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Rodak's Hematology
Clinical Principles and Applications
- 7th Edition - April 15, 2024
- Editors: Elaine M. Keohane, Michelle Montgomery Preston, Kamran M. Mirza, Jeanine M. Walenga
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 3 6 5 0 - 7
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 3 4 9 1 6 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 3 7 6 2 - 7
Make sure you are thoroughly prepared to work in a clinical laboratory. Rodak’s Hematology: Clinical Principles and Applications, 7th Edition uses hundreds of full-color photomicr… Read more
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Request a sales quoteMake sure you are thoroughly prepared to work in a clinical laboratory. Rodak’s Hematology: Clinical Principles and Applications, 7th Edition uses hundreds of full-color photomicrographs to help you understand the essentials of hematology and hemostasis. This new edition details the parts and functions of the cell; shows how to accurately identify cells; covers normal hematopoiesis through diseases of erythroid, myeloid, lymphoid, and megakaryocytic origins; and simplifies hemostasis and thrombosis concepts and disorders. Easy to follow and understand, this book also covers key topics, including working in the hematology and hemostasis laboratory; complementary testing areas such as flow cytometry, cytogenetics, and molecular diagnostics; and laboratory testing of blood cells and body fluid cells.
- Cover image
- Title page
- Table of Contents
- Copyright
- Tribute
- Dedications
- Reviewers
- Contributors
- Preface
- Part I. Introduction to Hematology
- 1. An Overview of Clinical Laboratory Hematology
- HISTORY
- RED BLOOD CELLS
- WHITE BLOOD CELLS
- PLATELETS
- COMPLETE BLOOD COUNT
- BLOOD FILM EXAMINATION
- ENDOTHELIAL CELLS
- COAGULATION
- ADVANCED HEMATOLOGY PROCEDURES
- ADDITIONAL HEMATOLOGY PROCEDURES
- QUALITY ASSURANCE AND QUALITY CONTROL
- SAFETY
- 2. Patient Safety in Hematology and Hemostasis
- DEFINITION OF PATIENT SAFETY
- DEFINITION OF HEALTH CARE QUALITY
- TOTAL TESTING PROCESS IN THE CLINICAL LABORATORY
- SIX AIMS OF HEALTH CARE QUALITY
- ESSENTIAL COMPETENCIES FOR HEALTH CARE PROFESSIONALS
- APPLICATIONS IN THE HEMATOLOGY AND HEMOSTASIS LABORATORY
- SUMMARY
- 3. Quality Assurance in Hematology and Hemostasis Testing
- STATISTICAL SIGNIFICANCE AND EXPRESSIONS OF CENTRAL TENDENCY AND DISPERSION
- METHOD VALIDATION
- MULTISITE VALIDATION
- LOT-TO-LOT COMPARISONS
- REFERENCE INTERVAL AND THERAPEUTIC RANGE DEVELOPMENT
- INTERNAL QUALITY CONTROL
- EXTERNAL QUALITY ASSESSMENT
- ASSESSING DIAGNOSTIC EFFICACY
- RECEIVER OPERATING CHARACTERISTIC CURVE
- ASSAY FEASIBILITY
