Scientific Principles of Adipose Stem Cells
- 1st Edition - August 19, 2021
- Imprint: Academic Press
- Editors: Lauren Kokai, Kacey Marra, J. Peter Rubin
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 1 9 3 7 6 - 1
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 1 9 3 7 7 - 8
Scientific Principles of Adipose Stem Cells provides readers with in-depth and expert knowledge on adipose stem cells, their developmental biologic origins, foundational research… Read more
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Request a sales quoteScientific Principles of Adipose Stem Cells provides readers with in-depth and expert knowledge on adipose stem cells, their developmental biologic origins, foundational research on ASC signaling mechanisms and immunomodulatory properties, and clinical insights into applications in regenerative medicine. Topics covered include basic adipose stem cell developmental biology and mechanisms of regulating self-renewal and activation in the stem cell niche, important methods for isolation and characterizing ASCs, and data on the impact on human demographics (age, sex, BMI) on ASC phenotype. A section devoted to ASC biology, ASCs for stem cell therapy and regenerative medicine, and ASCs in tissue engineering applications are also included.
The book is written for scientists and clinicians who are broadly familiar with stem cells and basic cell biology principles and those seeking advanced information on adipose stem cells.
- Coverage of basic adipose stem cell developmental biology (maturation process during embryogenesis) and mechanisms of regulating self-renewal and activation in the stem cell niche
- Includes important methods for isolation and characterizing ASCs, as well as known data any impact of human demographics (age, sex, BMI) on ASC phenotype
- An entire section dedicated to ASC biology, additional sections will be devoted to ASCs for stem cell therapy and regenerative medicine, as well as ASCs in tissue engineering applications
Academics and clinicians working with adipose tissue and adipose stem cells. Also, likely includes companies working to commercialize stem cell products. This may include (but is not limited to) stem cell biologists, tissue engineers, plastic surgeons. Many Universities feature tissue engineering and stem cell courses. This book will likely be purchased as a physical copy and/or e-book to support these classes
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Contributors
- Preface
- Part 1: Introduction and front matter
- Chapter 1: Plastic surgery, fat, and fat plasticity: How adipose tissue changed the landscape of stem cell therapeutics
- Abstract
- Plastic surgery and fat plasticity
- Part 2: Basic biology of adipose stem cells
- Chapter 2: Developmental origins of adipocytes: What we learn from human pluripotent stem cells
- Abstract
- Introduction: Three types of adipocytes in mammals
- Developmental origins of white adipocytes
- Lineage tracing studies reveal a complex situation for the developmental origins of adipocytes
- Human models to investigate the developmental origins of adipocytes
- hiPSC-3D adipospheres: The next steps toward gaining greater insight into the development of human brown-like adipocytes
- Importance of matching the embryonic origin between transplanted cells and the host environment for tissue regeneration: A lesson for the selection of the best donor fate site?
- Chapter 3: Establishing the adipose stem cell identity: Characterization assays and functional properties
- Abstract
- Introduction
- Physical characterization
- Multipotent differentiation
- Characterization of in vitro immunomodulatory abilities
- Conclusion and future directions
- Chapter 4: Insights into the adipose stem cell niche in health and disease
- Abstract
- Acknowledgments and funding support
- Competing interests
- Introduction
- Defining adipocyte-derived stem cells and progenitors
- Generation of adipocytes from adipocyte precursors
- Adipocyte precursors reside in the stromal vasculature fraction
- Immunophenotyping of ASCs and APs
- Adipocyte precursors in the native niche
- Cell interactions within the adipose precursor niche
- Fibroblast growth factor signaling and renewal of adipocyte precursors
- Bone morphogenetic proteins and commitment of adipogenic precursors
- Adipose-derived stem cells express core regulators of self-renewal and pluripotency
- Changes in adipose-derived stem cells during obesity and metabolic disease
- Changes in adipocyte progenitors during aging may contribute to metabolic dysfunction
- Conclusions and future perspectives
- Chapter 5: Mechanisms of adipose-derived stem cell aging and the impact on therapeutic potential
- Abstract
