Protocols and Applications in Enzymology
- 1st Edition - November 24, 2021
- Latest edition
- Authors: Seema Anil Belorkar, Sudisha Jogaiah
- Language: English
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrumen… Read more
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.
- Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards
- Presents easy to read, reproducible protocols for researchers and students across academia and industry
- Includes color diagrams that illustrate key concepts
Biochemists, molecular biologists, cellular biologists, geneticists, enzymologists, chemists and biochemical engineers. Students and clinicians across the life sciences; researchers across academia and industry
1. Introduction Enzymes-Past, present and Future
2. Distribution and diversity in microbial enzymes
3. Screening of potential microbes for enzymes of industrial significance
4. Solid state fermentations and SMF for enzyme production
5. Laboratory methods for enzyme assays
6. Structure and functions of enzyme kinetics
7. Protocols of important industrial enzymes
8. Strategies to improve enzyme activity for industrial processes
9. Scope and relevance of Industrial applications
10. Agro-industrial wastes for enzyme production
11. Significance of enzymes and their agricultural applications
12. Biotechnological applications of enzymes and future prospective
13. Conclusions
- Edition: 1
- Latest edition
- Published: November 24, 2021
- Language: English
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Seema Anil Belorkar
Dr. Seema Anil Belorkar is Assistant Professor at the PG Department of Microbiology and Bioinformatics, Atal Bihari Vajpayee University, Bilaspur, with expertise in bioactive molecule production and enzymology. She has published 20 publications in various national and international journals, and has successfully completed 4 research projects with involvin production of enzymes and their application in agro-industrial wastes. Dr. Belorkar has contributed to 4 chapters in various books published by Springer, Wiley-Blackwell, and Elsevier. She has received 3 awards in the field of teaching and research, and has been a reviewer for articles in BMC Plant Biology, the Indian journal of Pharmaceutical Sciences and other journals of repute.
Affiliations and expertise
Assistant Professor, Microbiology and Bioinformatics Department, Atal Bihari Vajpayee University, Bilaspur (C.G), IndiaSJ
Sudisha Jogaiah
Dr. Sudisha Jogaiah is a Fellow of the National Academy of Sciences (FNASc), the Agricultural Sciences (FNAAS), and the National Academy of Biological Sciences (FNABS). He is also a Bridge Fellow, Government of Japan, and serves as a Visiting Professor at Yamaguchi University, Japan, and Nanjing Agricultural University, China. In 2022, he joined the Department of Environmental Science at the Central University of Kerala as an Associate Professor. He has been listed among the World's Top 2% of Scientists by Stanford University for 3 consecutive years. His research interests include plant pathology, plant–microbe interactions, plant defense signaling, biocontrol agents, biogenic nanoparticles for induced resistance in plants, and bioremediation. The technological quality and scientific rigor of his laboratory are evident from his extensive publication record and the prestigious national and international awards received.
Affiliations and expertise
Professor, Department of Environmental Science, Central University of Kerala, Kasaragod, Kerala, IndiaRead Protocols and Applications in Enzymology on ScienceDirect