- LABORATORY STAFF COMPETENCE
- QUALITY ASSURANCE PLAN: PREANALYTICAL AND POSTANALYTICAL
- AGENCIES THAT ADDRESS HEMATOLOGY AND HEMOSTASIS QUALITY
- SUMMARY
- Part II. Blood Cell Production, Structure, and Function
- 4. Cell Structure and Function
- CELL ORGANIZATION
- PLASMA MEMBRANE
- NUCLEUS
- CYTOPLASM
- SELECTED CELL PROCESSES
- HEMATOPOIETIC MICROENVIRONMENT
- 5. Hematopoiesis
- HEMATOPOIETIC DEVELOPMENT
- ADULT HEMATOPOIETIC TISSUE
- HEMATOPOIETIC STEM CELLS AND CYTOKINES
- LINEAGE-SPECIFIC HEMATOPOIESIS
- THERAPEUTIC APPLICATIONS
- SUMMARY
- 6. Erythrocyte Production and Destruction
- ERYTHROPOIESIS
- DYNAMICS OF RED BLOOD CELL PRODUCTION AND DESTRUCTION
- MICROENVIRONMENT OF THE BONE MARROW
- ERYTHROCYTE DESTRUCTION
- SUMMARY
- 7. Erythrocyte Metabolism and Membrane Structure and Function
- ERYTHROCYTE METABOLISM (ENERGY PRODUCTION THROUGH ANAEROBIC GLYCOLYSIS)
- GLYCOLYSIS DIVERSION PATHWAYS (SHUNTS)
- RED BLOOD CELL MEMBRANE STRUCTURE AND FUNCTION
- SUMMARY
- 8. Hemoglobin Metabolism
- HEMOGLOBIN STRUCTURE
- HEMOGLOBIN BIOSYNTHESIS
- HEMOGLOBIN ONTOGENY
- REGULATION OF HEMOGLOBIN PRODUCTION
- HEMOGLOBIN FUNCTION
- DYSHEMOGLOBINS
- HEMOGLOBIN MEASUREMENT
- SUMMARY
- 9. Iron Kinetics and Laboratory Assessment
- IRON CHEMISTRY
- IRON KINETICS
- DIETARY IRON, BIOAVAILABILITY, AND DEMAND
- LABORATORY ASSESSMENT OF BODY IRON STATUS
- 10. Leukocyte Development, Kinetics, and Functions
- GRANULOCYTES
- MONONUCLEAR CELLS
- 11. Platelet Production, Structure, and Function
- MEGAKARYOCYTOPOIESIS
- PLATELETS
- PLATELET ULTRASTRUCTURE
- PLATELET FUNCTION
- PLATELET SIGNALING PATHWAYS
- PLATELETS AND INFLAMMATION
- PLATELET PROTEOME
- SUMMARY
- Part III. Laboratory Evaluation of Blood Cells
- 12. Manual, Semiautomated, and Point-of-Care Testing in Hematology
- SPECIMEN COLLECTION
- MANUAL CELL COUNTS
- HEMOGLOBIN DETERMINATION
- MICROHEMATOCRIT
- RULE OF THREE
- RED BLOOD CELL INDICES
- RETICULOCYTE COUNT
- ERYTHROCYTE SEDIMENTATION RATE
- ADDITIONAL TESTS FOR RED BLOOD CELLS
- POINT-OF-CARE TESTING
- SUMMARY
- 13. Automated Blood Cell Analysis
- GENERAL PRINCIPLES OF AUTOMATED BLOOD CELL ANALYSIS
- PRINCIPAL ANALYZERS
- VALIDATION AND CALIBRATION OF AUTOMATED ANALYZERS
- LIMITATIONS AND INTERFERENCES
- CLINICAL UTILITY OF AUTOMATED BLOOD CELL ANALYSIS
- ANALYZER SELECTION
- 14. Examination of the Peripheral Blood Film and Correlation With the Complete Blood Count
- PERIPHERAL BLOOD FILMS
- SUMMARIZING COMPLETE BLOOD COUNT RESULTS
- SUMMARY
- 15. Bone Marrow Examination
- BONE MARROW ANATOMY AND ARCHITECTURE
- INDICATIONS FOR BONE MARROW EXAMINATION
- BONE MARROW SPECIMEN COLLECTION SITES
- BONE MARROW ASPIRATION AND BIOPSY
- PREPARATION OF THE BONE MARROW SPECIMEN