- Introduction
- General aging background
- Adipose-derived stem cell aging mechanisms
- How adipose-derived stem cell aging impacts current clinical application of adipose-derived stem cells
- Current molecular attempts at rejuvenating stem cells for clinical applications
- Summary
- Chapter 6: Human pluripotent nontumorigenic multilineage differentiating stress enduring (Muse) cells isolated from adipose tissue: A new paradigm in regenerative medicine and cell therapy
- Abstract
- Acknowledgments
- Discovery of Muse and adipose-derived multilineage-differentiating stress-enduring cells
- Components of adipose tissue
- Adipose stem cells
- Adipose-derived Muse cells vs adipose stem cells
- Properties of adipose-derived Muse cells
- Conclusions
- Chapter 7: Adipose stem cell homing and routes of delivery
- Abstract
- Introduction
- Homing steps
- Strategies to enhance homing of ASCs
- Routes of MSC delivery
- Current challenges in adipose stem cell homing therapy
- Chapter 8: Bioreactors and microphysiological systems for adipose-based pharmacologic screening
- Abstract
- Introduction
- Components of adipose-based microphysiological systems and bioreactors
- Current adipose-based microphysiological systems/bioreactors
- Assessments of microphysiological systems and bioreactors
- Future directions
- Part 3: Adipose cell therapy and regenerative medicine
- Chapter 9: Adipose stem cells and donor demographics: Impact of anatomic location, donor sex, race, BMI, and health
- Abstract
- Introduction
- Patient demographics
- Effects of patient health
- Clinical translation
- Chapter 10: Immunomodulatory properties of adipose stem cells in vivo: Preclinical and clinical applications
- Abstract
- Introduction
- Transplantation
- Autoimmune disorders
- Other conditions
- Conclusions and future directions
- Chapter 11: Clinical experience with adipose tissue enriched with adipose stem cells
- Abstract
- A brief history
- The value of fat
- Adipose composition
- Adipose-derived stem cells
- Tools and techniques
- Risks and complications
- Indications
- On the horizon: Clinical trials
- Summary
- Chapter 12: Adipose-derived stem cells for wound healing and fibrosis
- Abstract
- Introduction
- Skin: The largest organ of the body
- Modern therapeutic approaches
- Regenerative properties of the adipose-derived stem cell
- Evidence of adipose-derived stem cells in wound healing and fibrosis
- Future prospects for use of adipose-derived stem cells in wound healing and fibrosis
- Conclusions
- Chapter 13: Oncologic safety of adipose-derived stem cell application
- Abstract
- Interaction of adipose-derived stem cells and breast cancer at a cellular level
- Clinical studies on oncologic safety of fat grafting
- Oncologic safety of enriched fat grafting to the breast
- Guidelines and recommendations on fat grafting
- Conclusion
- Chapter 14: FDA regulation of adipose cell use in clinical trials and clinical translation
- Abstract
- Adipose: Composition and functions
- A quick primer: Legislation, regulations and subregulatory guidance
- The FDA's current enforcement strategy for adipose HCT/Ps
- Clarifying terms
- Conclusion
- Part 4: Engineering with adipose stem cell
- Chapter 15: Biomaterial control of adipose-derived stem/stromal cell differentiation
- Abstract
- Part 1: General introduction
- Part 2: Properties of biomaterial scaffolds for guided differentiation
- Part 3: Controlled release systems for guided differentiation
- Part 4: Combining ASCs with biomaterials for cell-based therapies
- Part 5: Discussion and conclusions
- Chapter 16: Genetic modification of adipose-derived stem cells for bone regeneration
- Abstract
- Financial disclosure statement
- Introduction
- In vitro differentiation
- Approaches for gene alterations
- Clinical applications
- Future directions and conclusions
- Chapter 17: Adipose-derived stromal/stem cells for bone tissue engineering applications
- Abstract
- Acknowledgments
- Enhancing bone wound healing: From native grafts, to material- and cell-based tissue engineering approaches
- Bone repair using bone marrow or adipose-derived mesenchymal stromal/stem cells
- Refining engineering strategies for optimal osteogenesis and functionality
- Enhancing bone formation with bone morphogenetic proteins
- Clinical applications of adipose-derived stromal/stem cells and adipose-derived stromal/stem cell-based engineered products for bone repair
- Perspectives for next-generation bone tissue engineering: Biofabrication of personalized constructs
- Conclusion
- Chapter 18: The hematopoietic potential of stem cells from the adipose tissue
- Abstract
- Introduction: adipose tissue as a reservoir for stromal and hematopoietic stem cells
