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Elsevier

RNA-binding Proteins Part C

  • 1st Edition, Volume 745 - March 15, 2027
  • Latest edition
  • Editors: Subashchandrabose Chinnathambi, David Christianson, Karen N. Allen
  • Language: English

This volume brings together a comprehensive collection of contemporary methods and applications for investigating RNA biology, RNA–protein interactions, RNA-binding proteins (RBPs… Read more

Description

This volume brings together a comprehensive collection of contemporary methods and applications for investigating RNA biology, RNA–protein interactions, RNA-binding proteins (RBPs), non-coding RNAs, RNA processing, phase separation, ribosome biogenesis, and RNA-based regulation and therapeutics. As Volume 744 of the Methods in Enzymology series, it emphasizes the integration of methodological innovation with biological applications, providing researchers with practical approaches to understand how RNA–protein interactions regulate RNA stability, localization, translation, processing, packaging, and cellular function. A major strength of the volume is its coverage of emerging and rapidly developing areas of RNA research, including RNA–protein condensates and phase separation, circular RNAs, RNA-binding domains, RNA editing, miRNA trafficking, and RNA-based therapeutics. The chapters provide complementary perspectives spanning computational prediction, molecular and cellular biology, structural biology, advanced imaging, genetics, proteomics, and disease-relevant cellular models.

Collectively, the volume serves as a multidisciplinary methodological resource for researchers investigating RNA, RNA-binding proteins, and RNA-regulated cellular processes. By bridging computational, biochemical, structural, and cellular approaches, it provides a valuable framework for understanding the complex mechanisms governing RNA function and RNA–protein interactions and supports the development of new strategies for studying RNA biology in health and disease.

Key features

  • Computational Prediction and Structural Analysis of RNA–Protein Interactions
  • RNA–Protein Interactions and RNA Cargo Trafficking
  • RNA Processing, Non-Coding RNAs, and RNA Regulation
  • miRNA Biology and RNA Packaging
  • RNA–Protein Condensates and Phase Separation
  • Features high-impact reviews exploring the latest discoveries and methodologies in RNA-binding protein research

Readership

Students, academicians, clinicians

Table of contents

1. Intrinsically disordered RNA-binding motifs cooperate to catalyze RNA folding and drive phase separation
Dierk Niessing and Thomas Monecke

2. Imaging protocols and quantitative analysis of biomolecular condensates formed by RNA binding proteins
Graciela Lidia Boccaccio

3. Integration of Protein-Protein and RNA-Protein Interactions to Identify RNP complexes
Ruthrotha Selvi Bharathavikru, Nishad Gosavi and Sourav Dey

4. Isolation and functional characterisation of Tau containing phagosomes in human iPSC-derived microglia
Subashchandrabose Chinnathambi and Tanu Suma Narayanappa

5. Microexon Identification and Splicing Regulation through RNA transcriptome Analysis
Bikash Choudhary

6. Computational predictions and analysis of RBP interaction with coding and noncoding RNAs
Amaresh Chandra Panda

7. Histoblotting the brain: mapping regional protein expression in unfixed neural tissue
Rafael Lujan

8. A quantitative analysis to determine heterogeneity of stress granules in arsenite-induced oxidative stress
Chandrama Mukherjee and Anakshi Gayen

9. Aminoacyl-tRNA synthetases
Amit Sharma, Bipasa Dey and Nachiappan Mutharasappan

10. Liquid-liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau
Susmitha Ambadipudi

11. Predictions of disordered RNA-binding regions with one of our state-of-the-art tools Hybrid RNA bind or DeepDISObind
Lukasz Kurgan

12. RNA-binding proteins in AD
Mireya Plass

13. Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries
Arnaud Hubstenberger

14. Regulatory RNAs and its gene expression
Vidisha Tripathi

15. mRNA Tethered Ribosome Interactome Profiling (mTRIP): A method for Identifying Trans-Acting Factors on Ribosome-Bound mRNA in Yeast
Pankaj V. Alone and Tanaya Kole

16. Thioredoxin regulates the redox state and the activity of the human tRNA ligase complex
Javier Martinez

17. Systematic Identification of RNA-Binding Proteins in Mammalian Tissues
Joel Ignacio Perez-Perri and André Balsinha

18. Protein-Nucleic acids Interaction by NMR
Sai Chaitanya Chiliveri

19. Densitometric Quantification of RNA-Binding Protein Expression in Brightfield Immunohistochemical Tissue Sections
Verónica Fuentes-Santamaría and Juan Carlos Alvarado

20. Engineering 3′ tRNA Termini: Strategies, Protocols, and Applications Using the CCA-Adding Enzyme
Krishna K. Inampudi

21. Epitranscriptomic and RNA-Binding Protein Mechanisms in HIV-Associated Neurocognitive Disorders: From Molecular Regulation to Experimental Approaches
Samikkannu Thangavel and Karthick Chennakesavan

Product details

  • Edition: 1
  • Latest edition
  • Volume: 745
  • Published: March 15, 2027
  • Language: English

About the editors

SC

Subashchandrabose Chinnathambi

Subashchandrabose Chinnathambi is Additional Professor at the Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India. His scientific interests include physiopathology of neurodegenerative disorders, Tau Cytoskeleton, Tau-GPCR, purinergic and chemokine receptors, Tau stem cells, animal models, neuropharmacology, therapeutic approaches targeting Tau oligomers, and biomarkers for neurodegeneration. He serves on the editorial board for various international cell biology journals and is also a regular invited speaker at a number of meetings and workshops. Dr. Chinnathambi has more than 16 years of research experience in the area of neuroscience and Tau-related research in Alzheimer’s Disease.

Affiliations and expertise
Additional Professor, Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India

DC

David Christianson

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.

Affiliations and expertise
Roy and Diana Vagelos Professor in Chemistry and Chemical Biology, Department of Chemistry and Chemical Biology, University of Pennsylvania, Philadelphia, USA

KA

Karen N. Allen

Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering
Affiliations and expertise
Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, Boston, MA, USA