- EXAMINING BONE MARROW ASPIRATE OR IMPRINT
- EXAMINING THE BONE MARROW CORE BIOPSY SPECIMEN
- DEFINITIVE BONE MARROW STUDIES
- BONE MARROW EXAMINATION REPORTS
- SUMMARY
- Part IV. Erythrocyte Disorders
- 16. Anemias: Red Blood Cell Morphology and Approach to Diagnosis
- DEFINITION OF ANEMIA
- IMPORTANCE OF PATIENT HISTORY AND CLINICAL FINDINGS
- PHYSIOLOGIC ADAPTATIONS IN PATIENTS WITH ANEMIA
- MECHANISMS OF ANEMIA
- LABORATORY DIAGNOSIS OF ANEMIA
- APPROACH TO EVALUATING ANEMIAS
- 17. Disorders of Iron Kinetics and Heme Metabolism
- GENERAL CONCEPTS IN IRON-RELATED DISORDERS
- IRON-RESTRICTED ANEMIAS
- PORPHYRIAS
- SIDEROBLASTIC ANEMIAS
- IRON OVERLOAD
- DIFFERENTIAL DIAGNOSIS
- 18. Anemias Caused by Defects of DNA Metabolism
- ETIOLOGY
- SYSTEMIC MANIFESTATIONS OF FOLATE AND VITAMIN B12 DEFICIENCY
- CAUSES OF VITAMIN DEFICIENCIES
- LABORATORY DIAGNOSIS
- MACROCYTIC NONMEGALOBLASTIC ANEMIAS
- TREATMENT
- SUMMARY
- 19. Bone Marrow Failure
- PATHOPHYSIOLOGY OF BONE MARROW FAILURE
- APLASTIC ANEMIA
- OTHER FORMS OF BONE MARROW FAILURE
- 20. Introduction to Increased Destruction of Erythrocytes
- CLASSIFICATION OF HEMOLYTIC DISORDERS
- PHYSIOLOGIC HEMOLYSIS
- PATHOLOGIC HEMOLYSIS
- CLINICAL FEATURES
- LABORATORY FINDINGS
- DIFFERENTIAL DIAGNOSIS
- SUMMARY
- 21. Intrinsic Defects Leading to Increased Erythrocyte Destruction
- RED BLOOD CELL MEMBRANE ABNORMALITIES
- RED BLOOD CELL ENZYMOPATHIES
- 22. Extrinsic Defects Leading to Increased Erythrocyte Destruction—Nonimmune Causes
- MICROANGIOPATHIC HEMOLYTIC ANEMIA
- MACROANGIOPATHIC HEMOLYTIC ANEMIA
- HEMOLYTIC ANEMIA CAUSED BY INFECTIOUS AGENTS
- HEMOLYTIC ANEMIA CAUSED BY OTHER RED BLOOD CELL INJURY
- 23. Extrinsic Defects Leading to Increased Erythrocyte Destruction—Immune Causes
- OVERVIEW OF IMMUNE HEMOLYTIC ANEMIAS
- AUTOIMMUNE HEMOLYTIC ANEMIA
- ALLOIMMUNE HEMOLYTIC ANEMIAS
- DRUG-INDUCED IMMUNE HEMOLYTIC ANEMIA
- 24. Hemoglobinopathies (Structural Defects in Hemoglobin)
- STRUCTURE OF GLOBIN GENES
- HEMOGLOBIN DEVELOPMENT
- GENETIC MUTATIONS
- ZYGOSITY
- PATHOPHYSIOLOGY
- NOMENCLATURE
- HEMOGLOBIN S
- HEMOGLOBIN C
- HEMOGLOBIN C-HARLEM (HEMOGLOBIN C-GEORGETOWN)
- HEMOGLOBIN E
- HEMOGLOBIN O-ARAB
- HEMOGLOBIN D AND HEMOGLOBIN G
- COMPOUND HETEROZYGOSITY WITH HEMOGLOBIN S AND ANOTHER β-GLOBIN GENE MUTATION
- CONCOMITANT CIS MUTATIONS WITH HEMOGLOBIN S
- HEMOGLOBIN M
- UNSTABLE HEMOGLOBIN VARIANTS
- HEMOGLOBINS WITH INCREASED AND DECREASED OXYGEN AFFINITY
- GLOBAL BURDEN OF HEMOGLOBINOPATHIES
- SUMMARY
- 25. Thalassemias
- DEFINITIONS AND HISTORY
- EPIDEMIOLOGY
- GENETICS OF GLOBIN SYNTHESIS
- CATEGORIES OF THALASSEMIA
- GENETIC DEFECTS CAUSING THALASSEMIA
- PATHOPHYSIOLOGY
- β-GLOBIN GENE CLUSTER THALASSEMIA
- α-THALASSEMIA
