Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology
- 1st Edition, Volume 650 - April 15, 2021
- Imprint: Academic Press
- Editor: Joseph A. Cotruvo
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 3 8 5 6 - 1
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 3 8 5 7 - 8
Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophy… Read more
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Request a sales quoteMethods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter One: Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs
- Abstract
- 1: Introduction
- 2: Screening for Ln-responsive microorganisms
- 3: Discovery of Ln-dependent MDH and Ln-dependent methylotrophy
- 4: Screening for Ln-dependent methylotrophs
- 5: Summary
- Acknowledgment
- Chapter Two: Synthetic modeling of the structure and function of the rare-earth dependent methanol dehydrogenase cofactor
- Abstract
- 1: Introduction
- 2: Ligand design and realization
- 3: LQQ—Synthetic pathway and purification
- 4: Structure and reactivity of [La(LQQ)(NO3)3]
- 5: Summary
- 6: Experimental section
- Acknowledgments
- Chapter Three: Activity assays of methanol dehydrogenases
- Abstract
- 1: Assays based on artificial electron acceptors
- 2: Assays based on the native cytochrome electron acceptor
- 3: Role of activators, additives, and buffers
- 4: Safety considerations
- Chapter Four: Expression, purification and properties of the enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, and XoxG4
- Abstract
- 1: Introduction
- 2: Expression hosts and expression conditions
- 3: Protein purification and analysis protocols
- 4: Enzyme properties
- 5: Functional characterization of XOXG4
- 6: Summary
- Acknowledgment
- Chapter Five: Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1
- Abstract
- 1: Introduction: A platform for expression of Ln3 +-dependent enzymes
- 2: Expression of Ln3 +-dependent enzymes
- 3: Purification of recombinant Ln3 +-dependent enzymes
- 4: Growth phenotyping with Ln3 +-dependent enzymes
- 5: Final remarks
- Chapter Six: Heterologous expression, purification, and characterization of proteins in the lanthanome
- Abstract
- 1: Introduction
- 2: Purification and assay of native XoxF from M. extorquens AM1
- 3: XoxG
- 4: XoxJ
- 5: Lanmodulin
- 6: PqqT
- 7: Summary
- Acknowledgments
- Chapter Seven: Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system
- Abstract
- 1: Introduction
- 2: General scheme for ISphoA/hah-Tc mutagenesis
- 3: Transposon mutagenesis studies used to identify gene products involved in Ln and methanol metabolism
- 4: Materials and methods
- 5: Concluding remarks
- Acknowledgments
- Chapter Eight: Metals and methylotrophy: Via global gene expression studies
- Abstract
- 1: Introduction: Metals and methylotrophy
- 2: Gene expression studies: Experimental design
- 3: Gene regulatory networks: Overview
- 4: Final remarks
- Acknowledgments
- Chapter Nine: Mass spectrometry-based approaches to study lanthanides and lanthanide-dependent proteins in the phyllosphere
- Abstract
- 1: Introduction
- 2: Cultivation of A. thaliana and harvest of the phyllosphere
- 3: Sample preparation
- 4: Mass spectrometry acquisition parameters
- 5: General experimental considerations
- 6: Summary
- Acknowledgment
- Chapter Ten: Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph “Methylotuvimicrobium buryatense” 5GB1C
- Abstract
- 1: Introduction
- 2: Methods
- 3: Conclusion
- Edition: 1
- Volume: 650
- Published: April 15, 2021
- No. of pages (Hardback): 276
- No. of pages (eBook): 276
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780128238561
- eBook ISBN: 9780128238578
JC
Joseph A. Cotruvo
Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry
Pennsylvania State University, PA, USA
Affiliations and expertise
Assistant Professor, Pennsylvania State University, PA, USARead Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology on ScienceDirect