Protocols and Applications in Enzymology

1st Edition - November 19, 2021
Authors: Seema Anil Belorkar, Sudisha Jogaiah
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 2 6 8 - 6
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 4 3 7 - 6

Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrumen… Read more

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Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.

Cover image
Title page
Table of Contents
Copyright
Preface
Chapter 1. Enzymes—past, present, and future
1.1. General introduction
1.2. Characteristics of enzymes
1.3. The concept of cell-free fermentation
1.4. Nomenclature of enzymes
1.5. Enzymes—the present scenario
1.6. Recent developments
1.7. The future
Chapter 2. Distribution and diversity in microbial enzymes
2.1. Origin of enzyme diversity
2.2. Natural niche as a diversity source
2.3. Evolution and function of enzymes
2.4. Acidophilic enzymes
2.5. Alkaliphiles
2.6. Halophilic organisms
2.7. The protist—a novel enzyme source
2.8. Future directions
Chapter 3. Screening of potential microbes for enzymes of industrial significance
3.1. History
3.2. High throughput screening methods—need of present day
3.3. Throughput methods for screening of enzyme variants
3.4. Conclusion and future perspective
Chapter 4. Solid-state fermentation and submerged fermentation for enzyme production
4.1. History of fermentation for social benefits
4.2. Louis Pasteur and fermentation
4.3. Fermentation
4.4. Conclusion and future directions
Chapter 5. Laboratory methods for enzyme assays
Subchapter 5.1. Assay of enzyme—fructosyltransferase
Subchapter 5.2. Assay of enzyme—Lipase
Subchapter 5.3. Assay of enzyme—protease
Subchapter 5.4. Assay of enzyme—xylanases
Chapter 6. Structure and functions of enzyme kinetics
6.1. Introduction
6.2. Gibb's free energy and enzyme kinetic
6.3. The catalyst
6.4. Enzyme kinetics and features of ECR
6.5. The transition complex in enzyme-catalyzed reaction
6.6. The Michaelis–Menten equation
6.7. Advantages
6.8. Disadvantages
6.9. The significance of Km and Vmax
6.10. Conclusion
Chapter 7. Protocols of important industrial enzymes
Subchapter 7.1. Large-scale production of enzyme—fructosyltransferase
Subchapter 7.2. Assay of enzyme—xylanases
Subchapter 7.3. Large-scale production of enzyme—lipase
Subchapter 7.4. Large-scale production of protease
Subchapter 7.5. Small-scale production of enzyme—fructosyltransferase
Subchapter 7.6. Small-scale production of enzyme—lipase
Subchapter 7.7. Small-scale production of protease
Subchapter 7.8. Assay of enzyme—xylanases
Chapter 8. Strategies to improve enzyme activity for industrial processes
8.1. Introduction
8.2. Conclusion
Chapter 9. Scope and relevance of industrial applications
9.1. Introduction
9.2. Enzymes in industries
9.3. Common strategies for enzyme immobilization
9.4. Some important industrial enzymes
9.5. Biocatalytic membranes
9.6. Conclusion
Chapter 10. Agroindustrial wastes for enzyme production
10.1. Introduction
10.2. Agroindustrial wastes: excellent raw material for production
10.3. Impact of agroindustrial wastes on environment: a global concern
10.4. Waste to value
10.5. Fructosyltransferase
10.6. Xylanase
10.7. Conclusion
Chapter 11. Significance of enzymes and their agricultural applications
Subchapter 11.1. Introduction
Subchapter 11.1. Production of enzyme biofertilizer—phosphatase
Chapter 12. Biotechnological applications of enzymes and future prospective
12.1. Introduction
12.2. Enzymes in medical field
12.3. The biological detergents: a revolution in laundry industry
12.4. Conclusion
Chapter 13. Conclusion
Index

Seema Anil Belorkar

Dr. Seema Anil Belorkar is Assistant Professor at the PG Department of Microbiology and Bioinformatics, Atal Bihari Vajpayee University, Bilaspur, with expertise in bioactive molecule production and enzymology. She has published 20 publications in various national and international journals, and has successfully completed 4 research projects with involvin production of enzymes and their application in agro-industrial wastes. Dr. Belorkar has contributed to 4 chapters in various books published by Springer, Wiley-Blackwell, and Elsevier. She has received 3 awards in the field of teaching and research, and has been a reviewer for articles in BMC Plant Biology, the Indian journal of Pharmaceutical Sciences and other journals of repute.

Affiliations and expertise

Assistant Professor, Microbiology and Bioinformatics Department, Atal Bihari Vajpayee University, Bilaspur (C.G), India

Sudisha Jogaiah

Dr. Sudisha Jogaiah, is the Associate Professor and Head of the Department of Environmental Science at the Central University of Kerala. Dr. Jogaiah is one of the leading scientists in dissecting the molecular mechanisms of rhizosphere fungi mediated Induce systemic resistance (ISR) underlying SA/JA/ET cross talk and its consequent effect on commercial biocontrol strategies in India. He is the Fellow of The National Academy of Sciences (FNASc.), Fellow of The National Academy of Agricultural Sciences (FNAAS.), Fellow of National Academy of Biological Sciences (FNABS), His research emphasizes plant–microbe interactions and eco-friendly crop protection, contributing to advances in sustainable agricultural practices. The vision of Dr. Jogaiah research offers a fresh perspective to agriculture, horticulture, and agro-ecosystems from “Laboratory to Land” and is designed to educate and train the farmers for implementation of sustainable crop production. Dr. Jogaiah has played active roles in both national and international academic circles and has an extensive record of scholarly publications and contributions to authored books and edited volumes. His work supports a deeper understanding of environmental science and its practical applications in sustainable agriculture.

Affiliations and expertise

Assistant Professor, Laboratory of Plant Healthcare and Diagnostics, PG Department of Studies in Biotechnology and Microbiology, Karnatak University, Pavate Nagar, Dharwad, Karnataka, India

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