- THALASSEMIA ASSOCIATED WITH STRUCTURAL HEMOGLOBIN VARIANTS
- DIAGNOSIS OF THALASSEMIA
- Part V. Leukocyte Disorders
- 26. Nonmalignant Leukocyte Disorders
- PRIMARY IMMUNODEFICIENCY DISORDERS/INBORN ERRORS OF IMMUNITY
- LYSOSOMAL STORAGE DISORDERS
- MORPHOLOGIC ABNORMALITIES OF LEUKOCYTES WITHOUT ASSOCIATED IMMUNE DEFICIENCY
- QUANTITATIVE ABNORMALITIES OF LEUKOCYTES
- SECONDARY MORPHOLOGIC CHANGES IN LEUKOCYTES
- INFECTIOUS MONONUCLEOSIS
- 27. Introduction to Hematologic Neoplasms
- GENERAL CHARACTERISTICS OF HEMATOLOGIC NEOPLASMS
- INCIDENCE, PREVALENCE, AND ETIOLOGY
- CLASSIFICATION SCHEMES
- MOLECULAR PATHOGENESIS
- THERAPY
- 28. Flow Cytometric Analysis in Hematologic Disorders
- SPECIMEN PROCESSING
- FLOW CYTOMETRY: PRINCIPLE AND INSTRUMENTATION
- ANALYSIS OF FLOW CYTOMETRIC DATA
- CELL POPULATIONS IDENTIFIED BY FLOW CYTOMETRY
- FLOW CYTOMETRIC ANALYSIS OF MYELOID NEOPLASMS (ACUTE MYELOID LEUKEMIAS AND CHRONIC MYELOID NEOPLASMS)
- FLOW CYTOMETRIC ANALYSIS OF LYMPHOID NEOPLASMS (LYMPHOBLASTIC LEUKEMIA/LYMPHOMA AND MATURE LYMPHOID NEOPLASMS)
- OTHER APPLICATIONS OF FLOW CYTOMETRY BEYOND IMMUNOPHENOTYPING OF HEMATOLOGIC MALIGNANCIES
- 29. Molecular Diagnostics in Hematopathology
- STRUCTURE AND FUNCTION OF DNA
- MOLECULAR DIAGNOSTIC TESTING OVERVIEW
- NUCLEIC ACID ISOLATION
- AMPLIFICATION OF NUCLEIC ACIDS BY POLYMERASE CHAIN REACTION
- DETECTION OF AMPLIFIED DNA
- REAL-TIME POLYMERASE CHAIN REACTION
- NUCLEIC ACID SEQUENCING
- CHROMOSOMAL MICROARRAYS
- PATHOGEN DETECTION AND INFECTIOUS DISEASE LOAD
- CURRENT DEVELOPMENTS
- 30. Cytogenetics
- REASONS FOR CHROMOSOME ANALYSIS
- CHROMOSOME STRUCTURE
- CHROMOSOME IDENTIFICATION
- TECHNIQUES FOR CHROMOSOME PREPARATION AND ANALYSIS
- CYTOGENETIC NOMENCLATURE
- CHROMOSOME ABNORMALITIES
- CANCER CYTOGENETICS
- CHROMOSOMAL MICROARRAY ANALYSIS
- 31. Acute Leukemias
- CLASSIFICATION SCHEMES FOR ACUTE LEUKEMIAS
- ACUTE LYMPHOBLASTIC LEUKEMIA
- ACUTE MYELOID LEUKEMIA
- ACUTE LEUKEMIAS OF AMBIGUOUS LINEAGE
- CYTOCHEMICAL STAINS AND INTERPRETATIONS
- 32. Myeloproliferative Neoplasms
- BCR::ABL1-POSITIVE MYELOPROLIFERATIVE NEOPLASMS
- BCR::ABL1-NEGATIVE MYELOPROLIFERATIVE NEOPLASMS
- OTHER MYELOPROLIFERATIVE NEOPLASMS
- MYELOID/LYMPHOID NEOPLASMS
- MASTOCYTOSIS
- 33. Myelodysplastic Neoplasms
- ETIOLOGY
- MORPHOLOGIC ABNORMALITIES IN PERIPHERAL BLOOD AND BONE MARROW
- DIFFERENTIAL DIAGNOSIS
- ABNORMAL CELLULAR FUNCTION
- CLASSIFICATION OF MYELODYSPLASTIC NEOPLASMS
- MYELODYSPLASTIC NEOPLASMS WITH DEFINING GENETIC ABNORMALITIES
- MYELODYSPLASTIC NEOPLASMS, MORPHOLOGICALLY DEFINED
- CHILDHOOD MYELODYSPLASTIC NEOPLASMS
- MYELODYSPLASTIC/MYELOPROLIFERATIVE NEOPLASMS
- CYTOGENETICS, MOLECULAR ALTERATIONS, AND EPIGENETICS
