Receptor-Receptor Interactions
- 1st Edition, Volume 117 - October 28, 2013
- Latest edition
- Editor: P. Michael Conn
- Language: English
This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring… Read more
This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes.
With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come.
- Covers sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes
- Chapters are written by experts in the field
- Cutting-edge material
Researchers and students in cell, molecular and developmental biology
Series Page
Contributors
Preface
References
Chapter 1. Spatial Intensity Distribution Analysis (SpIDA): A New Tool for Receptor Tyrosine Kinase Activation and Transactivation Quantification
Abstract
Introduction
1.1 Theory of Spatial Intensity Distribution Analysis
1.2 SpIDA: Examples of Application to RTK
1.3 Procedure for SpIDA
1.4 Discussion
Acknowledgments
References
Chapter 2. Dimerization of Nuclear Receptors
Abstract
Introduction
2.1 Methods
Acknowledgments
References
Chapter 3. Network Analysis to Uncover the Structural Communication in GPCRs
Abstract
Introduction
3.1 Materials
3.2 Methods
3.3 Discussion
Summary
Acknowledgments
References
Chapter 4. Simulating G Protein-Coupled Receptors in Native-Like Membranes: From Monomers to Oligomers
Abstract
Introduction
4.1 Membranes
4.2 GPCR Monomers in Membranes
4.3 GPCR Dimers and Oligomers in Membranes
References
Further Reading
Chapter 5. Structure-Based Molecular Modeling Approaches to GPCR Oligomerization
Abstract
Introduction
5.1 Protein–Protein Docking
5.2 MD Simulation
5.3 Normal Mode Analysis
5.4 Electrostatics Studies
Acknowledgments
References
Chapter 6. Biochemical and Imaging Methods to Study Receptor Membrane Organization and Association with Lipid Rafts
Abstract
Introduction and Rationale
6.1 Methods to Determine Protein Association with Lipid Rafts
6.2 Optical Techniques to Study Receptor Membrane Organization and Association with Lipid Rafts
Concluding Remarks
Acknowledgments
References
Chapter 7. Serotonin Type 4 Receptor Dimers
Abstract
Introduction
7.1 Materials
7.2 Methods
7.3 Discussion
Summary
Acknowledgments
References
Chapter 8. Bioluminescence Resonance Energy Transfer Methods to Study G Protein-Coupled Receptor–Receptor Tyrosine Kinase Heteroreceptor Complexes
Abstract
Introduction
Acknowledgments
References
Chapter 9. A Simple Method to Detect Allostery in GPCR Dimers
Abstract
Introduction and Rationale
Summary
Acknowledgments
References
Chapter 10. Fluorescence Correlation Spectroscopy and Photon-Counting Histogram Analysis of Receptor–Receptor Interactions
Abstract
Introduction
10.1 Materials
10.2 Methods
10.3 Discussion
Summary
Acknowledgments
References
Chapter 11. Monitoring Receptor Oligomerization by Line-Scan Fluorescence Cross-Correlation Spectroscopy
Abstract
Introduction
11.1 Materials
11.2 Methods
11.3 Discussion
Acknowledgments
References
Chapter 12. Biochemical Assay of G Protein-Coupled Receptor Oligomerization: Adenosine A1 and Thromboxane A2 Receptors Form the Novel Functional Hetero-oligomer
Abstract
Introduction
Conclusion
References
Chapter 13. Oligomerization of Sweet and Bitter Taste Receptors
Abstract
Introduction and Rationale
13.1 Materials
13.2 Methods
13.3 Discussion
Summary
Acknowledgments
References
Chapter 14. Analysis of Receptor–Receptor Interaction by Combined Application of FRET and Microscopy
Abstract
Introduction and Rationale
14.1 Theory
14.2 Materials / Experimental Setups
14.3 Methods (Protocols and Procedure)
14.4 Results and Discussion
Acknowledgments
References
Chapter 15. Site-Specific Labeling of Genetically Encoded Azido Groups for Multicolor, Single-Molecule Fluorescence Imaging of GPCRs
Abstract
15.1 Purpose
15.2 Theory
15.3 Protocol 1: Genetically Encoded Azido Groups for Bioorthogonal Conjugation
15.4 Protocol 2: Labeling with Fluorescent Probes Using Cyclooctynes
15.5 Protocol 3: Preparation of Biotinylated Antibodies
15.6 Protocol 4: Preparation of Detergent-Solubilized Lipids
15.7 Protocol 5: Single-Molecule Immunoprecipitation on Glass-Bottom Microplates
15.8 Protocol 6: Automated Multicolor, Single-Molecule TIRF Microscopy
Acknowledgments
References
Chapter 16. Analysis of EGF Receptor Oligomerization by Homo-FRET
Abstract
Introduction
16.1 Theory Homo-FRET Quantification
16.2 Materials
16.3 Methods
16.4 Discussion
Acknowledgments
References
Chapter 17. Detection of G Protein-Coupled Receptor (GPCR) Dimerization by Coimmunoprecipitation
Abstract
Introduction
17.1 Materials
17.2 Methods
17.3 Discussion
Acknowledgments
References
Further Reading
Chapter 18. Lipid-Dependent GPCR Dimerization
Abstract
Introduction
18.1 Materials and Method
18.2 FRET Measurements and Analysis
18.3 Interpreting Results
Summary
References
Chapter 19. Monitoring Peripheral Protein Oligomerization on Biological Membranes
Abstract
Introduction
19.1 Materials and Methods
19.2 Considerations
Summary and Conclusion
Acknowledgments
References
Chapter 20. Single-Molecule Imaging of Receptor–Receptor Interactions
Abstract
Introduction
20.1 Receptor Expression and Fluorescent Labeling
20.2 Single-Molecule Imaging
20.3 Data Analysis
Acknowledgments
References
Chapter 21. Visualization of TCR Nanoclusters via Immunogold Labeling, Freeze-Etching, and Surface Replication
Abstract
Introduction
21.1 Materials
21.2 Equipment
21.3 Methods
21.4 Analysis
21.5 Considerations
Acknowledgments
References
Chapter 22. Identification of Multimolecular Complexes and Supercomplexes in Compartment-Selective Membrane Microdomains
Abstract
Introduction and Rationale
22.1 Detergent-Insoluble Glycosphingolipid (DIG) Microdomain Isolation
22.2 Plasma Membrane Isolation
22.3 Cardiolipin-Enriched Mitochondrial Membrane Microdomain Isolation
22.4 Mitochondrial Supercomplex Identification By Blue-Native Gel Electrophoresis
22.5 Discussion
Summary
Acknowledgments
References
Chapter 23. G Protein-Coupled Receptor Transactivation: From Molecules to Mice
Abstract
Introduction
23.1 Materials
23.2 Methods
23.3 Discussion
Summary
Acknowledgments
References
Chapter 24. Crystallization of G Protein-Coupled Receptors
Abstract
Abbreviations
Introduction
24.1 Crystallization of Bovine Rhodopsin
24.2 Crystallization of β2-AR
24.3 Discussion
Acknowledgments
References
Index
Volumes in Series
- Edition: 1
- Latest edition
- Volume: 117
- Published: October 28, 2013
- Language: English
PC
P. Michael Conn
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
Affiliations and expertise
Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center, TX, USARead Receptor-Receptor Interactions on ScienceDirect