Quantitative Gas Chromatography-Mass Spectrometry in Clinical and Pharmacological Analysis

Quantitative Gas Chromatography-Mass Spectrometry in Clinical and Pharmacological Analysis: Principles and Applications in Lipids and Biomarkers is an essential resource for researchers, clinicians, and analytical scientists involved in clinical and pharmacological analysis. It provides practical guidance on using gas chromatography-mass spectrometry (GC-MS) and tandem mass spectrometry (GC-MS/MS) for the accurate quantification of physiological substances such as lipids, fatty acids, bile acids, eicosanoids, and biomarkers of oxidative stress like malondialdehyde and isoprostanes. Emphasizing the use of stable isotope-labelled internal standards and chemical ionization techniques, the book enables sensitive and precise measurement of analytes in complex biological samples like blood and urine, supporting disease diagnosis, therapy monitoring, and biomedical research. Designed with a focus on real-world application, it offers step-by-step procedures, troubleshooting tips, and illustrative diagrams to aid understanding. The content covers sample preparation, method validation, and quality control, making it a practical reference for laboratory scientists. Additionally, it highlights recent developments and biomedical applications, providing current knowledge and innovative approaches in the field. Overall, this comprehensive manual bridge analytical techniques with clinical and pharmacological needs, facilitating accurate biomarker analysis to improve health outcomes and scientific understanding.