Scale-up and Chemical Process for Microbial Production of Plant-Derived Bioactive Compounds

From Laboratory to Industry

1st Edition - March 20, 2024
Editors: Yongjun Wei, Xiao-Jun Ji, Mingfeng Cao
Language: English
Paperback ISBN:
9 7 8 - 0 - 4 4 3 - 1 5 5 8 4 - 0
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 1 5 5 8 5 - 7

Many plant-derived bioactive compounds are the foundation for drugs or effective drugs to cure diseases. Usually, the bioactive compounds in plant biomass are low, and the… Read more

Scale-up and Chemical Process for Microbial Production of Plant-Derived Bioactive Compounds

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Many plant-derived bioactive compounds are the foundation for drugs or effective drugs to cure diseases. Usually, the bioactive compounds in plant biomass are low, and the extraction of bioactive compounds from plants is not eco-friendly, which limits their application in drugs and other industries. While the biosynthesis of some complex bioactive compounds is possible, the scale-up and chemical process technology still needs further application in commercial production of the plant-derived bioactive compounds.

Scale-up and Chemical Process for Microbial Production of Plant-Derived Bioactive Compounds: From Laboratory to Industry summarizes recent commercial microbial production strategies for plant-derived bioactive compounds. This comprehensive summary will be of great interest to scientists and researchers, as well as engineers who work on the microbial production of plant-derived bioactive compounds in the industry.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Chapter 1. Advances and prospects for advanced biomanufacturing
Abstract
Introduction
Optimization and scale-up of fermentation process based on process parameter correlation analysis
Key technologies and applications of optimization and scale-up of biological fermentation process based on process model
Rational scale-up methodology combining cellular physiology and flow field of biochemical reactor
Intelligent biomanufacturing is a strong driving force for the transformation and upgrading of bioeconomy
Acknowledgment
References
Chapter 2. Different microbial strategies used for industrial-scale production of plant-derived bioactive compounds
Abstract
Metabolic engineering facilitates de novo synthesis
Typical steps
Gene identification and characterization
Suitable microbial host
Introduction of target gene
Optimization of target gene expression level
Metabolic network modification
Positive strains selection
Scale-up fermentation
Enzyme engineering for continuous biotransformation
Enzyme engineering
Identification of target enzyme with desired properties
Mutant library generation
High-throughput screening
Processing technology
Whole-cell catalysis
Cell-free catalysis
References
Chapter 3. Optimization parameters for efficient scale-up of fermentation process
Abstract
Introduction
Fermentation process characterization
Process data acquiring, fusing, and visualization
Laboratory scale-down tools for efficient and rational scale-up
Emerging trends and future directions
References
Chapter 4. Development of economic extraction and purification process for plant-derived bioactive compounds
Abstract
Introduction
Separation and purification methods for bioactive compounds
Existing problems and prospects
Acknowledgment
Declaration of interest
References
Chapter 5. Screening and characterization of probiotics for large-scale production of plant-derived prebiotics
Abstract
Methods for screening probiotics for plant fermentation
Generation of prebiotics from engineered microbes via plant biomass fermentation
Isolation of bioactive compounds
Commercial production of plant-derived bioactive compounds
Perspective
Acknowledgments
Declaration of interest
References
Chapter 6. Designing and engineering synthetic microbiota to utilize plant lignin-based biomass for the synthesis of bioactive compounds
Abstract
Introduction
Upstream processing of lignin
Downstream bioprocessing of lignin
Conclusion and outlook
Acknowledgments
Author contributions
Competing financial interests
References
Chapter 7. The principles to design and optimization of industrial bioprocesses
Abstract
Introduction
Typical modes of bioprocess used in production
Basic principles for bioprocess design
Acknowledgments
Declaration of interests
References
Chapter 8. The synthetic probiotic microbiota and their potential applications in the production of plant-derived products
Abstract
Introduction
Probiotics derived from fermented foods
Synthetic probiotic microbiota
Production of plant-derived products using synthetic probiotic microbiota
Conclusions and perspectives
Acknowledgments
Declaration of interests
References
Chapter 9. Fermentation utilizing engineered microbes: revolutionizing the production of commercial products from plant-derived bioactive compounds
Abstract
Abbreviations
Introduction
Commonly used engineered microorganisms for plant bioactive compound synthesis
Advances in engineering metabolic pathways for plant bioactive compound synthesis
Factors impacting the fermentation process of plant-derived bioactive compounds
Three main modes: batch, fed-batch, and continuous fermentation
Fermentation techniques: solid-state fermentation and submerged fermentation
Challenges ahead for the microbial synthesis of plant bioactive compounds
Summary and future prospects
References
Chapter 10. Examples for successful commercial production of plant-derived bioactive compounds
Abstract
Introduction
Ginsenoside
β-Nicotinamide mononucleotide
Artemisinin
Conclusion
Acknowledgments
Conflict of interest
References
Chapter 11. Production of synthetic edible oils with engineered yeasts: from lab to commercialization
Abstract
Introduction
Yeasts are potential edible oil producer
The lipid biosynthetic pathway of yeasts
Cocoa butter production in yeasts
Omega-3 production in yeasts
Several companies focusing on synthetic oil and other food products
Perspective
Acknowledgments
Declaration of interests
References
Index

