013
- Latest edition
- Editor: H. Peeters
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 1 - 7 3 5 4 - 2
- eBook ISBN:9 7 8 - 1 - 4 8 3 1 - 8 9 3 5 - 2
Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent… Read more
Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent suspension, the phase partition of cells and subcellular particles, and the continuous free-flow electrophoresis. The first chapters deal with flow cytometric characterization of tumor associated changes in gynecologic malignancies; steady state rheolectrolysis; and electrophoretic approaches applicable to cell separation. The succeeding chapters consider the chromosome separation by velocity sedimentation at unit gravity and the separation of human lymphocytes based on volume and density. Other chapters describe the analysis of the gradient former for zonal rotors and the centrifugal elutriation of living cells. The last chapters are devoted to the human platelet isolation from whole blood on metrizamide gradients. The book can provide useful information to biochemists, cytologists, students, and researchers.
Preface
General Problems
Chemical Engineering Approaches in Bio-separation Methods
Cellular Aggregation in Turbulent Suspensions
Flow Cytometry
Flow Cytometric Characterization of Tumor Associated
Changes in Gynecologic Malignancies
Phase Partition
Phase Partition of Cells and Subcellular Particles
Electrophoresis
Continuous Free-flow Electrophoresis
New Electrophoretic Approaches Applicable to Cell Separation
Steady State Rheolectrolysis. II. Experimental Endeavor
Unit Gravity Sedimentation
A Sorter of Cells, Chromosomes and Nuclei that Combines
Simplicity with Good Resolution
Chromosome Separation by Velocity Sedimentation at Unit Gravity
Utracentrifugal Methods
Elutriation
Centrifugal Elutriation: Separation of Living Cells
Density Gradients
A New Gradient Former for Zonal Rotors
The Use of Density Gradients of Percoll® for the Separation of Biological Particles
Improved Separation of Human Peripheral T-cells Using
PVP-coated Colloidal Silica Particles (Percoll)
Separation of Human Lymphocytes on the Basis of Volume and Density
Human Platelet Isolation from Whole Blood on Metrizamide Gradients
Isolation of Dense Bodies from Human Blood Platelets using Discontinuous Metrizamide Gradients
Exchange of Cholesterol Between the Subcellular Fractions
During Differential Centrifugation of Rat Adrenocortical Tissue
The Isolation of Stable Cattle Rod Outer Segments with an Intact Plasma Membrane
Continuous Flow
Studies on Large Scale Preparation of Polymerized Human Red
Cells Ghosts able to Neutralize Blood Groups Antibodies
Large-scale Concentration and Purification of Various Animal
Viruses Using Isopycnic Banding in a Continuous-flow Ultracentrifuge
Index
- Edition: 1
- Latest edition
- Published: November 13, 2013
- Language: English
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