- PROGNOSIS
- TREATMENT
- 34. Mature Lymphoid Neoplasms
- GENETIC ABNORMALITIES
- CLASSIFICATION SCHEMAS BASED ON CELL DIFFERENTIATION
- APPROACH TO DIAGNOSIS: INTERFACE BETWEEN CLINICAL AND LABORATORY MEDICINE
- DIAGNOSTIC PROCEDURES
- DIAGNOSTIC EVALUATION
- LEUKEMIAS
- NON-HODGKIN LYMPHOMA
- HODGKIN LYMPHOMA
- PLASMA CELL NEOPLASMS
- Part VI. Hemostasis and Thrombosis
- 35. Normal Hemostasis
- OVERVIEW OF HEMOSTASIS
- VASCULAR INTIMA IN HEMOSTASIS
- PLATELETS
- COAGULATION SYSTEM
- COAGULATION REGULATORY MECHANISMS
- FIBRINOLYSIS
- 36. Hemorrhagic Disorders and Laboratory Assessment
- BLEEDING SYMPTOMS
- ACQUIRED COAGULOPATHIES
- CONGENITAL COAGULOPATHIES
- 37. Qualitative Disorders of Platelets and Vasculature
- QUALITATIVE PLATELET DISORDERS: INHERITED DEFECTS
- QUALITATIVE PLATELET DISORDERS: ACQUIRED DEFECTS
- VASCULAR DISORDERS
- 38. Quantitative Disorders of Platelets: Thrombocytopenia and Thrombocytosis
- THROMBOCYTOPENIA: DECREASE IN CIRCULATING PLATELETS
- THROMBOCYTOSIS: INCREASE IN CIRCULATING PLATELETS
- 39. Thrombotic Disorders and Laboratory Assessment
- THROMBOSIS RISK TESTING DEVELOPMENTS
- THROMBOSIS ETIOLOGY AND PREVALENCE
- THROMBOSIS RISK FACTORS
- LABORATORY EVALUATION OF THROMBOPHILIA
- ARTERIAL THROMBOSIS PREDICTORS
- VENOUS THROMBOEMBOLIC DISEASE DIAGNOSIS
- DISSEMINATED INTRAVASCULAR COAGULATION
- HEPARIN-INDUCED THROMBOCYTOPENIA
- THROMBOINFLAMMATION
- THROMBOSIS RISK TESTING FUTURE
- SUMMARY
- 40. Antithrombotic Therapies and Laboratory Assessment
- Thrombosis and Antithrombotic Therapy
- Warfarin
- Unfractionated Heparin
- Low-Molecular-weight Heparin
- Fondaparinux
- Direct Anti-Xa Oral Anticoagulants
- Direct Thrombin Inhibitors
- Switching Anticoagulants and Periprocedural Management
- Antiplatelet Therapy
- Fibrinolytic Therapy
- Future of Antithrombotic Therapy
- Summary
- 41. Laboratory Evaluation of Hemostasis
- HEMOSTASIS LABORATORY TESTING
- HEMOSTASIS SPECIMEN COLLECTION
- HEMOSTASIS SPECIMEN MANAGEMENT
- PLATELET FUNCTION TESTS
- PLATELET ACTIVATION MARKERS
- CLOT-BASED SCREENING TESTS FOR COAGULATION DISORDERS
- COAGULATION FACTOR ASSAYS
- FIBRINOLYSIS ASSAYS
- VISCOELASTOMETRY AND GLOBAL COAGULATION ASSAYS
- GENETIC TESTING FOR HEMOSTATIC DISORDERS
- 42. Hemostasis and Coagulation Instrumentation
- THE CLINICAL COAGULATION LABORATORY
- ASSAY ENDPOINT DETECTION PRINCIPLES
- ADVANTAGES AND DISADVANTAGES OF DETECTION METHODS
- COAGULATION INSTRUMENTATION AND TECHNOLOGY ADVANCEMENTS
- SELECTION OF COAGULATION INSTRUMENTATION
- CURRENTLY AVAILABLE COAGULATION INSTRUMENTS
- POINT-OF-CARE COAGULATION TESTING
- GLOBAL HEMOSTASIS ASSESSMENT
- PLATELET FUNCTION TESTING
- MOLECULAR GENETIC TESTING IN COAGULATION
