Radiation Oncology and Radiotherapy, Part A
- 1st Edition, Volume 172 - September 1, 2022
- Imprint: Academic Press
- Editors: Ai Sato, Jeffrey Kraynak, Ariel E. Marciscano, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 8 9 9 4 9 - 9
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 9 9 5 0 - 5
Radiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chap… Read more
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Request a sales quoteRadiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more.
Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2α phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Academic, researchers, government and industrial sectors
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Radiation therapy: An old dog learning new tricks
- Acknowledgments
- Conflict of interest
- References
- Chapter 1: Cytofluorometric assessment of cell cycle progression in irradiated cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- Competing interests
- References
- Chapter 2: Cytofluorometric assessment of acute cell death responses driven by radiation therapy
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- Conflicts of interest
- References
- Chapter 3: Assessment of lipid peroxidation in irradiated cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- Acknowledgments
- Disclosures
- References
- Chapter 4: Biochemical, genomic, and epigenomic profiling of isolated cancer cell lines’ micronuclei
- Abstract
- 1: Introduction
- 2: Materials (see note 1)
- 3: Cell maintenance, preparation, and nuclei isolation
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- Disclosures
- References
- Chapter 5: Assessment of transcription inhibition as a characteristic of immunogenic cell death
- Abstract
- 1: Introduction
- 2: Protocol A: Colocalization between Fibrillarin (FBL) and Nucleolin (NCL)
- 3: Protocol B: Click-iT® RNA HCS assay
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- Conflict of interest
- References
- Chapter 6: Assessment of eIF2α phosphorylation during immunogenic cell death
- Abstract
- 1: Introduction
- 2: Material
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- References
- Chapter 7: Interference of immunogenic chemotherapy by artificially controlled calreticulin secretion from tumor cells
- Abstract
- 1: Introduction
- 2: Materials and reagents
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Acknowledgments
- References
- Chapter 8: Quantification of cytosolic DNA species by immunofluorescence microscopy and automated image analysis
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- Conflicts of interest
- References
- Chapter 9: Assessment of type I interferon responses as a feature of immunogenic cell death
- Abstract
- 1: Introduction
- 2: Material
- 3: Method
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- Conflict of interest
- References
- Chapter 10: RT-PCR-assisted quantification of type I IFN responses in irradiated cancer cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- Competing interests
- References
- Chapter 11: ELISA-based quantification of type I IFN secretion by irradiated cancer cells
- Abstract
- 1: Introduction
- 2: Material
- 3: Method
- 4: Concluding remarks
- Conflict of interest statement
- References
- Chapter 12: Assessment of hypoxia by pimonidazole staining following radiotherapy
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- Disclosures
- References
- Edition: 1
- Volume: 172
- Published: September 1, 2022
- No. of pages (Hardback): 214
- No. of pages (eBook): 214
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780323899499
- eBook ISBN: 9780323899505
AS
Ai Sato
Ai Sato is a research specialist at Stich Radiation Oncology, New York, NY, USA
Affiliations and expertise
Stich Radiation Oncology, New York, NY, USAJK
Jeffrey Kraynak
Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.
Affiliations and expertise
research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.AM
Ariel E. Marciscano
Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College.
Affiliations and expertise
Assistant Professor of Radiation Oncology, Weill Cornell Medical CollegeLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead Radiation Oncology and Radiotherapy, Part A on ScienceDirect