- Adipose-derived stromal stem cells
- Hematopoietic stem cells
- Conclusion
- Chapter 19: Adipose stem cells for peripheral nerve engineering
- Abstract
- Introduction to adipose-derived stem cells in peripheral nerve injury
- Composition of the peripheral nervous system and adipose-derived stem cell crossover
- Adipose-derived stem cells and the rationale for use in peripheral nerve injury
- In vivo studies: Adipose-derived stem cells in the peripheral nervous system
- Clinical trials and future considerations
- Conclusions and future directions
- Index
- Edition: 1
- Published: August 19, 2021
- Imprint: Academic Press
- No. of pages: 484
- Language: English
- Paperback ISBN: 9780128193761
- eBook ISBN: 9780128193778
LK
Lauren Kokai
Dr. Kokai is co-director of the Adipose Stem Cell Center at the University of Pittsburgh,
faculty of the McGowan Institute of Regenerative Medicine (MIRM) and research faculty member in the Department of Plastic Surgery, School of Medicine. Dr. Kokai has a broad background in soft tissue engineering with specific training in development of novel biomaterials and drug delivery systems for adipose tissue regeneration and use of mesenchymal stem cells for tissue engineering and cell therapy applications. She has extensive experience in developing bioassays for biologic product development and has led collaborations with industry partners to transition preclinical research into commercialized products. Dr. Kokai is a co-Investigator in an NIH funded line of research aimed at developing cell-based methods for clinical soft tissue reconstruction after cancer therapy.
Affiliations and expertise
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USAKM
Kacey Marra
Dr. Marra is an Associate Professor in the Department of Plastic Surgery (Primary), and Department of Bioengineering (Secondary), at the University of Pittsburgh. Dr. Marra’s academic training was in the areas of polymer chemistry, biomaterials and tissue engineering. She is the Director of the Plastic Surgery Research Laboratory (since 2002) and a core faculty member in the McGowan Institute for Regenerative Medicine, where she has served as a member of the Executive Committee since 2004. She has a publication record of 114 peer-reviewed publications, 24 reviews, 14 book chapters, and 4 patents, and over 570 abstracts in the areas of nerve regeneration, adipose stem cells, bioreactors, tissue engineering, and wound healing. Her funding includes NIH, NSF, and DoD grants in the area of regenerative medicine (including the Armed Forces Institute for Regenerative Medicine, (AFIRM)). She is the Enabling Technology Core Focus Area Leader in AFIRM II. She was Co-Chair of the 2015 Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, held in Boston.
Affiliations and expertise
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USAJR
J. Peter Rubin
Dr. Rubin is endowed Professor of Plastic Surgery and Professor of Bioengineering, at the University of Pittsburgh with a faculty appointment with the McGowan Institute of Regenerative Medicine (MIRM). Dr. Rubin developed a basic science research program in the biology of adipose-derived stem cells and serves as Co-Director of the Adipose Stem Cell Center at the University of Pittsburgh. Dr. Rubin holds leadership positions in national and international plastic surgery societies, is the principal investigator in an NIH-funded line of research aimed at developing cell based methods for clinical soft tissue reconstruction after cancer therapy and holds two FDA cell therapy IDE protocols and an IND, all as sponsor/ investigator. Dr. Rubin’s many scientific leadership positions include past president of the International Society of Adipose Therapeutics and Science and past Chairman of the Plastic Surgery Research Council.
He has received numerous awards for research, including the highly competitive Gingrass Award from the Plastic Surgery Research Council. He is the recipient of the American Association of Plastic Surgeons Academic Scholar Award, and the Plastic Surgery Educational Foundation Award for Outstanding Achievement in Basic and Translational Science. In 2007, Dr. Rubin was selected to receive a Presidential Early Career Award for Scientists and Engineers (PECASE). Dr. Rubin contributes expertise in scientific models assessing the use of adult adipose stem cell therapies for tissue engineering and regenerative applications
Affiliations and expertise
Director of Body Contouring Program, Associate Professor of Surgery, Chief, Division of Plastic and Reconstructive Surgery Chief and Endowed Professor Department of Plastic and Reconstructive SurgeryRead Scientific Principles of Adipose Stem Cells on ScienceDirect