RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways
- 1st Edition, Volume 449 - December 15, 2008
- Latest edition
- Editors: Lynne E. Maquat, Megerditch Kiledjian
- Language: English
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic… Read more
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Description
Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.
Key features
Key features
- Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway
- Expert researchers introduce the most advanced technologies and techniques
- Offers step-by-step lab instructions, including necessary equipment and reagents
Readership
Readership
Biochemists and researchers in related life science disciplines.
Table of contents
Table of contents
Methods to Study No-Go Decay in Saccharomyces cerevisiaeCell-Cycle Regulation of Histone mRNA Degradation in Mammalian Cells: Role of Translation and Oligouridylation Assays of ARE-Mediated mRNA Decay in CellsEvaluating the Control of mRNA Decay in Fission YeastIn Vivo Analysis of the Decay of Transcripts Generated by Cytoplasmic RNA VirusesQualitative and quantitative assessment of the activity of the yeast NMD pathwayNonsence Mediated mRNA Decay in C. elegansIn vivo Analysis of Plant Nonsense-Mediated Decay. Estimating Nuclear mRNA Decay in S. CerevisiaeIdentification and Analysis of tRNAs that are Degraded in Saccharomyces cerevisiae Due to Lack of ModificationsAnalysis of Non-Functional rRNA Decay in Saccharomyces cerevisiae Identifying Substrates of mRNA Decay Factors by a Combined RNA Interference and DNA Microarray ApproachAnalysis of RNA-Protein Interactions Using a Yeast Three-Hybrid SystemCo-Immunoprecipitation Techniques for Assessing RNA-Protein Interactions in vivoHow to Define Targets for Small Guide RNAs in RNA Silencing: A Biochemical ApproachRapid mRNA Deadenylation and Decay Triggered by MicroRNAsA Method to Detect miRNA Targets Using miRNA as a Primer
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 449
- Published: December 15, 2008
- Language: English
About the editors
About the editors
LM
Lynne E. Maquat
Affiliations and expertise
University of Rochester, NY, USAMK
Megerditch Kiledjian
Affiliations and expertise
Rutgers University, New Brunswick, NJ, USAView book on ScienceDirect
View book on ScienceDirect
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