Radiation Oncology and Radiotherapy Part B
- 1st Edition, Volume 174 - January 31, 2023
- Editors: Ai Sato, Jeffrey Kraynak, Ariel E. Marciscano, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 8 9 9 4 7 - 5
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 9 9 4 8 - 2
Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesti… Read more
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Request a sales quoteRadiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Cytofluorometric characterization of the lymphoid compartment of irradiated tumors, Cytofluorometric characterization of the myeloid compartment of irradiated tumors, Mass cytometry to characterize the immune infiltrate of irradiated tumors, Characterization of the immune infiltrate in irradiated mouse tumor by multiplex immunofluorescence, and much more.
Other chapters cover Methods to study FOXP3+CD8+ cells in irradiated bone metastases, Luminex-based characterization of cytokine signaling by irradiated tumors, Explant-based assessment of anticancer immunity in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Academic, researchers, government and industrial sectors
- Cover image
- Title page
- Table of Contents
- Series page
- Copyright page
- Contributors
- Radiation therapy: An old dog learning new tricks
- References
- Chapter 1: Flow cytometry-assisted quantification of immune cells infiltrating irradiated tumors in mice
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 FACS analysis and TIL quantification
- 5 Timing of the sampling
- 6 Notes
- 7 Concluding remarks
- Disclosures
- Acknowledgements
- References
- Chapter 2: Cytofluorometric characterization of the myeloid compartment of irradiated mouse tumors
- Abstract
- 1 Introduction
- 2 Materials
- 3 Cell culture
- 4 Oral tumor implantation
- 5 In vivo imaging
- 6 Irradiation
- 7 Flow cytometry analysis
- 8 Concluding remarks
- 9 Notes
- Disclosures
- Acknowledgments
- References
- Chapter 3: Mass cytometry to characterize the immune lung cancer microenvironment
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- 5 Concluding remarks
- Acknowledgments
- References
- Chapter 4: Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence
- Abstract
- 1 Introduction
- 2 Multiplex immunofluorescence staining
- 3 Materials
- 4 Methods
- 5 Multiplex imaging
- 6 Conclusion
- Acknowledgements
- References
- Chapter 5: Ex vivo analysis of radiation effects on tumor infiltrating immune cells using tumor explants
- Abstract
- 1 Introduction
- 2 Materials
- 3 Cancer cell culture and in vivo tumor establishment
- 4 Tumor explant processing
- 5 Cell surface staining and viability determination
- 6 Flow cytometry: Running samples and analysis protocol
- 7 Notes
- Acknowledgements
- References
- Chapter 6: Assessment of immunological memory formation in vivo
- Abstract
- 1 Introduction
- 2 Material
- 3 Method
- 4 Notes
- 5 Concluding remarks
- References
- Chapter 7: MorphoMacro for in vivo and ex vivo quantitative morphometric analysis of microglia
- Abstract
- 1 Introduction
- 2 Materials and methods
- 3 Expected results
- 4 Notes
- 5 Concluding remarks
- Funding
- Acknowledgments
- References
- Chapter 8: Quantitative assessment of mitophagy in irradiated cancer cells
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Notes
- 5 Concluding remarks
- Acknowledgments
- Conflicts of interest
- References
- Chapter 9: Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Concluding remarks
- Conflict of interest statement
- References
- Chapter 10: A simple method to assess clonogenic survival of irradiated cancer cells
- Abstract
- 1 Introduction
- 2 Materials
- 3 Establishment of B16-OVA cell line and radiotherapy
- 4 Concluding remarks
- Disclosures
- References
- Chapter 11: Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy
- Abstract
- 1 Introduction
- 2 Materials
- 3 Cell seeding for clonogenic assay
- 4 Cell irradiation using 223RaCl2
- 5 Determination of clonogenic cell survival in donor and recipient cells
- 6 Concluding remarks
- 7 Notes
- References
- No. of pages: 176
- Language: English
- Edition: 1
- Volume: 174
- Published: January 31, 2023
- Imprint: Academic Press
- Hardback ISBN: 9780323899475
- eBook ISBN: 9780323899482
AS
Ai Sato
Ai Sato is a research specialist at Stich Radiation Oncology, New York, NY, USA
Affiliations and expertise
Stich Radiation Oncology, New York, NY, USAJK
Jeffrey Kraynak
Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.
Affiliations and expertise
research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.AM
Ariel E. Marciscano
Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College.
Affiliations and expertise
Assistant Professor of Radiation Oncology, Weill Cornell Medical CollegeLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead Radiation Oncology and Radiotherapy Part B on ScienceDirect