Yongjun Wei

Dr. Yongjun Wei, PhD, completed his bachelor’s degree in biology at Shaanxi Normal University, China, before pursuing his PhD in microbiology from the Chinese Academy of Sciences (CAS) in 2014. Following his doctoral studies, he conducted a postdoctoral fellowship at the Chalmers University of Technology from 2014 to 2016. After that, he worked as an assistant researcher at CAS for a year before joining Zhengzhou University’s faculty in 2018. Currently he serves as an associate professor at Zhengzhou University, Dr. Wei has published over 80 peer-reviewed papers and holds more than 20 patents. Furthermore, he serves as an editorial board member or youth editor for over 10 different journals. His research primarily focuses on microbial production of plant bioactive natural products and the utilization of microorganisms for disease treatment and pollution eradication.

Affiliations and expertise

School of Pharmaceutical Sciences, Laboratory of Synthetic Biology, Food Laboratory of Zhongyuan, Zhengzhou University, Zhengzhou, China

Xiao-Jun Ji

Dr. Xiao-Jun Ji, PhD, is currently a professor of bioengineering and biochemical engineering at Nanjing Tech University (NanjingTech), China. He received his BSc and PhD degrees from NanjingTech in 2005 and 2009 and conducted the visiting research in the Systems and Synthetic Biology lab headed by Prof. Jens Nielsen at Chalmers, Sweden during 2016 and 2017. His recent research focuses on biomanufacturing pharmaceutical and nutritional chemicals using the nonconventional yeasts through the metabolic engineering and the emerging synthetic biology tools. He has many awards such as the Fok Ying-Tung Foundation Young Scholars Award (2014), the Hou De-Bang Chemical Youth Award of CIESC (2016), the National Technological Invention Award of China (2018), the Excellent Young Scholars of NSFC (2019), and the Newton Advanced Fellowships of the Royal Society (2020).

Affiliations and expertise

NanjingTech University, National Engineering Research Centre for Biotechnology , Nanjing, China

Mingfeng Cao

Dr. Mingfeng Cao, PhD, is the Minjiang distinguished professor in the Department of Chemical and Biochemical Engineering, the director of the Institute of Bioengineering, and an honorary research fellow of the Tan Kah Kee Innovation Laboratory at the Xiamen Laboratory, China. He was awarded his PhD from the Nankai University in 2011, and between 2011 and 2013 worked as a research associate at the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences. He completed postdoctoral studies at Iowa State University, USA and was a research scientist at the University of Illinois from 2018 to 2021. His research focuses on the development of synthetic biology and metabolic engineering strategies to establish microbial cell factories for value-added and green chemical production. Dr. Cao has published over 60 journal articles and holds 10 patents. He also serves as the editor-in-chief of Biotechnology Reports and the associate editor for journals such as Microbial Cell Factories and Biotechnology for Biofuels and Bioproducts.

Affiliations and expertise

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, China