- Part VII. Hematology and Hemostasis in Selected Populations
- 43. Hematology and Hemostasis in the Pediatric, Pregnant, Geriatric, and Transgender Populations
- PEDIATRIC HEMATOLOGY AND HEMOSTASIS
- GESTATIONAL HEMATOLOGY AND HEMOSTASIS
- GERIATRIC HEMATOLOGY AND HEMOSTASIS
- TRANSGENDER POPULATIONS
- ECHAPTERS (EVOLVE)
- E1. Safety in the Hematology Laboratory
- STANDARD PRECAUTIONS
- OCCUPATIONAL HAZARDS
- E2. Blood Specimen Collection
- SAFETY
- RESPONSIBILITY OF THE PHLEBOTOMIST IN INFECTION CONTROL
- PHYSIOLOGIC FACTORS AFFECTING TEST RESULTS
- VENIPUNCTURE
- SKIN PUNCTURE
- QUALITY ASSURANCE IN BLOOD SPECIMEN COLLECTION
- SPECIMEN HANDLING
- LEGAL ISSUES IN PHLEBOTOMY
- SUMMARY
- E3. Care and Use of the Microscope
- PRINCIPLES OF MICROSCOPY
- COMPONENT PARTS AND THEIR FUNCTIONS
- OPERATING PROCEDURE WITH KOEHLER ILLUMINATION
- IMMERSION OIL AND TYPES
- CARE OF THE MICROSCOPE
- BASIC TROUBLESHOOTING
- OTHER MICROSCOPES USED IN THE CLINICAL LABORATORY
- SUMMARY
- E4. Body Fluid Analysis in the Hematology Laboratory
- PERFORMING CELL COUNTS ON BODY FLUIDS
- PERFORMING DIFFERENTIAL COUNTS ON BODY FLUIDS
- CEREBROSPINAL FLUID
- SEROUS FLUID
- SYNOVIAL FLUID
- BRONCHOALVEOLAR LAVAGE SPECIMENS
- SUMMARY
- APPENDICES (EVOLVE)
- Appendix A. Abbreviations
- Appendix B. List of Formulas
- Appendix C. Answers
- Appendix D. Glossary
- INDEX
- Hematology/Body Fluids/Hemostasis Reference Intervals
- No. of pages: 928
- Language: English
- Edition: 7
- Published: April 15, 2024
- Imprint: Elsevier
- Paperback ISBN: 9780323936507
- eBook ISBN: 9780443349164
- eBook ISBN: 9780323937627
EK
Elaine M. Keohane
Affiliations and expertise
Professor Emeritus, Clinical Laboratory and Medical Imaging Sciences, School of Health Professions, Rutgers, The State University of New Jersey, Newark, New JerseyMM
Michelle Montgomery Preston
Affiliations and expertise
Associate Professor, Pathology, Anatomy, and Laboratory Medicine, Vice Chair, Biomedical Laboratory Diagnostics Program Director, Medical Laboratory Science, School of Medicine, West Virginia University, Morgantown, West VirginiaKM
Kamran M. Mirza
Affiliations and expertise
Clinical Professor of Pathology, Director, Division of Education Programs, Pathology, Michigan Medicine, Ann Arbor, MichiganJW
Jeanine M. Walenga
Affiliations and expertise
Professor, Thoracic-Cardiovascular Surgery, Pathology, and Physiology, Co-Director, Hemostasis and Thrombosis Research Unit, Stritch School of Medicine, Loyola University Chicago, Laboratory Director, Clinical Coagulation, Laboratory Director, Urinalysis and Medical Microscopy, Associate Director, Point of Care & Referred Testing, Pathology and Laboratory Medicine, Loyola University Health System, Maywood, Illinois